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Showing 18 results for Antibiotic

H. Nooralhhi (m.d), M .rostami (m.d),
Volume 2, Issue 1 (3-2000)
Abstract

The assessment to bacterial epidemiology in large hospitals help the management of the febrile patients and prescribing the correct kind of antibiotics, before receiving the laboratory results. In a descriptive study during (1996-97) in different hospital departments. The samples were transferred to the laboratory and common bacterias were isolated. In this study, cultural mediums of stool, blood, wound, were used as source the samples the results indicate that, the most common bacteria was E.coli with 30.6%, Staphylococci 14.2% and common infection sites were, urinary 33.3%, blood 21.7% respiratory tracts 14% respectively. The results from this investigation shows that frequency and distribution pattern agrees with those reported by other researchers. The susceptibility of isolated bacteria to Ciprofloxaxin and amino glycosides were quiet high.
Ghazi-Moghaddam B, Tajari Hr, Rabiee Mr, Balmeh M, Kamangari A,
Volume 8, Issue 3 (10-2006)
Abstract

Background&Objective: Transurethral resection of prostate (TUR-P) is the Gold standard therapy in benign prostatic hypertrophy (BPH). Urinary tract infections are one of the most important complications of this method. Prophylactic antibiotic regimen and the duration in preventing the infections are controversial. This study was designed to compare three different prophylactic regimens in TUR-P. Materials&Methods: In this interventional study, patients with sterile urine analysis and culture prior to TUR-P were classified in to three groups (A, B and C). One single-dose Keflin was prescribed before the operation. Four additional doses of Keflin were given to group B patients after TUR-P. Group C was similar to group B but Ciprofloxacin was given to them until catheter removal. Data were analyzed with SPSS-11.5. ?2 test and ANOVA were used. P-value less than 0.05 considered significant. Results: The incidence of positive urine cultures in group A, B and C were 35%, 20% and 11.5%, respectively. No significant relationship was seen between UTI and antibiotic regiments. Conclusion: There was not any significant relationship between UTI and different regimens although UTI rate was lower in the group with longer duration of the prophylaxis. Complementary studies are suggested for application of this regimen.
Aniya Ahani-Azari, Ahmad Danesh,
Volume 9, Issue 3 (10-2007)
Abstract

Background & Objective: in recent years antibiotic resistance of Pseudomonas aeruginosa is increased as a causetive factor in hospital contamination. This study was done to determine the prevalence and antibiotics susceptibility of Pseudomonas aeruginosa isolated from inanimate objects in Taleghani hospital.

 

Materials & Methods: This descriptive study was done on inanimate objects in different wards of Taleghani hospital during 2006. Sampling was performed by sterile gauze pad for dry surfaces and swab for wet surfaces. To differentiate and identify P.aeruginosa, various culture media such as Brain Heart Infusion Agar, Citrimide Agar and Triple Sugar Iron Agar were used. Antibiotic sensitivity of the isolated strains was determined by Kirby-Bauer Method using Muller-hinton Agar medium. Moreover, antiseptic sensitivity of the strains to Decosept and Povidone-Iodine as the main antiseptic agents was identified by determining Minimal Bactericidal Concentration and Minimal Inhibitory Concentration using Nutrient Agar and Nutrient Broth media.

 

Results: Fifty-five of the total 292 samples were positive for P.aeruginosa. Based on the ratio of the number of positive samples to the total samples, Thalassemia ward (38.5%) was the most infected ward and taps (61.1%) was the most infected sampling place. None of the samples from room atmosphere and intensive care equipments were positive for P.aeruginosa. 20% of the samples were resistant to ceftazidime and 32.7% were resistant to piperacillin, also 10.9% of samples were resistance to both ceftazidime and piperacillin.

 

Conclusion: This study showed that contamination with P.aeruginosa and antibiotic resistance of the isolated bacteria are the main problems in Taleghani hospital.


Barati L (md), Ghezelsofla F (md), Azarhoush R (phd), Heidari F (bsc), Noora M (bsc),
Volume 13, Issue 3 (10-2011)
Abstract

Background and Objective: Urinary tract infection is the most common bacterial infection during pregnancy. The pregnant women seems to be at risk for pyelonephritis and untreated infection. Timely recognition and on-time appropriate treatment of urinary tract infection particularly in pregnant women reduce the related complications. This study was done to assesse Sensitivity of isolated E.coli from pregnant women urine to antibiotics. Materials and Methods: In this descriptive study E.coli isolated from 360 urine samples from pregnant women, were examined, using Eosin Methylene Blue, blood sugar method. Antibiogram diffusion disk Kirby-Bauer was performed to assess the antibiotic response. Results: The persent of sensitivity of Escherichia coli to antibiotics were Co-amoxiclav (5.72%), Ampicillin (8.86%), Amoxicillin (11.87%), Cefazolin (32.12), Cephalexin (36.1%), Gentamicin (40.28%), Co-trimoxazole (48.15%), Nalidixic acid (55.3%), Nitrofurantoin (72.48%) and Ceftriaxone (80.78%). Conclusion: This study showed that there is a high level of E.coli antibiotics resistance toward Amoxicillin and Ampicillin high sensevity is related to Ceftriaxone and Nitrofurantoin in this region.
Hashemi Sr (phd), Davoodi H (phd),
Volume 13, Issue 4 (12-2011)
Abstract

Antibiotics usage in domestic animals, as antibiotic growth promoters (AGPs) was considered from middle of 1950 onwards. Antibiotic feed additives as growth promoters have long been supplemented to animal food in very low amounts (5 to 20 ppm) to stabilize the intestinal microbial flora, improve the general performances, and prevent some specific intestinal pathogens. The widespread use of antibiotics as AGPs is playing a significant role in the emergence of resistant bacteria. The resistant bacteria in animals due to antibiotic exposure can be transmitted to human through the consumption of their products, from close or direct contact with animals, or through the environment and consequently, the increased concern about the potential for antibiotic resistant bacteria was led the European ::::union:::: and the United States regulations banning the use of AGPs in animal food since 2006. Various studies always look for a suitable replacement antibiotics to substitute to preserve the valuable role of these compounds. Extensive efforts on these alternatives have been started in last decades and some of these alternatives such as probiotics, prebiotics, synbiotics, ionophores, acidifier, organic acids, medicinal herbs and phytogenic can be pointed out. Hence, the aim of this review is to evaluate the antibiotic replacement strategy particularly medicinal herbs and their mechanisms as new growth and health promoters.
Hashemi A (msc), Shams S (msc), Kalantar D (msc), Taherpour A (msc), Barati M (msc),
Volume 14, Issue 1 (3-2012)
Abstract

Background and Objective: Pseudomonas aeruginosa is one of the important causes of nosocomial infections. Extended spectrum-beta Lactamases (ESBLs) and Metallo-beta Lactamase (MBL) producing strains have become resistant against a wide range of antibiotics. The aim of this study was to determine the effect of Methanol extract of Camellia Sinensis on Pseudomonas aeruginosa producing ESBL isolated from burnt wounds of patients.

Materials and Methods: This descriptive study was done on burnt wounds of 245 hospitalaized patients in Shafa hospital, Kerman, Iran during 2006-07. ESBLs producing strains were detected by phenotypic confirmatory test and also E-test strips were used for MBL detection. P.aeruginosa MIC was determined for Cefotaxime, Ceftazidime, Azteronam, Imipenem, Meropenem and methanol extracts of plant Camellia Sinensis prepared by Maceration method.

Results: 120 of burnt wound infected with P.aeruginosa, out of them 41 isolates contained ESBL while lacked MBL. 60% of isolates were resistant to Cefotaxime, 66% to Ceftazidime, 42% to Azteronam, 3% to Imipenem and 5% to Meropenem. Among the extracts, green Tea had the highest antibacterial effect on standard strains and P.aeruginosa producing ESBLs in 1.25mg/ml concentration.

Conclusion: This study showed that methanolic extract of green tea has higher antibacterial effect aginst β-lactamase P.aeruginosa strains than Cefotaxime and Ceftazidime.


Choopani A, Golmohmmadi R, Rafati H, Imani Fooladi Aa,
Volume 14, Issue 3 (10-2012)
Abstract

Background and Objective: Considering the significant incidence of nosocomial infections in hospitalized patients, this study was done to determine the prevalence of Staphylococcus aureus strains isolated from wound infection and drug sensitivity pattern, Tehran-Iran. Materials and Methods: In this descriptive study, Staphylococcus aureus isolated and identified according to standard procedures from the wound infections of 614 patients referred to Baqiyatallah hospital, Tehran-Iran during 2006-07. The samples were examined and antibiogram was performed by disc diffusion method on Mueller Hinton agar with 12 antibiotics. Results: 100 (16.28%) of wound infection of Staphylococcus aureus was isolated from 614 patients. The infection rate in men was twice compared to women. The highest rate 29 (29%) was observed in people aged 40 to 60 group. Also specimen's patients with immunosuppressive diseases (28 cases), surgical site infection (16 cases) and normal wounds (13 cases) were considered to be most prevalent isolates. Antibiotic sensitivity testing revealed that 96 (96%) of isolates were sensitive to vancomycin, 95 (95%) and 92 (92%) were resistant to penicillin and cotrimoxazole, respectively. Our result showed that 43% of strains were resistant at 11 antibiotics. Conclusion: This study showed that the prevalence of Staphylococcus aureus was 16.28% of samples, with 43% antibiotic resistance. The highest sensitivity was toward to vancomycin.
Alboghobeysh H, Tahmourespour A , Doudi M,
Volume 15, Issue 1 (3-2013)
Abstract

Background and Objective: Pollution due to the heavy metals is a problem that may have negative consequences on the hydrosphere. Identification of microorganisms resistant to heavy metals plays an important role in relation to environmental pollution bioremediation. This study was done to assess the antibiotic resistance in isolated bacteria from urban sewage and copper smeltery industrial wastewater. Materials and Methods: This laboratory study was done on the wastewater samples from urban sewage and copper smeltery in Isfahan-Iran, during 2011-12. Heavy metal resistant microorganisms were isolated and enumerated after serial dilution and culturing on PHG agar plates supplemented with 0.5 mM of each heavy metal. The pattern of resistance was assigned by Minimum Inhibitory Concentration (MIC). Antibiotic resistance toward following medicines Ofloxacine, Penicilline, Sulfometoxasole, Lincomycin, Kanamycin, Streptomycin, Clindamycin, Vancomycin, Cefradin and Neomycin were subsequently investigated. Results: The greatest resistance in isolated bacteria has been related to the Ni (MIC: 24 mM) that’s related to the genus of Klebsiella and its minimum MIC is 2mM that’s of Acinetobacter lwoffi, Providencia stuartii, Branhamella. The minimum degree of resistance is related to Cu. Its Maximum MIC related to this metal in swage is 2 mM that’s of Klebsiella pneumoniae and its minimum degree of resistance in copper smeltery effluent is arranged as 1mM related to genus of Pseudomonas alkaligenes. In examining multi metal resistance pattern, the greatest resistance with 8 mM MIC has been related to the Cd, that’s of Ni resistant Moraxella osloensis in Copper smeltery effluent. The highest portion of isolated bacteria were resistant to Pb (P<0.05). The highest resistance of refirary wastewater was to Ni (MIC: 2 mM), which belong to Klebsiella (P<0.05). The lowest belong to Acine to bacter Lwoffi providencia Stuartii, Branhamella (MIC: 2mM) (P<0.05). In urban sewage: the highest resistance belong to copper, which was seen in Klebsiella Pneumoniae (MIC: 2mM). In Antibiogram tests of Klebsiella, Moraxella and Escherichia, it was demonstrated that the metal resistant bacteria were also resistant toward Lincomycin, Kanamycin, Streptomycin, Clindamycin, Vancomycin, Cefradin and Neomycin, as well. Conclusion: Increasing heavy metal resistance in the environment leads to increased antibiotic resistance toward microorganisms.
Soltan Dallal Mm , Rastegar Lari A, Sharifi Yazdi Mk,
Volume 16, Issue 1 (3-2014)
Abstract

Background and Objective: Gastroenteritis due to Salmonella is common in human and considered as a global dilemma of public health. This study was done to determine the Pattern of serotyping and antibiotic resistance of Salmonella in children with diarrhea in Iran. Methods: In this laboratory study, 306 stool samples were collected from children with diarrhea in public health centers in Robat-karim, Tehran province, Iran. The specimens were enriched in Selenite F medium and then cultured on Hekton agar. The identification of Salmonella was carried out by conventional method and antimicrobial susceptibility testing was performed according to CLSI procedures. Results: Out of 306 stool samples, 7.2 % were identified as Salmonella species, as follow: 7 Salmonella typhi, 6 Salmonella paratyphi B, 3 Salmonella paratyphi C, 2 Salmonella paratyphi A and 4 samples were not identifiable. There was a significant relation between presence of WBC in fecal and salmonellosis (P<0.05). In drug sensitivity trends, 92.3% of Salmonella species were sensitive to chloramphenicol, ceftizoxime, Nalidixic acid and Amikacin. Conclusion: This study showed that Salmonella was the cause of children diarrhea in 7.2% in this region.
Alavi Sm , Roozbeh F , Behmanesh F,
Volume 16, Issue 2 (7-2014)
Abstract

Background and Objective: Indiscriminate use of antibiotics is one of the major health problems worldwide. Rapidly increasing incidence of antibiotic-resistant microbes warns healthcare authorities in the country to use drugs, reasonably. This study was carried out to assess the pattern of antibiotic usage in Razi teaching hospital in Ahvaz, southwest of Iran. Methods: This descriptive –analytical study was conducted on 17668 hospitalized patients in Razi teaching hospital Ahvaz, southwest of Iran during 2012. Proper or inappropriate antibiotic usage was judged based on existing national guidelines or guidelines referenced in textbooks. Results: 3119 of patients were received antibiotics which 20.4% of patients were being treated unnecessarily. 2482 (79.6%) of patients had indications for antibiotic therapy. Out of 2482 patients with indications for antibiotic therapy, 51.9%, 18.1 % and 6.5 % of patients were received incorrect antibiotic regiment, inadequate dose and unsuitable duration of therapy, respectively. The highest rate of unnecessary, inappropriate, incorrect duration and dosage of antibiotics were observed in internal, scorpion wards and the lowest was in infectious diseases and obstetrics wards (P<0.05). Conclusion: The pattern of antibiotic usage in Razi teaching hospital in Ahvaz was inappropriate. Unnecessary prescription, inappropriate antibiotic spectrum, inadequate dose and duration of antibiotics were inappropriate treatment strategy.
Ahmadi Jalali Moghadam M , Honarmand Hr, Falah-Delavar S, Saeidinia A,
Volume 16, Issue 3 (10-2014)
Abstract

Background and Objective: Ruta graveolens is a plant used in traditional medicine with variety of therapeutic effects. This study was done to evaluate the antimicrobial effect of hydro and alcoholic extracts of Ruta graveolens on 8 species of pathogenic bacteria. Methods: In this descriptive study, standard strains of Enterococcus faecalis, Staphilococcus aureus, Staphilococcus epidermis, Strptococcus pneumonia, Escherichia Coli, Klebsiella pneumonia, Salmonella typhi and Enterobacter Cloace were obtained from Iranian institute of scientific and industerial research center. The effect of hydro and hydroalcholic extracts of Ruta graveolens on the growth of bacteria was determined by disc diffusion and serial macro dilution methods. Minimum inhibitory concentration (MIC) for each extract was determined and the findings were compared with 8 common antibiotics including Co-trimoxazole, Oxycycline, Vancomycin, Erythromycin, Furazolidon, Tetracycline, amoxicillin / clavulanic acid and Ampicillin/sulbactam. Results: Hydro and hydroalcoholic extracts of Ruta graveolens did not show any inhibitory effect on growth of the pathogenic bacteria even in the presence of high concentration (3mg/ml). Conclusion: The extracts of arial organs of Ruta graveolens had no inhibitory effect on growth of the common human pathogenic bacteria.
Soltan Dallal Mm , Vafaei Z, Haghi Ashtiani Mt , Nikmanesh B , Rahimi Foroushani A,
Volume 17, Issue 1 (3-2015)
Abstract

Background and Objective: Yersinia enterocolitica is a worldwide pathogen belong to genus Yersinia. The association between acute childhood diarrhea and other diseases caused by Yersinia enterocolitica has been established by several researchers. Due to the lack of sufficient information on other pathogenic Yersinia species, this study was done to determine the prevalence and the pattern of antibiotic susceptibility of atypical Yersinia spp isolated from children, less than 14 years old. Methods: This descriptive cross - sectional study was carried on 384 children with diarrhea whom referred to the Tehran children medical center, Tehran, Iran during August 2011 to August 2012. 384 fecal specimens of children were transferd to the laboratory and cold enrichment in alkaline buffer with pH of 7.2 for 21 days. The samples were cultured in Cefsulodin-Irgasan-Novobiocin Agar (CIN) differential media in 7, 14, and 21 days. The identification of Yersinia species were carried out by conventional procedure. Antibiotic susceptibility test to Ciprofloxacin, Co-trimoxazole, Tetracycline, Chloramphenicol, Erythromycin, Ampicillin, Gentamicin, Penicillin were determined by standard disk diffusion method. Results: Out of 384 fecal samples, 3 (0.7%) were infected with Yersinia. Three species of Yersinia were Y.enterocolitica, Y.Kristensenii and Y.frederiksenii. All three strains were sensitive to Cotrimoxazole, Tetracycline, Chloramphenicol, Ciprofloxacin and Gentamicin. Conclusion: This study showed that atypical Yersinia play important role in diarrhea. Therefore, more attention should be noticed to atypical Yersinia species in addition to Yersinia enterocolitica.
Kavoosinezhad F, Fattahi E, Moori Bakhtiari N ,
Volume 18, Issue 2 (6-2016)
Abstract

Background and Objective: Resistance of Staphylococcus aureus to antibiotics is one of the major global health problems in human societies. Thus, evaluation of pattern of antibiotic resistance in its different strains is very important. This study was carried out to evaluate the antibiotic resistance in Staphylococcus aureus isolated from clinical samples by disk diffusion and PCR methods.

Methods: In this laboratory- descriptive study, 50 isolates of Staphylococcus aureus to be identified from clinical specimens. Methicillin resistance was examined using PCR and antibiotic susceptibility of isolates was tested by disk diffusion method.

Results: 50 isolates were resistant to methicillin, ampicillin and penicillin. The resistance of isolates to erythromycin, Gentamicin, Clindamycin and Ciprofloxacin were 48%, 34%, 34%, 34%, respectively. The PCR method showed that 98% of Methicillin Resistance of Staphylococcus aureus isolates carried the methicillin resistant gene.

Conclusion: This study indicated that 98% isolates harbor mecA genes and more resistant to methicillin related mecA genes.


C Halakoo , J Nowroozi , P Pakzad , H Koohsari , M Seyyed ,
Volume 18, Issue 4 (12-2016)
Abstract

Background and Objective: Water resident bacteria are potentially important reservoir of antibiotic resistance genes. This study was performed to evaluate the antibiotic resistance in Escherichia coli strains isolated from raw waters of Golestan province, northern Iran.

Methods: In this descriptive study, 26 samples from Ziarat river water (13 samples pre & 13 samples post treatment) and 36 samples from Azadshahr area springs water (18 samples pre & 18 samples post treatment) were collected. 75 numbers of Escherichia coli bacterium samples (50 isolated from river and 25 isolated from springs) identified and isolated from raw waters of Golestan province, northern Iran by MPN method via differential tests. Susceptibility of Escherichia coli strains to 11 antibiotics (Amoxicillin / Clavulanic acid, Ampicillin, Imipenem, Cefalotin, Cefotaxime, Gentamicin, Amikacin, Tetracycline, Nalidixic acid, Ciprofloxacin and Trimethoprim / Sulfamethoxazole) was assayed by disk diffusion Kirby & Bauer’s method.

Results: 14 spring's raw water samples and 12 river raw water samples contained Escherichia coli. All of the river and springs samples assayed free from Escherichia coli post treatment. All of the Escherichia coli strains isolated from samples showed the similar phenotypical resistance against to surveyed 11 antibiotics. The most significance resistance to Ampicillin (river 94% & springs 88%), Amoxicillin / Clavulanic acid (river 76% & springs 80%), Tetracycline (river 14% & springs 16%) and Cefalotin (river 8% & springs 16%) viewed. Resistance to Trimethoprim / Sulfamethoxazole (8%), Nalidixic acid (2%) and Ciprofloxacin (2%) just viewed in river samples. All of the river and spring isolates were sensitive to Imipenem, Cefotaxime, Gentamicin and Amikacin and demonstrated intermediate resistance to others antibiotics.

Conclusion: Treatment of raw water from springs and rivers caused the eradication of Escherichia coli. As regard to observed phenotypical resistance in springs’ raw waters, presumably with lack of treatment springs’ raw water can be caused the transmission of antibiotic resistance to human body.


Sh Shahraki , M Rabi Nezhad Mousavi , B Dahmarde , M Atashgah ,
Volume 19, Issue 2 (7-2017)
Abstract

Background and Objective: Enterococci is gram positive bacteria which is the inhabitants of gastrointestinal tract. Hospital infections and antibiotic resistance to enterococci is increased. This study was done to determine the molecular evaluation of vanA and vanB genes of enterococci isolates resistant to Vancomycin and Teicoplanin.

Methods: In this descriptive study, 113 isolates samples were collected and identified according to biochemical test and cultural characteristics in Ali ibn Abi Talib hospital in Zahedan, Iran. Antibiogram test was done to determine antibiotic resistance pattern. E-test strip was used to evaluate the minimum inhibitory of concentration (MIC). PCR was used to detect the vanA and vanB genotype in Vancomycin and Teicoplanin resistance enterococci.

Results: 92%, 6.2% and 1.8% of isolated samles collocted from urine, blood culture and pleura fluid, respectively. According to phenotype, 18.6% and 17.69% were resistance to Vancomycin and Teicoplanin, respectively. Resistance was observed in strains of Enterococcus faecalis and Enterococcus faecium. VanA genotype was seen in all of the resistance isolated species.

Conclusion: This study showed that strains of Enterococcus faecalis and Enterococcus faecium have more antibiotic resistance to the Vancomycin and Teicoplanin, morever vanA genotype precence in all of resistance isolated samples.


A Monazami , F Haghi ,
Volume 19, Issue 2 (7-2017)
Abstract

Background and Objective: Pseudomonas aeruginosa is an opportunistic pathogen with numerous virulence factors such as phospholipase and type IV pili. The emergence of multidrug resistant Pseudomonas aeruginosa has become a serious public health threat worldwide. This study was done to determine the frequency of plcH, plcN, pilA and pilB genes in multi-drug resistant Pseudomonas aeruginosa isolated from clinical samples.

Methods: In this cross-sectional study, 93 isolates of Pseudomonas aeruginosa collected from different clinical samples from hospitals of Zanjan, Iran during 2013-14. After identification of isolates by biochemical tests, antibiotic susceptibility testing (Kirby-Bauer) was performed according to CLSI guidelines. Total DNA extracted and PCR was done to detect of plcH, plcN, pilA and pilB genes.

Results: Among 93 of Pseudomonas aeruginosa isolates, the highest antibiotic resistance related to Erythromycin and Cefoxitin (95.6%) and the lowest resistance related to Amikacin (26.8%). 80.6% of isolates were multidrug resistant (MDR). Out of 75 MDR isolates, the frequency of plcH, plcN, pilA and pilB genes was 97.4%, 49.3%, 26.6% and 17.3%, respectively.

Conclusion: According to high frequency of phospholipase C gene (plcH) in MDR Pseudomonas aeruginosa isolates which isolated from different clinical samples, presumably this virulence factor plays an important role in pathogenesis of this bacterium.


Aida Babazadeh Naseri , Mohammad Mehdi Soltan Dallal,
Volume 21, Issue 1 (3-2019)
Abstract

Background and Objective: Listeria monocytogenes is an important food-borne intracellular pathogen which can transmit to human through contaminated foods and causing meningitis, meningoencephalitis and abortion. This study was done to determine the frequency, antimicrobial susceptibility and serotyping of Listeria monocytogenes isolated from food samples in Tehran, Iran.
Methods: This descriptive was carried on 150 food samples including vegetables, cheese and meat were collected from supermarkets, open-air markets, and delicatessens in different regions of Tehran, Iran since April to September 2018. The presumptive isolates were characterized biochemically. All L. monocytogenes isolates were further analyzed by serotyping and antimicrobial susceptibility tests.
Results: Out of 150 samples, Listeria spp. was detected in 30 (20%) samples in which 9 (6%) were positive for L. monocytogenes [vegetables (n=4, 44.44%), cheese (n=2, 22.22%) and meat (n=3, 33.33%)]. of the 9 L. monocytogenes isolates, 5 (55.55 %), 3 (33.33 %), and 1 (11.11%) belonged to serotypes 4b, 1/2b, and 1/2a, respectively. The most L. monocytogenes isolates were resistant to Trimetoprime, Sulfamethoxazole, Tetracycline, Streptomycin, Chloramphenicol, and Ciprofloxacin while were sensitive to Penicillin G, Gentamicin, Streptomycin, and Ampicillin, and were intermediately resistant to Ciprofloxacin.
Conclusion: The rate of Contamination of vegetable, cheese and meat samples with L. monocytogenes is important in Tehran, Iran. Due to the potential contamination samples to Listeria, there is necessity need for continuous monitoring and the development of a precise program for identifying this bacterium in Tehran and the whole country.

 
Abolfazl Sirdani , Zahra Rajabi , Fatemeh Fardsanei , Saeid Vahedi , Mohammad Mehdi Soltan Dallal ,
Volume 21, Issue 4 (12-2019)
Abstract

Background and Objective: Salmonellosis is a gastroentritidis which caused by the different serovars of Salmonella genus, and responsible for morbidity and mortality worldwide. Food born disease is one of the growing problems of human societies especially in developing countries. The aim of this study was to evaluate and serogroup determination of Salmonella isolates from food along with antibiotic resistance pattern.
Methods: This descriptive study was performed on total of 400 in equal of 200 packed and 200 unpacked  samples of (red meat, chicken meat, egg, vegetable) collected in random from distributed in Tehran ,Iran during nine months in 2018. Microbial, biochemical and serological test was performed according to protocol number of 1800 of national standard. Antimicrobial susceptibility test was done by disk diffusion (MAST, Co, UK) method.
Results: Out of 400 samples 8 (2%) was identified as Salmonella. The unpacked foods were more contaminated (75%) compared to packed foods (25%). The most isolated serogrouping were belonging to especially D. Salmonella. The chicken samples were more contaminated (37.5%) than other samples. The isolated Salmonella were mostly resistance to nalidixic acid (75%).
Conclusion: The Salmonella isolated particularly from group 1 showed higher antimicrobial resistance, additional care should be taken in preparation, packaging and supplying the food samples.


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مجله دانشگاه علوم پزشکی گرگان Journal of Gorgan University of Medical Sciences
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