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Background&Objective: Iron deficiency is the most common nutritional disorder in the world, The prevalence rate in developing countries is higher than developed countries, and also affecting a large number of child and women. The main purpose of this study was to promot the knowledge, attitude and practice of girl student in Ghaemshahr in order to prevent iron deficiency anemia. Materials&Methods: This study was an educational intervention to evaluate the effect of education on Knowledge, Attitude and Practice (K.A.P) in intervention and control groups (each group: 300) among female students in Ghaemshahr. The data was gathered using a questionnaire (include the demographic information, some of question related to knowledge, attitude, practice. K.A.P were assessed before and also 1.5 month after implementation of educational program using the questionnaire, collected data was analyzed by SPSS (11.5), STATA (8.0). Results: There was not significant difference between K.A.P in study group before implementation of educational intervention, but there is a significant increase between K.A.P after interventional education in intervention group. Educational intervention was promoted knowledge mounting to 15 score, 5 score to promote attitude and 1.6 score to improve the practice. There was significant relation between mother education, father job with their knowledge and class with their attitude, also mother education with their practice. Conclusion: The results indicated that improvement of knowledge could prevent iron deficiency anemia, thus emphasis on the health education in adolescent age is necessary to promote K.A.P.

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Background and Objective: The Problems caused by diabetes have direct and indirect impacts on quality of life of diabetic patients. By increasing of these problems quality of life of patients will decrease. This study was done to assess the effect of the educational program based on PRECEED (predisposing reinforcing enabeling causes educational diagnosis evaluation) model in promoting quality of life of patients with typeII diabetes.

Materials and Methods: This pre- and post-intervention quasi-experimental study was conducted on 78 patients with type 2 diabetes referred to diabetes clinic in Minoodasht located in Golestan province of Northern Iran. The educational program based on PRCEDE Model and patients educational needs was designed. The data were collected using two questionnaires. life-quality WHOQOL- BREF questionnaire and PRCEDE Model-based educational questionnaire. The subjects were followed for one month after intervention to identify the role of educational intervention on patients. The data was analyzed by using SPSS-16 and Paired t-test.

Results: After the intervention, the mean score of quality of life increased from 80.39±11.35 to 81.35±8.31. No significant difference was existed in the total score of quality of life after intervention but there were significant differences in the physical health (P<0.05), self-evaluation of quality of life of patients (P<0.01) and self-assessment of health of patients after educational intervention.

Conclusion: This study showed that the educational program based on PRECEDE model was effective in improving quality of life of patients with regard to physical health and self-assessment of their health status.

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Background and Objective: Several strains of the Echinococcus granulosus have been described based on morphological characters, intermediate host specificity and/or genetic analysis of mitochondrial and nuclear DNA. The aim of this study was to characterize different E.granulosus isolates by using sequences of mitochondrial atp6 gene.

Materials and Methods: In this study, Sixty infected liver and lungs of cattle, sheep and goats were collected from the abattoir of Varamin city-Iran during 2008. Protoscoleces were removed from each fertile cyst and DNA extracted. New and specific primers were designed for two existing genotypes (G1 and G6) of E. granulosus known to occur in Iran and applied in PCR reactions.

Results: The new primers selectively amplified the G1 and G6 genotypes of E. granulosus with specific bands of 708 and 705 bp respectively. The G1 genotype was identified in all fertile cyst samples.

Conclusion: This study showed that the new primer pairs which specifically amplify portions of the mitochondrial atp6 gene of the G1 and G6 strains of Echinococcus granulosus are proper molecular marker for investigating genetic variation in a number of isolates of E. granulosus from a range of hosts (sheep, goats, cattle) in Iran. The result of sequenced samples showed that our sequences were the same as those reported previously for these strains.

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Background and Objective: Parkinson disease (PD) is the second most common neurologic disorder that results following degeneration of dopaminergic neurons in the pars compacta of substintia nigra (SNc). The 1-methyl-1,2,3,6-tetrahydropiridine (MPTP) is a chemical neurotoxin that widely used in animal models of PD. This study was carried out to evaluate the numerical density of dark neurons (DNs) in the SNc in mice subjected to intraperitoneal and intranasal injection of different doses of MPTP. Methods: In this experimental study, 90 male adult BALB/c mice were randomly allocated into four experimental groups including: group 1 (MPTP was injected via i.p. at the dose of 20mg/kg per 2 hours for 4 times), group 2 (MPTP was injected via i.p. at the dose of 30mg/kg for 5 consecutive days), group 3 (MPTP was injected via i.n. at a single dose of 1mg/kg), group 4 (MPTP was injected via i.n. at a single dose of 1mg/kg), four sham and one normal groups. 20 days after the final injection, the animal's brain were removed and stained by toluidine blue. Numerical density of DNs was counted. Results: Intranasal injection of MPTP significantly increased density of dark neurons in the pars compacta of substintia nigra in compare to intraperitoneally injection of MPTP (P<0.05). Conclusion: Intranasal injection of MPTP is more effective manner to induce degeneration of neurons in substintia nigra in animal model of Parkinson's disease.

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Background and Objective: Diabetes meltius is a metabolic disorder which characterized with disorder in carbohytdrate and lipid metabolism. This study was conducted to evaluate the effect of aqueous extract of turnip root (Brassica rapa) on glucose and lipid Profile in alloxan-induced diabetic rats. Methods: In this experimental study 40 male wistar rats randomly allocated into 5 equal groups including diabetic control, Metformine 50mg/kg, 200mg/kg and 400 mg/kg/bw of aqueus extract of turnip root and normal control groups. Alloxan monohydrate 150 mg/kg/bw was used to induce diabetes mellitus and two weeks after Alloxan injection rats with fasting blood sugar (FBS) more than 350mg/dl considered as diabetic rats. All administrations were done orally and daily in a same volume for 28 consecutive days. The FBS concentrations were determined on the first, 14th and 29th days. On 29th day, blood was collected from overnight fasted rats. Plasma concentrations of total cholesterol (TC), triglyceride (TG), high density lipoprotein cholesterol (HDL-c), low density lipoprotein cholesterol (LDL-c), aspartate amino transfarase (AST) and alanine amino transferase (ALT) activities were measured. Results: The statistical data indicated (P<0.05) in the levels of FBS (4.5 times), TC, TG, AST and ALT (about 2.5 times) and LDL-c (2 times) significantly increased in diabetic rats compare to healthy normal control group. Administration of 200mg/kg and 400 mg/kg/bw of turnip root extract did not exhibit hypoglycemic activity. Turnip root extract significantly inhibited the increasing of TC, TG, LDL-c and ALT in diabetic rats (P<0.05), but had no effect on AST sera level. Conclusion: Although, the aqueous extract of turnip root had not any hypoglycemic activity but it was effective in reduction of TC, TG, LDL-c and ALT in diabetic rats.