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:: Volume 25, Issue 2 (7-2023) ::
J Gorgan Univ Med Sci 2023, 25(2): 22-31 Back to browse issues page
Effect of a High and Medium Intensity Interval Training Period with Liposomal Alpha-Lipoic Acid Supplementation on the Expression of Sema3A Protein in the Soleus Muscle of the Induced Diabetic Rats
Seyedeh Fatemeh Fatemi1 , Seyed Abdollah Hashemvarzi * 2, Minoo Dadban Shahamat3 , Amin Farzaneh Hessari4
1- Ph.D Candidate in Exercise Physiology, Department of Exercise Physiology, Faculty of Humanities, Sari Branch, Islamic Azad University, Sari, Iran.
2- Assistant Professor, Department of Exercise Physiology, Faculty of Humanities, Sari Branch, Islamic Azad University, Sari, Iran. , hashemvarzi_tkd@yahoo.com
3- Assistant Professor of Sport Physiology, Faculty of Human Sciences, Azadshahr Branch, Islamic Azad University, Azadshahr, Iran.
4- Assistant Professor, Department of Exercise Physiology, Faculty of Humanities, Sari Branch, Islamic Azad University, Sari, Iran.
Abstract:   (506 Views)

Background and Objective: Diabetes leads to impaired blood supply to the peripheral nerves. Sema3A (Semaphorin 3A) is a denervated protein that increases in response to hyperglycemia caused by diabetes. Exercise and alpha-lipoic acid (ALA) supplements can protect against diabetes-induced denervation. This study was done to evaluate the effect of high and moderate-intensity interval training with alpha-lipoic acid supplementation on the expression of Sema3A protein in the soleus muscle of Induced diabetic rats.
Methods: In this experimental study, thirty-five male Wistar rats (weight range: 190-220 g, 6-8 weeks old) were randomly allocated into seven groups of five: healthy control, diabetic, diabetic supplement (S), diabetic high-intensity training (HIT), diabetic moderate-intensity training (MIT), diabetic high intensity+supplement (HIT+S), and diabetic moderate intensity training+supplement (MIT+S). Diabetes was induced by injection of streptozotocin (50 mg/kg/bw). The HIT and MIT protocols were performed five days a week for six weeks. ALA was administered orally at 20 mg/kg daily by gavage. Immunohistochemistry was used to evaluate the expression of Sema3A protein in the soleus muscle. Serum insulin was measured by the ELISA method.
Results: Diabetes leads to increased level of glucose, Sema3A, and a significant decrease in insulin in the soleus muscle compared to healthy (P<0.05). HIT and MIT in combination with ALA, significantly showed lower expression of Sema3A Protein than in the diabetic group (P<0.05).
Conclusion: Although HIT and MIT can reduce the expression of Sema3A protein in the soleus muscle of diabetic rats, combining alpha-lipoic acid supplementation with exercise training is more effective in reducing the amount of denervation.

Keywords: Alpha-lipoic acid [MeSH], Interval Training [MeSH], Semaphorin [MeSH], Diabetes Mellitus [MeSH]
Article ID: Vol25-17
Full-Text [PDF 1038 kb]   (556 Downloads)    
Type of Study: Original Articles | Subject: Exercise Physiology
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Fatemi S F, Hashemvarzi S A, Dadban Shahamat M, Farzaneh Hessari A. Effect of a High and Medium Intensity Interval Training Period with Liposomal Alpha-Lipoic Acid Supplementation on the Expression of Sema3A Protein in the Soleus Muscle of the Induced Diabetic Rats. J Gorgan Univ Med Sci 2023; 25 (2) :22-31
URL: http://goums.ac.ir/journal/article-1-4185-en.html

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Volume 25, Issue 2 (7-2023) Back to browse issues page
مجله دانشگاه علوم پزشکی گرگان Journal of Gorgan University of Medical Sciences
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