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:: Volume 22, Issue 2 (Summer 2020) ::
J Gorgan Univ Med Sci 2020, 22(2): 40-48 Back to browse issues page
Effect of resistance training and Resveratrol supplementation on muscle regeneration of MyoD and eMHC in CT-26 colon cancer mice
Enayatollah Asadmanesh1 , Maryam Koushkie Jahromi * 2, Mahdi Samadi3 , Farhad Daryanoosh4 , Javad Neamati5
1- Assistant Professor in Exercise Biochemistry and Metabolism, Department of Sport Sciences, School of Education and Psychology, Shiraz University, Shiraz, Iran
2- Associate Professor of Exercise Physiology, Department of Sport Sciences, School of Education and Psychology, Shiraz University, Shiraz, Iran , koushkie53@yahoo.com
3- Ph.D in Exercise Biochemistry and Metabolism, Department of Sport Sciences, School of Education and Psychology, Shiraz University, Shiraz, Iran
4- Associate Professor of Exercise Physiology, Department of Sport Sciences, School of Education and Psychology, Shiraz University, Shiraz, Iran
5- Assistant Professor of Exercise Physiology, Department of Sport Sciences, School of Education and Psychology, Shiraz University, Shiraz, Iran
Abstract:   (547 Views)
Background and Objective: Physical exercise and nutrition supplements are recommended interventions to reduce cachexia. This study was conducted to determine the effect of resistance training and resveratrol supplementation on muscle regeneration indices of MyoD (myoblast determination protein) and eMHC (embryonic Myosin Heavy Chain) in CT-26 colon cancer mice.
Methods: This experimental study was performed on 20 six-week-old BALB/c mice to which CT-26 tumor was implanted. The mice were divided into four groups of resistance training, resveratrol, combination of resveratrol with resistance training, and control. The resistance training group performed six weeks of progressive resistance training. The resveratrol group received 100 mg/kg resveratrol per day, and the control and resistance training group received the solution of Methyl cellulose through gavage. In gastrocnemius muscle MyoD protein and eMHC level were measured using western blot and ELISA methods, respectively.
Results: eMHC protein in combination of resveratrol with exercise group (4.66±0.25) increased significantly compared to the exercise group (3.46±0.64) (P<0.05). Body weight of mice without tumor (21.50±1.30) in the resistance training group increased significantly compared to other groups (P<0.05). There was no significant difference in the tumor weight of mice and MyoD protein in expermental groups.
Conclusion: Regarding the increasing effect of combination of resveratrol with exercise group compared to resistance training group on eMHC, resveratrol supplementation at higher doses may be recommended along with resistance training to improve muscle regeneration.
Keywords: Resistance Training [MeSH], Resveratrol [MeSH], MyoD Protein [MeSH], Cancer [MeSH],
Article ID: Vol22-21
Full-Text [PDF 277 kb]   (62 Downloads)    
Type of Study: Original Articles | Subject: Exercise Physiology
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Asadmanesh E, Koushkie Jahromi M, Samadi M, Daryanoosh F, Neamati J. Effect of resistance training and Resveratrol supplementation on muscle regeneration of MyoD and eMHC in CT-26 colon cancer mice. J Gorgan Univ Med Sci. 2020; 22 (2) :40-48
URL: http://goums.ac.ir/journal/article-1-3637-en.html


Volume 22, Issue 2 (Summer 2020) Back to browse issues page
مجله علمی دانشگاه علوم پزشکی گرگان Journal of Gorgan University of Medical Sciences
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