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The Antioxidant Effect of Apigenin on Human Sperm Quality after Freezing-Thawing
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Asieh Esmaeili Irani1    , Bahare Nikoozar2 , Maryam Arbabian3 , Marziyeh Tavalaee * 4, Mohammad Hossein Nasr-Esfahani5 |
1- M.Sc Student of Microbial Biotechnology, Department of Animal Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran. Isfahan Fertility and Infertility Center, Isfahan, Iran.
2- M.Sc in Microbial Biotechnology, Department of Animal Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran.
3- B.Sc in Cellular and Molecular Biology, Department of Animal Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran.
4- Associate Professor, Department of Animal Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran. , m.tavalaee@royan-rc.ac.ir
5- Professor, Department of Animal Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran. |
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Abstract: (1546 Views) |
Background and Objective: Antioxidant apigenin (AP) is a natural, non-mutagenic, and less toxic flavonoid with pharmacological anti-cancer, anti-viral, anti-bacterial, anti-apoptotic, and anti-inflammatory activities. This antioxidant is easily received by the cell, binds to sperm DNA, and forms a DNA-AP complex, thereby protecting sperm DNA. The present study was conducted to determine the antioxidant effect of AP on human sperm quality after freezing-thawing.
Methods: In this descriptive-analytical study, 10 normozoospermic samples underwent freezing-thawing conditions, and sperm functional tests were investigated in different AP concentrations, including 0.4 mM, 0.2 mM, 0.1 mM, and 0.05 mM.
Results: The quality of total sperm parameters and functional tests decreased after freezing compared to before freezing. Among the AP concentrations, only in the 0.2 mM AP concentration, the improvement of the additional histone percentage, protamine deficiency, and sperm DNA health were observed compared to the control; this finding was not statistically significant.
Conclusion: The use of AP with a concentration of 0.2 mM during freezing-thawing culminates in improving sperm functional tests. |
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Keywords: Oxidative Stress [MeSH], Antioxidants [MeSH], Apigenin [MeSH], Spermatozoa [MeSH], Freeze-Thaw
Article ID: Vol26-08 |
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Full-Text [PDF 1084 kb]
(4250 Downloads)
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Type of Study: Original Articles |
Subject:
Reproductive Biology
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1. Liu S, Li F. Cryopreservation of single-sperm: where are we today? Reprod Biol Endocrinol. 2020 May 12;18(1):41. doi: 10.1186/s12958-020-00607-x. [ DOI] [ PubMed] 2. Polge C, Smith AU, Parkes AS. Revival of spermatozoa after vitrification and dehydration at low temperatures. Nature. 1949 Oct 15;164(4172):666. doi: 10.1038/164666a0. [ DOI] [ PubMed] 3. Sherman JK. Improved methods of preservation of human spermatozoa by freezing and freeze-drying. Fertil Steril. 1963 Jan-Feb;14:49-64. doi: 10.1016/s0015-0282(16)34746-x. [ DOI] [ PubMed] 4. Clarke GN, Liu DY, Baker HW. Improved sperm cryopreservation using cold cryoprotectant. Reprod Fertil Dev. 2003;15(7-8):377-81. doi: 10.1071/RD03007. [ DOI] [ PubMed] 5. Ping P, Zhu WB, Zhang XZ, Yao KS, Xu P, Huang YR, et al. Sperm banking for male reproductive preservation: a 6-year retrospective multi-centre study in China. Asian J Androl. 2010 May;12(3):356-62. doi: 10.1038/aja.2010.12. [ DOI] [ PubMed] 6. Hezavehei M, Sharafi M, Kouchesfahani HM, Henkel R, Agarwal A, Esmaeili V, et al. Sperm cryopreservation: A review on current molecular cryobiology and advanced approaches. Reprod Biomed Online. 2018 Sep;37(3):327-339. doi: 10.1016/j.rbmo.2018.05.012. [ DOI] [ PubMed] 7. Bai H, Zhang Y, Tian S, Hu R, Liang Y, Gao J, Wang Y, Wu B. Elamipretide as a potential candidate for relieving cryodamage to human spermatozoa during cryopreservation. Cryobiology. 2020 Aug;95:138-142. doi: 10.1016/j.cryobiol.2020.03.011. [ DOI] [ PubMed] 8. Said TM, Gaglani A, Agarwal A. Implication of apoptosis in sperm cryoinjury. Reprod Biomed Online. 2010 Oct;21(4):456-62. doi: 10.1016/j.rbmo.2010.05.011. [ DOI] [ PubMed] 9. Chavoshi Nezhad N, Vahabzadeh Z, Allahveisie A, Rahmani K, Raoofi A, Rezaie MJ, et al. The Effect of L-Carnitine and Coenzyme Q10 on the Sperm Motility, DNA Fragmentation, Chromatin Structure and Oxygen Free Radicals During, before and after Freezing in Oligospermia Men. Urol J. 2021 Feb 6;18(3):330-336. doi: 10.22037/uj.v16i7.6400. [ DOI] [ PubMed] 10. Li Z, Lin Q, Liu R, Xiao W, Liu W. Protective effects of ascorbate and catalase on human spermatozoa during cryopreservation. J Androl. 2010 Sep-Oct;31(5):437-44. doi: 10.2164/jandrol.109.007849. [ DOI] [ PubMed] 11. Kalthur G, Raj S, Thiyagarajan A, Kumar S, Kumar P, Adiga SK. Vitamin E supplementation in semen-freezing medium improves the motility and protects sperm from freeze-thaw-induced DNA damage. Fertil Steril. 2011 Mar 1;95(3):1149-51. doi: 10.1016/j.fertnstert.2010.10.005. [ DOI] [ PubMed] 12. Yan X, Qi M, Li P, Zhan Y, Shao H. Apigenin in cancer therapy: anti-cancer effects and mechanisms of action. Cell Biosci. 2017 Oct 5;7:50. doi: 10.1186/s13578-017-0179-x. [ DOI] [ PubMed] 13. Sharma H, Kanwal R, Bhaskaran N, Gupta S. Plant flavone apigenin binds to nucleic acid bases and reduces oxidative DNA damage in prostate epithelial cells. PLoS One. 2014 Mar 10;9(3):e91588. doi: 10.1371/journal.pone.0091588. [ DOI] [ PubMed] 14. Akilah A, Balaha M, Abd-El Rahman MN, Hedya S. Apigenin and Baicalin, Each Alone or in Low Dose Combination, Attenuated Chloroquine Induced Male Infertility in Adult Rats. Thai Journal of Pharmaceutical Sciences (TJPS). 2018 Aug; 42(3): 118-28. [ Link] 15. Pei Y, Yang L, Wu L, He H, Geng G, Xu D, Chen H, Li Q. Combined effect of apigenin and ferulic acid on frozen-thawed boar sperm quality. Anim Sci J. 2018 Jul;89(7):956-965. doi: 10.1111/asj.13009. [ DOI] [ PubMed] 16. World Health Organization. WHO laboratory manual for the examination and processing of human semen. 6th ed. 2021. [ Link] 17. Aitken RJ, Wingate JK, De Iuliis GN, McLaughlin EA. Analysis of lipid peroxidation in human spermatozoa using BODIPY C11. Mol Hum Reprod. 2007 Apr;13(4):203-11. doi: 10.1093/molehr/gal119. [ DOI] [ PubMed] 18. Kiani-Esfahani A, Tavalaee M, Deemeh MR, Hamiditabar M, Nasr-Esfahani MH. DHR123: an alternative probe for assessment of ROS in human spermatozoa. Syst Biol Reprod Med. 2012 Jun;58(3):168-74. doi: 10.3109/19396368.2012.681420. [ DOI] [ PubMed] 19. Hamilton TRDS, Assumpção MEOD. Sperm DNA fragmentation: causes and identification. Zygote. 2020 Feb;28(1):1-8. doi: 10.1017/S0967199419000595. [ DOI] [ PubMed] 20. Jesse JP, Dhawan V, Dada R. Chromatin Condensation: Aniline Blue Stain. In: Agarwal A, Henkel R, Majzoub A. Manual of Sperm Function Testing in Human Assisted Reproduction. Cambridge: Cambridge University Press. 2021; pp: 142-50. [ Link] [ DOI] 21. Evenson D. DNA Damage: Sperm Chromatin Structure Assay: Sperm Chromatin Structure Assay Test on its Fortieth Anniversary. In: Agarwal A, Henkel R, Majzoub A, editors. Manual of Sperm Function Testing in Human Assisted Reproduction. Cambridge: Cambridge University Press. 2021; pp: 192-201. doi: 10.1017/9781108878715.023. [ DOI] 22. Martinez-Soto JC, de DiosHourcade J, Gutiérrez-Adán A, Landeras JL, Gadea J. Effect of genistein supplementation of thawing medium on characteristics of frozen human spermatozoa. Asian J Androl. 2010 May;12(3):431-41. doi: 10.1038/aja.2009.92. [ DOI] [ PubMed] 23. Thomson LK, Fleming SD, Aitken RJ, De Iuliis GN, Zieschang JA, Clark AM. Cryopreservation-induced human sperm DNA damage is predominantly mediated by oxidative stress rather than apoptosis. Hum Reprod. 2009 Sep;24(9):2061-70. doi: 10.1093/humrep/dep214. [ DOI] [ PubMed] 24. Silvestre MA, Yániz JL, Peña FJ, Santolaria P, Castelló-Ruiz M. Role of Antioxidants in Cooled Liquid Storage of Mammal Spermatozoa. Antioxidants (Basel). 2021 Jul 8;10(7):1096. doi: 10.3390/antiox10071096. [ DOI] [ PubMed] 25. Bahmyari R, Zare M, Sharma R, Agarwal A, Halvaei I. The efficacy of antioxidants in sperm parameters and production of reactive oxygen species levels during the freeze-thaw process: A systematic review and meta-analysis. Andrologia. 2020 Apr;52(3):e13514. doi: 10.1111/and.13514. [ DOI] [ PubMed] 26. Zou YJ, Yang J, Chang S, Xu WM, Yin TL, Long W. Acetyl-L-carnitine: An effective antioxidant against cryo-damage on human spermatozoa with asthenospermia. J Huazhong Univ Sci Technolog Med Sci. 2017 Dec;37(6):915-921. doi: 10.1007/s11596-017-1827-4. [ DOI] [ PubMed] 27. Rossi T, Mazzilli F, Delfino M, Dondero F. Improved human sperm recovery using superoxide dismutase and catalase supplementation in semen cryopreservation procedure. Cell Tissue Bank. 2001;2(1):9-13. doi: 10.1023/A:1011592621487. [ DOI] [ PubMed] 28. Kalthur G, Raj S, Thiyagarajan A, Kumar S, Kumar P, Adiga SK. Vitamin E supplementation in semen-freezing medium improves the motility and protects sperm from freeze-thaw-induced DNA damage. Fertil Steril. 2011 Mar 1;95(3):1149-51. doi: 10.1016/j.fertnstert.2010.10.005. [ DOI] [ PubMed] 29. Mohammadi F, Varanloo N, Heydari Nasrabadi M, Vatannejad A, Amjadi FS, Javedani Masroor M, et al. Supplementation of sperm freezing medium with myoinositol improve human sperm parameters and protects it against DNA fragmentation and apoptosis. Cell Tissue Bank. 2019 Mar;20(1):77-86. doi: 10.1007/s10561-018-9731-0. [ DOI] [ PubMed] 30. Najafi L, Halvaei I, Movahedin M. Canthaxanthin protects human sperm parameters during cryopreservation. Andrologia. 2019 Nov;51(10):e13389. doi: 10.1111/and.13389. [ DOI] [ PubMed] |
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Esmaeili Irani A, Nikoozar B, Arbabian M, Tavalaee M, Nasr-Esfahani M H. The Antioxidant Effect of Apigenin on Human Sperm Quality after Freezing-Thawing. J Gorgan Univ Med Sci 2024; 26 (1) :77-86 URL: http://goums.ac.ir/journal/article-1-4329-en.html
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