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:: Volume 24, Issue 3 (10-2022) ::
J Gorgan Univ Med Sci 2022, 24(3): 51-58 Back to browse issues page
Influence of Estrous Cycle Hormonal Fluctuations and Gonadal Hormones on Neurogenesis in the Subventricular Zone of Adult Mice
Mandana Emamdust1 , Mohammad Taghi Ghorbanian * 2, Fariba Banaian3
1- M.Sc in Developmental Biology, Department of Cellular and Molecular Biology, School of Biology, Damghan University, Damghan, Iran.
2- Associate Professor, Department of Cellular and Molecular Biology, School of Biology, Damghan University, Damghan, Iran. , ghorbanian@du.ac.ir
3- B.A in Clinical Psychology Semnan Education Organization, Damghan Education Management, Damghan, Iran.
Abstract:   (3636 Views)
Background and Objective: Neurogenesis is the process through which neurons are generated from neural stem cells. This process has been shown to occur in special zones of the adult brain including the subventricular zone (SVZ) of the lateral ventricles and the dentate gyrus of the hippocampus. Gonadal steroids affect different steps of neurogenesis, and cell proliferation seems to be increased by estrogens. This study aimed to investigate the neurogenic changes in the SVZ at different phases of the estrous cycle.
Methods: In this experimental study, 26 NMRI mice were used. The mice were identified by vaginal smear and then divided into 4 groups including proestrus (n=5), estrous (n=7), metestrus (n=7) and diestrous (n=7). Different stages of the estrous cycle were determined by staining vaginal smears. Also, the qualitative assessment of cell proliferation in the SVZ was performed by cresyl fast violet staining and glial fibrillary acidic protein (GFAP) immunohistochemistry at different stages of the estrous cycle.
Results: In microscopic sections stained with cresyl violet, it was observed that cell density in the proestrus stage of the estrous cycle was greater than in any other stages of the estrous cycle. A comparison of sections stained with anti-GFAP showed that the density of astrocytes in proestrus was significantly higher than in other groups.
Conclusion: Proestrus stage of the estrous cycle is associated with increased cell proliferation and density of astrocytes in the SVZ of mice. Neurogenesis is correlated to changes in sex hormonal levels at different phases of the estrus cycle.
 
Keywords: Subventricular Zone [MeSH], Neurogenesis [MeSH], Estrous Cycle [MeSH], Astrocyte [MeSH]
Article ID: Vol24-35
Full-Text [PDF 1285 kb]   (8855 Downloads)    
Type of Study: Original Articles | Subject: Stem Cells
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Emamdust M, Ghorbanian M T, Banaian F. Influence of Estrous Cycle Hormonal Fluctuations and Gonadal Hormones on Neurogenesis in the Subventricular Zone of Adult Mice. J Gorgan Univ Med Sci 2022; 24 (3) :51-58
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Volume 24, Issue 3 (10-2022) Back to browse issues page
مجله دانشگاه علوم پزشکی گرگان Journal of Gorgan University of Medical Sciences
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