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Anemia especially those due to Iron deficiency is one of the nutritional problems. Women are more susceptible to anemia during the age of fertility. The main purpose of this study is to determine the prevalence of anemia among 18-35 years old women in the villages around Gorgan. It was carried out in the 20 villages on 415 women. They were chosen by compound sampling. The mean age of the cases was 24.92 years old. Blood sampling and filling the questionnaire were carried out. On the chosen samples ferum Iron, TIBC, was determined using spectrophotometer hematolog tests was carried out using electronic cell counter. The prevalence of Iron deficiency, anemia, and the anemia due to Iron depletion were 37.78%, 25.1% and 13.45% respectively. There was not a meaningful relationship between marital status age with Iron depletion anemia and anemia due to Iron depletion. The conclusion out of this research showed that the anemia and especially anemia due to Iron depletion is one of the main nutritional problems among the women living in the villages around Gorgan.

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Background and Objective: Irritable bowel syndrome (IBS) is one of the most common gastrointestinal disorders characterized by abdominal pain or discomfort and altered bowel habits. Although IBS etiology is poorly understood, some psychological disturbances and food allergies have been proposed. This study was done to determine the effect of food allergen exculsion on symptoms and quality of life in IBS. Materials and Methods: In this descriptive and anolytical study was done on 100 IBS patients in Tabriz, Iran during 2011. Food allergies of specific origin and effects of non allergic regimens on quality of life, pain severity and disease symptoms were evaluted during one month period. Data were analyzed using SPSS-16, student’s t-test, Chi-Square and Fisher’s exact tests. Results: Patients age were 31.5±7.2 between 15-43 years. Specific antibodies against allergic foods were detectable in 31 (31%) cases. Penaut showed to induce the highest food allergy in 5 (16.1%) patients. The median disease duration was 4.5±2.6 years in allergic patients while 3.5±2.9 in non allergic group with no significant difference. The changes is quality of life was significant in non allergic regimens after one month in allergic group (P<0.05) while disease symptoms such as mucus defecation and flatulence had no significant changes. Conclusion: This study showed that one month non allergic regimens reduce abdominal pain and improve quality of life in IBS patients.

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Background and Objective: Individuals habit has been changed due to technology improvement as a result fast food consumption has dramatically increased in Iran. This study was done to determine the effective factors on Fast food consumption among high-school students based on planned behavior theory.

Methods: This descriptive-analytic, cross-sectional study was conducted on 500 high-school students in Aliabad city in Golestan province in north of Iran in one year study from October 2014-2015. A valid and reliable researcher-made and self-administered questionnaire based on TPB constructs (including attitude, subjective norms, perceived behavioral control and behavior intention) was utilized to collect the data.

Results: The frequency of fast food consumption was 4.01 per month and hamburger and pizza were the most prevalent fast foods. TPB explained fast food consumption behaviors with relatively high R2 around 0.65, effectively. Frequency of fast food consumption was significantly associated with behavioral intention and subjective norms (P<0.05).

Conclusion: As friends are the effective subjective norm for student fast food consumption therefore, they must specifically be considered in developing beneficial educational interventions.

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Background and Objective: Permanent first molars are susceptible to caries due to their particular anatomical form, early development and lack of knowledge of parents about their permanence. This study was conducted to determine the index of first permanent dental careis (DMF6) and some of its related factors in 12 year-old students.
Methods: This descriptive-analytical study was carried out on 400 students (200 females and 200 males) using combined method (stratified and cluster sampling) from public and non-governmental schools in Gorgan, northern Iran during 2015-16. First permanent molar examination was carried out. The parents' education level, frequency of toothbrushing and daily consumption of sugar-containing snack foods was recorded in a questionnaire. Each decayed, missing and filled first permanent molar tooth was given number one. These numbers were combined to compute the DMF6 index for each individual.
Results: The DMF6 index was zero in 19.5% and the DMF6 index was determined to be between
1 and 4 in 80.5% of the children. The mean DMF6 index was 2.23±0.07 and significantly higher in girls (2.39±0.10) than in boys (2.07±0.11) (P<0.05). The DMF6 index decreased with increasing parental education and the frequency of brushing and reducing consumption of sugary meals.
Conclusion: Dental caries prevalence among 12-years-old students in north of Iran is higher (2.23) than global standard until 2020 (lower than 1). Gender of child, parent’s education, frequency of toothbrushing and consumption of sugar-containing snack foods plays an important role in the rate of dental caries.

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Background and Objective: Listeria monocytogenes is an important food-borne intracellular pathogen which can transmit to human through contaminated foods and causing meningitis, meningoencephalitis and abortion. This study was done to determine the frequency, antimicrobial susceptibility and serotyping of Listeria monocytogenes isolated from food samples in Tehran, Iran.
Methods: This descriptive was carried on 150 food samples including vegetables, cheese and meat were collected from supermarkets, open-air markets, and delicatessens in different regions of Tehran, Iran since April to September 2018. The presumptive isolates were characterized biochemically. All L. monocytogenes isolates were further analyzed by serotyping and antimicrobial susceptibility tests.
Results: Out of 150 samples, Listeria spp. was detected in 30 (20%) samples in which 9 (6%) were positive for L. monocytogenes [vegetables (n=4, 44.44%), cheese (n=2, 22.22%) and meat (n=3, 33.33%)]. of the 9 L. monocytogenes isolates, 5 (55.55 %), 3 (33.33 %), and 1 (11.11%) belonged to serotypes 4b, 1/2b, and 1/2a, respectively. The most L. monocytogenes isolates were resistant to Trimetoprime, Sulfamethoxazole, Tetracycline, Streptomycin, Chloramphenicol, and Ciprofloxacin while were sensitive to Penicillin G, Gentamicin, Streptomycin, and Ampicillin, and were intermediately resistant to Ciprofloxacin.
Conclusion: The rate of Contamination of vegetable, cheese and meat samples with L. monocytogenes is important in Tehran, Iran. Due to the potential contamination samples to Listeria, there is necessity need for continuous monitoring and the development of a precise program for identifying this bacterium in Tehran and the whole country.

 

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Background and Objective: Salmonellosis is a gastroentritidis which caused by the different serovars of Salmonella genus, and responsible for morbidity and mortality worldwide. Food born disease is one of the growing problems of human societies especially in developing countries. The aim of this study was to evaluate and serogroup determination of Salmonella isolates from food along with antibiotic resistance pattern.
Methods: This descriptive study was performed on total of 400 in equal of 200 packed and 200 unpacked  samples of (red meat, chicken meat, egg, vegetable) collected in random from distributed in Tehran ,Iran during nine months in 2018. Microbial, biochemical and serological test was performed according to protocol number of 1800 of national standard. Antimicrobial susceptibility test was done by disk diffusion (MAST, Co, UK) method.
Results: Out of 400 samples 8 (2%) was identified as Salmonella. The unpacked foods were more contaminated (75%) compared to packed foods (25%). The most isolated serogrouping were belonging to especially D. Salmonella. The chicken samples were more contaminated (37.5%) than other samples. The isolated Salmonella were mostly resistance to nalidixic acid (75%).
Conclusion: The Salmonella isolated particularly from group 1 showed higher antimicrobial resistance, additional care should be taken in preparation, packaging and supplying the food samples.

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Background and Objective: The emergence of Gram-positive and Gram-negative bacteria resistant to antibiotics is a crisis worldwide. In this study, the antibacterial effect of zinc oxide nanoparticles was evaluated on standard and food isolated strains of Salmonella enteritidis and Bacillus cereus.
Methods: This descriptive laboratory study, zinc oxide nanoparticles were prepared on zeolite materials, and zinc level was determined using XRF. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of ZnO nanoparticles were determined using disc diffusion method.
Results: MIC value for all tested bacteria was 4 mg/ml and MBC values of standard and isolated strains of Salmonella enteritidis were 16 and 8 mg/ml, respectively, and for standard and isolated strains of Bacillus cereus was calculated in the range of 16 mg/ml.
Conclusion: Zinc oxide nanoparticles can inhibit Salmonella enteritidis and Bacillus cereus strains and may have a potential for its replacement with current preservatives to prevent food spoilage in industry.

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Background and Objective: The most common enterohemorrhagic Escherichia coli strain is the O157: H7 serotype, which is one of the most important intestinal pathogens and can cause complications such as hemorrhagic colitis, hemolytic uremic syndrome and acute renal failure. The aim of this study was to evaluate the enterohemorrhagic Escherichia coli causing molecular outbreaks of foodborne illness in Iran.
Methods: In this descriptive cross-sectional study, 189 fecal swab specimens were examined during April to September 2018. All suspected isolates were tested for biochemical tests. The isolates were confirmed by molecular PCR and evaluated by antimicrobial susceptibility tests.
Results: From 189 stool swab samples studied, 98 Escherichia coli isolates were detected based on phenotypic tests. Most of the outbreaks occurred in summer and the prevalence of enterohemorrhagic Escherichia coli was 24.5%, which 4% of them were non-O157H7. Most patients were between 1 and 12 years of age and the highest antibiotic resistance to cotrimoxazole and chloramphenicol was observed at 80% and 79%, respectively.
Conclusion: This study showed an increase in enterohemorrhagic Escherichia coli with 24.5% and an increase in antibiotic resistance to the antibiotics of chloramphenicol, cotrimoxazole and carbapenems. Increased resistance to imipenem and meropenem antibiotics makes it difficult to treat beta-lactamase-resistant strains.