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:: Volume 20, Issue 1 (3-2018) ::
J Gorgan Univ Med Sci 2018, 20(1): 29-35 Back to browse issues page
Protective effect of perserved solutions of Krebs contains verapamil, adrenaline and propranololol in comparision with Krebs and Heparinated blood on desqumation of the endothelial cell in Saphenous vein of Guinea pig
Majid Malekzadeh Shafaroudi1 , Mitra Shokri2 , Zohre Zare1 , Alireza Rafiei3 , Mohammad Ali Ebrahimzadeh4 , Reza Moradpour5 , Nourollah Rezaei *6
1- Assistant Professor, Department of Anatomical Sciences, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
2- M.Sc Student of Anatomical Sciences, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
3- Professor, Cell and Molecular Research Center (CMRC), Department of Immunology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
4- Professor, Pharmaceutical Sciences Research Center (PSRC), Department of Medicinal Chemistry, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran
5- B.Sc Student of Medical Engineering, Department of Engineering, Rouzbehan Institute of Higher Education (College), Sari, Iran
6- Associate Professor, Cell and Molecular Research Center (CMRC), Department of Anatomical Sciences, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran. nourrezaei@gmail.com , nourrezaei@gmail.com
Keywords: Endothelium, Saphenous vein, Krebs solution, Verapamil, Nitric oxide, Guinea pig
Full-Text [PDF 333 kb] [English Abstract]   (18636 Downloads)     |   Abstract (HTML)  (29816 Views)
Type of Study: Original Articles | Subject: Histology
* Corresponding Author Address: Associate Professor, Cell and Molecular Research Center (CMRC), Department of Anatomical Sciences, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran. nourrezaei@gmail.com
Abstract:   (7627 Views)
Abstract
Background and Objective: The most important factor in the integrity of saphenous vein is the health degree of endothelium which guaranties the dilation of them after cronary bypass sergery. Kind of preservative soulution has a key role in endothelial protection. This study was done to evaluate Protective effect of perserved solutions of krebs contains verapamil, adrenaline and propranololol in comparision with Krebs and Heparinated blood on desqumation of the endothelial cell in Saphenous vein of Guinea pig.
Methods: This experimental study was done on 28 male Guinea pigs with 380±40g weight.for separating 3mm of saphenous vein rings and Measuring of rings nitric oxide released in preserving solutions: Krebs (K), Krebs plus propranolol (K+P), adrenaline (K+A) and verapamil (K+V) compaired with heparinized blood at 30, 45, 60 and 90 minutes after harvesting measured by micro plate Griess reaction. Rings also stained by H&E and examined by light microscopy to evaluate endothelial desqumation.
Results: Average concentration of nitric oxide (NO) in the Krebs plus Verapamil solution (K+V) Vs Heparinized Blood (HB), Krebs (K), Krebs plus Adrenaline (K+A) and Krebs plus Propanololol (K+P) revealed significant increase in NO release (P<0.05). The maximum NO measurement was 45 minuts after harvesting. Also histological study with H&E staining showed that endothelial layer was intact only in Krebs plus verapamil in compaired to control group, but in the other solutions the vascular intimal cells had suffered different degrees.
Conclusion: It seems that Krebs solutions containing verapamil has more efficiently to the proper functioning of the saphenous veins endothelium in animal modle.
Keywords: Endothelium, Saphenous vein, Krebs solution, Verapamil, Nitric oxide, Guinea pig
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Malekzadeh Shafaroudi M, Shokri M, Zare Z, Rafiei A, Ebrahimzadeh M A, Moradpour R et al . Protective effect of perserved solutions of Krebs contains verapamil, adrenaline and propranololol in comparision with Krebs and Heparinated blood on desqumation of the endothelial cell in Saphenous vein of Guinea pig. J Gorgan Univ Med Sci 2018; 20 (1) :29-35
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Volume 20, Issue 1 (3-2018) Back to browse issues page
مجله دانشگاه علوم پزشکی گرگان Journal of Gorgan University of Medical Sciences
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