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Showing 2 results for Hcv
Gholamreza Roshandel, Shahryar Semnani, Nafiseh Abdolahi, Sima Besharat, Abbas Ali Kashtkar, Hamid Reza Joshaghani, Abdolvahab Moradi, Khodaberdi Kalavi, Saba Besharat, Ali Jabbari, Mohammad Javad Kabir, Seyed Ahmad Hosseini, Seyed Mahdi Sedaghat, Danyal Roshandel, Ahmad Danesh,
Volume 9, Issue 4 (12-2007)
Abstract
Background & Objective: Hepatitis B virus (HBV) infection is one of the most important health problems all over the world. This study was conducted to determine the co-infection of hepatitis C virus (HCV), hepatitis D virus (HDV) in HBV infected patients in Golestan province of Iran. Materials & Methods: This population based cross sectional study was done in 2004. 139 HBsAg positive cases were assessed for co-infection with HCV and HDV. Serologic tests were done by ELISA method using available kits. Data were analyzed by SPSS-12 and STATA8 software. P-values of less than 0.05 were considered as significant. Results: Sixty eight (48.9%) of 139 cases were males and 71 (51.1%) were females. The mean (±SD) of participant's age was 41.89 (±11.3) years (range: 25-64 years). Anti-HCV and Anti-HDV antibodies were positive in 17 (12.3%) and 8 (5.8%) cases, respectively. Females were more infected with HCV and HDV than males. But the differences were not significant. Conclusion: The prevalence of co-infection with HCV and HDV in HBV infected cases is relatively high and notable in Golestan province of Iran. Because of negative effects of these co-infections in clinical outcome of the disease, it is recommended to assess possible HCV and HDV infection in individuals with HBV infection. This may result in more efficient management of the disease.
Paryan M (msc), Mohammadi-Yeganeh S (msc), Mondanizadeh M (msc), Khansarinejad B (msc),
Volume 14, Issue 1 (3-2012)
Abstract
Background and Objective: HIV-1 and HCV infections especially in co-infected forms are among the most important infections transferred during blood transfusion.The screening of the blood products is valuable for preventing the transmission of infections. The aim of this study was to evaluate multiplex RT-PCR assay for detection of Co-infection HIV-1 and HCV Viruses in plasma samples.
Materials and Methods: This laboratory study was done to evaluate the use of multiplex RT-PCR assay for simultaneous detection of HIV-1 and HCV genomes in plasma samples. The amplified genomes were detectable in 3% agarose gel base on difference in the numbers of nucleotides. The sensitivity and specificity of this assay was determined on healthy and infected subjects whome simultanously exhibit HIV-1 and HCV co-infection using plasma samples.
Results: The specificity results showed that the primers used in this assay have no interaction with each other and other possible interfering agents. The clinical sensitivity and specificity of the assay has been considered as 90% and 100%, respectively.
Conclusion: Multiplex RT-PCR can be used for screening of blood donors due to high sensivity and specificity.
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