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Background and Objective: Breast cancer is one of the most frequent malignancies among women. This study was done to determine the BRCA1 gene expression in 7,12-Dimethylbenz(a)anthracene (DMBA) induced breast cancer in rats. Materials and Methods: In this experimental study, the breast cancer was induced by DMBA in Sprague dawley rats. After tumors arise, cell cultures were prepared and G-banding staining was performed on metaphase chromosomal smear. According to databases, genes in the affected area were collected and after comparing genome of the rats and human in changed chromosomal segments, a gene list was prepared. FISH technique was performed on BRCA1 gene to prove accuracy of chromosomal banding results. Results: Structural changes such as deletion occurred in chromosomes 10, which BRCA1 is located on. 24.7% of cells showed evidence of physical deletion in both copy of BRCA1 gene and 23.8% of cells showed deletion in one copy. Conclusion: Induced DMBA Breast cancer cells showed deletion in BRCA1 copy numbers. This gene may be involved in animal breast tumor model.

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Background and Objective: Coronary artery diseases are one of the main causes of mortality and morbidity. This study was done to determine the prevalence of risk factors for coronary artery diseases in patients referred to angiography center in Sari, Northern Iran. Materials and Methods: This descriptive study was performed on 2540 patients referred to angiography center in Sari city, northern Iran, during 2005-10. Risk factors including diabetes, hypertension, hyperlipidemia, smoking, Family history, CRP and also ECG changes, type and number of involved artery and the severity of vascular involvement were recorded for each patient. Results: Hypertension, hyperlipidemia, diabetes, smoking were seen in 40.8%, 40.5%, 29.1%, 12.5% of patients, respectively. Positive family history and CRP were found in 10% and 3.4% of the patients, respectively. 23.8% of patients had abnormal ECG. One, two and three vessel diseases were observed in 26.5%, 48.3%, 48.3% of cases, respectively. Left anterior descending artery constriction was seen in 75.8% and 80.4% of males and females, respectively. A significant relationship was found between diabetes and left coronary circumflex (P<0.04) and left anterior descending artery (P<0.03) constriction. Conclusion: Diabetes, hypertension, hyperlipidemia, smoking, family history, abnormal ECG are among the risk factors of coronary artery diseases in this area.