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Showing 2 results for M.Saeedi (M.Sc)

M.saeedi (m.sc), H . Baradaran (ph .d), M.a Hatef (m.d),
Volume 3, Issue 2 (Autumn & Winter 2001)
Abstract

ANCA are classes of antibodies or in reality autoantibodies usually produce in conditions that degradation of neutrophiles occurs. This is led to vascular damage. They are more common in rheumatic diseases such as Rheumatoid Arthritis (RA), systemic Lupus Erythematus (SLE) and vasculitic diseases especially Wegners’ granulomatosis (With specificity and sensitivity as high as 95%). We decided to design IFA-ANCA because this assay has high sensitivity and in with comparison other techniques such as ELISA & RIA is more economic and simple. On the other hand, screening test for determining ANCA is immunofluorescent assay. ANCA test establishment contains: Isolation of neutrophiles, fixation with Ethanol and Formalin, improvement of method and quality control by using of indirect immunofluorescence assay (IFA), 2 staining pattern are observed. Cytoplasmic (C-ANCA) or classic and prinuclear (P-ANCA) pattern. Established ANCA test by inter assay method had 95% reproducibility and 91% precision.
M.saeedi (m.sc), S.bakhshandeh Nosrat (m.d), E.ghaemi (ph.d), S.m.hedayat Mofidi (m.sc), F.kohsar (m.sc), N.behnampour (m.sc),
Volume 4, Issue 1 (Spring & Summer 2002)
Abstract

Toxoplasmosis is a disease caused by a ubiquitous, obligate intra cellular protozoan Toxoplasma Gondii. Based on serological evidences, Toxoplasmosis infection is a widespread disease with regard to this fact and because to the occurrence of congenital Toxoplasmosis infections due to asymptomatic infections in pregnant women, determination of the rate of Toxoplasma antibodies (IgG, IgM) before pregnancy and finding the involved factors in increasing Toxoplasmosis prevalence seems to be necessary. This study was a cross-sectional and descriptive study and sample population was the women referred to marriage consultation the prevalence of Toxoplasma antibodies (IgG, IgM) was determined by ELISA method. Blood sample were collected randomly from 300 women referred to the center for consultation in 2001 and they was transferred to the laboratory for antibodies determination. The results showed that the general prevalence to the positive cases based on high titer of IgG was 48.3% and of IgM was 11.7%. There was not any relation between positive cases and age, education, place for residence, job, keeping domestic animals (Except cat) vegetable consumption and wash-up, but there was a relation between positive cases of IgM and keeping cat at home (P?0.025). More over 51.7% of pregnant women in Gorgan were seronegative and the were prone to acute Toxoplasmosis during their pregnancy. The results of this study confirm that the determination of the diagnostic Toxoplasmosis is an necessary test during pregnancy.

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مجله دانشگاه علوم پزشکی گرگان Journal of Gorgan University of Medical Sciences
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