:: Volume 23, Issue 4 (Winter 2022) ::
J Gorgan Univ Med Sci 2022, 23(4): 81-88 Back to browse issues page
Antibacterial and Hemolytic Effect of Nanoparticles Zinc / Ferrite / Cellulose Bioconjugated with Vancomycin Antibiotics
Minoo Akbari1 , Ali Hossein Rezayan * 2, Hossein Rastegar3, Mahmoud Alebouyeh4
1- Ph.D Candidate in Nanobiotechnology, Department of Life Science Engineering, Faculty of New Sciences and Technologies, University of Tehran, Tehran, Iran..
2- Associate Professor, Department of Life Science Engineering, Faculty of New Sciences and Technologies, University of Tehran, Tehran, Iran. , ahrezayan@ut.ac.ir
3- Professor, Cosmetic Products Research Center, Iran Food and Drug Administration, Ministry of Health and Medical Education, Tehran, Iran.
4- Associate Professor, Iran Food and Drug Administration, Ministry of Health and Medical Education, Tehran, Iran.
Abstract:   (3028 Views)
Background and Objective: Binding of antibiotics to nanoparticles increases the antibacterial potential of nanoparticles and antibiotics. This study was performed to determine the antibacterial and hemolytic effect of zinc / ferrite / cellulose nanocomposite (ZnFe2O4 @ Cell) (single nanoparticle), zinc / ferrite / cellulose nanocomposite was aminated with 3-aminopropyltriethoxysilane (APTES) with the name of ZnFe2O4@Cell@APTES (Coated nanocomposite) and ZnFe2O4@Cell@APTES@Van nanocomposite (coated nanocomposite bound to vancomycin) against gram-negative bacteria Escherichia coli (E. coli) and Pseudomonas aeruginosa (P. aeruginosa) and gram-positive bacterium Staphylococcus aureus (S. aureus).
Methods: In this descriptive study, antibacterial-activity was evaluated by broth macro dilution method. Minimum inhibitory concentration (MIC) and minimum lethal concentration (MBC) were determined for E. coli, S. aurous and P. aeruginosa. The hemolytic activity of nanoparticles was investigated by colorimetric method.
Results: Nanoparticles did not have hemolytic activity. ZnFe2O4@Cell and ZnFe2O4@Cell@APTES@Van did not have a significant antibacterial effect against gram-positive and gram-negative bacteria, and vancomycin binding resulted in antibacterial-activity. ZnFe2O4@Cell@APTES@Van inhibited the growth of Gram-negative bacteria Escherichia coli and Pseudomonas aeruginosa. The growth of E. coli was reduced to 85% at a concentration of 0.4 mg/ml and a concentration of 0.1 mg nanoparticles completely prevented the growth of P. aeruginosa. The growth of gram-positive S. aureus bacteria at a concentration of 0.3 mg/ml nanoparticles was completely stopped.
Conclusion: Vancomycin-modified nanocomposite has antibacterial-activity against both gram-positive and gram-negative bacteria and has the potential to overcome the antibiotic resistance of bacteria.
Keywords: Nanoparticles [MeSH], Escherichia coli [MeSH], Staphylococcus aureus [MeSH], Pseudomonas aeruginosa [MeSH], ZnFe2O4@Cell@APTES@Van ,
Article ID: Vol23-58
Full-Text [PDF 851 kb]   (1437 Downloads)    
Type of Study: Original Articles | Subject: Nanobiotecnology
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