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Breast cancer is one of the most common causes of death due to cancer in women. More than half of hereditary breast/ovarian cancer families could be attributed to mutation in breast cancer susceptibility gene BRCA1. This study was performed on blood samples of 30 women who affected with familial breast cancer. Non-radioactive PCR-SSCP technique was utilized mutation screening in exons 3, 10, 12 of BRCA1 gene. Two shifts in exon 3 and also two in exon 12 was detected, but no shift in exon 10 was found. Due to low number of recognized mutations, the statistical analysis didn’t show a meaningful correlation between mutations and pathological characteristics. Results from this study showed that there was a low possibility of germline mutation in these three exons. Low rate of mutation in this report was concordance with the others.

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Background and Objective: Breast cancer is one of the most frequent malignancies among women. This study was done to determine the BRCA1 gene expression in 7,12-Dimethylbenz(a)anthracene (DMBA) induced breast cancer in rats. Materials and Methods: In this experimental study, the breast cancer was induced by DMBA in Sprague dawley rats. After tumors arise, cell cultures were prepared and G-banding staining was performed on metaphase chromosomal smear. According to databases, genes in the affected area were collected and after comparing genome of the rats and human in changed chromosomal segments, a gene list was prepared. FISH technique was performed on BRCA1 gene to prove accuracy of chromosomal banding results. Results: Structural changes such as deletion occurred in chromosomes 10, which BRCA1 is located on. 24.7% of cells showed evidence of physical deletion in both copy of BRCA1 gene and 23.8% of cells showed deletion in one copy. Conclusion: Induced DMBA Breast cancer cells showed deletion in BRCA1 copy numbers. This gene may be involved in animal breast tumor model.