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Background and Objective: Ultraviolet (UV) radiation is a important disinfectant. Fungal infections with resistant isolates in patients culminate in recurrence of disease even with worse condition. This study was done to evaluate the efficacy of ultraviolet radiation on drug susceptibility of Candida Spp. to itraconazole, fluconazole and amphotericin B. Materials and Methods: This laboratory study was done on 12 Candida spp. isolated from patients according to NCCLS M27- A method. Samples were suspended with sterile saline and optical density was read by spectrophotometer at the wavelength of 530 nm. Serial dilutions (0.0313-16 µg/ml) and (0.0313-128 µg/ml) were supplied for itraconazole, amphotericin and fluconazole, respectively. MICs were determined after 48h incubation at 35°C. Following UV radiation for 1, 2, 5, 10, 60, 90 and 120 seconds MICs were determined, subsequently. Results: The highest MIC pre UV radiation was (>128 µg/ml) for fluconazole. After UV radiation, MICs were steadily decreased for all mentioned drugs while after 10 sec, MICs of itraconazole and amphotericin B were >0.0313 µg/ml. Secondary MICs significantly decreased with respect to MICs obtained in pre UV radiation (P<0.05). Conclusion: UV radiation reduces MICs of Candida spp. to itraconazole, fluconazole, amphotericin B.

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Background and Objective: Aspergillosis is the most current causative agent of exogenous fungal nosocomial infection. This study was done to evaluate the drug susceptibility of Aspergillus flavus and A.fumigatus to itraconazole and amphotericin B. Materials and Methods: This Laboratory study was done on 25 Aspergillus fumigatus and 25 Aspergillus flavus species isolated from transplant's patients. Drug susceptibility test was done according to NCCLS M38-P document. Fungal suspensions of mentioned fungi were supplied with ranges 0.5–5×10⁴ by spectrophotometer at 530 nm. Serial dilutions of drugs were supplied from 0.03125 to 16 µg/ml and MICs determined following 48h incubation at 35°C. Results: Obtained MICs ranges for Aspergillus fumigatus and Aspergillus flavus were 1-4 µg/ml and 0.5–4 µg/ml for itraconazole, respectively while MICs ranges against Aspergillus fumigatus and Aspergillus flavus were 0.5-2 µg/ml and 0.25-2 µg/ml for amphotericin B, respectively. Amphotericin B MICs were significantly lower than itraconazole (P<0.05). Conclusion: Aspergillus flavus and A.fumigatus were susceptible to amphotericin B and itraconazole.