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Showing 2 results for Wistar Rats
Laleh Mavaddatiyan , Shiva Khezri , Seyyed Meysam Abtahi Froushani ,
Volume 24, Issue 2 (7-2022)
Abstract
Background and Objective: Multiple sclerosis (MS) is a common neurological disease that increases oxidative stress and causes immune system disorders. Curcumin is the active component of turmeric with anti-inflammatory properties. This study was conducted to determine the effects of curcumin on cortisol, catalase, and nerve growth factor (NGF) expression in an animal model of MS.
Methods: This experimental study was conducted on 30 female Wistar rats. Experimental autoimmune encephalomyelitis (EAE) was chosen as an experimental model of MS. The rats were divided into 3 groups of 10, including a healthy control group, an affected group, and a group treated with curcumin. The disease was induced by immunization of rats with homogenized guinea pig spinal cord and Freund's complete adjuvant. Then, the immunized animals were allocated into two equal groups. Treatment with curcumin (100 mg/kg daily) was started 12 days after the immunization when the rats showed the first symptoms of neurologic disability. The treatment was continued until day 24 post-immunization. Simultaneously, the EAE group received the medicine solvent (distilled water). Finally, the rats' weights as well as cortisol, catalase, and NGF levels were measured in the study groups.
Results: Curcumin significantly increased the level of cortisol to a level equal to that of healthy rats (P<0.05). It also significantly increased the expression of NGF and reduced the amount of catalase in the affected rats (P<0.05). The curcumin administration significantly increased the overall weight of rats with MS but had no significant effect on the spleen weight of the treated rats.
Conclusion: Curcumin can be beneficial for treating EAE by reducing the destructive effects of oxidative damage and increasing NGF.
Nasrin Alborzian Juneqani, Mohammad Fathi , Rahim Mirnasouri ,
Volume 25, Issue 1 (3-2023)
Abstract
Background and Objective: Understanding the cellular signaling mechanisms involved in muscle hypertrophy is considered a scientific challenge. Mammalian target of rapamycin (mTOR) is one of the regulatory factors in this process that increases protein synthesis in skeletal muscle through phosphorylation. This study aimed to determine the effect of six weeks of high-intensity interval training (HIIT) on phosphorylated mTOR protein in the quadriceps muscles of adult male Wistar rats.
Methods: In this experimental study, 16 adult male Wistar rats (six weeks old and weighing an average of 190.93±4.97g) were used. The animals were randomly divided into two groups of control and training (n=8). The training group underwent six weeks of HIIT on a treadmill, with five sessions per week. The load was increased during the six weeks from repeating the interval of 30 meters per minute for 30 seconds in the first sessions to eleven repetitions of the interval of 35 meters per minute for 30 seconds at the end of the sixth week, with rest intervals between the intervals at a speed of 13 meters per minute for 60 seconds. The control group did not undergo any training. The mice were anesthetized, and the Vastus lateralis of the quadriceps muscle was extracted. The level of phosphorylated mTOR protein in the quadriceps muscle was measured using the immunohistochemical method.
Results: HIIT significantly increased the levels of mTOR phosphorylation protein in male Wistar quadriceps femoris muscle compared to the control group (P<0.05).
Conclusion: Interval activity can have a positive effect on muscle hypertrophy through mTOR.
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