|
Investigation of Antioxidant and Antimicrobial Effects of Methanolic Extracts of Berberis integerrima and Graminifolius tragopogon
|
Fahimeh Khodabandeh Shahraki1  , Mojtaba Ranjbar * 2, Mostafa Govahi3 , Majid Tafrihi4 |
1- M.Sc Student of Microbial Biotechnology, Faculty of Biotechnology, Amol University of Special Modern Technologies, Amol, Iran.
2- Associate Professor, Department of Microbial Biotechnology, Faculty of Biotechnology, Amol University of Special Modern Technologies, Amol, Iran. , ranjbarf@ausmt.ac.ir
3- Assistant Professor, Department of Nano Biotechnology, Faculty of Biotechnology, Amol University of Special Modern Technologies, Amol, Iran.
4- Assistant Professor, Department of Molecular Cell Biology, Faculty of Basic Sciences, Mazandaran University, Babolsar, Iran. |
|
|
Abstract: (880 Views) |
Background and Objective: Medicinal plants contain a high level of antioxidant compounds such as flavonoids, phenolic, carotenoids, and tannins, which can be used to eliminate excess free radicals in the body. This study aimed to determine the total phenolic and flavonoid content and to investigate the antioxidant and antibacterial effects of Berberis integerrima and Graminifolius tragopogon methanolic extracts on some Gram-positive and Gram-negative microorganisms.
Methods: In this descriptive-analytical study, methanolic extracts of B. integerrima and G. tragopogon were prepared using 80% methanol. Total phenol and flavonoid contents were determined by Folin–Ciocalteu and aluminum chloride colorimetric methods, respectively. The antioxidant capacity was assessed by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity and reducing power methods. The antibacterial activity of the extracts of B. integerrima and G. tragopogon on Escherichia coli, Bacillus subtilis, Listeria monocytogenes, Staphylococcus aureus, and Salmonella typhimurium were determined by the disk diffusion method. Butylated hydroxytoluene and ciprofloxacin were used as positive controls for antioxidant activity and bacterial strains, respectively.
Results: Total phenol and flavonoid compounds in the extracts of B. integerrima and G. tragopogon were 46.90±0.70 and 22.63±0.59 mg gallic acid per gram of extract and 5.61±0.01 and 46.74±0.81 mg quercetin per gram of extract, respectively. The extracts of B. integerrima and G. tragopogon showed significant antibacterial activity. B. subtilis and S. typhimurium showed the highest sensitivity and resistance to the extracts, respectively. Moreover, the extract of B. integerrima had the most potent inhibitory effect on the examined microorganisms.
Conclusion: B. integerrima extract exhibits higher phenolic content, antioxidant properties, and antimicrobial activity than G. tragopogon extract.
|
|
Keywords: Antioxidant [MeSH], Antimicrobial [MeSH], Berberis Integerrima , Graminifolius Tragopogon
Article ID: Vol24-60 |
|
|
Full-Text [PDF 828 kb]
(2524 Downloads)
|
|
Type of Study: Original Articles |
Subject:
Biochemistry
|
|
|
|
|
|
|
| References |
1. Idris M, Abbas RZ, Masood S, Rehman T, Farooq U, Babar W, et al. The potential of antioxidant rich essential oils against avian coccidiosis. Worlds Poult Sci J. 2016; 73(1): 89-104. doi: 10.1017/S0043933916000787 [ Link] [ DOI] 2. Lai F, Wen Q, Li L, Wu H, Li X. Antioxidant activities of water-soluble polysaccharide extracted from mung bean (Vigna radiata L.) hull with ultrasonic assisted treatment. Carbohydrate Polymers. 2010; 81(2): 323-29. doi: 10.1016/j.carbpol.2010.02.011 [ Link] [ DOI] 3. Lobo V, Patil A, Phatak A, Chandra N. Free radicals, antioxidants and functional foods: Impact on human health. Pharmacogn Rev. 2010 Jul; 4(8): 118-26. doi: 10.4103/0973-7847.70902 [ DOI] [ PubMed] 4. Andleeb S, Alsalme A, Al-Zaqri N, Warad I, Alkahtani J, Bukhari SM. In-vitro antibacterial and antifungal properties of the organic solvent extract of Argemone mexicana L. Journal of King Saud University – Science. 2020 Apr; 32(3): 2053-58. doi: 10.1016/j.jksus.2020.01.044 [ DOI] [ PubMed] 5. Ceylan S, Cetin S, Camadan Y, Saral O, Ozsen O, Tutus A. Antibacterial and antioxidant activities of traditional medicinal plants from the Erzurum region of Turkey. Ir J Med Sci. 2019 Nov; 188(4): 1303-309. doi: 10.1007/s11845-019-01993-x [ DOI] [ PubMed] 6. Alreshidi M, Noumi E, Bouslama L, Ceylan O, Veettil VN, Adnan M, et al. Phytochemical Screening, Antibacterial, Antifungal, Antiviral, Cytotoxic, and Anti-Quorum-Sensing Properties of Teucrium polium L. Aerial Parts Methanolic Extract. Plants (Basel). 2020 Oct; 9(11): 1418. doi: 10.3390/plants9111418 [ DOI] [ PubMed] 7. Hadadi Z, Nematzadeh GA, Ghahari S. A study on the antioxidant and antimicrobial activities in the chloroformic and methanolic extracts of 6 important medicinal plants collected from North of Iran. BMC Chem. 2020 Apr; 14(1): 33. doi: 10.1186/s13065-020-00683-5 [ DOI] [ PubMed] 8. Gonelimali FD, Lin J, Miao W, Xuan J, Charles F, Chen M, et al. Antimicrobial Properties and Mechanism of Action of Some Plant Extracts Against Food Pathogens and Spoilage Microorganisms. Front Microbiol. 2018 Jul; 9: 1639. doi: 10.3389/fmicb.2018.01639 [ DOI] [ PubMed] 9. Ahmadpour Torki M, Ranjbar M, Govahi M, Tafrihi M. [Effect of Aqueous Extract of Turkey Tail (Trametes versicolor) on Staphylococcus aureus, Escherichia coli and Fusarium thapsinum]. J Gorgan Univ Med Sci. 2022; 24(3): 93-98. [Article in Persian] [ View at Publisher] 10. Behravan M, Hossein Panahi A, Naghizadeh A, Ziaee M, Mahdavi R, Mirzapour A. Facile green synthesis of silver nanoparticles using Berberis vulgaris leaf and root aqueous extract and its antibacterial activity. Int J Biol Macromol. 2019 Mar; 124: 148-54. doi: 10.1016/j.ijbiomac.2018.11.101 [ DOI] [ PubMed] 11. Anzabi Y. Biosynthesis of ZnO nanoparticles using barberry (Berberis vulgaris) extract and assessment of their physico-chemical properties and antibacterial activities. Green Processing and Synthesis. 2018; 7(2): 114-21. doi: 10.1515/gps-2017-0014 [ Link] [ DOI] 12. Imenshahidi M, Hosseinzadeh H. Berberine and barberry (Berberis vulgaris): A clinical review. Phytother Res. 2019 Mar; 33(3): 504-23. doi: 10.1002/ptr.6252 [ DOI] [ PubMed] 13. Amirghofran Z, Azadbakht M, Karimi MH. Evaluation of the immunomodulatory effects of five herbal plants. J Ethnopharmacol. 2000 Sep; 72(1-2): 167-72. doi: 10.1016/s0378-8741(00)00234-8 [ DOI] [ PubMed] 14. Hayouni EA, Abedrabba M, Bouix M, Hamdi M. The effects of solvents and extraction method on the phenolic contents and biological activities in vitro of Tunisian Quercus coccifera L. and Juniperus phoenicea L. fruit extracts. Food Chem. 2007; 105(3): 1126-34. Doi: 10.1016/j.foodchem.2007.02.010 [ Link] [ DOI] 15. Prevc T, Segatin N, Ulrih NP, Cigić B. DPPH assay of vegetable oils and model antioxidants in protic and aprotic solvents. Talanta. 2013 May; 109: 13-19. doi: 10.1016/j.talanta.2013.03.046 [ DOI] [ PubMed] 16. Ranjbar M, Kiani M, Nikpey A. Antioxidant and scolicidal activities of four Iranian Mentha species (Lamiaceae) in relation to phenolic elements. J Herbmed Pharmacol. 2020; 9(3): 200-208. doi: 10.34172/jhp.2020.26 [ View at Publisher] [ DOI] 17. Khan ZUH, Sadiq HM, Shah NS, Khan AU, Muhammad N, Hassan SU, et al. Greener synthesis of zinc oxide nanoparticles using Trianthema portulacastrum extract and evaluation of its photocatalytic and biological applications. J Photochem Photobiol B. 2019 Mar; 192: 147-57. doi: 10.1016/j.jphotobiol.2019.01.013 [ DOI] [ PubMed] 18. Tanruean K, Poolprasert P, Suwannarach N, Kumla J, Lumyong S. Phytochemical Analysis and Evaluation of Antioxidant and Biological Activities of Extracts from Three Clauseneae Plants in Northern Thailand. Plants (Basel). 2021 Jan; 10(1): 117. doi: 10.3390/plants10010117 [ DOI] [ PubMed] 19. Farag RS, Abdel-Latif MS, Abd El Baky HH, Tawfeek LS. Phytochemical screening and antioxidant activity of some medicinal plants' crude juices. Biotechnol Rep (Amst). 2020 Oct; 28: e00536. doi: 10.1016/j.btre.2020.e00536 [ DOI] [ PubMed] 20. Zovko Koncić M, Kremer D, Karlović K, Kosalec I. Evaluation of antioxidant activities and phenolic content of Berberis vulgaris L. and Berberis croatica Horvat. Food Chem Toxicol. 2010 Aug-Sep; 48(8-9): 2176-80. doi: 10.1016/j.fct.2010.05.025 [ DOI] [ PubMed] 21. Motalleb G, Hanachi P, Kua SH, Fauziah O, Asmah R. Evaluation of Phenolic Content and Total Antioxidant Activity in Berberis vulgaris Fruit Extract. Journal of Biological Sciences. 2005; 5: 648-53. doi: 10.3923/jbs.2005.648.653 [ Link] [ DOI] 22. Dorman HJ, Koşar M, Kahlos K, Holm Y, Hiltunen R. Antioxidant properties and composition of aqueous extracts from Mentha species, hybrids, varieties, and cultivars. J Agric Food Chem. 2003 Jul; 51(16): 4563-69. doi: 10.1021/jf034108k [ DOI] [ PubMed] 23. Arumugam P, Ramamurthy P, Ramesh A. Antioxidant and Cytotoxic Activities of Lipophilic and Hydrophilic Fractions of Mentha Spicata L. (Lamiaceae). Int J Food Prop. 2010; 13(1): 23-31. doi: 10.1080/10942910802144329 [ Link] [ DOI] 24. Mekonnen A, Desta W. Comparative study of the antioxidant and antibacterial activities of Rumex abyssinicus with commercially available Zingiber officinale and Curcuma longa in Bahir Dar city, Ethiopia. Chem Biol Technol Agric. 2021; 8: 2. doi: 10.1186/s40538-020-00198-0 [ Link] [ DOI] 25. Elansary HO, Szopa A, Klimek-Szczykutowicz M, Ekiert H, Barakat AA, Al-Mana FA. Antiproliferative, antimicrobial, and antifungal activities of polyphenol extracts from Ferocactus species. Processes. 2020; 8(2): 138. doi: 10.3390/pr8020138 [ Link] [ DOI] 26. Koné WM, Atindehou KK, Kacou-N'douba A, Dosso M. Evaluation of 17 medicinal plants from Northern Côte d'Ivoire for their in vitro activity against Streptococcus pneumoniae. Afr J Tradit Complement Altern Med. 2006 Aug; 4(1): 17-22. [ PubMed] |
|
| Send email to the article author |
|
|
|
Khodabandeh Shahraki F, Ranjbar M, Govahi M, Tafrihi M. Investigation of Antioxidant and Antimicrobial Effects of Methanolic Extracts of Berberis integerrima and Graminifolius tragopogon. J Gorgan Univ Med Sci 2023; 24 (4) :118-125 URL: http://goums.ac.ir/journal/article-1-4078-en.html
|
