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:: Volume 24, Issue 3 (10-2022) ::
J Gorgan Univ Med Sci 2022, 24(3): 51-58 Back to browse issues page
Influence of Estrous Cycle Hormonal Fluctuations and Gonadal Hormones on Neurogenesis in the Subventricular Zone of Adult Mice
Mandana Emamdust1 , Mohammad Taghi Ghorbanian * 2, Fariba Banaian3
1- M.Sc in Developmental Biology, Department of Cellular and Molecular Biology, School of Biology, Damghan University, Damghan, Iran.
2- Associate Professor, Department of Cellular and Molecular Biology, School of Biology, Damghan University, Damghan, Iran. , ghorbanian@du.ac.ir
3- B.A in Clinical Psychology Semnan Education Organization, Damghan Education Management, Damghan, Iran.
Abstract:   (2555 Views)
Background and Objective: Neurogenesis is the process through which neurons are generated from neural stem cells. This process has been shown to occur in special zones of the adult brain including the subventricular zone (SVZ) of the lateral ventricles and the dentate gyrus of the hippocampus. Gonadal steroids affect different steps of neurogenesis, and cell proliferation seems to be increased by estrogens. This study aimed to investigate the neurogenic changes in the SVZ at different phases of the estrous cycle.
Methods: In this experimental study, 26 NMRI mice were used. The mice were identified by vaginal smear and then divided into 4 groups including proestrus (n=5), estrous (n=7), metestrus (n=7) and diestrous (n=7). Different stages of the estrous cycle were determined by staining vaginal smears. Also, the qualitative assessment of cell proliferation in the SVZ was performed by cresyl fast violet staining and glial fibrillary acidic protein (GFAP) immunohistochemistry at different stages of the estrous cycle.
Results: In microscopic sections stained with cresyl violet, it was observed that cell density in the proestrus stage of the estrous cycle was greater than in any other stages of the estrous cycle. A comparison of sections stained with anti-GFAP showed that the density of astrocytes in proestrus was significantly higher than in other groups.
Conclusion: Proestrus stage of the estrous cycle is associated with increased cell proliferation and density of astrocytes in the SVZ of mice. Neurogenesis is correlated to changes in sex hormonal levels at different phases of the estrus cycle.
 
Keywords: Subventricular Zone [MeSH], Neurogenesis [MeSH], Estrous Cycle [MeSH], Astrocyte [MeSH]
Article ID: Vol24-35
Full-Text [PDF 1285 kb]   (6933 Downloads)    
Type of Study: Original Articles | Subject: Stem Cells
References
1. Sager T, Kashon ML, Krajnak K. Estrogen and Environmental Enrichment Differentially Affect Neurogenesis, Dendritic Spine Immunolabeling and Synaptogenesis in the Hippocampus of Young and Reproductively Senescent Female Rats. Neuroendocrinology. 2018; 106(3): 252-63. DOI: 10.1159/000479699 [DOI] [PubMed]
2. Cable J, Fuchs E, Weissman I, Jasper H, Glass D, Rando TA, et al. Adult stem cells and regenerative medicine-a symposium report. Ann N Y Acad Sci. 2020 Feb; 1462(1): 27-36. DOI: 10.1111/nyas.14243 [DOI] [PubMed]
3. Rogers EH, Hunt JA, Pekovic-Vaughan V. Adult stem cell maintenance and tissue regeneration around the clock: do impaired stem cell clocks drive age-associated tissue degeneration? Biogerontology. 2018 Dec; 19(6): 497-517. DOI: 10.1007/s10522-018-9772-6 [DOI] [PubMed]
4. van Praag H, Schinder AF, Christie BR, Toni N, Palmer TD, Gage FH. Functional neurogenesis in the adult hippocampus. Nature. 2002 Feb; 415(6875): 1030-34. DOI: 10.1038/4151030a [DOI] [PubMed]
5. Kornack DR, Rakic P. The generation, migration, and differentiation of olfactory neurons in the adult primate brain. Proc Natl Acad Sci U S A. 2001 Apr; 98(8): 4752-57. DOI: 10.1073/pnas.081074998 [DOI] [PubMed]
6. Mami Matsumoto, Masato Sawada, Diego García-González, Vicente Herranz-Pérez, Takashi Ogino, Huy Bang Nguyen, et al. Dynamic Changes in Ultrastructure of the Primary Cilium in Migrating Neuroblasts in the Postnatal Brain. Journal of Neuroscience. 2019 Dec; 39(50): 9967-88. DOI: 10.1523/JNEUROSCI.1503-19.2019 [Link] [DOI]
7. Gubert F, Zaverucha-do-Valle C, Pimentel-Coelho PM, Mendez-Otero R, Santiago MF. Radial glia-like cells persist in the adult rat brain. Brain Res. 2009 Mar; 1258: 43-52. DOI: 10.1016/j.brainres.2008.12.021 [DOI] [PubMed]
8. Bond AM, Ming GL, Song H. Ontogeny of adult neural stem cells in the mammalian brain. Curr Top Dev Biol. 2021; 142: 67-98. DOI: 10.1016/bs.ctdb.2020.11.002 [DOI] [PubMed]
9. Arevalo MA, Ruiz-Palmero I, Scerbo MJ, Acaz-Fonseca E, Cambiasso MJ, Garcia-Segura LM. Molecular mechanisms involved in the regulation of neuritogenesis by estradiol: Recent advances. J Steroid Biochem Mol Biol. 2012 Aug; 131(1-2): 52-56. DOI: 10.1016/j.jsbmb.2011.09.004 [DOI] [PubMed]
10. Acaz-Fonseca E, Sanchez-Gonzalez R, Azcoitia I, Arevalo MA, Garcia-Segura LM. Role of astrocytes in the neuroprotective actions of 17β-estradiol and selective estrogen receptor modulators. Mol Cell Endocrinol. 2014 May; 389(1-2): 48-57. DOI: 10.1016/j.mce.2014.01.009 [DOI] [PubMed]
11. Matsuwaki T, Asakura R, Suzuki M, Yamanouchi K, Nishihara M. Age-dependent changes in progranulin expression in the mouse brain. J Reprod Dev. 2011 Feb; 57(1): 113-39. DOI: 10.1262/jrd.10-116s [DOI] [PubMed]
12. Sadrollahi M, Ghorbanian MT, Zavareh S. [Hippocampal neurogenesis in mice at different phases of the estrous cycle]. Feyz. 2014; 18(4): 336-44. [Article in Persian] [View at Publisher]
13. Ghorbanian M T, Ghorbanian M, Sadrollahi M. [Morphological changes of the hippocampal astrocytes at different phases of the estrous cycle in female mice]. Feyz. 2015; 19(2): 118-26. [Article in Persian] [View at Publisher]
14. Galea LA. Gonadal hormone modulation of neurogenesis in the dentate gyrus of adult male and female rodents. Brain Res Rev. 2008 Mar; 57(2): 332-41. DOI: 10.1016/j.brainresrev.2007.05.008 [DOI] [PubMed]
15. Arias C, Zepeda A, Hernández-Ortega K, Leal-Galicia P, Lojero C, Camacho-Arroyo I. Sex and estrous cycle-dependent differences in glial fibrillary acidic protein immunoreactivity in the adult rat hippocampus. Horm Behav. 2009 Jan; 55(1): 257-63. DOI: 10.1016/j.yhbeh.2008.10.016 [DOI] [PubMed]
16. Barker JM, Galea LA. Repeated estradiol administration alters different aspects of neurogenesis and cell death in the hippocampus of female, but not male, rats. Neuroscience. 2008 Apr; 152(4): 888-902. DOI: 10.1016/j.neuroscience.2007.10.071 [DOI] [PubMed]
17. Barha CK, Galea LA. Influence of different estrogens on neuroplasticity and cognition in the hippocampus. Biochim Biophys Acta. 2010 Oct; 1800(10): 1056-67. DOI: 10.1016/j.bbagen.2010.01.006 [DOI] [PubMed]
18. Westwood FR. The female rat reproductive cycle: a practical histological guide to staging. Toxicol Pathol. 2008 Apr; 36(3): 375-84. DOI: 10.1177/0192623308315665 [DOI] [PubMed]
19. Hubscher CH, Brooks DL, Johnson JR. A quantitative method for assessing stages of the rat estrous cycle. Biotech Histochem. 2005 Mar-Apr; 80(2): 79-87. DOI: 10.1080/10520290500138422 [DOI] [PubMed]
20. Falconer EM, Galea LA. Sex differences in cell proliferation, cell death and defensive behavior following acute predator odor stress in adult rats. Brain Res. 2003 Jun; 975(1-2): 22-36. DOI: 10.1016/s0006-8993(03)02542-3 [DOI] [PubMed]
21. Kight KE, McCarthy MM. Sex differences and estrogen regulation of BDNF gene expression, but not propeptide content, in the developing hippocampus. J Neurosci Res. 2017 Jan; 95(1-2): 345-54. DOI: 10.1002/jnr.23920 [DOI] [PubMed]
22. Mohr MA, DonCarlos LL, Sisk CL. Inhibiting Production of New Brain Cells during Puberty or Adulthood Blunts the Hormonally Induced Surge of Luteinizing Hormone in Female Rats. eNeuro. 2017 Nov; 4(5): ENEURO.0133-17.2017. DOI: 10.1523/ENEURO.0133-17.2017 [DOI] [PubMed]
23. Acaz-Fonseca E, Avila-Rodriguez M, Garcia-Segura LM, Barreto GE. Regulation of astroglia by gonadal steroid hormones under physiological and pathological conditions. Prog Neurobiol. 2016 Sep; 144: 5-26. DOI: 10.1016/j.pneurobio.2016.06.002 [DOI] [PubMed]
24. Pawluski JL, Brummelte S, Barha CK, Crozier TM, Galea LA. Effects of steroid hormones on neurogenesis in the hippocampus of the adult female rodent during the estrous cycle, pregnancy, lactation and aging. Front Neuroendocrinol. 2009 Aug; 30(3): 343-57. DOI: 10.1016/j.yfrne.2009.03.007 [DOI] [PubMed]
25. Heberden C. Sex steroids and neurogenesis. Biochem Pharmacol. 2017 Oct; 141: 56-62. DOI: 10.1016/j.bcp.2017.05.019 [DOI] [PubMed]
26. Yagi S, Drewczynski D, Wainwright SR, Barha CK, Hershorn O, Galea LAM. Sex and estrous cycle differences in immediate early gene activation in the hippocampus and the dorsal striatum after the cue competition task. Horm Behav. 2017 Jan; 87: 69-79. DOI: 10.1016/j.yhbeh.2016.10.019 [DOI] [PubMed]
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Emamdust M, Ghorbanian M T, Banaian F. Influence of Estrous Cycle Hormonal Fluctuations and Gonadal Hormones on Neurogenesis in the Subventricular Zone of Adult Mice. J Gorgan Univ Med Sci 2022; 24 (3) :51-58
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Volume 24, Issue 3 (10-2022) Back to browse issues page
مجله دانشگاه علوم پزشکی گرگان Journal of Gorgan University of Medical Sciences
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