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Toxoplasmosis is a disease caused by a ubiquitous, obligate intra cellular protozoan Toxoplasma Gondii. Based on serological evidences, Toxoplasmosis infection is a widespread disease with regard to this fact and because to the occurrence of congenital Toxoplasmosis infections due to asymptomatic infections in pregnant women, determination of the rate of Toxoplasma antibodies (IgG, IgM) before pregnancy and finding the involved factors in increasing Toxoplasmosis prevalence seems to be necessary. This study was a cross-sectional and descriptive study and sample population was the women referred to marriage consultation the prevalence of Toxoplasma antibodies (IgG, IgM) was determined by ELISA method. Blood sample were collected randomly from 300 women referred to the center for consultation in 2001 and they was transferred to the laboratory for antibodies determination. The results showed that the general prevalence to the positive cases based on high titer of IgG was 48.3% and of IgM was 11.7%. There was not any relation between positive cases and age, education, place for residence, job, keeping domestic animals (Except cat) vegetable consumption and wash-up, but there was a relation between positive cases of IgM and keeping cat at home (P?0.025). More over 51.7% of pregnant women in Gorgan were seronegative and the were prone to acute Toxoplasmosis during their pregnancy. The results of this study confirm that the determination of the diagnostic Toxoplasmosis is an necessary test during pregnancy.

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All the different kind of Mycobacterium species except Tuberculosis Leprae and Bovis are considered as environmental Mycobacterium, which usually can be isolated from sand, water and dusts. In addition to the role of their pathogenesis, they can stimulate the immune systems. The immune mechanisms following Mycobacterium environmental contamination can be either protect or destroy the immune system when facing the disease related to the Mycobacterium. The Golestan province has got 2 regions from the point of view of TB epidemy, it is higher in the east. The aim of this project is determine the prevalency of various types and strains of environmental Mycobacterium in the east and west of the region. The samples were collected from both regions from the wet sand, and muddes covered with grass and following their preparation they were cultured on the Lowen Stein Jenson. The total of samples were 220 from these 120 samples were from the region with epidemy of TB out of these we had 25 samples with positive culture (20.8%) and 47 type of mycobacterium were isolated, the most common forms of them were Mycobacterium Fortuitum (34%), Mycobacterium Flavesens (21.2%), Mycobacterium Chelonae (12.8%). From 100 samples taken from the region with low epidemy of TB 66 samples were with positive culture (66%) which 114 strains of Mycobacterium were diagnosed. The most common forms of these microorganisms were Mycobacterium Flavesens (20.1%), Mycobacterium Chelonae (18.4%) and Mycobacterium (16.6%). In conclusion in all the Golestan province without taking notice the TB epidemy form 220 samples 91% were positive culture (41.2%) which 161 different strains were diagnosed out of these the most common were Mycobacterium Fortuitum (21.8%), Mycobacterium Flavesens (20.5%) and Mycobacterium Chelonae (16.8%). The epidemy and variousity of environmental Mycobacterium in the region with low epidemy were much higher than the region with high epidemy and in regard to that these differences are due to the bioenvironmental factors. So therefore this can indicate that the possibility of higher contact between the immune systems and the environmental Mycobacterium. These Mycobacteriums can act as Booster of BCG vaccine which can continuously stimulate the immune systems and this can help the body’s proper responses when facing with the TB Mycobacterium.