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Background & Objective: Standard treatment in renal colic is established with narcotics. NSAIDS are alternative choices. Several studies are done on various treatments. This study was done to evaluate the effect of intranasal Desmopressin alone and in combination with parenteral Pethidine in relief of renal colic pain. Materials & Methods: In this clinical trial study, 150 patients with renal colic were recruited in: Group one received 40 micrograms intranasal desmopressin, group two 25 miligrams intravenous pethidine and in group 3 both drugs were administered. VAS method was used to measure the intensity of pain in first minute and at 10, 20 and 30 minutes after administration. Kruskal-Walis and ANOVA were used to compare the pain severity between groups. Results: Pain intensity had significant decrease after 10, 20 and 30 minutes in all groups (P<0.05). Group 3 showed maximum decrease after 30 minutes. Conclusion: This study determined that combined administration of desmopressin and pethidine can significantly decrease in renal colic intensity. We suggest the use of desmopressin, in combination with other medicines as a supportive and effective treatment in renal colic patients.

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Background & Objective: Preterm infants have less iron storage compared with the term one. Due to rapid growth they need more iron during infancy. This study was designed to evaluate the effect of early iron supplementation on hematologic indices and incidence of iron deficiency anemia in preterm infants. Materials & Methods: This experimental study was done on 20-days-old preterm breast-feed infants referred to the Gorgan-North of Iran primary health care services for vaccination, without any underline disease or growth retardation during 2005. They were divided to two groups (n=15 in each) and a questionnaire was completed for each case. Iron drop (2mg/kg) was given in the interventional group. Iron deficiency anemia was assesed in all sample T-student test and chi-square were used to analyze the independent variables and comparing the hematologic indices, after entering in SPSS-13 software and testing the normal distribution with komologrof-smearnoff test. Relative risk index (RR) was used to compare the two groups. Results: The relative risk of iron deficiency anemia in the interventional group was 0.4 folds [RR=0.4,CI %95 for RR=(0.091,1.749)]. No significant difference was seen between the two groups. Conclusion: The finding of this study showed that iron drop supplementation for preterm infant prevent the iron defeciency anemia, although this observation was not significant.

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Background & Objective: More than 50% of patients with hypertension will have end organ damage (such as: CHF, retinopathy ,CVA or renal failure).Left ventricular hypertrophy (LVH) is an independent risk factor for death and cardiovascular disease .Both renal dysfunction and LVH are signs of end organ damage so we carried out this study to evaluate the correlation between LVH in echocardiography and kidney function in patients with essential hypertension. Materials & Methods: This descriptive analytic study was carried out on 102 patients whome reffered to 5 Azar hospital in Gorgan- Iran, suffering from essential hypertension and had proved LVH in echocardiography with no other disease during 2005-6. Kidney and urinary tract assessment such as serum BUN, Creatinine level and kidney sonography was done on subjects. Left ventricular hypertrophy was defined as a left ventricular mass index (LVMI)>=135g/m2 in men and>=110g/m2 in women. Renal function was estimated by means of the serum Creatinine level and proteinuria and sonographic findings of kidney and urinary tract. Data was analysed by Spss-11.5 and Chi-Squre test. Results: The average age of patients were 60±8.8. 58%, 36% and 7% of patients had mild, moderate and sever LVH respectivly. The means of Creatinine and BUN level were 1.09±1.12 mg/dl and 20.6±10.76 mg/dl had direct correlation with kidney dysfunction (P<0.05). There was a significant relationship between LVH severity and presentse of Kidney scar in sonography (P<0.05). Conclusion: This study showed that a very large fraction of the subjects with essential hypertension have kidney problems. Therefore, serum BUN and Creatinine level and sonographic scar of kidney can be reliable markers for assessing and controlling target organ damages that are induced by Essential hypertension.

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Background & Objective: ADHD is the most common mental disorder in children. Awareness of students' mental health has an important role in programming for decrementing their disorders and, improving their mental health and also preventing the complications of their decreased mental health. The aim of this study was to determine the prevalence of ADHD and some of the affecting factors among school children in Nishaboor localed in the North – East of Iran. Materials & Methods: This descriptive study was done on school children in Nishaboor during 2006. Subjectes selected by stratified cluster sampling. Data collection was done using the Conner's Parent and Teacher Scale questionnaire, and each student's questionnaires were completed by both the teacher and the parents. Data analysis was performed regarding to the mean scores of teachers and parents. The score 15 was chosen as the probable indicator for ADHD. Results: 722 students (79.6%) were involved this study. Prevalence of ADHD was 12.5%, CI95%: 10–14.8%. There was no significant difference in gender distribution. The prevalence of ADHD had a significant rise with age (P<0.05). The highest prevalence was in spring born and the lowest was in the summer born school children (P<0.05). The prevalence of ADHD had a significant relationship with father's education and was lower in students whose father had higher educations (P<0.05). Variables such as type of delivery, pariety, mother's education had no significant relationship with the prevalence of ADHD. Conclusion: In this study the prevalence of ADHD is higher than similar studies. Risk groups to be followed up by the responsible organizations.

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Background & Objective: The atherosclerosis of left anterior descending (LAD) coronary artery because of subtending more than 50% of left ventricular myocardial mass, has long been the focus of concern regarding appropriate treatment. This study aimed to evaluate efficacy of Percutaneous Transluminal Coronary Angioplasty (PTCA) with stenting for treatment of proximal LAD disease. Materials & Methods: In this descriptive study, 75 patients with isolated proximal LAD atherosclerotic lesion treated with PTCA and stenting were followed up for 9 months. The stenosis of all patients was documented by coronary angiography prior to enrollment. Exercise tolerance testing (ETT) by Bruce protocol was performed before angioplasty and at the end of follow up period as well. Results: There were 44 (58.7%) males, and 31 (41.3%) females. The mean age of patients was 59.4 (range, 39 to 83 yrs). Clinical outcomes of 73 (97.3%) patients were satisfactory. Only two patients developed re-stenosis during the follow up period. The first patient developed myocardial infarction at the 3rd day of her admission, and the second one had an abnormal ETT after 9 months. Percutaneous coronary revascularization with stenting was performed, and both of them were discharged without any problem. Conclusion: This study demostrated that the nine months prognosis of patients with isolated stenosis of proximal LAD coronary artery that treated by PTCA and stenting was acceptable. This treatment improves clinical status and results in lower hospital adverse event rates and in appropriate clinical outcome.

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Background and Objective: Short stature among children is often due to physiological causes but it can be a sign of important and curable diseases. Thus early detection of problem, before epiphyseal closure, is critical. This study was done to determine the cause of short stature among children in Gorgan-Iran. Materials and Methods: This cross- sectional research study was done on 100 children of 6-14 years of age during 2005 in Gorgan-Iran. Children were selected by census. Demographic character of children, history of serious disease, prematurity, blood and provocative growth hormone tests, physical examination, wrist radiography and standard deviation score (SDS) were recorded for each child. Data were analyzed by t-student and chi-square tests. Results: The finding of this study showd that most children with short stature was girl (66%). mean+-SD of calendar and bone age of female children was 10.4+-2.25 and 8.4+-2.32 year, respectively. The most frequent causes of short stature were constitutional (57%), Growth hormone deficiency (30%) and familial causes (8%). Mean+-SD of calendar and bone age of males children was 10.79+-2.32 and 7.89+-2.19 years. Children with growth hormone deficiency had lower bone age and children with constitutional short stature had higher standard deviation score than others. (P<0.05). Conclusion: This study showed that since growth hormone deficiency was one of the common and important pathologic causes of short stature. Therefore growth hormone determination and theraphy is recommended, after elimination of familial and constitutional factors.

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Background and Objective: The growing development progress of electronic industries and the increasing use of electrical appliances have led to higher rate of exposure of people to electromagnetic field (EMF). Thus, in this study we investigated the effect of EMF on morphometric indices of epididymis and vas deferen in mice. Materials and Methods: In this experimental study, 30 BALB/c male mice were selected and divided into three control, sham and experimental groups. Experimental group were exposed to 50 Hz, 0.5 mT EMF for 4 hours per days, 6 days per week for 8 weeks while the animal in control and sham groups were not exposed to EMF. After the exposure period, the mice were dissected and left testis was removed and weighted. Samples of epididymis and vas deferen in all groups were taken and were processed for routine light microscopic studies. The diameters of epididymis and vas deferen and the height of epithelial cells in all groups were compared using ANOVA and Tukey tests. Results: The mean diameter of epididymis in EMF group significantly decreased compared to the control group (P<0.05). The mean diameter of vas deferen, the height of epithelial cells in epididymis and vas deferen in EMF groups significantly decreased compared to the control and sham groups (P<0.05). In addition, the weight of testes in EMF group significantly decreased compared to the control and sham groups (P<0.05). Conclusion: This study showed that the EMF exposure for long time could have hazard effect for the male reproductive system by decreasing the diameter of reproductive ducts, the length of epithelial cells and weight of testes.

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Background and Objective: The increase of nosocomial systematic fungal infections due to pathogenic yeast, led to researchers on finding novel antifungals with potent inhibitory activity toward a wide range of pathogenic fungi. In the present study, antifungal effect of aqueous garlic extract individually and in combination with Fluconazole, Itraconazole and Ketoconazole were studied against some pathogenic yeasts. Materials and Methods: Broth microdilution method was used for evaluating antifungal activities of aqueous garlic extract with 0.03-256 µg/ml individually and in combination with Fluconazole, Itraconazole and Ketoconazole against Candida albicans PTCC5057, Candida dubliniensis CD36, Cryptococcus neoformance CNE1 and Malassezia furfur MF1, in vitro. The microdilution method was used for assessing antifungal susceptibility of above-mentioned compounds in two culture media sabouraud dextrose broth (for all fungi except M.furfur) and modified Dixon broth (for only M.furfur). The minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of aqueous garlic extract and antifungal drugs tested were determined by on comparison of colony forming units (CFU) between test and control groups. Results: Aqueous garlic extract inhibited the growth of all fungi tested in a dose-dependent manner, in a concentration comparable with azole drugs.The MIC ranges of aqueous garlic extract, Candida albicans, Candida dubliniensis, Cryptococcus neoformances and Malassezia furfur was determined to be 0.25-64 g/ml. The MIC ranges of aqueous garlic extract in combination with Fluconazole was determined 0.125-8, 0.25-16, 0.125-16 and 0.5-8 µg/ml, respectively. The MIC ranges of aqueous garlic extract in combination with Itraconazole was determined 0.25-8, 0.125-2, 0.125-16, 0.25-4 µg/ml, respectively.The MIC ranges of aqueous garlic extract in combination with Ketoconazole was determined 0.125-4, 0.125-1, 0.125-8 and 0.125-2 µg/ml, respectively.The results indicated that the antifungal activities of drugs were increased in combination with aqueous garlic extract (P<0.05). Conclusion: This study showed that, the aqueous garlic extract increased the antifungal activity and decreased MIC of drugs in combination with them.

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Background and Objective: Hemodialysis is one of the common therapulic proceduce for terminal renal failure. Insufficient and low efficient hemodialysis increases the mortality rate, therefore the assessment of hemodialysis efficacy is important. This study was done to determine the hemodialysis efficacy based on urea reduction ratio (URR) model and kt/v (Clearance Time Volume) criteria in Gorgan, Northern Iran. Materials and Methods: In this descriptive and analytical study 113 heomdilysis patients were selected prior to hemodilysis and five minutes after pump stopping, arterial blood urea nitrogen (BUN) was measured and then kt/v and URR were calculated. Also kt/v relations with weight, gender, educational level, length of hemodialysis session, blood flow rate, TMP (Terans Membran Presure), filter Ku/f (Clearance Ultrafiltration), preliminary disease, time of hemodialysis per week and the type of vessel access were determined. Data analyzed using SPSS-13 and independent t-test, chi-square, fisher exact test and Pearson correlation coefficient. Results: The mean of hemodilysis history was 37 months and mean of Patient's ages were 51.6 years. 77.9% of subjects had hemodialysis efficacy according to Kt/v and URR critrial. There was a statistical significant correlation between hemodialysis efficacy and time of hemodialysis session, blood flow rate, time of hemodialysis per week and type of vessels access (P<0.05), but there was not any significant correlation between hemodialysis efficacy and weight, gender, education, TMP, filter Ku/f and preliminary disease. Conclusion: This study suggested that hemodialysis efficacy in this center was better that other studies in Iran.

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Background and Objective: Diagnosis of latent tuberculosis infection (LTBI) by tuberculin skin test (TST) is accompaind with limitations. A few documented data exist regarding the performance of serology tests for LTBI diagnosis in HIV infection. The aim of this study was to determine LTBI prevalency and compare TST results and IgM antibodies against M.tuberculosis antigens (IAMA) for the diagnosis of LTBI in HIV infected individuals. Materials and Methods: In this discriptive study, sixty two HIV infected subjects were chosen randomly from an addict treatment center in Ahvaz southwest Iran during 2008. The patients underwent TST, using 5 TU of purified protein derivative, and IAMA was measured. Data were analyzed using SPSS-15 software and Chi-Square test. Results: From 62 studied cases, 34 (54.8%) had positive result for TST, whereas 6 (9.7%) had positive IAMA. LTBI was diagnosed in 37 (59.7%) by either TST or IAMA. Overall concordance between TST and IAMA was 45.2%. In subjects with positive test by either TST or IAMA, only 4.8% had positive test by either diagnostic procedure. Discordant results were found in 54.8% of subjects. Positive results for both diagnostic methods in subjects with above and below 200 CD4-cell/mm3 did not show any significant differences. Conclusion: This study showed that LTBI prevalence among HIV infected individuals among addict patients, in this region is higher than other parts of the world. Also TST is a useful test for LTBI diagnosis is preferable to IAMA method.

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Background and Objective: Carbamazepine (CBZ) is an antiepileptic drug that causes significant malformations such as neural tube defects (NTDs), cardiac, skeletal and craniofacial defects if it is consumed during pregnancy. The aim of this study was to evaluate the protective effect of folic acid on prevention of birth defect due to Carbamazepine in Balb/c mice. Materials and Methods: In this experimental study, Sixty Balb/c timed-pregnant mice were divided into 4 experimental and 2 control groups. Two experimental groups received daily intraperitoneal injections of 30 mg/kg (group I) and 60 mg/kg/body weight (group II) of CBZ on gestational days (GD) 6 to 15. Two other experimental groups (group III and IV) received similar doses of CBZ with folic acid supplement (3 mg/kg/day) by gavages route for 10 days before pregnancy and 15 days after GD0 (gestational day 0). Two control groups received normal saline or Tween 20 (polysorbate 20). Dams underwent cesarean section on GD18 and embryos were collected. External examination was done and data concerning malformations, weight and crown- rump of fetuses were collected and analyzed by using SPSS-11.5 software and ANOVA and chi-square tests. Results: The mean weight and crown-rump of the fetuses in both experimental groups I and II were significantly reduced. Also in both experimental groups I and II various malformations were detected such as open eyes, limb defects, scoliosis, facial deformity and NTDs. The mean weight and crown-rump of fetuses in the folic acid treated groups did not show any meaningful differences in comparison with fetuses in experimental groups I and II. Also, meaningful reductions in eye, vertebral, limb and facial defects were seen in fetuses of group III. In experimental group IV, reduction of vertebral and limb defects were observed. Conclusion: This study showed that consumption of folic acid (3 mg/kg/body weight) before and during pregnancy can reduce birth defects due to CBZ in Balb/c mice fetus.

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Background and Objective: Morphological alterations of hippocampus and dentate gyrus due to opium were reported in humans and animals. Also other evidences have shown that astrocytes actively participate in synaptic plasticity. This study was done to determine the conditioning place preference (CPP) on astrocytes number of Rat dentate gyrus by immunohistochemical technique.

Materials and Methods: In this experimental study, 48 male Wistar Rat weighted average 220-250 g were used. For behavioural tests, Rats divided into eight experimental groups. The Rats were received morphine at different doses (2.5, 5, 7.5 mg/kg) for three days by subcutaneous injection and sham groups, received saline dose (1 mg/kg) and then CPP test in them were investigated. 48 hours after behavioural testing animals were decapitated under chloroform anesthesia and their brains fixed and after tissue processing, slices stained with immunohistochemistery techniques. For morphometric study PTAH staining of astrocytes was used.

Results: The most dose responses of morphine was observed in 7.5mg/kg. The number of astrocytes in the controls (20.627±6.129) was similar to control-saline group (17.339±4.71). This difference was not significant, while the difference in the number of astrocytes in control group with morphine-treated experimental groups was significant (P<0.05).

Conclusion: We concluded that the phenomenon of conditioned place preference induced by morphine can cause a significant increase in the number of astrocytes of sham and experimental groups compared to controls.

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Background and Objective: Cardiovascular diseases are among 46% of deaths in Iran. Changing lifestyle is very important for the disease control. If the patients perceive the benefits of preventive behaviours, the barriers to those behaviours will decline and the probability of engaging in the behaviours will increase. This study carried out to determine perceived benefits and barriers of preventive behaviours of relapsed myocardial infraction.

Materials and Methods: This cross-sectional study was carried out on 100 patients with a history of myocardial infraction referred to Yazd Cardiovascular Research Center during 2007. A questionnaire was used for data collection which measured perceived benefits of, barriers to preventive behaviours of myocardial infraction relapse and status of those behaviours. Data was analyzed using SPSS-13 and Pearson coefficient and regression tests.

Results: Subjects earned a mean score of 30.24 (out of 36) in perceived benefits and 22.07 (out of 68) in perceived barriers and 24.48 (out of 37) in preventive behaviours. Preventive behaviours was positively correlated with net benefit and negatively with perceived barriers.

Conclusion: This study showed that in spit of suitable level of perceived benefits of myocardial infraction, but still the perceived barriers are significant enough and therefor removal of barriers to such behaviour are essential.

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Background and Objective: Anterior cruciate ligament (ACL) tear is one of the most common injuries at knee joint. This study was done to evalute the effect of reconstruction surgery on hamstring reflex in patients with ACL tear. Materials and Methods: In this Quasi- experimental study, 30 patients (16 males, 14 females) with ACL tear, with mean age of 26.52±8.72 years old were recruited during 2007. Patients were selected in a non probability sampling manner. The Kinsiological Electromyography and trauma mechanism were used for testing the hamstring reflex. Muscle activity were measured as a dependent variable. Data were analyzed with Paired T-Test, ICC, SEM and K-S tests. Results: Hamstring reflex in affected knee and after reconstruction surgery was 73.25±3.22 and 47.35±3.85, respectively. This difference was significant (P<0.05). Conclusion: Reconstruction surgery in patients with ACL tear at acute phase is effective in improvement of hamstring reflex.

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Background and Objective: The majority of medicines are associated with some form of side effects due to high prevalence of self- medication in Iran .There is not any drug without side effects the prevalence of self- medication in Iran's provinces is increasing. This study was done to determine the knowledge and practice among woman about self -medication based on health belief model. Materials and Methods: This cross- sectional study carried out on 385 women whom referred to health centers in Isfahan, Iran during 2008. Data were collected by a questionnaire including knowledge, practice and health belief model structures. Results: The mean score of knowledge was 82.97±13.80 and means score of perceived susceptibility 72.48±17.11, severity 71.60±14.73, benefits 68.29±17.13 and barriers 61.64±19.67. 86% of women have done self–medication at least in one disease in 6 months earlier Including: cold 225 (57.6%), headache 28 (53.7%) and anemia 51 (13.2%). The main reasons for self –medication were previous experience and medicine accessibility .The main reasons to avoid self –medication was the side effects of medicine. Conclusion: Self-medication can be prevented thought increasing knowledge and education about severity of drug side effects.

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Background and Objective: Considering the relatively wide application of ceftriaxone in pediatric infectious diseases and its side effects, this study was done to determine gallblader sonographic abnormality following ceftriaxone treatment in children.

Materials and Methods: This descriptive cross- sectional study was carried out on 60 patients age 1 month up to 12 years in Besat hospital, Hamadan, Iran during 2007. The sonographic abnormal finding of gallbladder before ceftriaxone therapy and 5 days after therapy were recorded. In case of any abnormality in gallblader sonography was repeated twice a week in the first two weeks and afterward once a week up to disappearance of abnormalities.

Results: Gallblader sonographic abnormality were observed in 10 cases (16.5%). Out of them, 8 and 2 patients had bile stone and bile sludge, respectivley. The patients did not show any clinical manifestations. There was no relation between age and sex with abnormal findings. Gallblader abnormality completely were disappeared in the worst cases by sixteen days.

Conclusion: This study showed that the incidence of either gallstone or biliary sludge after treatment with ceftriaxone was 16.5% which is relatively similar to other studies.

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Background and Objective: Regarding to probable high frequency of Glucose - 6 - phosphate dehydrogenase (G6PD) deficiency in Iran, screening of all neonates by cord blood is under consideration. This study was conducted to determine the prevalence of G6PD deficiency in newborns and the relation between gender, jaundice, hemolysis, anemia and the G6PD deficiency in neonated born in in Tehran, Iran.

Materials and Methods: In this descriptive study, cord blood of 450 neonates born in Akbarabady hospital in Tehran, Iran during 2008-09 were screened. Demographic information was recorded by questionnaires and the newborns were examined for detection of jaundice till discharge. G6PD level was determined by Fluorescent Spot Test (FST). G6PD deficient neonate were put under close observation for detection of jaundice. Enzyme activity was rechecked by spectrophotometry.

Results: Nine neonates out of 450 were G6PD deficient (8 boys and one girl). Prevalence of G6PD deficiency was 2% (3.3% for boys and 0.5% for girls). Six neonates of nine G6PD deficient neonates (66%) developed pathologic jaundice. Four neonates were managed by phototherapy and two by exchange transfusion.

Conclusion: This study showed that G6PD deficiency is more prevalate among male neonates, therefore, G6PD determination is recommended to prevent the possible neonatal jaundice.

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Background and Objective: Glaucoma is one of the most important cause of blindness wordwide. Exact determination of intra ocular pressure is important for the diagnosis and decision making about glaucoma treatment. Central corneal thickness is considered as effective factor on intra ocular pressure and visual field defect. This study was carried out to determine the relationship between central corneal thickness, intra ocular pressure and visual field in normal tension and primary open angle glaucoma. Materials and Methods: This descriptive study was carried out on 45 eyes with normal tension glaucoma and 45 eyes with primary open angle glaucoma in Al-Zahra ophthalmology hospital in Zahedan, Iran during 2010. Intra ocular pressure and central corneal thickness were measured by Goldman tonometer and pachymeter and visual field exanimated by Humphrey perimeter. Data were analyzed using SPSS-16, paired t-test, ANOVA, Mann–Whitney and Pearson corlateion tests. Results: There was significant correlation between central corneal thickness and intra ocular pressure (r=0.309, P<0.05). A significant difference was detected in intra ocular pressure between two type of glaucoma (P<0.05). Mean value of central corneal thickness in patient with mild visual field defect was higher than severe visual field defect but there was not significant statistical difference between central corneal thickness and visual field defect in subjects with glaucoma. Conclusion: This study indicated that increasing corneal thickness is accompanied with intra occular presure.

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Background and Objective: Although acquired immune deficiency syndrome (AIDS) is a vital medical problem but it is more prominant as a personal behavior social cultural phenomenon. Adolescents have been identified as risk population in immune deficiency virus. Health education is only effective method to prevent AIDS. This study was carried out to determine the effect of educational program on knowledge and health belief model structures about acquired immune deficiency syndrome (AIDS) among high school female student in Isfahan, Iran. Materials and Methods: This quasi-experimental study conducted on 72 female students whom divided equaly into case and control groups. The questionnaire included demographic characteristics, knowledge and health belief model. Educational interventions were performed in lecture and group discussion. Data were analyzed using SPSS-18, paired t-test and independent t-test. Results: There were no significant differences in knowledge, perceived severity, benefits and barrier in two groups before intervention. After intervention, there were significant increases in knowledge, perceived severity, perceived benefits and perceived barriers (P<0.05), in the intervention group, but there was not significant increases in perceived susceptibility. Conclusion: Education based on health belief model can improve knowledge, perceived severity, benefits and barriers in female student. More educational sessions are required for improving perceived susceptibility in high school female students.

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Background and Objective: Nanosilver particles are one of the functional nanotechnology filed. These nanoparticles have antibacterial characteritics. Combination therapy is one of new and specific therapeutic regiment in medicine. Eucalyptus plant is useful in growth inhibition of bacteria. This study was done to evaluate the inhibitory effect of alcoholic eucalyptus extract with nanosilver on E.coli growth. Materials and Methods: In this laboratory study, E.coli was cultured in nutrient agar medium. Primarily 50 l of bacteria was inocolulated in each plate, antibiograms disc contiminated with 3.1, 6.25, 12.5, 25 and 50 ppm concentration of nanosilver accompanied with 100% of etanolic extract of eucalyptus were placed in each plate subsequently. Diameter of inhibitory zone were evaluated following 24, 48, 72 hrs in 6th and 8th days. Data were analyzed using SPSS-15, ANOVA and Tukey tests. Results: After six days, inhibitory growth zone diameter of E.coli was 0.5 mm. This diameter in experimental group with 3.1, 6.25, 12.5, 25 and 50 ppm of nanosilver in combination with etanolic extract of eucalyptus were 0.55, 0.58, 0.82, 0.83 and 1.02 mm. Inhibitory growth zone in group of 12.5, 25 and 50 ppm of nanosilver was significant in compared to the control (P<0.05). Conclusion: The most proper time of inhibitory effect on E.coli growth is six day after treatment in combination of 50 ppm nanosilver particles with extract of eucalyptus.