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Background and Objective: Immobilization stress has a variety of effects on the enzymes activity. This study was conducted to determine the protective effect of Aloe vera extract on the serum level of creatine kinase enzyme in male rates exposed to acute and chronic immobilization stress. Materials and Methods: This experimental study was conducted on 45 male Wistar rats weighing approximately 200±30g. Animals were randomly allocated into 9 groups of 5 rats: control, normal saline, chronically immobilized, acutely immobilized, chronically immobilized normal saline, acutely immobilized normal saline, Aloe vera extract (600mg/kg/daily), acutely immobilized Aloe vera (600g/kg/daily) and chronically immobilized Aloe vera groups (600g/kg/daily). Aloe vera extract with a dose of 600mg/kg/BW was administered by gavage feeding before applying stress. For chronic immobilization, animals were put under immobilization stress for 2 hrs a day for 3 weeks and for acute immobilization animals were put under immobilization for 8hrs a day for one week. At the end of the experiments, blood samples were collected using cardiac puncture method and serum level of creatine kinase enzyme (units/L) was measured by spectrophotometery. Data were analyzed using SPSS-19, one-way ANOVA and Tukey post-hoc tests. Results: Serum level of creatine kinase enzyme represented a statistical significant increase in rats exposed to acute (2368.20±104.96 units/L) and chronic immobilization (2177.80±234.75 units/L) compared with control group (1240.40±706.40 units/L) (P<0.001). The enzyme alteration level was not significant in Aloe vera (1619.80±171.41 units/L), acutely immobilized Aloe vera extract (1619.00±206.03 units/L) and chronically immobilized Aloe vera extract (1448.00±106.07 units/L). Conclusion: This study showed that gavage of Aloe vera extract (600mg/kg/daily) in rats can prevent the elevation of creatine kinase enzyme activity resulted by immobilization stress.

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Background and Objective: Diabetes mellitus cause learning, memory and cognitive skills disorders in the long term. This study was conducted to determine the protective effect of silymarin on the learning and memory deficiency in streptozotocin-diabetic rats. Materials and Methods: This experimental study was conducted on 40 male Wistar rats weighing 240-300 grams. The rats were randomly allocated into 5 groups: control, silymarin -treated control (100 mg/kg), diabetic, and two silymarin -treated diabetic groups (50 and 100 mg/kg). Silymarin was daily administered (i.p. and daily) ten days after streptozotocin injection for 4 weeks. Finally, initial (acquisition index) and step-through latencies (retention and recall index) were measured using passive avoidance test and alternation behavior percentage as an index of spatial memory was determined using Y maze. The level of malondialdehyde in the homogenate hippocampal tissue of the animals brains was measured. Data were analyzed using Sigma Stat-3.5, one-way and two-way ANOVA, Tukey, and Kruskall-Wallis tests. Results: A significant reduction of STL was observed in diabetic (P<0.01) and silymarin-treated (50mg/kg) diabetic (P<0.05) groups and this parameter was significantly higher in diabetic group receiving a high dose of silymarin compared to diabetic group (P<0.05). Meanwhile, alternation percentage in diabetic animals was significantly lower than control group (P<0.05) and this index did not show a significant difference in silymarin-treated diabetic groups in comparison with diabetic group. In diabetic rats, there was a significant increase in the tissue level of malondialdehyde (P<0.05) and silymarin treatment with dosage of (100 mg/kg) significantly reduced the level of MDA (P<0.05). Conclusion: This study showed that although long-term administration of silymarin at a high dose (100 mg/kg) affects the ability to store data in memory and to recall it in diabetic animals in passive avoidance test, it does not improve short-term spatial memory in diabetic animals. The beneficial effects of silymarin may be via attenuation of lipid peroxidation in hippocampus tissue.

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Papillon-Lefevre Syndrome is a rare autosomal recessive disorder. This syndrome accompanied by palmoplantar hyperkeratosis and severe periodontal destruction of primary and permanent teeth. The teeth erupt normally but due to the severe alveolar bone loss both in deciduous as well as permanent dentitions, these teeth are exfoliated within two or three years after eruptions and by the age of 15 or 17. Patients are usually edentulous. Due to periodontal disease, the dentists are often the first ones who diagnose the syndrome. A 15-year-old girl was referred to dental clinic complaining of permanent teeth mobility. All patient’s teeth except 13, 14, 17, 23, 27, 37, 43, 44 and 47 had been extracted. The third molars were impacted. Patient has advanced periodontal disease and all teeth have mobility. There was hyperkeratosis at the palms and soles. The teeth were extracted and treated with complete denture. Early diagnosis of the papillon-lefevre syndrome can help to preserve teeth. Dental treatment included extraction of all deciduous teeth, professional prophylaxis, conventional periodontal therapy, systemic antibiotics, oral retinoid, complete dentures and implants.

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Background and Objective: The Detection of neurologic signs for topographical disorders in central nerves system can prevent unnecessary neuroimaging techniques such as MRI. This study was performed to determine the diagnostic value of physical examination for topographic detection of infratentorial lesions in patients with cerebrovascular syndromes. Methods: This descriptive study was done on 200 patients with cerebrovascular syndrome in Qaem Hospital in Mashhad, north-east Iran during 2011. Regarding to topographic physical examination, sensitivity, specificity, positive and negative predictive value and accuracy of signs of syndromes were compared to MRI as gold standard method. Results: The accuracy of tetraparesis and crossed syndromes for localization of brain lesion in brainstem was 79% and 83%, respectively. The accuracy of hemiataxia for localization of brain lesion in ipsilateral cerebellar hemisphere was 98%. The accuracy of Wallenberg syndrome for localization of brain lesion in posterolateral medulla was 98%. Conclusion: Using clinical neuro-examination skills and accurate topographic physical examination can prevent unwanted MRI technique for the diagnoses of cerebrovascular syndromes.

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Background and Objective: Paraoxonase-1 is an important factor in preventing lipid oxidation and formation of oxidized low-density lipoprotein. There are conflicting reports on the impact of physical activity on serum level of Paraoxonase-1. This study was done to determine the effect of four weeks of endurance training on serum level of Paraoxonase-1 and lipid profile in non-athlete obese men. Method: In this clinical trial study, sixteen obese healthy non-athletic men randomly divided into intervention and control groups. Subjects in interventional group were practiced endurance running periodic for four weeks, three sessions a week with 65-80% of maximum heart rate. Blood samples collected 48 hours perior the first and 48 hours following the final training. Serum level of Paraoxonase-1 and lipid profile including cholesterol, triglycerides, low-density lipoprotein and high-density lipoprotein were measured by ELISA method. Results: At the end of the training, the serum level of of paraoxonase -1 increased 15.57% (P<0.05) in interventional group and non-significantly reduced in control group (19.25%). The serum level of serum Paraoxonase-1 in interventional group significantly increased compared to controls (P<0.05). The serum level of low-density lipoprotein in interventional group significantly reduced in comparision with controls (P<0.05). Conclusion: 4 weeks of endurance training increased serum level of paraoxonase -1 and decreased low-density lipoprotein in non-athlete obese men.

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Background and Objective: Ruta graveolens is a plant used in traditional medicine with variety of therapeutic effects. This study was done to evaluate the antimicrobial effect of hydro and alcoholic extracts of Ruta graveolens on 8 species of pathogenic bacteria. Methods: In this descriptive study, standard strains of Enterococcus faecalis, Staphilococcus aureus, Staphilococcus epidermis, Strptococcus pneumonia, Escherichia Coli, Klebsiella pneumonia, Salmonella typhi and Enterobacter Cloace were obtained from Iranian institute of scientific and industerial research center. The effect of hydro and hydroalcholic extracts of Ruta graveolens on the growth of bacteria was determined by disc diffusion and serial macro dilution methods. Minimum inhibitory concentration (MIC) for each extract was determined and the findings were compared with 8 common antibiotics including Co-trimoxazole, Oxycycline, Vancomycin, Erythromycin, Furazolidon, Tetracycline, amoxicillin / clavulanic acid and Ampicillin/sulbactam. Results: Hydro and hydroalcoholic extracts of Ruta graveolens did not show any inhibitory effect on growth of the pathogenic bacteria even in the presence of high concentration (3mg/ml). Conclusion: The extracts of arial organs of Ruta graveolens had no inhibitory effect on growth of the common human pathogenic bacteria.

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Background and Objective: Rotaviruses are the members of the Reoviridae family containing double-stranded RNA (dsRNA) genome which are the main cause of gastroinentritis particularly in children less than three years. This study was designed to evaluate the detection of rotavirus genome by new silver staining method using polyacrylamide gel electrophoresis (PAGE). Method: In this descriptive study, the samples were collected from infected MA-104 cell culture and the RNA electrophoresis was performed in 10% polyacrylamide slab gels after RNA extraction. Results: According to polyacrylamide gel electrophoresis and sensitive staining analysis, rotavirus RNA segments were divided into 4 groups and single-nucleotides differences were clearly detected rapidly. Conclusion: New silver staining method using polyacrylamide gel electrophoresis has the capacity to detect the rotavirus electeropherotype within a few minutes even in small DNA/RNA pieces up to 7 picograms.

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Background and Objective: MIRU-VNTR typing is currently one of the most frequently-used standardized genotyping systems in molecular epidemiology of tuberculosis in the world. This sudy was done to determine the Mycobacterium tuberculosis genotyping by MIRU-VNTR method. Methods: This descriptive study was done on sputum, gastric lavage clinical specimens of 53 tuberculosis suspected patients. Fifty-three isolates were identified by 16S rRNA and Rv-typing followed by RD typing. They were then subjected to a 12-locus (ETRA, ETRB, ETRC, ETRD, ETRE and ETRF, MIRU-10, MIRU-26, MIRU-39, MIRU-30 plus QUB-11b) MIRU-VNTR typing system. Results: In MIRU-VNTR typing, forty-four types were identified with 13 isolates classified in 4 clustered and the remaining 40 isolates representing 40 orphan patterns. In comparative analysis of MIRU-VNTR loci, MIRU-26 with 7 alleles displayed the highest diversity level (Simpson’s diversity index = 0.767. Out of the 53 isolates, only one was identified as Mycobacterium bovis. All the remaining isolates were characterized as Mycobacterium tuberculosis. None of the samples was affected to Mycobacterium complex strain. No evidence of either double or co-infection of the patients with more than one species/strain was detected. Conclusion: While the genomic diversity observed by MIRU-VNTR typing sounds extensive, the population genomic structure on the whole however, seems to be homogenous. Recent transmission between studied patients does not appear to be a frequent event as only 13 isolates representing 4 MIRU-VNTR types, were assumingly epidemic.

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Background and Objective: Bacterial resistance to Imipenem is increased in bacterial infections in Iran. In regard to the importance of Imipenem in the treatment of nosocomial infections and the key role of disc diffusion method as a major antibiotic susceptibility testing assay, this study was done to determine the prevalency of imipenem-resistant bacterial strains isolated from hospital and accuracy of Iranian imipenem disc product. Methods: In this descriptive-analytic study, 241 bacteria were isolated from patients in different wards of the Baqyatallah hospital in Tehran, Iran during 2013-14. After streaking of the organisms, identification was performed by all conventional biochemical tests. The bacterial resistance to imipenem was determined by disk diffusion method using Iranian and Mast imipenem discs. True imipenem-resistant isolates were examined for susceptibility to six different antibiotic including Ciprofloxacin, Gentamicin, Cephalexin, Azitromysin, Tetracycline and Ceftazidim, using disk diffusion method. Results: The most prevalent isolates organisms were gram-negative bacilli (Klebsiella, Escherichia coli, and Pseudomonas aeruginosa, respectively). The common clinical source was urine and wound samples, respectively. Resistant to Imipenem was 68 (25.7 %) and 19 (7.8 %) based on the results of Iranian and Mast Imipenem discs, respectively. False results for Iranian Imipenem discs was higher than Mast Imipenem discs (P<0.05). Among the 19 true Imipenem resistant isolates, 17 micro organisms were Pseudomonas aeruginosa. 57% of isolated resistant to Imipenem were isolated from ICU ward. The most resistance was seen to Gentamicin (84%) and the lowest was seen to Ciprofloxacin (63%). 84% of isolated samples were multi drug resistance. Conclusion: Although a small percentage of the isolates obtained as important nosocomial pathogens were resistant to Imipenem, but the rate of multiple resistance and high rate of isolates obtained from ICU was noticeable.

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Background and Objective: In psychotherapy, mindfulness is intended as a mode promotion of self- awareness think to improve well-being. This study was done to determine the effect of mindfulness training on reduction of depressive symptoms among students. Methods: In this quasi-experimental study, 40 female students with depressive symptoms were divided into mindfulness training and control groups. Subjects in experimental group received training mindfulness-based stress reduction and the control group did not receive any training. Training was performed eight sessions for experimentl group. Subjects completed the beck depression questionnaire prior and after the training. Results: The mean±SD of depression symptoms was 21.10±5.6 but after mindfulness training significantly reduced to 11.5±3.70 (P<0.05). Conclusion: Mindfulness training reduces depression symptoms among female students.

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Background and Objective: Wound infection treatment, particularly in chronic and bacterial poly cases, is difficult and entails heavy costs. This study was done to determine the prevalence of poly bacterial infection and antimicrobial susceptibility of wound samples from different wards. Methods: In this descriptive study, wound sampling was prepared from 336 patients admitted to different wards of Baqiatallah Hospital in Tehran, Iran. Identification was performed based on biochemical tests including oxidase test, TSI, IMVIC, lysine decarboxylase, phenylalanine deaminase, urea, motility, catalase, coagulase, mannitol fermentation, optochin sensitivity, susceptibility to bacitracin and sulfamethoxazole, growth in Bile esculin and DNase production. Antibiotic resistance pattern of isolates was determined using disk diffusion method for 14 important antibiotics. Results: 294 samples were positive for bacterial culture, from which 364 isolates including 11 different isolates were obtained. Out of 294 positive samples, 245 samples were mono bacterial and 54 were poly bacterial including two-bacterial (45 samples), three-bacterial (7 samples), and four-bacteral (2 samples). S. aureus (29.7%), Enterococci (15.6%), and E. coli (15.6%) were the most prevalent isolates. S. aureus-Enterococci pattern was the most common two-bacterial pattern (33%), and majority of polybacterial patterns belonging to gram negative bacteria was in surgery ward (32.5%). Antibiogram results showed high levels of antibiotic resistance in the isolates. Imipenem and amikacin were the most effective antibiotics against Gram negative isolates, and vancomycin for Gram positive isolates. Also, 71% of S. aureus isolates were resistant to oxacillin. Conclusion: Variation of bacterial isolates was similar to other studies. Most of poly-bacterial wound infections were due to common nosocomial pathogens and their high rates of antibiotic resistance are extremely alarming.

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Background and Objective: Acetaminophen is a commonly used analgesic and antipyretic agent which, in high doses, causes liver and kidney necrosis in man and animals. Carthamus tinctorius L. (Safflower) is a colorful and cheap plant that used often instead of saffron. In this study, protective effects of Carthamus tinctorius L. aqueous extract on acetaminophen induced nephrotoxicity in mice were investigated.

Methods: In this experimental study, forty adult NMRI male mice were randomly divided into five groups. Group 1 (control group) received normal salin for 15 days. Group 2 received 300 mg/kg Carthamus tinctorius L. extract for 15 days. Group 3 received normal salin for 15 days and 500 mg/kg acetaminophen was given in 15^th day. Groups 4 and 5 received daily 150 and 300 mg/kg Carthamus tinctorius L. extract for 15 days, respectively, and acetaminophen was also given in 15^th day. In 16^th day, blood samples were taken for BUN (Blood Urea Nitrogen), Cr (Creatinine) and Uric acid tests, and the mice kidneys were removed for histopathology assessments.

Results: Acute renal necrosis and BUN, Cr and Uric acid levels were significantly increased in acetaminophen treated mice (P<0.05). BUN, Cr and Uric acid levels were significantly reduced in the Carthamus tinctorius L. treated groups in comparison to acetaminophen group (P<0.05) and this reduction was greater in group 5. Carthamus tinctorius L. extract also reduced tubular necrosis-induced by acetaminophen.

Conclusion: Carthamus tinctorius L. extract have protective effects on acute renal injury induced by acetaminophen.

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Background and Objective: Aging reduces cardiac autonomic function parameters such as heart rate response to exercise and heart rate recovery after physical activity. This study aimed to determine the parasympathetic nerves reactivation after upper body exercise among young and middle-aged men.
Methods: In this quasi-experimental study 24 active men based on their age were divided into two groups including 30-35 (young) and 55-60 years old men (middle-aged). Participants performed upper body exercise on a Monark arm ergometer. During the test, heart rate was recorded continuously by electrocardiogram. Also, participants' heart rate was recorded for 10 minutes after completion of the test. In order to measure the time domain parameters of heart rate variability (PNN50 and RMSSD), one-minute successive RR waves intervals collected in 5 minutes and 10 minutes after the completion of the test and then were transmitted to the heart rate variability software for analysis. Also, heart rate recovery was measured in one minute and two minutes after the test by the difference between the maximum heart rate during the test with heart rate in one and two minutes after the completion of the test.
Results: There was a significant difference between PNN50 in minutes 5 and 10 after the test in young men (P<0.05). Young men also had higher values of RMSSD in minutes 5 and 10 compared to middle-aged men. The heart rate recovery in the 2 minutes after test was significantly higher than 1 minute after test in both groups (P<0.05). Heart rate recovery was higher in young people than in middle-aged people at 5 and 10 minutes after high-intensity exercise due to faster withdrawal of sympathetic nerves.
Conclusion: Changes in the autonomic nervous system is dependent on the type, duration of activity, and the age of the participants.
 

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Background and Objective: Childhood behavioral and emotional problems may be continus to puberty, even extend to elderly. This study was carried out to determine the role of family accommodation of child symptoms, parenting style and parental stress in prediction of anxiety disorder in children with 6 - 10 years old in Golestan province, north of Iran.

Methods: In this descriptive-analytic study 300 children with 6-10 years old and their mothers were selected by randomized cluster sampling method during 2017. The questionnaires of family accommodation scale-anxiety (FAS-A, 2013), parent stress index-sort form (PSI-SF, 2000), Bamerind parenting styles questionnaire 1973 and Spence child anxiety scale-parent form (SCAS-P, 1998) were completed by the mothers.

Results: Increasing family accommodation and parenting stress significantly correlated with increasing children anxiety (P<0.05). The authenticated childbearing style by parents significantly correlated with reducing the anxiety in children (P<0.05), but authoritarian parenting and permissive parenting by parents significantly correlated with increasing the anxiety in children (P<0.05). Authenticated childbearing style along with authoritative parenting style, parental stress and family support could predict 49% of children's anxiety (F=97.91, P<0.0001).

Conclusion: Authoritative and authoritarian parenting, parenting stress, family accommodation has an effective role in predicting children's anxiety.

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Background and Objective: Gingival recession is a common disease leads to esthetic problems, increased sensitivity of teeth and root caries. This study was done to determine the prevalence of gingival recession in dental students with high level of oral hygiene.
Methods: This descriptive-analytic study was performed on 214 dental students of Golestan University of Medical Sciences, Gorgan, Iran during 2017-2018. After collecting demographic information, clinical examination including Oral Hygiene Index (OHI) and Gingival Index (GI), Severity of gingival recession, width of keratinized gingival, attachment of frenulum, toothbrush trauma, tooth malpositions, periodontal diseases and other iatrogenic factors was measured.
Results: Prevalence of gingival recession was 23.8% .Class 1 and 2 of gingival recession was 84.62 % and 15.38%, respectively. Class 3 and 4 of gingival recession did not observe in any subjects. Toothbrush trauma was the most etiologic factor in 35.4% of dental students suffering of gingival recession (P<0.05). Also, dental malposition with 20.83%, loss of keratinized gingival with 14.58%, orthodontics procedures with 12.5%, periodontal diseases with 10.4% were the other factors in gingival recession.
Conclusion: Toothbrush trauma and dental malposition are the most important etiologic factors in dentistry students suffering from gingival recession.

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Background and Objective: Cryptosporidium is one of protozoan parasites. Cryptosporidium is important in human public health. This parasite has many species, some of which are common in animal and human hosts. One of the animal hosts of this parasite is pet bird. This parasite causes digestive and respiratory problems in pet birds. This study was performed for molecular identification of Cryptosporidium as a zoonotic pathogen of pet birds.
Methods: In this descriptive laboratory study, fecal samples of 114 cages (50 Passeriformes and 64 Psittaciforms) from all over Isfahan city in Iran were collected by collecting history and after extracting the genome; the Cryptosporidium was detected by specific primers, based on ssrRNA gene.
Results: In 16.66% of the canaries with gastrointestinal symptoms and 4.54% of apparently healthy canaries the ssrRNA gene of Cryptosporidium was detected. In addition, the gene was detected in 10% of cockatiel and 4.16% of budgerigars and in other species of Psittaciforms was not detected.
Conclusion: Pet birds in Isfahan can be considered as a source of Cryptosporidium infection.

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Background and Objective: Behavioral disorders among children and adolescents are common and incapacitating which cause many problems for the families and the patient themselves and associated with high prevalence of negative social consequences. This study was done to evaluate the prevalence of psychiatric disorders among children and adolescents in Gorgon, north of Iran (2019).
Methods: This descriptive-analytical study was performed on 1025 children and adolescents aged 6-18 years in Gorgan, north of Iran (2019). Subjects were selected by Multistage Cluster Sampling. Digital version of K-SADS test was studied for 24 types of child and adolescent psychiatric disorders. Sampling was done by trained psychologists, among general population and by referring to the house of people. Psychiatric disorders included mood disorders, behavioral disorders, neurodevelopmental disorders, substance abuse disorders, and elimination disorders.
Results: The prevalence of psychiatric disorders among children and adolescents was 13.2%. This prevalence was 14.3 % and 12.1% in males and females, respectively. According to age groups, The prevalence of psychiatric disorders was common in 6 to 9 years olds with 17.8% which in comparison with other age groups was significant (P<0.05). Eliminatory disorder with 6.1% was the most common illness followed by behavior (6%), tension disorders (4.8%) and neurodevelopmental disorders (3.3%).
Conclusion: The prevalence of psychiatric disorders among children and adolescents in this area is in a median range compared to other domestic and overseas.

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Background and Objective: The emergence of drug resistance in Mycobacterium tuberculosis and especially drug resistant strains has created problems for the treatment of tuberculosis control, worldwide. This study was performed to evaluate the changes in N-acetyltransferase (NAT) pattern of methylsonone in patients who did not respond to first-line treatment for Mycobacterium tuberculosis.
Methods: This descriptive-analytical study was performed on 200 patients with tuberculosis. After preparing the blood sample, saturated salt method was used to extract DNA. For the determination of quantity and quality of DNA, two methods of spectrophotometry and electrophoresis on agarose gel were used. The methylation pattern of NAT1 gene was investigated by HRM method.
Results: 34 patients were resistant to the first line of treatment. 18 patients showed hypermethylation pattern, 12 patients showed non-methylated pattern and 4 patients showed hypomethylated pattern. 166 patients were not resistant to the first line, of which 23 patients in hypermethylated group, 120 patients in group Non-methylated and 23 patients were in the hypomethylated row. There was a statistically significant relationship between methylation level and drug resistance (P<0.05).
Conclusion: This study showed that resistance to first-line treatment of Mycobacterium tuberculosis was related to the level of N-acetyltransferase gene methylation.

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Background and Objective: The use of packaged drinking water is on the rise nowadays in most countries, including Iran. Currently, more than 100 different brands of packaged drinking water are produced and distributed in Iran. This study was done to evaluate the quality of Iranian, foreign packaged drinking waters and municipal drinking water in Golestan Province, north of Iran.
Methods: This descriptive-analytical study was done on 56 packaged drinking waters of different Iranian and foreign brands, eight brands produced in Golestan province and a number of municipal drinking water samples were collected from Gorgan and Gonbad Kavous cities in northern Iran. To assess the quality of packaged drinking water and drinking water, their physicochemical parameters were compared with National Iranian Standards 1053 and WHO. Stiff and Piper diagrams were plotted to determine the type and hydrochemical facies of water samples. Gibbs and Schoeller diagrams were used to determine the water chemistry controlling factors of water samples and their water quality for drinking, respectively.
Results: The concentrations of physicochemical parameters (except bicarbonate) were within the range of national drinking water standards 1053 and WHO. The average nitrate concentration in all packaged drinking and drinking water was within the standard range. The fluoride concentration of all packaged drinking waters and drinking water was within the range of WHO standard. However, only 14 samples of all packaged drinking and drinking water samples are within the range of 1053 National Iranian Water Standard. The total concentration of soluble solids and the total hardness of packaged drinking water were within the range of 1053 NW. There was also a significant difference (P<0.05) between the chemical parameters of magnesium, sodium, chloride and nitrate in packaged drinking water produced in Golestan province with the municipal drinking water samples.
Conclusion: The amount of fluoride in drinking water in this study was less than desirable and required fluorination. The quality of packaged drinking water in Golestan province is better than the municipal drinking water.

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Background and Objective: The acute exercise leads to the induction of some cardiac and inflammatory biomarkers that are related to factors such as severity, type, training experience, gender and environment. This study was conducted to compare the effect of acute endurance and resistance training at two different intensity levels of high-sensitivity cardiac Troponin T (hs-cTnT) and tumor necrosis factor alpha (TNF-α) in male runners and bodybuilders.
Methods: In this quasi-experimental study, 20 male runners and bodybuilders with an age range of 20-24 years were purposefully and accessibly selected. The study was performed with a pre-test-post-test design on 10 male runners (endurance training) and 10 bodybuilding men (resistance training). The runners ran 6 km with moderate (70-75% of reserve heart rate) and high (85-85% of reserve heart rate) intensities at intervals of one week, during separate sessions. Bodybuilders also performed resistance training at moderate (70-75% of one repetition maximum) and high (80-85% of one repetition maximum) intensities.  Bodybuilders also performed resistance training at moderate (70 to 75% of a maximum repetition) and high (80 to 85% of a maximum repetition) intensities. Blood samples were taken before and immediately after exercise. Then the serum levels of hs-cTnT and TNF-α were measured.
Results: The serum level of hs-cTnT and TNF-α was significantly increased after acute running and resistance training with moderate and high intensities (P˂0.05), but intense acute exercise had a greater effect on increasing the levels of these variables. Also, the acute effect of intense aerobic exercise was associated with a greater increase in hs-cTnT level in compared to moderate-intensity aerobic exercise, high and moderate-intensity resistance training (P˂0.05).
Conclusion: In endurance and resistance athletes, the hs-cTnT and TNF-α responses are affected by the intensity of training and increase more with intense training. But the high intensity aerobic exercise is associated with a greater increase in hs-cTnT levels.