[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 22, Issue 2 (Summer 2020) ::
J Gorgan Univ Med Sci 2020, 22(2): 34-39 Back to browse issues page
Effect of nanochitin on tissue absorption rate of lead acetate in rat liver
Azam Khazaeipour1 , Somaye Namroodi * 2, Shohre Taziki3
1- Departement of Environmental Sciences, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
2- Assistant Professor of Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran , namroodi@gau.ac.ir
3- Assistant Professor, Department of Pharmacology, Golestan University of Medical Sciences, Gorgan, Iran
Abstract:   (598 Views)
Background and Objective: Lead is one of the most toxic environmental pollutants, which is very dangerous for living creatures, including humans. Therefore, the use of various compounds such as nanochitin to remove lead from aquatic environments has been considered in recent years. This study was performed to determine the effect of nanochitin on the Tissue absorption rate of lead acetate in the liver of rats.
Methods: This experimental study was performed on 15 Wistar rats with a weight of 150-180 grams and an age of 8-10 weeks. The animals were allocated into three groups including control group, the lead group, and the nanochitin and lead simultaneously group. Animals were received lead and nanochitin by gavage for six weeks. Then liver tissue was removed and lead concentration was measured with an atomic absorption device. Liver tissue was prepared for hematoxylin-eosin staining and then examined under an optical microscope.
Results: The mean lead concentration in the liver tissue of the control, lead and the nanochitin and lead simultaneously groups were 8.1±0.45, 45.41±4.73, and 17.06±0.83 mic/g, respectively. The concentration of lead in the lead group increased significantly compared to the control group (P<0.05). Also, the hepatic concentration of lead in the nanochitin and lead simultaneously group significantly reduced in compared to lead group (P<0.05). Histopathological studies showed a reduction in tissue lesions including degeneration and necrosis of liver hepatocytes, hypertension, and severe congestion in liver tissue in the nanochitin and lead simultaneously group in compared to lead group.
Conclusion: Nanochitin without adverse effects on liver tissue can increase the removal and inhibit the absorption of lead in rats liver tissue.
Keywords: Nanochitin , Lead [MeSH], Liver [MeSH], Rat [MeSH],
Article ID: Vol22-20
Full-Text [PDF 338 kb]   (60 Downloads)    
Type of Study: Original Articles | Subject: Pharmacology
1. Nwokocha CR, Younger-Coleman N, Nwokocha M, Owu DU, Iwuala M. Investigation of effects of time of measurement and modes of administration on cadmium accumulation in rat liver under some medicinal plants food supplemented diet. Pharmacognosy Res. 2014 Jul; 6(3): 240-45. DOI: 10.4103/0974-8490.132604
2. Nassiri M, Khaki A, Bazi P, Khaki A, Sahizadeh R, Sahizadeh A. [Ultra-Structure Study of Lead Acetate Cytotoxic Effects on Testis in Rabbit]. Armaghane Danesh. 2008; 13(1): 45-53. [Article in Persian]
3. Lee SH, Lee JS, Choi YJ, Kim JG. In situ stabilization of cadmium-, lead-, and zinc-contaminated soil using various amendments. Chemosphere. 2009 Nov; 77(8): 1069-75. DOI: 10.1016/j.chemosphere.2009.08.056
4. Flora SJ, Pachauri V. Chelation in metal intoxication. Int J Environ Res Public Health. 2010 Jul; 7(7): 2745-88. DOI: 10.3390/ijerph7072745
5. Tan TS, Chin HY, Tsai ML, Liu CL. Structural alterations, pore generation, and deacetylation of α- and β-chitin submitted to steam explosion. Carbohydr Polym. 2015 May; 122: 321-28. DOI: 10.1016/j.carbpol.2015.01.016
6. Toiserkani H, Sadaghat F. [Chitin and Chitosan: Structure, Properties and Applications]. J Aqu Eco. 2013; 2(3): 40-26. [Article in Persian]
7. Jaafarzadeh N, Mengelizadeh N, Takdastan A, Heidari-Farsani M, Niknam N. Adsorption of Zn (II) from aqueous solution by using chitin extraction from crustaceous shell. J Adv Environ Health Res. 2014; 2(2): 110-19. DOI: 10.22102/JAEHR.2014.40151
8. Becker T, Schlaak M, Strasdeit H. Adsorption of nickel (II), zinc (II) and cadmium (II) by new chitosan derivatives. Reactive and Functional Polymers. 2000; 44(3): 289-98. https://doi.org/10.1016/S1381-5148(99)00104-2
9. Liu D, Li Z, Zhu Y, Li Z, Kumar R. Recycled chitosan nanofibril as an effective Cu(II), Pb(II) and Cd(II) ionic chelating agent: adsorption and desorption performance. Carbohydr Polym. 2014 Oct; 111: 469-76. DOI: 10.1016/j.carbpol.2014.04.018
10. Shokraei S, Rouhani A, Nazari M, Manafi S. [Chromium Elimination from Water by use of Iron Oxide Nanoparticles Absorbents]. Journal of Toloo-e-Behdasht. 2014; 13(3): 160-70. [Article in Persian]
11. Al-Attar AM. Antioxidant effect of vitamin E treatment on some heavy metals-induced renal and testicular injuries in male mice. Saudi J Biol Sci. 2011 Jan; 18(1): 63-72. DOI: 10.1016/j.sjbs.2010.10.004
12. Nadapdap TP, Lutan D, Arsyad KHM, Ilyas S. Influence of Chitosan from Shrimp Skin to Quality and Quantity of Sperm of Albino Rats after Administration of Lead. Andrology. 2014; 3: 114. DOI: 10.4172/2167-0250.1000114
13. Zarei K, Najafpour Gh, Sharifzadeh M. [Synthesis and Application of Nano-chitosan in Removal of Heavy Metals]. Thesis in Chemical. Faculty of Chemical Engineering. Babol Noshirvani University of Technology (BUT). 2012. [Persian]
14. Mokhtari M, Shariati M, Gashmardi N. [Effect of lead on thyroid hormones and liver enzymes in adult male rats]. Hormozgan Med J. 2007; 11(2): 115-20. [Article in Persian]
15. Alehi M, Fatahian S, Shahanipour K. [Effect of iron oxide Nanoparticles coated with chitosan on renal functional indeces in rats]. J Gorgan Univ Med Sci. 2017; 19(1): 14-19. [Article in Persian]
16. Marianti A, Anatiasara D, Ashar FF. Chitosan as chelating and protective agents from lead intoxication in rat. Biosaintifika: Journal of Biology & Biology Education. 2017; 9(1): 126-33. DOI: 10.15294/biosaintifika.v9i1.8943
17. Muzzarelli R, Muzzarelli C, Cosani A, Terbojevich M. 6-Oxychitins, novel hyaluronan-like regiospecifically carboxylated chitins. Carbohydrate Polymers. 1999; 39(4): 361-67. https://doi.org/10.1016/S0144-8617(99)00027-2
18. Anderson AC, Pueschel SM, Linakis JG. Pathophysiology of Lead Poisoning. In: Pueschel SM, Linakis JG, Anderson AC. Lead Poisoning in Childhood. Baltimore: Brookes. 1996; pp: 75-96.
19. Bellinger D, Dietrich KN. Low-level lead exposure and cognitive function in children. Pediatric Annals. 1994; 23(11): 600-605. https://doi.org/10.3928/0090-4481-19941101-08
20. Popova M, Popov CS. Effect of heavy metal salts on the activity of rat liver and kidney catalase and lysosomal hydrolases. J Vet Med A. 1998; 45(1-10): 343-51. https://doi.org/10.1111/j.1439-0442.1998.tb00837.x
21. Massó EL, Corredor L, Antonio MT. Oxidative damage in liver after perinatal intoxication with lead and/or cadmium. J Trace Elem Med Biol. 2007; 21(3): 210-16. DOI: 10.1016/j.jtemb.2007.03.002
22. Nehru B, Kaushal S. Alterations in the hepatic enzymes following experimental lead poisoning. Biol Trace Elem Res. 1993 Jul; 38(1): 27-34. DOI: 10.1007/BF02783979
23. Struzyńska L, Dabrowska-Bouta B, Rafałowska U. Acute lead toxicity and energy metabolism in rat brain synaptosomes. Acta Neurobiol Exp (Wars). 1997; 57(4): 275-81.
24. Zahedi A, Khaki A, Bazi P, Khaki A. [The Hepato Toxic Effects of Lead Acetate on Hepatic Tissues in New Zealand Ian Rabbit]. J Guilan Univ Med Sci. 2009; 18(1): 17-24. [Article in Persian]
25. Wang Z, Yan Y, Yu X, Li W, Li B, Qin C. Protective effects of chitosan and its water-soluble derivatives against lead-induced oxidative stress in mice. Int J Biol Macromol. 2016 Feb; 83: 442-49. DOI: 10.1016/j.ijbiomac.2015.10.017
26. Levitskaia TG, Creim JA, Curry TL, Luders T, Morris JE, Sinkov SI, et al. Investigation of chitosan for decorporation of 60Co in the rat. Health Phys. 2009 Aug; 97(2): 115-24. DOI: 10.1097/01.HP.0000346798.82764.d7
27. Abdel-Gawad EI, Awwad SA. In-vivo and in-vitro prediction of the efficiency of nano-synthesized material in removal of lead nitrate toxicity. J Am Sci. 2011; 7(1): 105-19.
Send email to the article author

XML   Persian Abstract   Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Khazaeipour A, Namroodi S, Taziki S. Effect of nanochitin on tissue absorption rate of lead acetate in rat liver. J Gorgan Univ Med Sci. 2020; 22 (2) :34-39
URL: http://goums.ac.ir/journal/article-1-3584-en.html

Volume 22, Issue 2 (Summer 2020) Back to browse issues page
مجله علمی دانشگاه علوم پزشکی گرگان Journal of Gorgan University of Medical Sciences
Persian site map - English site map - Created in 0.06 seconds with 29 queries by YEKTAWEB 4256