[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Home::
Journal Information::
Indexing Sources::
Editorial Board::
Executive Members::
Articles Archive::
Instruction to Authors::
Peer-Review::
Contact Us::
Site Facilities::
::
Search in website

Advanced Search
Receive site information
Enter your Email in the following box to receive the site news and information.

Happy Persian New Year (Nowruz)


:: Volume 21, Issue 4 (12-2019) ::
J Gorgan Univ Med Sci 2019, 21(4): 79-85 Back to browse issues page
Frequency of mecA and blaZ genes in isolates of Staphylococcus aureus isolated from clinical samples by PCR method
Yasaman Rahnama1 , Ailar Jamalli 2, Teena Dadgar3
1- M.Sc in Microbiology, Department of Biology, Gorgan Branch, Islamic Azad University, Gorgan, Iran
2- Associate Professor, Laboratory Sciences Research Center, Golestan University of Medical Sciences, Gorgan, Iran , jamali@goums.ac.ir
3- Assistant Professor, Department of Biology, Gorgan Branch, Islamic Azad University, Gorgan, Iran
Abstract:   (6692 Views)
Background and Objective: Staphylococcus aureus (S. aureus) is the most common cause of nosocomial infections. Treatment of Staphylococcal infections has become more complicated due to the emergence of methicillin-resistant S.aureus (MRSA) strains. This study was done to determine the frequency of methicillin resistance encoding gene (mecA) and β-lactamase resistance encoding gene (blaZ) in S. aureus isolates from clinical samples using Polymerase Chain Reaction (PCR) method.
Methods: This descriptive-analytic study was carried out on 59 S. aureus isolates from clinical samples in Gorgan hospitals from January-February 2017 to June-July 2017. All the isolates were identified using gram staining, catalase test, tube coagulase test, growth on Mannitol salt agar medium and the DNase test in the Microbiology Laboratory .Antibiotic resistance was evaluated using the standard disk diffusion.  Iodometric method was used to detect β-lactamase production / activity in this bacterium. PCR test was done to detect mecA and blaZ genes.
Results: All S. aureus isolates (100%) clinical samples possessed blaZ gene, followed by 27 isolates (45.8%) possessed mecA gene (MRSA), which these isolates possessed mecA gene were concurrently positive for blaZ gene. 5% of oxacillin-resistant strains and 3% of cefoxitin-resistant strains possessed mecA gene and 47 isolates (79.4%) carrying blaZ gene were β-lactamase-positive in phenotypic method.
Conclusion: This study showed that in all clinical samples isolated S. aureus isolates which these isolates possessed mecA gene were concurrently positive for blaZ gene.
Keywords: Staphylococcus aureus, blaZ gene, mecA gene, PCR
Full-Text [PDF 268 kb]   (12656 Downloads)    
Type of Study: Original Articles | Subject: Microbiology
References
1. Ryu S, Song PI, Seo CH, Cheony H, Park Y. Colonization and Infection of the Skin by S. aureus: Immune System Evasion and the Response to Cationic Antimicrobial Peptides. Int J Mol Sci. 2014 May; 15(5): 8753-72. doi: 10.3390/ijms15058753
2. Mah FS, Davidson R, Holland EJ, Hovanesian J, John T, Kanellopoulos J, et al. Current knowledge about and recommendations for ocular methicillin-resistant Staphylococcus aureus. J Cataract Refract Surg. 2014 Nov; 40(11): 1894-908. doi: 10.1016/j.jcrs.2014.09.023
3. Alaouadi RF. Comparative phenotypic and genotypic study of MSSA and MRSA wound infections in Babylon. AL-Qadisiya Journal of Vet Med Sci. 2015; 14(2): 23-33. doi: https://doi.org/10.29079/vol14iss2art346
4. Nwokah EG, Abbey SD, Wachukwu CK. mecA Gene Profile of Methicillin-Resistant StaphylococcusaureusIsolates from Clinical Sources in Port Harcourt, Nigeria. American Journal of Biomedical and Life Sciences. 2016; 4(3): 41-48. doi: 10.11648/j.ajbls.20160403.14
5. Rostamzad A, Rostamneia N, Pourahmad F, Shamsi M. Determination of vancomycin and methicillin resistance in clinical isolates of Staphylococcus aureus in hospitals of Ilam city. Journal of Basic Research in Medical Scineces. 2016; 3(3): 1-6. doi: 10.18869/acadpub.jbrms.3.3.1
6. Reddy PN, Srirama K, Dirisala VR. An Update on Clinical Burden, Diagnostic Tools, and Therapeutic Options of Staphylococcus aureus. Infect Dis (Auckl). 2017 May; doi: 10.1177/1179916117703999. eCollection 2017.
7. Chambers HF, Deleo FR. Waves of Resistance: Staphylococcus aureus in the Antibiotic Era. Nat Rev Microbiol. 2009 Sep; 7(9): 629-41. doi: 10.1038/nrmicro2200
8. Solberg CO. Spread of Staphylococcus aureus in hospitals: causes and prevention. Scand J Infect Dis. 2000; 32(6): 587-95.
9. Amr GE, Al Gammal S. Emergence of Vancomycin Resistant Staphylococcus aureusIsolated from Patients in ICUs of Zagazig University Hospitals. The Egyptian Journal of Medical Microbiology. 2017 Apr; 26(2): 53-59. doi: 10.12816/0046229
10. Chaudhary M, Payasi A. Surveillance Study for MRSA Prevalence and Susceptibility Trends Against mecAand vanAPositive Clinical Isolates. International Journal of Advances in Pharmacy, Biology and Chemistry. 2015; 4(2): 469-77.
11. Olsen JE, Christensen H, Aarestrup FM. Diversity and evolution of blaZ from Staphylococcus aureus and coagulase-negative staphylococci. J Antimicrob Chemother. 2006 Mar; 57(3): 450-60. doi: 10.1093/jac/dki492
12. Raei F, Eftekhar F. [Studying the presence of blaZ gene and β-lactamase production in clinical isolates of Staphylococcus epidermidis]. Iran J Med Microbiol. 2008; 2(2): 35-41. [Article in Persian]
13. Jeshina J, Surekha K. Molecclar Characterization of Methicillin Resistant S.aureusStrains Isolated in Kerala, South India. Current Research in Bacteriology. 2009; 2(1): 1-6. doi: 10.3923/crb.2009.1.6
14. Jomehpour N, Rezaei Manesh MR. [Investigation of the frequency of Staphylococcus aureus carriers and its methicillin-resistant pattern in TorbatHeydariyeh hospitals staff in 2013]. Journal of Tanin Salamat (Health Chimes). 2016; 4(1): 42-49. [Article in Persian]
15. Bokaeian M, Tahmasebi H, Shahraki Zahedani Sh, Adabi J. [An investigation of toxic shock syndrome Toxin-1Gene in methicilin-resistant clinical strains of Staphylococcus aureus using multiplex PCR method]. Qom Univ Med Sci J. 2017; 11(1): 57-67. [Article in Persian]
16. Izanloo A, Bahreini M, Sharifmoghadam MR, Safamanesh S, Azimian A. [Evaluation of vancomycin resistance by Phenotypic and genotypic methods among S. aureus strains isolated from patients]. J North Khorasan Univ Med Sci. 2016; 8(2): 215-24. [Article in Persian]
17. Sobhani Poor MH, Mansouri S, Saeidadeli N. [Prevalence of Methicillin-Resistant Staphylococcus aureus (MRSA) and Antibiotic Resistance Patterns of the Isolates from the Nose of Training Soldiers in Kerman in 2012]. Iran J Med Microbiol. 2014; 8(3): 15-21. [Article in Persian]
18. Swenson JM, Spargo J, Tenover FC, Ferraro MJ. Optimal inoculation methods and quality control for the NCCLS oxacillin agar screen test for detection of oxacillin resistance in Staphylococcus aureus. J Clin Microbiol. 2001 Oct;39(10):3781-4. doi: 10.1128/JCM.39.10.3781-3784.2001
19. Clinical and Laboratory Standards Institute / NCCLS Performance standards for Antimicrobial disc diffusion tests; Approved standards. 9th ed. CLSI Document M2-M9. Wayne Pa: Clinical and Laboratory Standards Institute; 2006.
20. Miller SA, Dykes DD, Polsky HF. A simply salting outprocedure for extracting DNA from human nucleated cells. Nucleic Acids Res. 1988 Feb; 16(3): 1215. doi: 10.1093/nar/16.3.1215
21. Hallin M, Friedrich AW, Struelens MJ. spa typing for epidemiological surveillance of Staphylococcus aureus. Methods Mol Biol. 2009; 551:189-202. doi: 10.1007/978-1-60327-999-4_15
22. Shakeri F, Shojai A, Golalipour M , Rahimi Alang S, Vaez H, et al. Spa Diversity among MRSA and MSSA Strains of Staphylococcus aureus in North of Iran. Int J Microbiol. Volume 2010, Article ID 351397. http://dx.doi.org/10.1155/2010/351397
23. Rahimi H, Dastmalchi Saei H, Ahmadi M. Nasal Carriage of Staphylococcus aureus: Frequency and Antibiotic Resistance in Healthy Ruminants. Jundishapur J Microbiol. 2015 Oct; 8(10): e22413. doi: 10.5812/jjm.22413
24. Nowroozi J, Pakzad P, Ebrahimi E, Razavipour R. [Detection of biocide resistance genes, qac A/B and smr, among isolated Staphylococcus aureus from clinical and non-clinical sources]. Pajoohande. 2011; 16 (2) :83-91. [Article in Persian]
25. Pournajaf A, Ardebili A, Goudarzi L, Khodabandeh M, Narimani T, Abbaszadeh H. PCR-based identification of methicillin-resistant Staphylococcus aureusstrains and their antibiotic resistance profiles. Asian Pac J Trop Biomed. 2014 May; 4(Suppl 1): S293-S297. doi: 10.12980/APJTB.4.2014C423
26. Khoei F, Mobaiyen H, Nahaei MR, Sadeghi Mohammadi S. Antibiotic Resistance Pattern and Frequency of mecA Gene in Staphylococcus aureus Isolated from Shohada Hospital, Tabriz. Journal of Medical Microbiology and Infectious Diseases. 2014; 2(3): 105-108.
27. Gomes RMF, Bomfim MRQ, Trindade MJV, Farias LM, Santos SG. Potential Spread of Methicillin-Resistant Staphylococcus aureus Recovered from Patients with Bloodstream Infection. Chemo Open Access. 2015; 4(2): 149. doi: 10.4172/2167-7700.1000149
28. Kim GY, Lee CH. Antimicrobial susceptibility and pathogenic genes of Staphylococcus aureus isolated from the oral cavity of patients with periodontitis. J Periodontal Implant Sci. 2015 Dec; 45(6): 223-28. doi: 10.5051/jpis.2015.45.6.223
29. Naseer BS, Jayaraj YM. Identification of Multi-Resistant Staphylococcus aureus in Clinical Specimens. Res J Med Sci. 2010; 4(3): 204-207. doi: 10.3923/rjmsci.2010.204.207
30. Khattak SU, Bacha N, Lutfullah G, Bakht J, Ali S, Ali J, et al. Study of the genetic traits associated with antibiotic resistance in Staphylococcus aureus isolated from skin wards of Khyber Pakhtunkhwa, Pakistan. Asian Pac J Trop Dis. 2015; 5(5): 393-98. https://doi.org/10.1016/S2222-1808(14)60803-3
31. Saderi H, Owlia P, Shahrbanooie R. Vancomycin resistance among clinical isolates of Staphylococcus aureus. Arch Iranian Med. 2005; 8(2): 100-103.
Send email to the article author


XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Rahnama Y, Jamalli A, Dadgar T. Frequency of mecA and blaZ genes in isolates of Staphylococcus aureus isolated from clinical samples by PCR method. J Gorgan Univ Med Sci 2019; 21 (4) :79-85
URL: http://goums.ac.ir/journal/article-1-3503-en.html


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 21, Issue 4 (12-2019) Back to browse issues page
مجله دانشگاه علوم پزشکی گرگان Journal of Gorgan University of Medical Sciences
Persian site map - English site map - Created in 0.05 seconds with 38 queries by YEKTAWEB 4645