[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 21, Issue 1 (Spring 2019) ::
J Gorgan Univ Med Sci 2019, 21(1): 46-51 Back to browse issues page
Protective effect of melatonin and Amphotericin B in affected rats with Aspergillosis
Hossein Nowrozi * 1, Ali Kazemi2 , Mahsa Shokri3
1- Assistant Professor, Department of Laboratory Sciences, School of Allied Medical Sciences, Iran University of Medical Sciences, Tehran, Iran , nowrozi.h@iums.ac.ir
2- Assistant Professor, Department of Pharmacology, Science and Research Branch, Islamic Azad University, Tehran, Iran
3- Academic Instructor, Department of Microbiology, College of Medical Sciences, Varamin- Pishva Branch, Islamic Azad University, Varamin, Iran
Abstract:   (1741 Views)
Background and Objective: Fungal diseases are increasing in immunocompromised patients. One of the important fungal disseases is Aspergillosis. Few studies have been conducted on the effects of melatonin on fungal diseases. This study was performed to determine the protective effect of melatonin and Amphotericin B in Aspergillosis in rat model.
Methods: This experimental study was done on 40 Wistar rat with weight ranging from 200 to 250 grams. Animals were allocted in five groups. The first group was positive control (immunocompromised condition with cyclophosphamide and inoclusion of Aspergillus spore). The second groupe was negative control (without drug and inoclusion of Aspergillus spore) and the third group was treated by melatonin (200 μg/kg). The forth group was treated by Amphotericin B and fifth group was treated by melatonin plus Amphotericin B, IL.6 and TNFα were measured in the first and tenth days after contamination.
Results: The level of the IL.6 and TNFα in three interventional treated groups significantly reduced in compared to positive control group in first and tenth days (P<0.05). The level of the IL.6 and TNFα in melatonin plus Amphotericin B group significantly reduced in comparison with Amphotericin B and melatonin treated groups in first and tenth days (P<0.05).
Conclusion: Melatonin with Amphotericin B increases immune competent system in rats affected with Aspergillosis.
Keywords: Melatonin, Amphotericin B, Aspergillosis, IL.6, TNFα
Full-Text [PDF 240 kb]   (383 Downloads)    
Type of Study: Original Articles | Subject: Mycology
1. Tajik-Ijdan F, Kazemi A, Nowrozi H. Comparing the effects of alcoholic extract of ginseng with itraconazole against Candida albicans and Candida krusei. FEYZ. 2017; 21(3): 211-17.
2. Kazemi A, Nowrozi H, Badiee Moghadam M. [Drug susceptibility testing of clinical isolates of Candida albicans against Amphotericin B and Ketoconazole by microdilution and disk diffusion methods]. J Gorgan Univ Med Sci. 2017; 19(2): 59-64. [Article in Persian]
3. Wiederhold NP. Antifungal resistance: current trends and future strategies to combat. Infect Drug Resist. 2017 Aug; 10: 249-59. doi: 10.2147/IDR.S124918
4. Amstrup AK, Sikjaer T, Mosekilde L, Rejnmark L. The effect of melatonin treatment on postural stability, muscle strength, and quality of life and sleep in postmenopausal women: a randomized controlled trial. Nutr J. 2015 Sep; 14: 102. doi: 10.1186/s12937-015-0093-1
5. Aranda ML, González Fleitas MF, De Laurentiis A, Keller Sarmiento MI, Chianelli M, Sande PH, et al. Neuroprotective effect of melatonin in experimental optic neuritis in rats. J Pineal Res. 2016 Apr; 60(3): 360-72. doi: 10.1111/jpi.12318
6. Jenwitheesuk A, Nopparat C, Mukda S, Wongchitrat P, Govitrapong P. Melatonin regulates aging and neurodegeneration through energy metabolism, epigenetics, autophagy and circadian rhythm pathways. Int J Mol Sci. 2014 Sep; 15(9): 16848-84. doi: 10.3390/ijms150916848
7. Srinivasan V, Mohamed M, Kato H. Melatonin in bacterial and viral infections with focus on sepsis: a review. Recent Pat Endocr Metab Immune Drug Discov. 2012 Jan; 6(1): 30-39.
8. Yavuz T, Kaya D, Behçet M, Ozturk E, Yavuz Ö. Effects of melatonin on Candida sepsis in an experimental rat model. Advances in Therapy. 2007; 24(1): 91-100.
9. Clinical and Laboratory Standards Institute. Reference method for broth dilution antifungal susceptibility testing of filamentous fungi; approved standard. 2nd ed. CLSI document M38-A2. Clinical and Laboratory Standards Institute, Wayne, PA. 2008.
10. Vielma JR, Bonilla E, Chacín-Bonilla L, Mora M, Medina-Leendertz S, Bravo Y. Effects of melatonin on oxidative stress, and resistance to bacterial, parasitic, and viral infections: a review. Acta Trop. 2014 Sep; 137: 31-38. doi: 10.1016/j.actatropica.2014.04.021
11. Jiménez-Caliani AJ, Jiménez-Jorge S, Molinero P, Guerrero JM, Fernández-Santos JM, Martín-Lacave I, et al. Dual effect of melatonin as proinflammatory and antioxidant in collagen-induced arthritis in rats. J Pineal Res. 2005 Mar; 38(2): 93-99.
12. Rahman SA, Castanon-Cervantes O, Scheer FA, Shea SA, Czeisler CA, Davidson AJ, et al. Endogenous circadian regulation of pro-inflammatory cytokines and chemokines in the presence of bacterial lipopolysaccharide in humans. Brain Behav Immun. 2015 Jul; 47: 4-13. doi: 10.1016/j.bbi.2014.11.003
13. Cengiz MI, Cengiz S, Wang HL. Melatonin and oral cavity. Int J Dent. 2012; 2012: 491872. doi: 10.1155/2012/491872
14. Elmahallawy EK, Luque JO, Aloweidi AS, Gutiérrez-Fernández J, Sampedro-Martínez A, Rodriguez-Granger J, et al. Potential relevance of melatonin against some infectious agents: a review and assessment of recent research. Curr Med Chem. 2015; 22(33): 3848-61.
15. Yang HP, Tsang PC, Tsang PW. Melatonin inhibits biofilm formation in Candida parapsilosis. J Mycol Med. 2014 Dec; 24(4): 360-61. doi: 10.1016/j.mycmed.2014.05.003
16. Gómez-Moreno G, Guardia J, Ferrera MJ, Cutando A, Reiter RJ. Melatonin in diseases of the oral cavity. Oral Dis. 2010 Apr; 16(3): 242-47. doi: 10.1111/j.1601-0825.2009.01610.x
17. Terrón MP, Cubero J, Barriga C, Ortega E, Rodríguez AB. Phagocytosis of Candida albicans and superoxide anion Levels in ring dove (Streptopelia risoria) heterophils: effect of melatonin. J Neuroendocrinol. 2003 Dec; 15(12): 1111-15.
Send email to the article author

XML   Persian Abstract   Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Nowrozi H, Kazemi A, Shokri M. Protective effect of melatonin and Amphotericin B in affected rats with Aspergillosis. J Gorgan Univ Med Sci. 2019; 21 (1) :46-51
URL: http://goums.ac.ir/journal/article-1-3286-en.html

Volume 21, Issue 1 (Spring 2019) Back to browse issues page
مجله علمی دانشگاه علوم پزشکی گرگان Journal of Gorgan University of Medical Sciences
Persian site map - English site map - Created in 0.06 seconds with 31 queries by YEKTAWEB 4006