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The Antioxidant Effect of Apigenin on Human Sperm Quality after Freezing-Thawing
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Asieh Esmaeili Irani1    , Bahare Nikoozar2 , Maryam Arbabian3 , Marziyeh Tavalaee * 4, Mohammad Hossein Nasr-Esfahani5 |
1- M.Sc Student of Microbial Biotechnology, Department of Animal Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran. Isfahan Fertility and Infertility Center, Isfahan, Iran.
2- M.Sc in Microbial Biotechnology, Department of Animal Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran.
3- B.Sc in Cellular and Molecular Biology, Department of Animal Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran.
4- Associate Professor, Department of Animal Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran. , m.tavalaee@royan-rc.ac.ir
5- Professor, Department of Animal Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran. |
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Abstract: (1588 Views) |
Background and Objective: Antioxidant apigenin (AP) is a natural, non-mutagenic, and less toxic flavonoid with pharmacological anti-cancer, anti-viral, anti-bacterial, anti-apoptotic, and anti-inflammatory activities. This antioxidant is easily received by the cell, binds to sperm DNA, and forms a DNA-AP complex, thereby protecting sperm DNA. The present study was conducted to determine the antioxidant effect of AP on human sperm quality after freezing-thawing.
Methods: In this descriptive-analytical study, 10 normozoospermic samples underwent freezing-thawing conditions, and sperm functional tests were investigated in different AP concentrations, including 0.4 mM, 0.2 mM, 0.1 mM, and 0.05 mM.
Results: The quality of total sperm parameters and functional tests decreased after freezing compared to before freezing. Among the AP concentrations, only in the 0.2 mM AP concentration, the improvement of the additional histone percentage, protamine deficiency, and sperm DNA health were observed compared to the control; this finding was not statistically significant.
Conclusion: The use of AP with a concentration of 0.2 mM during freezing-thawing culminates in improving sperm functional tests. |
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Keywords: Oxidative Stress [MeSH], Antioxidants [MeSH], Apigenin [MeSH], Spermatozoa [MeSH], Freeze-Thaw
Article ID: Vol26-08 |
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Full-Text [PDF 1084 kb]
(4348 Downloads)
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Type of Study: Original Articles |
Subject:
Reproductive Biology
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Esmaeili Irani A, Nikoozar B, Arbabian M, Tavalaee M, Nasr-Esfahani M H. The Antioxidant Effect of Apigenin on Human Sperm Quality after Freezing-Thawing. J Gorgan Univ Med Sci 2024; 26 (1) :77-86 URL: http://goums.ac.ir/journal/article-1-4329-en.html
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