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:: Volume 18, Issue 3 (10-2016) ::
J Gorgan Univ Med Sci 2016, 18(3): 86-91 Back to browse issues page
Minimum inhibitory concentration of silver nanoparticle against Staphylococcus aureus and its relation with Methicillin resistance and bacterial source of isolation
F Azadi1 , A Jamali2 , B Baei3 , M Bazouri4 , F Shakeri5 , E Ghaemi * 6
1- M.Sc Student in Medical Microbiology, Faculty of Medicine, Golestan University of Medical Sciences, Gorgan, Iran.
2- Assistant Professor, Medical Laboratory Research Center, Golestan University of Medical Sciences, Gorgan, Iran.
3- M.Sc Student in Microbiology, Amol Branch, Islamic of Azad University, Amol, Iran.
4- B.Sc in Laboratory Sciences, Faculty of Medicine, Golestan University of Medical Sciences, Gorgan, Iran.
5- M.Sc in Microbiology, Faculty of Medicine, Golestan University of Medical Sciences, Gorgan, Iran.
6- Professor, Medical Laboratory Research Center,Golestan University of Medical Sciences,Gorgan, Iran. , eghaemi@yahoo.com
Abstract:   (12579 Views)

Background and Objective: The rise of antibiotic resistance particulary Methicillin resistance in pathogenic bacteria such as Staphylococcus aureus is found to be an emerging threat to human health especially in hospitals. Heavy metal nanoparticles such as Ag used for inhibition of this bacterium. This study was done to determine of minimum inhibitory concentration (MIC) Ag nanoparticle against Staphylococcus aureus which isolated in Gorgan, north of Iran and its relation with Methicillin resistance and source of bacteria.

Methods: In this descriptive – analytical study, the MIC Ag nanoparticle in 183 isolates of Staphylococcus aureus by microdilution method was determined. 30 isolates, based on mecA gene was considered as MRSA. Samples were collected from patients, nose of healthy carriers and foods. Compare the MIC of isolates based on Methicillin resistance, source of the bacteria and resistance to other antibiotics were assessed.

Results: Out of 183 samples MIC was varied from 1 to 16 µg/ml, and mean±std was 2.9±1.89 µg/ml. MIC mean of silver nanoparticles in isolated from foods were 2±0.7, isolared from healthy carriers were 4.1±2.4 and from patients were 3.4±2.1 µg/ml and were statically significant (P<0.05). MIC mean of silver nanoparticles in MSSA isolates are 3.9±2.3 and in MRSA isolates are 2.4±1.4 µg/ml that were statically significant (P<0.05). MIC mean of gentamycin resistant isolate were lower than sensitive one. But between MIC of silver nanoparticles and other antibiotics resistance was not significant statistically.

Conclusion: There is a relation between silver nanoparticle MIC, source of sample isolation, Methicillin and gentamycin resistance. Since MIC of silver nanoparticles on isolates of Methicillin resistant is low, the possibility of its use in the control of MRSA in hospital infections can be considered as a prime attention the Gentamycine.
Keywords: Staphylococcus aureus, Silver nanoparticle, Minimum inhibitory concentration, MecA gene
Full-Text [PDF 396 kb] [English Abstract]   (14953 Downloads) |   |   Abstract (HTML)  (1583 Views)  
Type of Study: Original Articles | Subject: Microbiology
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Azadi F, Jamali A, Baei B, Bazouri M, Shakeri F, Ghaemi E. Minimum inhibitory concentration of silver nanoparticle against Staphylococcus aureus and its relation with Methicillin resistance and bacterial source of isolation. J Gorgan Univ Med Sci 2016; 18 (3) :86-91
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Volume 18, Issue 3 (10-2016) Back to browse issues page
مجله دانشگاه علوم پزشکی گرگان Journal of Gorgan University of Medical Sciences
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