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The assessment to bacterial epidemiology in large hospitals help the management of the febrile patients and prescribing the correct kind of antibiotics, before receiving the laboratory results. In a descriptive study during (1996-97) in different hospital departments. The samples were transferred to the laboratory and common bacterias were isolated. In this study, cultural mediums of stool, blood, wound, were used as source the samples the results indicate that, the most common bacteria was E.coli with 30.6%, Staphylococci 14.2% and common infection sites were, urinary 33.3%, blood 21.7% respiratory tracts 14% respectively. The results from this investigation shows that frequency and distribution pattern agrees with those reported by other researchers. The susceptibility of isolated bacteria to Ciprofloxaxin and amino glycosides were quiet high.

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Background and Objective: Self-injury (SI), also called self-mutilation or cutting, is highly stigmatized emotional disorder. The aim of this study was to estimate the incidence rate of self-injury among male pupils in Tabriz – North West in Iran and to evaluate the associated risk factors. Materials and Methods: In this longitudinal study of all the pupils high school in Tabriz, 1772 (13.7%) were randomly sampled, and assessed in this and subsequent year. A self-administered questionnaire with 48 questions was distributed to high school in February and March 2005. After one year (February and March 2006), another questionnaire with 10-items was distributed to those students to determine the incidence rate of self-injury. The influence of different factors on self-injury was evaluated with a logistic regression model. Results: The mean age of the subjects was 16.3±0.87 (min 15, max 19) years. At the end of follow–up, 4.8% of pupils reported incidence of self-injury. Most type of incidenced self-injury were cutting and burning. The results of logistic regression model indicate that transition in smoking stages (OR=2.81), incidence of alcohol drinking (OR=2.27) and having smoker friend (OR=1.78) were factors associated with incidence of self-injury. Conclusion: This study showed that incidence rate of self-injury in this group of adolescents was 4.8% in one year, which related to age, alcohol and ciggaret smoking.

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Background and Objective: There are some factors including socio-demographic, maternal and nutritional variables reported to influence the growth of children in the first two years of life. The aim of this study was to determine some factors related to undesirable growth of 6 months–2years old children in Tabriz-Iran. Materials and Methods: In this case - control study 233 children with undesirable growth as cases, and 412 children with desirable growth as controls selected in five primary health care centers in Tabriz city in North-West of Iran during 2007. A questionnaire which included some questions regarding demographic characteristics, fertility history, and nutritional status was filled by using face -to - face interview with selected children’s mother. The influence of different factors on undesirable growth was evaluated with a logistic regression model by using SPSS-14 and EPI-2000 software. Results: Having any disease during two monitoring (OR=4.23, 95% CI: 2.95-6.07, P<0.001), type of pregnancy (unwanted) (OR=1.87, 95% CI: 1.20-2.93, P<0.006), gender (girl) (OR=1.51, 95%CI: 1.05-2.17, P<0.025), low birth weight (OR=1.0, 95% CI: 0.99-1.01, P<0.097) and having conflict in the family (OR=1.22, 95% CI: 1.01-1.47, P<0.045) associated with undesirable growth. Conclusion: This study determined having any disease, type of pregnancy, sex of child and low birth weight related with undesirable growth of 6 months –2years old children. Using safe family planning methods and help to reduce unwanted pregnancy, parental training about the nutritional requirements of children particularly during period of disease, is recommended in order to enhance the health of children.

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Background and Objective: Tinea versicolor is a common fungal infection of the skin caused by the dimorphic lipophilic yeast Pityrosporum orbicular (Malassezia furfur). Lesions begin as multiple small, circular macules of various colors. The upper trunk is most commonly affected due to the side effects of oral treatment and drug resistance, this study was done to compare the therapeutic efficacy of topical terbinafine versus topical ketoconazole in Tinea versicolor. Materials and Methods: This Randomized double blind clinical trial study was conducted between 2008-09. Sixty nine patients with a clinical diagnosis of pityriasis versicolor confirmed by microscopic potassium hydroxide (KOH) examination were taken for the study. Patients randomly divided into 2 treatment groups: 35 patients in terbinafine group, treated by terbinafine 1% once daily for 2 weeks and 34 patients in ketoconazole group, treated with ketoconazole 2% once daily for 2 weeks. Five patients of terbinafine and 4 patients of ketoconazole groups were excluded due to lack of follow-up. Patients were followed up at monthly intervals for 3 months and recurrence and cure rate for each subject were recorded. Data was analyzed by SPSS-16, t student and Chi-Square test. Results: The mean moderate cure rate obtained one month after treatment was 20% in terbinafine group versus 3.3% in ketoconazole group, and there was no any significant difference between two groups. The mean moderate cure rate two month after treatment was 67.7% in terbinafine group and 60% in ketoconazole group (P<0.05). The mean complete cure rate three month after treatment was 73.3% in terbinafine group and 10% in ketoconazole group (P<0.05). The percent of positive KOH examination, three month after treatment was 10% in terbinafine group and 36.7% in ketoconazole group (P<0.05). Conclusion: This study showed that terbinafin is more effective than ketoconazole in treatment of tinea versicolor.

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Background and Objective: Several strains of the Echinococcus granulosus have been described based on morphological characters, intermediate host specificity and/or genetic analysis of mitochondrial and nuclear DNA. The aim of this study was to characterize different E.granulosus isolates by using sequences of mitochondrial atp6 gene.

Materials and Methods: In this study, Sixty infected liver and lungs of cattle, sheep and goats were collected from the abattoir of Varamin city-Iran during 2008. Protoscoleces were removed from each fertile cyst and DNA extracted. New and specific primers were designed for two existing genotypes (G1 and G6) of E. granulosus known to occur in Iran and applied in PCR reactions.

Results: The new primers selectively amplified the G1 and G6 genotypes of E. granulosus with specific bands of 708 and 705 bp respectively. The G1 genotype was identified in all fertile cyst samples.

Conclusion: This study showed that the new primer pairs which specifically amplify portions of the mitochondrial atp6 gene of the G1 and G6 strains of Echinococcus granulosus are proper molecular marker for investigating genetic variation in a number of isolates of E. granulosus from a range of hosts (sheep, goats, cattle) in Iran. The result of sequenced samples showed that our sequences were the same as those reported previously for these strains.

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Background and Objective: Normal psychological behaviour is one of the general concept of health. This study was carried out to evalute psychological disorders among inhabint residing in poor social district of Gorgan, Iran. Materials and Methods: This cross-sectional study was done on 376 subjects (188 males and 188 females) with simple random sampling method. Data were gathered using the general health questionnaire (GHQ-28). Data was analyzed by SPSS-16, Kruskal–Wallis and Pearson tests. Results: The prevalence of psychological disorders was 37% (33.5% in men, 40.4% in women), depression was 24.45% (22.5% in men and 26.4% in women) and anxiety was 31.6% (31.2% in men, 32% in women). Social affairs disorder 21% (19.2% in men, 22.7% in women) and psychosomatic disorders in 28.5% (22% of men, 35% of women). The relationship between psychological disorder with economic level and family size was significant (P<0.05). Conclusion: This study showed that 37% of inhabint residing in poor social district of Gorgan, Northern Iran had psychological disorders.

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Background and Objective: Improving the ability to walk is often a key target for the treatment of abnormal gait in children with spastic diplegic cerebral palsy (CP). One of the goals of orthotic rehabilitation is to improve walking in this field. The aims of this study was to design and manufacture the dynamic neoprene orthoses and evaluate its impact on the gait parameters in children with cerebral palsy. Materials and Methods: This quasi-experimental study was done on 12 children with spastic CP at University of Social Welfare and Rehabilitation, in Tehran, Iran during 2010-11. Initially neoprene dynamic orthosis is designed specifithy for each subject, this neoprene dynamic orthosis was used for six weeks and 6-8 hrs daily. For evaluating the walking speed and the gait variation, 10 meter walking test and visual analogue scale have been used. Modified Ashworth’s Scale and electro-goniometre were used to assess muscle spasticity and the flexion degrees of knee joint. Data were analyzed using SPSS-16, Kolmogorov-Smirnov and Paired t-tests. Results: The alteration of knee flexion angle, walking speed and walking distance following dynamic orthosis were -18.31±4.61 (degree), -0.50±1.82 (meter) and 4.18±1.51, respectively. The improvement in knee joint angle and walking following dynamic orthosis was significant (P<0.05), but the walking speed was not significant. Conclusion: This study showed that neoprene dynamic orthosis can improve knee flexion angle and walking distance among children with spastic diplegic cerebral palsy.

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Background and Objective: The risk of coronary heart disease (CHD) is proportional to the LDL-C lipoprotein. Due to frequent use of Friedwald formula in estimation of LDL-C in most laboratories, this study was done to compare the Friedwald formula and direct measurement to determine the serum levels of LDL-C Materials and Methods: This descriptive study was conducted on of 598 patients 226 male and 372 female whome referred to Imam Ali hospital Andimeshk cityin Khozestan province of Iran for health check up during 2009. 5 ml of the venous blood was drown. Total cholesterol (TC) (mg/dl), Triglyceride (TG) (mg/dl), HDL-C (mg/dl) and LDL-C (mg/dl) of serum are measured with Pars azmun company kits. The Friedwald formula was used for estimation of LDL-C. The K=3, 3.5 and 5 were used to stimate the lipid by Friedwald formula. Data were analyzed using SPSS-18, Pearson correlation coefficient and ANOVA tests. Results: A total of 598 serum samples collected 37.8% were men and 62.2% women. The mean age of participants was 38.8±10.77 years. Minimum age 21 years and maximum age was 77 years. Mean deviation for TG≤150, 201-300 and 301-400 in Friedwald formula (k=5) were -13.01±8.79, -17.11±13.17 and -18.63±18.54, respectively and with k=3 are -.39±12.04, -0.078±18.55 and 0.04±25.55 and for TG between 151-200 is -9.72±10.54 and with k=3.5 is equal to 0.82±13.70. Pearson correlation test showed that direct measurment and calculated from the equation Friedwald, for triglycerides in the area equal to or less than 150, 151-200, 201-300 and 301-400 mg/dl, with correlated to Pearson correlation coefficient were 0.982, 0.991, 0.991 and 0.975, respectively. Conclusion: This study showed that the direct measurement method is superior to the Friedwald equation, otherwise, equation Friedwald formula with K=3 is recommended.

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Background and Objective: Nitric oxide synthase (NOS) paly a role in nitric oxide (NO) generation. Despite the beneficial effects of NO on different body systems its overproduction of produce reactive nitrogen species (RNS) and nitrosilation of proteins. This study was done to evaluate the effect of asymmetric dimethylarginine (ADMA) and NG-Monomethyl-L-arginine methyl ester (L-NMMA) on inhibition of nitric oxide synthase activity. Materials and Methods: In this laboratory study, Nitric oxide synthase was extracted from 500 grams of sheep kidney by homogenization, ammonium sulphate precipitation and column chromatography on DEAE-32 Cellulose and 2', 5'-ADP-agarose. During purification, protein content was measured according to the Bradford and enzyme activity was assayed using the Griess reactions the inhibitory effects of 25 μΜ concentrations of ADMA and L-NMMA on purified enzyme were determined. Results: Specific activity and yield of NOS were 0.6 units/mg protein and 0.9%, respectively. Molecular weight of purified enzyme was 54 KD with SDS-PAGE. ADMA and L-NMMA in 25 μΜ concentrations reduced enzyme activity by 76 and 61.2%, respectively. Km values for NOS in absence and in presence of ADMA and L-NMMA were 5.32 μM, 31.25 μM (P<0.05) and 14.29 μM (P<0.05), respectively. Vmax for NOS in absence and presence of inhibitors was not changed. Conclusion: ADMA and L-NMMA have competitive inhibitory effect on NOS activity and ADMA have higher inhibitory effect than L-NMMA.

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Background and Objective: Several studies reported the immunological activity of Lycium barbarum. This study carried out to determine the effect of aqueous extract of fruits of Lycium ruthenicum L. on morphometric and histomorphometric indices in mice lymphatic organs. Methods: In this experimental study, 36 adult mice were randomly allocated into six experimental and control groups. The experimental groups were received Lycium ruthenicum L. fruit aqueous extract in the doses of 50, 100, 200, 400 and 800 mg/kg/bw, daily by feeding tube for 21 days. In the control group animals were recived 100 mg/kg/bw of saline using feeding tube. After the treatment, the relative increase in the body weight, morphometric, histomorphometric and histopathologic indices in thymus, lymph node and spleen were measured. Results: The administration of extract in doses of 50 and 100 mg/kg/bw did not effect on body weight of mice but in the doses of 200, 400 and 800 mg/kg/bw significantly reduced the body weight (P<0.05).Thymus index in 800 mg/kg/bw of extract significantly reduced in comparison with controls (P<0.05). Thickness of thymus medulla in low doses significantly increased while it was not visible in higher doses (P<0.05). Thickness of capsule in lymph node and spleen in dose of 800 mg/kg/bw significantly increased in comparison with control group (P<0.05). Low doses of the Lycium barbarum extract increased stimulation and infiltration of the immune cells and hematopoiesis in the lymphoid organs while in doses of 400 and 800 mg/kg/bw caused pathological changes including fibrosis in capsule, degeneration in follicles and stromal cell apoptosis. Conclusion: Aqueous extract of fruits of Lycium ruthenicum L. in doses of 400 and 800 mg/kg/bw causes histopathological alterations in the lymphoid organs.

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Background and Objective: Several methods are available for measuring fever in children. This study was done to compare the accuracy of three method of measuring body temperature using left and right tympanic, axillary and rectal methods in three months to five years old children.

Methods: This descriptive-analytic study was done on 126 children (63 without fever and 63 children with fever) with 3 months to 5 years age in Mofid hospital, Tehran, Iran. Rectal temperature lower than 38°C was considered as Gold standard to determine fever. Body temperature was also recorded for subjects through right and left tympanic and axilary methods.

Results: Body temperature was recorded in axillary method 37.1°C (sensitivity: 92.1%, specificity: 90.5%), right tympanic 36.9°C (sensitivity: 74.6%, specificity: 84.13%) and left tympanic 37.3°C (sensitivity: 93.65%, specificity: 84.13%). The mean temperature in the axillary method 0.77°C, right tympanic 1.02°C and left tympanic 0.48°C was lower than the mean rectal method (P<0.05). The correlation between right, left tympanic and axillary with rectal method was 0.84, 0.894 and 0.925, respectively (P<0.05). The area under the receiver operating characteristic (ROC) curve for left and right tympanic and axillary were 0.95, 0.87, 0.965, respectively.

Conclusion: The difference between rectal and left tympanic method was at the lowest level due to the ease of measuring temperature through tympanic membrane in three months to five years old children.

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Background and Objective: Breast cancer is one of the most common types of cancer among women. HER-2 molecule as the receptor of tyrosine kinase from the family of epithermal growth factor is a major cause of cancer. The Herceptin protein molecule, which is an anti-HER-2 antibody, can play an important role in the diagnosis and treatment of breast cancer. This study was done to subcloning of Herceptin gene, expression in the prokaryotic system (E.coli) and produce Herceptin recombinant protein for use in the treatment of breast cancer.

Methods: In this descriptive – laboratory study, Herceptin gene from synthesized construct was isolated by enzyme digestion, and then subcloned to the expression vector pET28a. Subcloning of the gene was confirmed using PCR and enzyme digestion. After transferring the vector into E.coli BL21 DE3, expression of the recombinant Herceptin gene was induced by IPTG. The recombinant protein was evaluated by SDS-PAGE and purified with Ni-NTA column chromatography and finally verified by western blotting using anti-histidine antibody. The survival of cells adjacent to recombinant Hercaptin by MTT was investigated.

Results: Following the subcloning of the Herceptin gene, PCR and enzyme digestion, the 741 fragment of the Herceptin gene was confirmed. Confirmation of Herceptin's recombinant protein and its evaluation on SDS-PAGE gel about 27 kDa was done. The recombinant protein was also confirmed with anti-histidine tag. The purified protein adjacent to the SKBR3 cell line was able to block the growth of cancer cells.

Conclusion: Regarding the expersion of HER2 antigen on surface of breast cancer cells, Herceptin can act as antibody blocker and it arrests the growth of breast cancer cells.

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Background and Objective: Congenital anomalies are one of the major causes of neonatal mortality. Congenital heart disease (CHD) is the most common birth defect world wide, and recent studies about CHD have reported congenital heart disease approximately is about one percent of all live births. This study was done to determine the frequency of congenital heart disease using ecocardiography in patients with extra-cardiac anomalies in Gorgan peadiatric center in north of Iran.
Methods: In this descriptive study, 55 girls, 84 boys a total of 139 patients were in the age range of birth to 12 years of age whom have referred to Taleghani Teaching and Medical Center in Gorgan during 2012-17. 139 cases of congenital heart disorders with echocardiographic report sheet in their medical record were evaluated. Information of patients including age, sex, ethnicity, type of extra-cardiac, anomalies, cardiac anomalies (simple, complex and ductal anomalies), cardiac murmur status, and clinical symptoms were extracted from their medical records.
Results: From of total of echocardiography, 139 patients (88.5%) were affected by congenital heart disease. The anomalies were included of 88 cases (56.05%), 48 cases (31.21%), 2 cases (1.27%) simple, complex anomaly and ductal dependent anomaly respectively. The other disorders accompanied with congenital heart diseases were 17 cases (12.2%) with cleft palate, 4 cases (2.9%) with congenital diaphragmatic hernia, 8 cases (5.8%) with closed anus, 52 cases (37.4%) with Down syndrome, 4 cases (2.9%) with Turner syndrome, 4 cases (2.9%) with ileum atresia, 11 cases (7.9%) with Esophageal atresia, 7 cases (5%) with Hirschsprung's disease, 6 cases (4.3%) with urogenital anomalies and 26 cases (18.7%) with other extra-cardiac abnormalities.
Conclusion: Down syndrome and cleft palate are among the highest prevalant anomalies with congenital heart diseases.

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Background and Objective: Previous studies have advanced our understanding of the impact of executive functions on eating behavior. Given the considerable evidence of executive dysfunction among obese individuals and the bidirectional relationship between cognition and obesity, it is crucial to include educational components in interventions aimed at enhancing executive functions among obese populations. This study aimed to evaluate the effectiveness of cognitive hypnotherapy in improving executive functions among women with obesity.
Methods: This clinical trial enrolled 30 obese women who were randomly assigned to either an intervention or control group (n=15). The mean ages of the experimental and control groups, respectively, were 40.80±6.02 and 38.47±7.50 years, while their BMIs (kg/m2) were 33.45±3.50 and 33.12±3.47, and hypnotizability scores were 39.67±5.98 and 37.40±7.09. The intervention group received 12 sessions of 2-hour cognitive hypnotherapy. Data collection instruments included the Stanford Hypnosis Susceptibility Scale (1962), Life Experiences, Interests and Values Checklist (1998), and Cognitive Ability Questionnaire (2013).
Results: The results demonstrated that cognitive hypnotherapy significantly improved executive functions (F=7.02, P≤0.01), including inhibitory control and selective attention (F=2.83, P≤0.05), decision making (F=4.13, P≤0.05), planning (F=3.32, P≤0.05), and sustained attention (F=2.83, P≤0.05) in the intervention group.
Conclusion: Given the relationship between cognitive abilities, eating behaviors, and obesity, and the effectiveness of cognitive hypnotherapy in enhancing executive functions, this approach can be used to enhance cognitive functions among individuals with obesity.