[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Home::
Journal Information::
Indexing Sources::
Editorial Board::
Executive Members::
Articles Archive::
Instruction to Authors::
Peer-Review::
Contact Us::
Site Facilities::
::
Search in website

Advanced Search
Receive site information
Enter your Email in the following box to receive the site news and information.

Happy Persian New Year (Nowruz)


:: Volume 21, Issue 3 (10-2019) ::
J Gorgan Univ Med Sci 2019, 21(3): 49-56 Back to browse issues page
Copper oxide nanoparticles toxicity on pituitary gonadal axis and spermatogenesis in male rats
Sajjad Rajabi1 , Ali Noori 2, Fatemeh Shahbazi3
1- M.Sc in Animal Physiology, Payam Noor University of Tehran, Iran
2- Assistant Professor, Department of Biology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran , ali.noori55@gmail.com
3- Assistant Professor, Department of Biology, Payame Noor University, Iran
Abstract:   (6069 Views)
Background and Objective: Copper oxide nanoparticles with unique properties have numerous biological applications with probably toxicity. This study was conducted to determine the toxicity of copper oxide nanoparticles on the pituitary-gonadal axis and spermatogenesis in male rats.
Methods: In this experimental study, 40 male Wistar rats were randomly allocated into 4 groups including control group and three intervention  groups which receiving the cancentration of 10, 20 and 30 mg/kg of copper oxide nanoparticles 5 times intra-peritoneally, respectively. Blood sampling was collected first day and 15 days after the last injection. Level of testosterone, FSH and LH were measured by ELISA method. After anesthesia and dissection of mice in each group, tissue sections of testis were prepared and stained with hematoxylin-eosin. Morphological status of spermatogenesis process and counting of types of cells (spermatogonium, spermatocyte and spermatid) were studied by optical microscope.
Results: In the first day of blood collection, a significant increase in LH and FSH level was observed at concentrations of 10 and 30 mg/kg, respectively. Also, Testosterone and FSH level decreased significantly reduced at 10 mg/kg/bw concentration compared to control (P<0.05). In 15 days after of the last injection, level of testosterone (P<0.05) and LH (P<0. 05) significantly increased in concentrations of 10 and 30 mg/kg/bw respectively. Also, there was a significant reduction in level of FSH in the concentration of 10 mg/kg/bw (P<0.05). The examination of testis tissue sections showed a significant decrease (P<0.05) in density and number of cell types (spermatogonium, spermatocyte and spermatid) and anomalies in the spermatogenesis process, in a dose-dependent manner. The most disturbances was seen at a concentration of 30 mg/kg/bw of copper oxide nanoparticles.
Conclusion: Copper oxide nanoparticles may interfere with the secretion of gonadotropins and testosterone and ultimately lead to a disruption of the spermatogenesis process.
Keywords: Copper oxide nanoparticles, Gonadotropins, Testosterone, Spermatogenesis
Full-Text [PDF 391 kb]   (8968 Downloads)    
Type of Study: Original Articles | Subject: Nanobiotecnology
References
1. Rubilar O, Rai M, Tortella G, Diez MC, Seabra AB, Durán N. Biogenic nanoparticles: copper, copper oxides, copper sulphides, complex copper nanostructures and their applications. Biotechnol Lett. 2013 Sep; 35(9): 1365-75. doi: 10.1007/s10529-013-1239-x
2. Azam A, Ahmed AS, Oves M, Khan MS, Memic A. Size-dependent antimicrobial properties of CuO nanoparticles against Gram-positive and -negative bacterial strains. Int J Nanomedicine. 2012; 7: 3527-35. doi: 10.2147/IJN.S29020
3. Sankar R, Maheswari R, Karthik S, Shivashangari KS, Ravikumar V. Anticancer activity of Ficus religiosa engineered copper oxide nanoparticles. Mater Sci Eng C Mater Biol Appl. 2014 Nov; 44: 234-39. doi: 10.1016/j.msec.2014.08.030
4. Seyedalipour B, Barimani N, Dehpour Jooybari A, Hosseini S M, Oshrieh M. Histopathological Evaluation of Kidney and Heart Tissues after Exposure to Copper Oxide Nanoparticles in Mus musculus. JBUMS. 2015; 17(7): 44-50. doi: 10.22088/jbums.17.7.44
5. Gomes T, Pereira CG, Cardoso C1, Pinheiro JP, Cancio I, Bebianno MJ. Accumulation and toxicity of copper oxide nanoparticles in the digestive gland of Mytilus galloprovincialis. Aquat Toxicol. 2012 Aug; 118-119: 72-79. doi: 10.1016/j.aquatox.2012.03.017
6. Jing X, Park JH, Peters TM, Thorne PS. Toxicity of copper oxide nanoparticles in lung epithelial cells exposed at the air-liquid interface compared with in vivo assessment. Toxicol In Vitro. 2015 Apr; 29(3): 502-11. doi: 10.1016/j.tiv.2014.12.023
7. Ahamed M, Akhtar MJ, Alhadlaq HA, Alrokayan SA. Assessment of the lung toxicity of copper oxide nanoparticles: current status. Nanomedicine (Lond). 2015; 10(15): 2365-77. doi: 10.2217/nnm.15.72
8. Lan Z, Yang WX. Nanoparticles and spermatogenesis: how do nanoparticles affect spermatogenesis and penetrate the blood-testis barrier. Nanomedicine (Lond). 2012 Apr; 7(4): 579-96. doi: 10.2217/nnm.12.20
9. Carlson C, Hussain SM, Schrand AM, Braydich-Stolle LK, Hess KL, Jones RL, et al. Unique cellular interaction of silver nanoparticles: size-dependent generation of reactive oxygen species. J Phys Chem B. 2008 Oct; 112(43): 13608-19. doi: 10.1021/jp712087m
10. Kalirawana TC, sharma P, Joshi SC. Reproductive Toxicity of Copper Nanoparticles in Male Albino Rats. Int J Pharma Res Health Sci. 2018; 6(1): 2258-63. doi: 10.21276/ijprhs.2018.01.29
11. Al Bairuty GA, Taha MN. Effects of copper nanoparticles on reproductive organs of male albino rats. International Journal for Sciences and Technology. 2016; 11(3): 17-24. doi: 10.12816/0034940
12. Thakur M, Gupta H, Singh D, Mohanty IR, Maheswari U, Vanage G, Joshi DS. Histopathological and ultra-structural effects of nanoparticles on rat testis following 90 days (Chronic study) of repeated oral administration. J Nanobiotechnology. 2014; 12: 42. doi: 10.1186/s12951-014-0042-8
13. Ostaszewska T, Chojnacki M, Kamaszewski M, Sawosz-Chwalibóg E. Histopathological effects of silver and copper nanoparticles on the epidermis, gills, and liver of Siberian sturgeon. Environ Sci Pollut Res Int. 2016 Jan; 23(2): 1621-33. doi: 10.1007/s11356-015-5391-9
14. Morgan AM, Abd El-Hamid MI, Noshy PA. Reproductive toxicity investigation of titanium dioxide nanoparticles in male albino rats. World Journal of Pharmacy and Pharmaceutical Sciences. 2015; 4(10): 34-49.
15. Zimmermann M. Ethical guidelines for investigations of experimental pain in conscious animals. Pain. 1983 Jun; 16(2): 109-10.
16. Doudi M, Setorki M. Acute effect of nano-copper on liver tissue and function in rat. Nanomedicine Journal. 2014; 1(5): 331-38. doi: 10.7508/NMJ.2015.05.007
17. Ema M, Okuda H, Gamo M, Honda K. A review of reproductive and developmental toxicity of silver nanoparticles in laboratory animals. Reprod Toxicol. 2017 Jan; 67: 149-64. doi: 10.1016/j.reprotox.2017.01.005
18. Seyedalipour B, Barimani N, Hoseini SM. [Embryonic malformations following exposure to copper oxide nanoparticles in Mus musculus]. J Shahrekord Univ Med Sci. 2015; 17(5): 23-32. [Article in Persian]
19. Seyedalipour B, Barimani N, Badoei-Dalfard A. [Evaluating of serum biochemical biomarker and liver histopathological changes in NMRI mice following exposure to copper oxide nanoparticle]. Razi j Med Sci . 2016; 23(5): 75-82. [Article in Persian]
20. Chattopadhyay A, Sarkar M, Biswas NM. Testosterone supplemented protection on inhibition of testicular function induced by copper chloride. DHR International Journal of Biomedical and Life Sciences (DHR-IJBLS). 2013; 4(1): 212-23.
21. Roychoudhury S, Nath S, Massanyi P, Stawarz R, Kacaniova M, Kolesarova A. Copper-induced changes in reproductive functions: in vivo and in vitro effects. Physiol Res. 2016; 65(1): 11-22.
22. Kochman K, Blitek A, Kaczmarek M, Gajewska A, Siawrys G, Counis R, et al. Different signaling in pig anterior pituitary cells by GnRH and its complexes with copper and nickel. Neuro Endocrinol Lett. 2005 Aug; 26(4): 377-82.
23. Chattopadhyay A, Sarkar M, Biswas NM. Effect of copper chloride on adrenocortical activities in adult and immature male rats. Environ Toxicol Pharmacol. 2002 Mar; 11(2): 79-84.
24. Lynn SE, Perfito N, Guardado D, Bentley GE. Food, stress, and circulating testosterone: Cue integration by the testes, not the brain, in male zebra finches (Taeniopygia guttata). Gen Comp Endocrinol. 2015 May; 215: 1-9. doi: 10.1016/j.ygcen.2015.03.010
25. Alarifi S, Ali D, Verma A, Alakhtani S, Ali BA. Cytotoxicity and genotoxicity of copper oxide nanoparticles in human skin keratinocytes cells. Int J Toxicol. 2013 Jul; 32(4): 296-307. doi: 10.1177/1091581813487563
26. Xu J, Li Z, Xu P, Xiao L, Yang Z. Nanosized copper oxide induces apoptosis through oxidative stress in podocytes. Arch Toxicol. 2013 Jun; 87(6): 1067-73. doi: 10.1007/s00204-012-0925-0
Send email to the article author


XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Rajabi S, Noori A, Shahbazi F. Copper oxide nanoparticles toxicity on pituitary gonadal axis and spermatogenesis in male rats. J Gorgan Univ Med Sci 2019; 21 (3) :49-56
URL: http://goums.ac.ir/journal/article-1-3438-en.html


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 21, Issue 3 (10-2019) Back to browse issues page
مجله دانشگاه علوم پزشکی گرگان Journal of Gorgan University of Medical Sciences
Persian site map - English site map - Created in 0.05 seconds with 38 queries by YEKTAWEB 4645