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:: Volume 19, Issue 4 (12-2017) ::
J Gorgan Univ Med Sci 2017, 19(4): 100-105 Back to browse issues page
Evaluation of plasmid resistance to Mupirocin in Staphylococcus aureus strains isolated from clinical specimens of the skin of hospital employees and hospitalized patients
Maral Khamehchi1 , Mohammad Reza Mehrabi2 , Reza Yari 3
1- M.Sc in Cell Biology, Department of Biology, Faculty of Sciences, Borujerd Branch, Islamic Azad University, Borujerd, Iran
2- Assistant Professor of Hematology and Blood Banking, Department of Laboratory Sciences, Medical Faculty, Borujerd Branch, Islamic Azad University, Borujerd, Iran
3- Assistant Professor of Cell Biology, Department of Biology, Faculty of Sciences, Borujerd Branch, Islamic Azad University, Borujerd, Iran , rezayari@yahoo.com
Abstract:   (11123 Views)
Background and Objective: Mupirocin is a secreted antibiotic inhibitor of Isoleucine-tRNA, a bacterial synthetase that is used against yellow wounds from Streptococcus pyogenic and Staphylococcus aureus. This study was carried out to determine the plasmid resistance of mupirocin in Staphylococcus aureus strains isolated from clinical specimens of the skin of hospital employees and hospitalized patients.
Methods: This descriptive study was performed on 150 strains of Staphylococcus aureus isolated from clinical specimens of the skin of patients and employees of three hospitals in Qom, Iran during
2014-15. In order to confirm the identity of Staphylococcus aureus isolates, conventional biochemical methods were used. Also, PCR of srRNA16 was used for molecular confirmation of isolates. The presence of mupA (iles-2) and mupB plasmid genes was investigated using PCR method and AluI enzyme digestion plan was performed for them. Disc diffusion method was used to demonstrate resistance to mupirocin.
Results: Seven isolated samples (4.66%) were resistant to mupirocin. All Mupirocin-resistant isolates possessed PCR-positive mupacysin mupirocidal genes (iles-2) and mupB, and all plasmid genes were resistant to all resistant specimens. Genotyping of mupB gene was able to isolate samples from patients and staff as well as male and female.
Conclusion: The prevalence of mupirocin-resistant Staphylococcus aureus isolated from skin specimens was low.
Keywords: Staphylococcus aureus, Plasmid, Mupirocin, Skin, Patient
Full-Text [PDF 313 kb] [English Abstract]   (14310 Downloads) |   |   Abstract (HTML)  (796 Views)  
Type of Study: Original Articles | Subject: Microbiology
References
1. Hesami S, Hosseini SD, Amouzandeh-Nobaveh A, Eskandari S, Ghaznavi-Rad E. [Phenotypic and genotypic determination of mupirocin resistance among Methicillin susceptibility and resistance in Staphylococci from nosocomial infections]. J Mazandaran Univ Med Sci. 2014; 23(1): 30-39. [Article in Persian]
2. Rahimi-Alang S, Asmar M, Cheraghali F, Yazarlou S, Amini A, Shakeri F, et al. [Frequency of Methicillin resistant Staphylococcus aureus in health care]. Zahedan J Res Med Sci. 2011; 13(1): 17-22. [Article in Persian]
3. Teyhoo M, Mobin H, Mozafari N A, Moadab S R, Sedigh Bayan KH, Mones Rast SH. The prevalence of toxin shock syndrome oxin (TSST-1) producing clinical isolates of Staphylococcus aureus Strains isolated from Shohada Hospital in Tabriz, Iran. Med Lab J. 2011; 5(1): 38-44. [Article in Persian]
4. Darabi N, Habibollahi H, Shahbabian K. [Molecular epidemiology of Staphylococcus aureus isolated from patients and personnel in army hospital]. Ann Mil Health Sci Res. 2010; 8(3): 193-99. [Article in Persian]
5. Rahimi F, Bouzari M, Katoli M, Pourshafi MR. [The prevalence Methicillin-resistant Staphylococcus aureus strains produce enterotoxin A in Tehran hospitals]. Iran J Infect Dis Trop Med. 2014; 19(65): 59-68. [Article in Persian]
6. Saderi H, Owlia P, Habibi M. [Detection of resistance to mupirocin in Staphylococcus aureus Strains isolated from patients in four University Hospitals of Tehran by Polymerase Chain Reaction (PCR) method]. Daneshvar Med. 2009; 16(78): 31-38. [Article in Persian]
7. Ramsey MA, Bradley SF, Kauffman CA, Morton TM. Identification of chromosomal location of mupA gene, encoding low-level mupirocin resistance in staphylococcal isolates. Antimicrob Agents Chemother. 1996 Dec; 40(12): 2820-23.
8. Udo EE, Jacob LE, Mathew B. Genetic analysis of methicillin-resistant Staphylococcus aureus expressing high- and low-level mupirocin resistance. J Med Microbiol. 2001 Oct; 50(10): 909-15. doi:10.1099/0022-1317-50-10-909
9. Seah C, Alexander DC, Louie L, Simor A, Low DE, Longtin J, et al. MupB, a new high-level mupirocin resistance mechanism in Staphylococcus aureus. Antimicrob Agents Chemother. 2012 Apr; 56(4): 1916-20. doi:10.1128/AAC.05325-11
10. Yun HJ, Lee SW, Yoon GM, Kim SY, Choi S, Lee YS, et al. Prevalence and mechanisms of low- and high-level mupirocin resistance in staphylococci isolated from a Korean hospital. J Antimicrob Chemother. 2003 Mar; 51(3): 619-23.
11. Godbeer SM, Gold RM, Lawhon SD. Prevalence of mupirocin resistance in Staphylococcus pseudintermedius. J Clin Microbiol. 2014 Apr; 52(4): 1250-52. doi:10.1128/ JCM.03618-13
12. Ghobadi Nejad M. [Antimicrobial multi-resistance Staphylococci and gene transferring in bacteria]. JBUMS. 2003; 5(1): 55-67. [Article in Persian]
13. McNeil JC, Hulten KG, Kaplan SL, Mason EO. Mupirocin resistance in Staphylococcus aureus causing recurrent skin and soft tissue infections in children. Antimicrob Agents Chemother. 2011 May; 55(5): 2431-33. doi:10.1128/AAC.01587-10
14. Farahani N, Mirnejad R, Ahmadi Z, Amirmozafari N, Masjedian F. [Molecular typing of Acinetobacter Baumannii clinical strains in Tehran by Pulsed-Field Gel Electrophoresis]. J Fasa Univ Med Sci. 2013; 2(4): 259-65. [Article in Persian]
15. Khalafian H, Momtaz H. [Typing of the Shiga toxin – producing Escherichia coli strains isolated from diarrheic children using Multi Locus Sequence Typing (MLST)]. Iran J Med Microbiol. 2015; 9(1): 14-21. [Article in Persian]
16. Reisi M, Tajbakhsh E, Momtaz H. [Coagulase gene polymorphism of Staphylococcus aureus isolated from clinical respiratory system infections in Shahrekord]. Journal of Microbial World. 2014; 7(3): 206-13. [Article in Persian]
17. Ranjbar R, Mirzaie A, Sadeghifard N, Jonaidi N. [Molecular typing of Salmonella infantis clinical strains isolated in Tehran]. J Mil Med. 2014; 16(1): 17-22. [Article in Persian]
18. Zaker Bostanabad S, Jabbarzadeh E, Pourazar S, Ghalami M. [Typing of Mycobacterium tuberculosis isolated from patients in the Tehran city by Spoilgotyping]. New cellular and molecular biotechnology journal. 2011; 1(2): 55-60. [Article in Persian]
19. Rafee Tabatabaei R, Pourbakhsh SA, Jalali Z. [The study of different methods of typing for diagnosis and differentiation of E. coli strains isolated from urinary tract infections]. Journal of Sciences (Islamic Azad University). 2010; 19(1): 1-14. [Article in Persian]
20. Shokoohizadeh L. Molecular methods for bacterial strain typing. Med Lab J. 2016; 10(2): 1-7.
21. Alder J, Farraro MJ, Hindler JA, Patel JB, Zimmer BL, Hecht DW, et al. Performance standards for antimicrobial susceptibility testing; twenty-third informational supplement. Clinical and Laboratory Standards Institute. 2013; 33(1): 3-76.
22. McFarland J. Nephelometer: An instrument for estimating the number of bacteria in suspensions used for calculating the opsonic index and for vaccines. JAMA. 1907; 14: 1176-78. doi:10.1001/jama.1907.25320140022001f
23. Kaboosi H, Khandan Del A, Ghaemi E A, Bakhshande Nosrat S, Ayatollahi AA, Golriz N. Effect of mupirocin treatment in eradication of Staphylococcus aureus nasal carriage among intensive care unit personnel. Med Lab J. 2016; 10(2): 14-18.
24. Peerayeh ShN, Esmaeili D. [Antibiotic efflux pumps]. Ann Mil Health Sci Res. 2004; 2(1): 301-6. [Article in Persian]
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Khamehchi M, Mehrabi M R, Yari R. Evaluation of plasmid resistance to Mupirocin in Staphylococcus aureus strains isolated from clinical specimens of the skin of hospital employees and hospitalized patients. J Gorgan Univ Med Sci 2017; 19 (4) :100-105
URL: http://goums.ac.ir/journal/article-1-3210-en.html


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Volume 19, Issue 4 (12-2017) Back to browse issues page
مجله دانشگاه علوم پزشکی گرگان Journal of Gorgan University of Medical Sciences
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