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Background and Objective: Para-nonylphen as an environmental pollutant has weak estrogenic activity and causes oxidative stress in different organs including testis. This study was done to determine the protective effect of vitamin E on the para-nonylphenol induced-testicular toxicity in adult rats. Methods: In this experimental study, 24 Wistar rats were randomly allocated into four groups including control, vitamin E (100 mg/kg/day, orally), para-nonylphenol (250mg/kg/day, orally) and finally para-nonylphenol (250mg/kg/day, orally) plus vitamin E (100mg/kg/day, orally). After 56 days of treatment, removal of the right testis, tissue processing and staining with Heidenhain's Azan, the morphometric parameters of testicular tissue was evaluated using stereological method. Results: The mean volume of seminiferous tubules, height of the germinal epithelium, seminiferous tubules diameter, thickness of the basement membrane, number of spermatocyte, spermatid and sertoli cells significantly reduced in para nonylphenol group compared to the controls (P<0.05). These parameters were significantly increased in the para-nonylphenol plus vitamin E group compared to para nonylphenol group (P<0.05). In the histopathological examination, atrophy of seminiferous tubules, germinal epithelium vacuolation and epithelial disarrangement were observed in para nonylphenol group. Histopathological alterations reduced in para-nonylphenol plus vitamin E group compared to para nonylphenol group. Conclusion: Co-administration of vitamin E with para nonylphenol can prevent the adverse effects of para nonylphenol on the testicular tissue in adult rats.

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Background and Objective: Sodium Arsenite is an environmental pollutant which can generate free radicals causing tissue damage. This study was done to evaluate the effect of Green Tea (GTE), as a strong antioxidant, on kidney tissue in mice treated with Sodium Arsenite. Methods: In this experimental study 24 adult male NMRI mice were randomly allocated into four groups including: control, GTE (100mg/kg/day), Sodium Arsenite (5mg/kg/day) and Sodium Arsenite + GTE, for 34 days, orally. Animals were scarified and left kidney was taken out, fixed, sectioned, processed and stained using Heidenhain'azan method. Using stereological technique the total volume of kidney, volume of cortex, medulla, proximal and distal tubule, renal corpuscle, gelomerelus, tuft and capillary, membrane and space of Bowman's capsule and length of proximal and distal tubule were determined. Creatinine, BUN and MDA serum samples were measured. Results: The mean of total volume of cortex, proximal tubule, distal tubule, renal corpuscle and gelomerolus, taft, Bowman's capsule space, size of epithelium and lumen of proximal and distal tubule were significantly reduced in Sodium Arsenite group compared to control (P<0.05). These parameters were significantly increased in the Sodium Arsenite + GTE group in comparison with Sodium Arsenite group (P<0.05). The creatinine, Blood urea nitrogen (BUN) and MDA were significantly increased in the Sodium Arsenite group in compared to the control group (P<0.05). These parameters were significantly reduced in the Sodium Arsenite + GTE group in comparison with Sodium Arsenite group (P<0.05). Conclusion: Green tea has a protective role in Sodium Arsenite induced nephrotoxicity.

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Background and Objective: The degeneration of motor neuron in anterior horn of spinal cord can be caused by compression. Hyssopus officinalis of Laminacea family demonstrate antioxidant and anti-inflammation effects. This study was done to evaluate the effect of alcoholic extract of Hyssopus officinalis leaves, on motor neuron in spinal cord after sciatic nerve compression in male rats.

Methods: In this experimental research, 60 male wistar rats were randomly allocated into six groups including; control, compression, and compression + treatment (25, 50, 75, 100 mg/kg/bw). In order to induce compression, sciatic nerve of right leg was exposed to compression for 60 second using locker pincers. Extract injected intraperitoneally in the first and second week after compression. 28 days after compression under profusion method, the lumber spinal cord was sampled. The density of motor neurons (9-20 micron) was measured using dissector and stereological method.

Results: Density of neurons in compression group (611±34) significantly reduced compared to the control group (1658±30) (P<0.05). Moreover, neuronal density was significantly increased in
25 (1179±22), 50 (1260±20), 75 (1350±15) and 100 (1120±10) mg/kg/bw doses in treatment groups in compared to the compression group (P<0.05).

Conclusion: Alcoholic extract of Hyssopus officinalis leaves exhibite neuroprotective effect on neurons in anterior horn of the spinal cord after injury. This effect probably is related to the antioxidant and anti inflammation properties in alcoholic extract of Hyssopus officinalis, dose dependly.

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Background and Objective: Bisphenol A (BPA) is an endocrine disruptor chemical and as an environmental pollutant is able to generate free radicals causing tissue damage. This study was done to investigate the effect of Nigella sativa oil against BPA induced toxicity on the tissue of male NMRI mice kidney by stereological method.

Methods: In this experimental study 24 adult male NMRI mice (32±3 g) were randomly allocated into control, BPA (200 mg/kg/day), BPA (200 mg/kg/day) plus Nigella sativa oil (5 ml/kg/day) and Nigella sativa oil (5 ml/kg/day) groups and treated for 5 weeks, orally. At the end, animals were sacrificed, their left kidneys were removed, fixed, sectioned, processed and stained with Heidenhain' azan staining method. Then, the kidney tissue sections were evaluated using stereological method and serum malondialdehyde (MDA) level was also measured.

Results: The total weight and volume of kidney, volume of cortex, volume of proximal and distal tubules and volume of their lumen, volume of interstitial tissue, volume of glomeruli, tuft, as well as serum MDA level significantly increased in BPA treated group compared to the controls (P<0.05). These parameters were significantly reduced in BPA plus Nigella sativa oil group compared to BPA ones (P<0.05).

Conclusion: This study revealed that Nigella sativa oil can reduce the oxidative stress toxicity induced by BPA in the mice renal tissue.