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Anticancer Activity of Copolymeric Nanoparticles Loaded by Plumbagin on Breast Cancer cells
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Hossein Danafar *    1, Ali Sharafi2 , Behrouz Parnianifar3 |
1- Associate Professor, Zanjan Pharmaceutical Biotechnology Research Center, Zanjan University of Medical Sciences, Zanjan, Iran. , danafar@zums.ac.ir
2- Assistant Professor, Zanjan Pharmaceutical Biotechnology Research Center, Zanjan University of Medical Sciences, Zanjan, Iran.
3- Doctor of Pharmacy, Zanjan Pharmaceutical Biotechnology Research Center, Zanjan University of Medical Sciences, Zanjan, Iran. |
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Abstract: (1661 Views) |
Background and Objective: In medical sciences, identifying the anticancer properties of plumbagin is of special importance. For this reason, this study investigated the anticancer activity of polymeric nanoparticles loaded by plumbagin against breast cancer cells.
Methods: In this descriptive study, the diblock copolymer mPEG–PCL was synthesized by ring-opening polymerization of caprolactone in the presence of mPEG as the initiator and Sn(oct)2 as the catalyst. The synthesized copolymers were characterized by Fourier-transform infrared spectroscopy, proton nuclear magnetic resonance, gel permeation chromatography, and differential scanning calorimetry. The nanoprecipitation method was used for preparing nanoparticles loaded with plumbagin. The characteristics of these nanoparticles were investigated by various techniques including dynamic light scattering. The cytotoxicity of plumbagin, copolymer, and the nanoparticles loaded with plumbagin on MCF7 and HFF2 cells was evaluated by MTT assay.
Results: The average diameter of the nanoparticles was less than 115 nm. The loading capacity and encapsulation efficiencies were 15.4±0.13% and 79.1±0.65%, respectively. Drug release was slow, controlled, and almost dependent on pH. The results of the MTT assay showed strong and dose-dependent inhibition of cell growth by the plumbagin-loaded micelles compared with plumbagin alone in a way that the half maximal inhibitory concentration of this nanoparticle against MCF7 cells after 48 and 72 hours was 10.78 and 24.03 μM, respectively.
Conclusion: The mPEG-PCL nanoparticles can be an efficient carrier for plumbagin, and plumbagin can be an effective drug on breast cancer cells, without toxicity on healthy cells.
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Keywords: Plumbagin [MeSH], Cytotoxicity , Nanoparticles [MeSH], diBlock copolymers
Article ID: Vol24-26 |
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Full-Text [PDF 752 kb]
(8941 Downloads)
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Type of Study: Original Articles |
Subject:
Nanobiotecnology
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1. Bhattacharya A, Jindal B, Singh P, Datta A, Panda D. Plumbagin inhibits cytokinesis in Bacillus subtilis by inhibiting FtsZ assembly--a mechanistic study of its antibacterial activity. FEBS J. 2013 Sep; 280(18): 4585-99. DOI: 10.1111/febs.12429 [ DOI] [ PubMed] 2. Jamal MS, Parveen S, Beg MA, Suhail M, Chaudhary AG, Damanhouri GA, et al. Anticancer compound plumbagin and its molecular targets: a structural insight into the inhibitory mechanisms using computational approaches. PLoS One. 2014 Feb; 9(2): e87309. DOI: 10.1371/journal.pone.0087309 [ DOI] [ PubMed] 3. Hafeez BB, Zhong W, Fischer JW, Mustafa A, Shi X, Meske L, Hong H, et al. Plumbagin, a medicinal plant (Plumbago zeylanica)-derived 1,4-naphthoquinone, inhibits growth and metastasis of human prostate cancer PC-3M-luciferase cells in an orthotopic xenograft mouse model. Mol Oncol. 2013 Jun; 7(3): 428-39. DOI: 10.1016/j.molonc.2012.12.001 [ DOI] [ PubMed] 4. Chen CA, Chang HH, Kao CY, Tsai TH, Chen YJ. Plumbagin, isolated from Plumbago zeylanica, induces cell death through apoptosis in human pancreatic cancer cells. Pancreatology. 2009; 9(6): 797-809. DOI: 10.1159/000210028 [ DOI] [ PubMed] 5. Demma J, Hallberg K, Hellman B. Genotoxicity of plumbagin and its effects on catechol and NQNO-induced DNA damage in mouse lymphoma cells. Toxicol In Vitro. 2009 Mar; 23(2): 266-71. DOI: 10.1016/j.tiv.2008.12.007 [ DOI] [ PubMed] 6. Jackson JK, Higo T, Hunter WL, Burt HM. Topoisomerase inhibitors as anti-arthritic agents. Inflamm Res. 2008 Mar; 57(3): 126-34. DOI: 10.1007/s00011-007-7163-6 [ DOI] [ PubMed] 7. Srinivas P, Gopinath G, Banerji A, Dinakar A, Srinivas G. Plumbagin induces reactive oxygen species, which mediate apoptosis in human cervical cancer cells. Mol Carcinog. 2004 Aug; 40(4): 201-11. DOI: 10.1002/mc.20031 [ DOI] [ PubMed] 8. Thasni KA, Rakesh S, Rojini G, Ratheeshkumar T, Srinivas G, Priya S. Estrogen-dependent cell signaling and apoptosis in BRCA1-blocked BG1 ovarian cancer cells in response to plumbagin and other chemotherapeutic agents. Ann Oncol. 2008 Apr; 19(4): 696-705. DOI: 10.1093/annonc/mdm557 [ DOI] [ PubMed] 9. Reis CP, Neufeld RJ, Ribeiro AJ, Veiga F. Nanoencapsulation I. Methods for preparation of drug-loaded polymeric nanoparticles. Nanomedicine. 2006 Mar; 2(1): 8-21. DOI: 10.1016/j.nano.2005.12.003 [ DOI] [ PubMed] 10. Thakkar KN, Mhatre SS, Parikh RY. Biological synthesis of metallic nanoparticles. Nanomedicine: Nanotechnology, Biology and Medicine. 2010; 6(2): 257-62. DOI: 10.1016/j.nano.2009.07.002 [ Article] [ DOI] 11. Wei X, Gong C, Gou M, Fu S, Guo Q, Shi S, et al. Biodegradable poly(epsilon-caprolactone)-poly(ethylene glycol) copolymers as drug delivery system. Int J Pharm. 2009 Oct; 381(1): 1-18. DOI: 10.1016/j.ijpharm.2009.07.033 [ DOI] [ PubMed] 12. Shi HS, Gao X, Li D, Zhang QW, Wang YS, Zheng Y, et al. A systemic administration of liposomal curcumin inhibits radiation pneumonitis and sensitizes lung carcinoma to radiation. Int J Nanomedicine. 2012; 7: 2601-11. DOI: 10.2147/IJN.S31439 [ DOI] [ PubMed] 13. Men K, Gou ML, Guo QF, Wang XH, Shi S, Kan B, et al. A novel drug and gene co-delivery system based on Poly(epsilon-caprolactone)-Poly(ethylene glycol)-Poly(epsilon-caprolactone) grafted polyethyleneimine micelle. J Nanosci Nanotechnol. 2010 Dec; 10(12): 7958-64. DOI: 10.1166/jnn.2010.2668 [ DOI] [ PubMed] 14. Gou M, Wei X, Men K, Wang B, Luo F, Zhao X, et al. PCL/PEG copolymeric nanoparticles: potential nanoplatforms for anticancer agent delivery. Curr Drug Targets. 2011 Jul; 12(8): 1131-50. DOI: 10.2174/138945011795906642 [ DOI] [ PubMed] 15. Appadurai P, Rathinasamy K. Plumbagin-silver nanoparticle formulations enhance the cellular uptake of plumbagin and its antiproliferative activities. IET Nanobiotechnol. 2015 Oct; 9(5): 264-72. DOI: 10.1049/iet-nbt.2015.0008 [ DOI] [ PubMed] 16. Pan M, Li W, Yang J, Li Z, Zhao J, Xiao Y, et al. Plumbagin-loaded aptamer-targeted poly D,L-lactic-co-glycolic acid-b-polyethylene glycol nanoparticles for prostate cancer therapy. Medicine (Baltimore). 2017 Jul; 96(30): e7405. DOI: 10.1097/MD.0000000000007405 [ DOI] [ PubMed] 17. Chrastina A, Baron VT, Abedinpour P, Rondeau G, Welsh J, Borgström P. Plumbagin-Loaded Nanoemulsion Drug Delivery Formulation and Evaluation of Antiproliferative Effect on Prostate Cancer Cells. Biomed Res Int. 2018 Nov; 2018: 9035452. DOI: 10.1155/2018/9035452 [ DOI] [ PubMed] |
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