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Background & Objective: Enuresis is a problem in children. In this research the success, rate and recurrence of Imipramine, Desmopresine and conditional therapy has been studied in enuresis treatment of children. Materials & Methods: This study was done on 84 enuresis child between 7-11 years old which were selected by screening at the schools. Patients divided randomly to 3 groups: The 1st group received 25 mg of Imipramine every night. The 2nd group was treated with 20 micg of Desmopresine intranasaly every night. The 3rd group used alarm for conditioning every night. All groups were treated for one month, and 2 weeks without enuresis meaned success. Results: All physical finding and laboratory tests were normal in all patients (FBS, U/A, U/C, kidney and bladder sonography). Patients had in average 8.5 years old. 77.3% of cases were boys and 22.7% were girls. Success rate for therapy was 71.4%, 60% and 50% in-groups 1-3 respectively. Recurrence rate 6 months after therapy was 10%, 55.5% and 25% in-groups 1-3 respectively. Conclusion: Imipramine persists as cheep, available, well-tolerated and effective way of treating enuretic patients. Due to high recurrence rate, Desmopresine is just recommended as a temporary treatment.

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Background&Objective: Foodstuffs additives are a general term for materials that are added to the foodstuffs in order to increase durability and to improve the appearance, composition, taste and food nutritional value. Colors are classified in this group and are added to foodstuffs in order to increase their attraction. Colors may cause illness such as allergy, rash and hyper activation in children and also they may debilitate the Immune system, Anaphylaxis reactions may also occur and they may have cancerous effect. The aim of this survey was to analyze status of added colors to the dried sweets which are produced in south of Tehran city. Materials & Methods: 191 samples of dried sweets were randomly collected and analyzed from south of Tehran areas. First, the samples were de-colored by Clorhidric Acid, and then were analyzed after refining by Thin Layer Chromatography (T.L.C) method. Samples were identified by taking Retention Factor (RF values) into consideration. Results: 93.2 percent from the total samples contained colors. Among chromatic samples, 42 samples (22%) out the total samples, contained artificial, non-edible colors and 96 samples (50.3%) from the total samples contained artificial and edible colors (for Industrial Producers) and 40 samples (21%), contained natural colors. Sunset yellow color was detected more than other added colors in sweets. Conclusion: Low costs, stability, PH, purity, and environmental conditions, motivate the producers for high utilization of edible colors without considering their possible hazards and/or their edible quality aspects. It is suggested that, based on the findings of this study and high consumption of colors.

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Background and Objective: In recent years, chitosan and Moringa oleifera Coagulant Protein a natural coagulant were used in order to reduce the problems occure from chemical coagulants. This investigation was done to determine effectiveness of Moringa Oleifera Coagulant Protein and Chitosan as natural coagulant aids in removal of colloidal particles and bacteria from turbid waters. Materials and Methods: In this interventional- quasi experimental study, the experiments were run by using synthetic water having low(10-20NTU), medium(100-120NTU) and high (200-220NTU) initial turbidities. In order to determine optimum pH and dosage of coagulant and coagulant aids, a conventional jar test apparatus was employed. Turbidity reading were carried out using a Hach model 2100P Turbidimeter. The samples were taken from the top four inch of the suspension for turbidity and bacteria removal measurement. Results: Optimum dose of alum for waters with three different initial turbidities were 20, 40, and 20 mg/l, respectively. Optimum pH was between 7-7.5. Moringa oleifera Coagulant Protein and chitosan were reduced the required dosage of alum from 12.5% to 62.5% and from 50% to 87.5%, respectively, in different turbidities and residual aluminum was reduced to standard limit (0.2mg/l). The bacteria removal efficiency were from 90% to 99.9999%. It was found when samples were stored during 24 hours regrowth of E.coli was not observed. Conclusion: This study showed that natural coagulant aids can reduce the turbidity to below 5NTU without filtration in optimum condition.

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Background and Objective: Asthma is the most prevalent chronic disease during childhood and it is estimated that 4.8 million children involved in asthma all over the world and its prevalence and incidence is increasing. The aim of this study was to identify the peak-flow meter application on quality of life in asthmatic children. Materials and Methods: This clinical trial study was done on 80 asthmatic children between 7 to 15 years old which referred to Shahid Beheshti hospital clinic in Kashan, Iran during 2009. Susan Sylvia questionnaire included 3 parts, was filled for every child. The questions in any section had three options in which there were 5 items in activity limitation, 13 questions in symptoms and 6 questions in emotional functions. Every question had 1 to 3 points and whole questionnaire taken 23 to 69 points. The children were learnt to measure their PEFR (Peak Expiratory Flow Rate) 3 times a day using Peak flow meter device and upon the results, the management procedure was changed. After 3 months the outcome were evaluated and data analyzed by SPSS-15 and paired t-test. Results: Mean age of patients was 8.82±2.75 years old. Thirty three subjects (41.3%) were girl. The average score in emotional functions was 13.1±3.07 before and 14.35±3.7 after using peak-flow meter (P<0.05). The average score in activity limitation was 7.33±1.57 and 8.47±0.67 before and after using peak-flow meter, respectively (P<0.05). The average score in symptoms before and after pek-flow meter was 28.81±5.13 and 32.02±5.62, respectively (P<0.05). Finally total score of quality of life showed significant increase in children after using peak-flow meter (P<0.05). Conclusion: This study showed that the application of pek-flow meter increase emotional functions, decrease limitation of activity and symptoms in asthmatic children. Therefore, it is suggested pek-flow meter dievice taking into account in asthma management of asthmatic patients particularly in children.

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Background and Objective: Fungi are widely distributed in nature and they are usually present in attomospher but other sources such as water play an important role in their ecology. This study was done to evaluate mycoflora assessment in drinking tap water in Sari, North of Iran. The tap water collected form Sari water distribution system for fungi. Materials and Methods: In this descriptive study, a volume of 100 ml of tap drinking water samples (n=60) were collected in sterile bottles. All water samples passed through sterile 0.45 micrometer filters. The filters were placed directly on Malt extract agar and incubated at 27°C for 3-7 days. Routine mycological techniques were applied to identify the grown fungi. Results: Out of 468 grown fungal colonies, eight different fungal genera were identified. The total mean cfu per 100 ml for the positive samples were 8.4. Aspergillus (37.4%) and Penicillium (27.3%) were the most common isolated fungi. Rhizopus (0.6%) had the lowest frequency. Among Aspergillus species, A. flavus had the highest frequency. Conclusion: Our result showed that various fungi were present in the tap drinking water. We propose fungi should be considered as part of the microbiological analysis parameters in drinking tap water.

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Background and Objective: Probiotc bacteria have benefical effect on consumer health. This study was done to investigate the antimicrobial effect of several probiotic in combinations with different prebiotics against food patoghenic bacteria including Staphylococcus aureus and Listeria monocytogenes.

Methods: In this descriptive - analytical study, probiotics including Lactobacillus plantarum, L. acidophilus, L. fermntum, L. casei and L. rhamnosus with prebiotics (1%) including raffinose, lactulose, inulin and trehalose were cultured in MRS broth for 24 hours at 30ºC in anaerobic conditions. Antimicrobial property of them was determined with well diffusion plate's method.

Results: Probiotics in the presence of prebiotics indicated the higher antimicrobial effect compared to probiotics alone (P<0.05). The application of prebiotics such as L. casei with raffinose showed higher antimicrobial property against Listeria monocytogenes than the free prebiotics consumption. The diameter of inhibitory growth zone in the presence of raffinose as a prebiotics was 14.66 mm and its absence reduced to 11.75 mm.

Conclusion: Antimicrobial effect of probiotics in combination with prebiotics against Staphylococcus aureus and Listeria monocytogenes was higher than probiotics consumption alone.

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Background and Objective: Salmonellosis is a gastroentritidis which caused by the different serovars of Salmonella genus, and responsible for morbidity and mortality worldwide. Food born disease is one of the growing problems of human societies especially in developing countries. The aim of this study was to evaluate and serogroup determination of Salmonella isolates from food along with antibiotic resistance pattern.
Methods: This descriptive study was performed on total of 400 in equal of 200 packed and 200 unpacked  samples of (red meat, chicken meat, egg, vegetable) collected in random from distributed in Tehran ,Iran during nine months in 2018. Microbial, biochemical and serological test was performed according to protocol number of 1800 of national standard. Antimicrobial susceptibility test was done by disk diffusion (MAST, Co, UK) method.
Results: Out of 400 samples 8 (2%) was identified as Salmonella. The unpacked foods were more contaminated (75%) compared to packed foods (25%). The most isolated serogrouping were belonging to especially D. Salmonella. The chicken samples were more contaminated (37.5%) than other samples. The isolated Salmonella were mostly resistance to nalidixic acid (75%).
Conclusion: The Salmonella isolated particularly from group 1 showed higher antimicrobial resistance, additional care should be taken in preparation, packaging and supplying the food samples.

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Background and Objective: Yersinia is water and foodborne organism that cause human gastroenteritis. This study was done to evaluate the frequency of Yersinia species isolated from children diarrheal samples and chicken meat in Tehran, Iran.
Methods: In this descriptive study 250 sample of diarrhea of children referred to the Children's Medical Center, Tehran, Iran and 250 samples of chicken were collected and examined for Yersinia infection during July 2016 to March 2017. Isolation method was performed based on initial enrichment in phosphate buffer for 3 weeks in refrigerator (cooling in c4 +) and then using KOH as secondary enrichment and culture on CIN agar medium. Biotyping method was used to determine pathogenic strains.
Results: In this study, 5(2%) isolates from pediatric diarrhea samples and 20 isolates (8%) from chicken meat samples were obtained from Yersiniaenterocolitica. Biotyping of human Yersiniaenterocolitica isolates identified 3 cases of biotype 1A, one case of biotype 1B, one case of biotype 2 and from chicken meat isolates, 16 isolates belonged to biotype 1A and 4 isolates belonged to biotype 1B.
Conclusion: Presence of common pathogenic 1B and non-pathogenic 1A biotypes in pediatric diarrhea samples and chicken meat can indicate the cause of diarrhea in children.