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Background&Objective: Up to seventh week of gestation, progesterone secreted from the ovary is necessary to preserve the pregnancy. In the ART cycles GnRH-a is prescribed and oocytes and granulosa cells or retrieved, both causes corpus luteum insufficiency, so corpus luteum should be supported the best way is progesterone prescription. In this research we compare intramuscular and intravaginal suppositoar forms of progesterone for preserve the pregnancy. Materials&Methods: A prospective cross sectional evaluation on 193 patients was designed. All of them were on IVF or ICSI cycles. The patients were randomly divided into two groups. Intramuscular progesterone was prescribed in 138 and intravaginal progesterone (supp cyclogest) in 54 patients. The outcome of pregnancy was analyzed using statistical trials including t-test and chi-squre. Results: The two groups were similar duo to age – the ethiology causes of infertility – the doses of proscribed HMG ampule – FSH titrago – the number and grade of transferred embryos. The day after HCG presciption until the end the seventh week in the first group (138 patients) intramuscular progestrone (50 mg /bid) and in the secound group (54 Patients) supp of cyclogest (400 mg / bid) was prescnbed. The rate of positive pregnancy test, abortion, successful poegnancy in this two groups were similar. Conclusion: Intramuscular or intravaginal progesterone for supporting corpus luteum in IVF or ICSI cycles has the same effect but the intramuscular route is cheaper.

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Background and Objective: Success rate of IVF depends on infertility factors and its treatment cycle. Different methods of treatment and different centers have reported various rates of success. The aim of this study was to evaluate the fertility outcome after IVF and related factors. Materials and Methods: This descriptive analytic study was performed on 150 infertile couples following IVF in Montaserieh Infertility Center, Mashhad University of Medical Sciences, Mashhad, North – East of Iran during 2001-06. Data was collected by a questionnaire including woman's age, husband's age, infertility factor, spermogram, infertility period, the number of follicles caused by treatment, the number of oocytes, the number of produced and transferred fetus and treatment outcomes. Data was analyzed by SPSS software and Chi-square, t-studen test, One-way ANOVA and logistic regression. Results: The mean age of women was 29.7±5.4. The mean±SD follicles oocytes and transferred fetus were 11±6, 6±3.9 and 2.6±1.5 respectively. There was significant relation between the number of follicles (P<0.05), obtained oocytes (P<0.05) and transfered fetus (P<0.05) with the success rate of IVF. The fertility rate was 24.6%. There was no significant relation between the age groups and fertility rate. Conclusion: This study showed that ovarian response to ovulation stimulation and the number of transferred fetus are the important and effective factors for predicting the outcomes of IVF.

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Background and Objective: Estradiol plays an important role in folliculogenesis and its developmental stages of embryo. This study was done to determine the quantitative assessment of mouse embryo development yielded from in vitro fertilization of ovulated mature oocytes after ovarian stimulation using human menopausal gonadotropin (HMG) and Estradiol valerate (E2). Materials and Methods: In this experimental study, 40 female NMRI mice were allocated into two groups. Control and treatment groups received HMG alone (10 IU/mouse) and a combination of HMG and E2 (1μg/mouse) in single dose manner, respectively. Following the induction of ovulation by HCG, the oocytes collected and morphologically evaluated. MΙΙ oocytes for in vitro fertilization (IVF) were transferred into medium containing capacitated and incubated sperm derived from male NMRI mice. The yielded embryos subsequently transferred into developmental medium for reaching to the blastocyst stage. Results: The difference between the mean percentage of yielded oocytes and healthy MII oocytes in the control and treatment groups was not significant. The percentages of the fertilized oocytes reached to two-cells was 34.22±21.87 and 36.83±20.68 in control and treatment groups, respectively. The percentages of the blastocys stages of embryos was 49.41±26.5 and 62.02±30.11 in control and treatment groups, respectively. Conclusion: The addition of estradiol to HMG as an ovarian stimulator can not increase the rates of yielded MII oocytes and embryonic development.