Background and Objective:
Binding of antibiotics to nanoparticles increases the antibacterial potential of nanoparticles and antibiotics. This study was performed to determine the antibacterial and hemolytic effect of zinc / ferrite / cellulose nanocomposite (ZnFe2O4 @ Cell) (single nanoparticle), zinc / ferrite / cellulose nanocomposite was aminated with 3-aminopropyltriethoxysilane (APTES) with the name of ZnFe2O4@Cell@APTES (Coated nanocomposite) and ZnFe2O4@Cell@APTES@Van nanocomposite (coated nanocomposite bound to vancomycin) against gram-negative bacteria Escherichia coli
) and Pseudomonas aeruginosa
) and gram-positive bacterium Staphylococcus aureus
In this descriptive study, antibacterial-activity was evaluated by broth macro dilution method. Minimum inhibitory concentration (MIC) and minimum lethal concentration (MBC) were determined for E. coli
, S. aurous
and P. aeruginosa
. The hemolytic activity of nanoparticles was investigated by colorimetric method.
Nanoparticles did not have hemolytic activity. ZnFe2O4@Cell and ZnFe2O4@Cell@APTES@Van did not have a significant antibacterial effect against gram-positive and gram-negative bacteria, and vancomycin binding resulted in antibacterial-activity. ZnFe2O4@Cell@APTES@Van inhibited the growth of Gram-negative bacteria Escherichia coli and Pseudomonas aeruginosa. The growth of E. coli
was reduced to 85% at a concentration of 0.4 mg/ml and a concentration of 0.1 mg nanoparticles completely prevented the growth of P. aeruginosa
. The growth of gram-positive S. aureus
bacteria at a concentration of 0.3 mg/ml nanoparticles was completely stopped.
Vancomycin-modified nanocomposite has antibacterial-activity against both gram-positive and gram-negative bacteria and has the potential to overcome the antibiotic resistance of bacteria.