|
Effect of Silver Nanoparticles on the Kidney Tissues of Quercetin-Treated NMRI Rats: A Stereological Study
|
Seyed Mohammad Ali Shariatzadeh *    1, Zahra Soori2 , Parisa Maleki3 |
1- Professor, Department of Biology, Faculty of Science, Arak University, Arak, Iran. , s-shariatzadeh@araku.ac.ir
2- M.Sc in Biology, Department of Biology, Faculty of Science, Arak University, Arak, Iran.
3- M.Sc in Biology, Academic Instructor, Department of Biology, Faculty of Science, Arak University, Arak, Iran. |
|
|
Abstract: (1929 Views) |
Background and Objective: Considering the increasing use of silver nanoparticles in various products, including industrial and medical products, serious worries have been created regarding the potential dangers of silver nanoparticles. This study was conducted to determine the effect of silver nanoparticles on the kidney tissues of quercetin-treated NMRI rats.
Methods: In this experimental study, 24 adult male NMRI rats were randomly divided into 4 groups of 6. The groups included the control group, the silver nanoparticles group (500 mg/kg/bw), the quercetin group (50 mg/kg/bw), and the silver nanoparticles (500 mg/kg/bw) + quercetin (50 mg/kg/bw) group. Silver nanoparticles were fed orally on a daily basis for 35 days. Quercetin was injected intraperitoneally on a daily basis for 42 days. At the end of the study, after taking blood from the rats, the dissection, tissue passaging, and Heidenhain’s Azan staining stages were carried out. The total volumes of the kidney, cortex and medulla, renal corpuscle, and glomerulus were evaluated by a stereological method. A qualitative assessment of apoptotic cells was performed using the tunnel method. The amount of malondialdehyde (MDA) in blood serum was specified as an indicator of lipid peroxidation by the Buege and Aust method.
Results: Comparing the body weight and kidneys, and the total kidney, cortex, and medulla volumes showed no statistically significant difference between the silver nanoparticles group and the control group. The silver nanoparticles group showed a significant increase in the total mean renal corpuscle volume, glomerular volume, tuft volume, Bowman’s capsule membrane volume, and the amount of MDA compared to the control group (P<0.05). Also, a statistically significant reduction was observed in the silver nanoparticles group in the total mean volume of Bowman’s capsule and capillary spaces compared to the control group (P<0.05). Quercetin could reduce the detrimental effects of silver nanoparticles on kidney cells as much as the control group; however, apoptosis was not shown in kidney cells in the group treated with quercetin. Assessing the cells in the silver nanoparticles group indicated the creation of apoptosis. The amount of serum MDA in the silver nanoparticles group showed a statistically significant increase compared to other groups (P<0.05).
Conclusion: The results of this study demonstrated that quercetin could reduce the detrimental effects of silver nanoparticles on kidney cells as much as the control group. |
|
Keywords: Nanoparticles [MeSH], Silver [MeSH], Quercetin [MeSH], Kidney [MeSH], Mice [MeSH]
Article ID: Vol25-42 |
|
|
Full-Text [PDF 1054 kb]
(4975 Downloads)
|
|
Type of Study: Original Articles |
Subject:
Nanobiotecnology
|
|
|
|
|
|
|
| References |
1. Asare N, Instanes C, Sandberg WJ, Refsnes M, Schwarze P, Kruszewski M, et al. Cytotoxic and genotoxic effects of silver nanoparticles in testicular cells. Toxicology. 2012 Jan; 291(1-3): 65-72. doi: 10.1016/j.tox.2011.10.022. [ DOI] [ PubMed] 2. Martirosyan A, Bazes A, Schneider YJ. In vitro toxicity assessment of silver nanoparticles in the presence of phenolic compounds--preventive agents against the harmful effect? Nanotoxicology. 2014 Aug; 8(5): 573-82. doi: 10.3109/17435390.2013.812258. [ DOI] [ PubMed] 3. Adeyemi OS, Faniyan TO. Antioxidant status of rats administered silver nanoparticles orally. Journal of Taibah University Medical Sciences. 2014 Sep; 9(3): 182-86. doi: 10.1016/j.jtumed.2014.03.002. [ Link] [ DOI] 4. Nabinejad A, Noaman V, Khayyam Nekouiee M. Evaluation of silver residues accumulation in tissues of Broilers treated with nanosilver using MNSR (A Clinical Trial). Archives of Razi Institute. 2016; 71(1): 51-55. doi: 10.22034/ari.2016.105998. [ View at Publisher] [ DOI] 5. Völker C, Oetken M, Oehlmann J. The biological effects and possible modes of action of nanosilver. Rev Environ Contam Toxicol. 2013; 223: 81-106. doi: 10.1007/978-1-4614-5577-6_4. [ DOI] [ PubMed] 6. Basile P, Greengard E, Weigel B, Spector L. Prognostic Factors for Development of Subsequent Metastases in Localized Osteosarcoma: A Systematic Review and Identification of Literature Gaps. Sarcoma. 2020 Mar; 2020: 7431549. doi: 10.1155/2020/7431549. [ DOI] [ PubMed] 7. García-Saura MF, Galisteo M, Villar IC, Bermejo A, Zarzuelo A, Vargas F, et al. Effects of chronic quercetin treatment in experimental renovascular hypertension. Mol Cell Biochem. 2005 Feb; 270(1-2): 147-55. doi: 10.1007/s11010-005-4503-0. [ DOI] [ PubMed] 8. Abdel-Raheem IT, Abdel-Ghany AA, Mohamed GA. Protective effect of quercetin against gentamicin-induced nephrotoxicity in rats. Biol Pharm Bull. 2009 Jan; 32(1): 61-67. doi: 10.1248/bpb.32.61. [ DOI] [ PubMed] 9. Biazzo A, De Paolis M. Multidisciplinary approach to osteosarcoma. Acta Orthop Belg. 2016 Dec; 82(4): 690-98. [ PubMed] 10. Sarhan OM, Hussein RM. Effects of intraperitoneally injected silver nanoparticles on histological structures and blood parameters in the albino rat. Int J Nanomedicine. 2014 Mar; 9: 1505-17. doi: 10.2147/IJN.S56729. [ DOI] [ PubMed] 11. Behling EB, Sendão MC, Francescato HD, Antunes LM, Costa RS, Bianchi Mde L. Comparative study of multiple dosage of quercetin against cisplatin-induced nephrotoxicity and oxidative stress in rat kidneys. Pharmacol Rep. 2006 Jul-Aug; 58(4): 526-32. [ PubMed] 12. Murmu S, Shrivastava VK. Protective Action of an Anti-oxidant (Vitamin-C) Against Bisphenol-toxicity in Cirrhinus mrigala (Ham.). Turkish Journal of Fisheries and Aquatic Sciences. 2011; 11: 5-29. doi: 10.4194/trjfas.2011.0104. [ View at Publisher] [ DOI] 13. Rahman MF, Wang J, Patterson TA, Saini UT, Robinson BL, Newport GD, et al. Expression of genes related to oxidative stress in the mouse brain after exposure to silver-25 nanoparticles. Toxicol Lett. 2009 May; 187(1): 15-21. doi: 10.1016/j.toxlet.2009.01.020. [ DOI] [ PubMed] 14. Hoseini L, Roozbeh J, Sagheb M, Karbalay-Doust S, Noorafshan A. Nandrolone decanoate increases the volume but not the length of the proximal and distal convoluted tubules of the mouse kidney. Micron. 2009 Feb; 40(2): 226-30. doi: 10.1016/j.micron.2008.08.004. [ DOI] [ PubMed] 15. Buege JA, Aust SD. Microsomal lipid peroxidation. Methods Enzymol. 1978; 52: 302-10. doi: 10.1016/s0076-6879(78)52032-6. [ DOI] [ PubMed] 16. Dziendzikowska K, Gromadzka-Ostrowska J, Lankoff A, Oczkowski M, Krawczyńska A, Chwastowska J, et al. Time-dependent biodistribution and excretion of silver nanoparticles in male Wistar rats. J Appl Toxicol. 2012 Nov; 32(11): 920-28. doi: 10.1002/jat.2758. [ DOI] [ PubMed] 17. Rahnama S, Heydarnejad MS, Mobini-Dehkordi M, Shadkhast M, Yarmohammadi-Samani P, Najafi M. A macro-and microscopic local effect of silver nanoparticles on skin wound healing and some biochemical parameters of blood in mice. J Shahrekord Univ Med Sci. 2014; 16(2): 80-89. [Article in Persian] [ View at Publisher] 18. Aydin B. Quercetin Prevents Methotrexate-induced Hepatotoxicity without Interfering Methotrexate Metabolizing Enzymes in Liver of Mice. Journal of Applied Biological Sciences. 2019; 5(2): 75-80. [ View at Publisher] 19. Wang Z, Xia T, Liu S. Mechanisms of nanosilver-induced toxicological effects: more attention should be paid to its sublethal effects. Nanoscale. 2015 May; 7(17): 7470-81. doi: 10.1039/c5nr01133g. [ DOI] [ PubMed] 20. Mazur P, Skiba-Kurek I, Mrowiec P, Karczewska E, Drożdż R. Synergistic ROS-Associated Antimicrobial Activity of Silver Nanoparticles and Gentamicin Against Staphylococcus epidermidis. Int J Nanomedicine. 2020 May; 15: 3551-62. doi: 10.2147/IJN.S246484. [ DOI] [ PubMed] 21. Edremitlioğlu M, Andıç MF, Korkut O. Quercetin, a powerful antioxidant bioflavonoid, prevents oxidative damage in different tissues of long-term diabetic rats. Balkan Med J. 2012; 29(1): 49-55. doi: 10.5152/balkanmedj.2011.002. [ View at Publisher] [ DOI] 22. Yousef MI, Omar SA, El-Guendi MI, Abdelmegid LA. Potential protective effects of quercetin and curcumin on paracetamol-induced histological changes, oxidative stress, impaired liver and kidney functions and haematotoxicity in rat. Food Chem Toxicol. 2010 Nov; 48(11): 3246-61. doi: 10.1016/j.fct.2010.08.034. [ DOI] [ PubMed] 23. Gopinath P, Gogoi SK, Chattopadhyay A, Ghosh SS. Implications of silver nanoparticle induced cell apoptosis for in vitro gene therapy. Nanotechnology. 2008 Feb; 19(7): 075104. doi: 10.1088/0957-4484/19/7/075104. [ DOI] [ PubMed] 24. Genter MB, Newman NC, Shertzer HG, Ali SF, Bolon B. Distribution and systemic effects of intranasally administered 25 nm silver nanoparticles in adult mice. Toxicol Pathol. 2012 Oct; 40(7): 1004-13. doi: 10.1177/0192623312444470. [ DOI] [ PubMed] 25. Roshan Rezaee Ranjbar S, Rezaei Zarchi S, Talebi A, Nasri S, Imani S, Khoradmehr A, et al. Toxicological effects of silver nanoparticles in rats. African Journal of Microbiology Research. 2012 Jul; 6(27): 5587-93. [ View at Publisher] 26. Heydarnejad MS, Rahnama S, Mobini-Dehkordi M, Yarmohammadi P, Aslnai H. Sliver nanoparticles accelerate skin wound healing in mice (Mus musculus) through suppression of innate immune system. Nanomedicine Journal. 2014; 1(2): 79-87. doi: 10.7508/nmj.2014.02.003. [ View at Publisher] [ DOI] |
|
| Send email to the article author |
|
|
|
|
