|
|
|
 |
Search published articles |
 |
|
Showing 8 results for Candida
Hosseini Ss (bsc), Roudbar Mohammadi Sh (phd), Joshaghani Hr (phd), Eskandari M (msc),
Volume 12, Issue 4 (12-2010)
Abstract
Background and Objective: The Candida albicans is an opportunistic fungi that can be pathogenic in patients suffering from diabetes and AIDS. This organism can cause various infections such as superficial of the skin and mucosa to deep tissue infections. In this study the antifungal effects of ZnO and SDS on Candida albicans in comparison with Fluconazole were investigated. Materials and Methods: This was an experimental study which evaluated the antifungal effects of biocide SDS and ZnO on Candida albicans by microbroth dilution assay in broth and agar medium. Minimum Inhibitory Concentration (MIC) was determinated for each inhibitor during colony count in comparison with control. Results: MIC of ZnO was 1.013-296 µg/ml and for SDS and Fluconazole were 0.001-0.56 and 0.062-128 µg/ml respectively. Conclusion: This study demonstrated antifungal activity of ZnO can be a candidates for the elimination of candida in medicine particular in medical instruments.
Nowrozi H, Kazemi A, Teshfam M, Temorian Sh, Adimi P, Bashashati M,
Volume 15, Issue 4 (12-2013)
Abstract
Background and Objective: Ultraviolet (UV) radiation is a important disinfectant. Fungal infections with resistant isolates in patients culminate in recurrence of disease even with worse condition. This study was done to evaluate the efficacy of ultraviolet radiation on drug susceptibility of Candida Spp. to itraconazole, fluconazole and amphotericin B. Materials and Methods: This laboratory study was done on 12 Candida spp. isolated from patients according to NCCLS M27- A method. Samples were suspended with sterile saline and optical density was read by spectrophotometer at the wavelength of 530 nm. Serial dilutions (0.0313-16 µg/ml) and (0.0313-128 µg/ml) were supplied for itraconazole, amphotericin and fluconazole, respectively. MICs were determined after 48h incubation at 35°C. Following UV radiation for 1, 2, 5, 10, 60, 90 and 120 seconds MICs were determined, subsequently. Results: The highest MIC pre UV radiation was (>128 µg/ml) for fluconazole. After UV radiation, MICs were steadily decreased for all mentioned drugs while after 10 sec, MICs of itraconazole and amphotericin B were >0.0313 µg/ml. Secondary MICs significantly decreased with respect to MICs obtained in pre UV radiation (P<0.05). Conclusion: UV radiation reduces MICs of Candida spp. to itraconazole, fluconazole, amphotericin B.
Zia Ma, Bayat M, Khalkhali H, Saffari S,
Volume 15, Issue 4 (12-2013)
Abstract
Background and Objective: Candida albicans is the most frequent etiological agent of oral candidiasis. This study was done to compare the anticandidal effect of Thymus vulgaris and Myrtus communis to nystatin on Candida albicans. Materials and Methods: In this laboratory study thirty-two strains of Candida albicans isolated from patients with oral candidiasis. Yeast suspension of Candida yeast cells was provided, subsquntly a serial dilution from Thymus vulgaris and Myrtus communis and Nystatin in Sabouraud Dextrose Agar (SDA) medium were prepared. Then a loop of Candida suspension was cultured on all of the solid media and was incubated at 25°C. The findings of fungus growing were recorded during 7 days. Results: MIC of Thymus vulgaris, Myrtus communis L, mix of these essences and Nystatin was 0.390µl/ml, 12.5 µl/ml, 0.78 µl/ml and 160 IU/ml, respectively. Conclusion: Thymus vulgaris contained antifungal activity against Candida albicans, but Myrtus communis demonstrated a very low activity against Candida albicans.
Ebrahimzadeh A, Mousavi M,
Volume 16, Issue 3 (10-2014)
Abstract
Background and Objective: Fungal ear infection is common diseases in tropical areas with manifestation of acute and chronic clinical symptoms in external ear canal. This study was done to compare the fungal flora in external ear canal in chronic otitis media and subjects without otitis media. Method: This case-control study was done on 63 patients with chronic otitis media and 63 subjects without otitis media. Samples were taken from external ear canal with a sterile swab and were placed in the sterile tubes containing normal saline. The direct smear was prepared and samples were cultured in S, SCC and CMA (corn meal agar) media. Identification of genus and species were established using slide culture method and Germ-tube assay. Results: Positive fungi culture in external ear canal was seen in 77.78% and 17.46% of case and control groups, respectively (P<0.05). The most common type of fungi was Saprophyts (57.33%) followed by yeast (20.59%) and dermatophytes (17.32%). The most prevalent fungi in the subgroup of Saprophyts and dermatophytes was Aspergillusnigra (41.66%) and Trichophytonmentagrophytes (36.37%), respectively. The most common subgroup in yeast was Candida SPP with 53.85%. Conclusion: This study showed that the positive fungi culture in external ear canal is more prevalent in patients with chronic otitis media.
Gharaei A, Erahimzadeh A, Salimi Khorashad Ar, Jorjani O, Jamshidi A, Shah Bakhsh A, Noornia T,
Volume 17, Issue 2 (7-2015)
Abstract
Background and Objective: Vulvovaginal candidiasis or Candida vaginitis is a common fungal infection among adult women during reproductive ages and it is the second most frequent infection of the female genital tract. This study was carried out to determine of the prevalancy and species of vulvovaginal candidiasis and related clinical findings. Methods: This descriptive - analytical study was carried out on 400 women whom reffered to gynological center in Chabahar city in South-East of Iran during 2013. Vaginal samples were examined with direct microscopy and cultured on sabouraud dextrose agar and corn meal agar. Germ tube test and sugar assimilation test were carried out to differentiate the Candida species. The clinical findings including burning and itching during intercourse, burning with urination, foul-smelling vaginal discharge, vaginal deliveries, pH of the vagina, cervix appearance, consistency and color of vaginal in patients with vaginal discharge were recorded. Results: The prevalence of vulvovaginal candidiasis was 45.8%. Isolated candida species were candida albicans with 80.87% and Candida non-albicans with 19.13%. There was significant correlation between itching (85.52%) and burning during intercourse (67.87%), hypogastric pain (80.53%) with vulvovaginal candidiasis (P<0.05). 73 patients (39.9%) reported urinary burning. The most common affected by vulvovaginal candidiasis was with marriages age of 11-20 years old (P<0.05). Conclusion: The most common isolated species was candida albicans. The common clinical findings were burning, itching, smelly discharge, vaginal parity.
M Razmjoo , P Khaki , V Fadaee Noughani ,
Volume 18, Issue 3 (10-2016)
Abstract
Background and Objective: The adoption of methods for increasing the shelf life of dairy products by using natural preservatives is necessary. This study was done to determine the antimicrobial activity of aqueous extract of orange peel and its effect on the shelf life of flavored milks.
Methods: In this descriptive –analytical studty the antimicrobial activity of aqueous extract of orange peel was investigated by using disk diffusion method and minimum inhibitory concentration (MIC) by successive dilution of culture broth and then its impact on the shelf life of milk.
Results: In disk diffusion method and MIC the antimicrobial effect of aqueous extract of orange peel was more effective against Staphylococcus aureus and Candida albicans and less effective on Escherichia coli. The growth diameter of disk diffusion method in aqueous extract of orange peel was 7.11, 29.06 and 50 mm for Staphylococcus aureus, Escherichia coli and Candida albicans, respectively. The inhibitory concentration in the aqueous extract of orange peel was 15, 2 and 2 mg/ml, respectively. Also 0.17 g/ml of aqueous extract of orange peel in milk reduced the growth of microorganisms at the time of 6, 12, 24, 48 and 72 hours. Temperature affected the growth of Candida albicans in the milk, so that the growth of microorganisms reduced with decreasing temperature (P<0.05). The growth inhibitory activity of the aqueous extract of orange peel on Staphylococcus aureus was significantly more than on Escherichia coli (P<0.05).
Conclusion: This study showed that the antimicrobial activity of aqueous extract of orange peel on Staphylococcus aureus, Escherichia coli and Candida albicans in vitro and in the milk.
A Kazemi , H Nowrozi , M Badiee Moghadam ,
Volume 19, Issue 2 (7-2017)
Abstract
Background and Objective: Candida albicans is the normal flora of the body as opportunistic fungi. It causes candidiasis in immunocompromised condition. This study was done to drug susceptibility testing of Candida albicans isolated from patients against Amphotericin B and Ketoconazole.
Methods: In this descriptive – analytic study, drug susceptibility of 30 Candida albicans isolated from patients admitted to Tehran hospitals, Iran was tested against Amphotericin B and Ketoconazole by micro dilution method in accordance with CLSI M27-A2 guideline and disk diffusion method in accordance with CLSI M44-S2 guideline. Standard isolate Candida albicans PTCC (5027) and Candida krusei PTCC (5295) were used for quality control.
Results: The minimum and maximum MIC against Amphotrericin B was 0.0625 µg.ml-1 and
4 µg.ml-1, respectively. The minimum and maximum MIC against Ketoconazole was 0.5 µg/ml-1 and 32 µg/ml-1, respectively. The minimum and maximum zone diameter was 6 and 28 mm for both drugs. The results of drug susceptibility testing by two methods did not show significant differences. 25 isolates (83.3%) against ketoconazole and 2 isolates (6.7%) against Amphotericin B were resistant.
Conclusion: Amphotericin B administration seems better choice in candidiasis treatment in comparision with Ketoconazole.
Sousan Mohammadi , Fatemeh Keshavarzi , Behnaz Manouchehri , Fariba Lahoorpour , Mohammad Raza Javaheri ,
Volume 22, Issue 2 (6-2020)
Abstract
Background and Objective: Candida species are the second most common cause of vaginitis in worldwide after bacterial agents. This study was performed to determine the etiological factors of Candida vulvovaginitis in pregnant women.
Methods: This descriptive-analytical study, was performed on 100 pregnant women with vaginal discomfort that referring to gynecologic and obstetric clinics of the Sanandaj Social Security Hospital, Kurdistan province, Iran during 2016. After obtaining informed consent and collecting background information using a questionnaire, the patients were examined by a specialist physician and samples were collected. Identification of isolates was done based on colony color and also using PCR-restriction fragment length polymorphism (PCR-RFLP) method.
Results: Candida vulvovaginitis was seen in 29% of women. Candida albicans were the most common cause of the disease (86.2%) followed by Candida paraposilosis (10.43%) and Candida glabrat (45.3%) . There was no significant relationship between diabetes and candida vulvovaginitis. There was a significant relationship between the history of antibiotic usage, age over 35 and third trimester of pregnancy with Candida vulvaginitis (P<0.05).
Conclusion: The prevalence of Candida vulvovaginal candidiasis was 29% and Candida Albicans was the most common cause of the disease.
|
|
