[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Journal Information::
Indexing Sources::
Editorial Board::
Executive Members::
Articles Archive::
Instruction to Authors::
Contact Us::
Site Facilities::
Search in website

Advanced Search
Receive site information
Enter your Email in the following box to receive the site news and information.
:: Volume 24, Issue 1 (3-2022) ::
J Gorgan Univ Med Sci 2022, 24(1): 44-52 Back to browse issues page
The Simultaneous Effect of Resistance Training and Endothelial Progenitor Cells Injection on Blood Glucose Level, TNF-α and IL-10 Protein Expression of Muscular Cells of Diabetic Male Rats
Soren Valafar1 , Eidy Alijani * 2, Fariba Aghaei3 , Mahsa Mohsenzadeh3
1- Ph.D Candidate in Sports Physiology, College of Physical Education and Sport Sciences, Karaj Branch, Islamic Azad University, Karaj, Iran.
2- Professor of Sports Physiology, College of Physical Education and Sport Sciences, Karaj Branch, Islamic Azad University, Karaj, Iran. , eidyalijani@yahoo.com
3- Assistant Professor of Sports Physiology, College of Physical Education and Sport Sciences, Karaj Branch, Islamic Azad University, Karaj, Iran.
Abstract:   (3278 Views)
Background and Objective: Type 1 diabetes (T1D) is highly prevalent in the group of autoimmune and inflammatory patients. Tumor necrosis factor-alpha (TNF-a) and interleukin-10 (IL-10) plays an important role in regulating complex interactions between pancreatic beta cells and immune cells in the development of T1D. This study was performed to determine the simultaneous effect of resistance training and endothelial progenitor cell injection on blood glucose levels and protein expression of proinflammatory factors TNF-a and IL-10 in muscle tissue of streptozotocin (STZ) induced diabetic male rats.
Methods: In this experimental study, 36 male Wistar rats weighing approximately 200±20 g and six weeks old were randomly divided into six groups. Induction of diabetes was performed by intraperitoneal injection of STZ at a dose of 45 mg/kg body weight. Groups included diabetes + stem cell injection + resistance training group, diabetes + resistance training group, diabetes + stem cell injection group, control diabetic group to control the passage of time, and healthy basal and diabetic groups for defaults. Exercises were performed for 17 sessions of resistance training, including climbing ladders with increasing weight three days a week in the same laboratory conditions. Endothelial progenitor cells were cultured by femoral bone marrow aspiration and culture and then injection into the tail vein. 68 hours after the last training session, blood glucose levels were assessed by ELISA and the expression of TNF-a and IL-10 protein in muscle tissue was assessed by Western blotting.
Results: Endothelial stem cell injection, resistance training and resistance training with the simultaneous injection of endothelial stem cells significantly increased the anti-inflammatory factor IL-10 in the skeletal muscle tissue of diabetic rats in compared to control group (P<0.05). Expression of the anti-inflammatory factor IL-10 in the skeletal muscle tissue was significantly increased in resistance training plus the simultaneous injection of endothelial stem cells group in compared to injection of stem cells and resistance training groups (P<0.05). Glucose concentration in the skeletal muscle tissue was significantly reduced in resistance training plus the simultaneous injection of endothelial stem cells group in compared to injection of stem cells and resistance training groups (P<0.05).
Conclusion: It seems that 17 sessions of resistance training reduces blood glucose level and improves inflammatory conditions in response to an increase in IL-10 and a decrease in TNF-a in a group of diabetic rats with resistance training and simultaneous injection of endothelial progenitor cells in diabetic male rats.
Keywords: Resistance Training [MeSH], Endothelial Progenitor Cells [MeSH], Type 1 Diabetes Mellitus [MeSH], Blood Glucose [MeSH], Tumor Necrosis Factor-alpha [MeSH], Interleukin-10 [MeSH]
Article ID: Vol24-06
Full-Text [PDF 849 kb]   (8981 Downloads)    
Type of Study: Experimental Study | Subject: Immunology
1. Yiu KH, Tse HF. Specific role of impaired glucose metabolism and diabetes mellitus in endothelial progenitor cell characteristics and function. Arterioscler Thromb Vasc Biol. 2014 Jun; 34(6): 1136-43. DOI: 10.1161/ATVBAHA.114.302192 [DOI] [PubMed]
2. Kim JS, Lee YH, Kim JC, Ko YH, Yoon CS, Yi HK. Effect of exercise training of different intensities on anti-inflammatory reaction in streptozotocin-induced diabetic rats. Biol Sport. 2014 Mar; 31(1): 73-79. DOI: 10.5604/20831862.1093775 [DOI] [PubMed]
3. Naushad N, Perdigoto AL, Rui J, Herold KC. Have we pushed the needle for treatment of Type 1 diabetes? Curr Opin Immunol. 2017 Dec; 49: 44-50. DOI: 10.1016/j.coi.2017.09.004 [DOI] [PubMed]
4. DiMeglio LA, Evans-Molina C, Oram RA. Type 1 diabetes. Lancet. 2018 Jun; 391(10138): 2449-62. DOI: 10.1016/S0140-6736(18)31320-5 [DOI] [PubMed]
5. Abbas AK, Lichtman AH, Pillai S. [Basic immunology: functions and disorders of the immune system]. Translation by: Kazemi T, Abbasi H, Seifizadeh N, Seifizadeh N, Bagheri S. 5th ed. Tehran: Ebnesina Publication. 2016. [Persian]
6. Zamanpour L, Banitalebi E, Amirhosseini SE. [The Effect Of Sprint Training And Combined Aerobic And Strength Training On Some Inflammatory Markers And Insulin Resistance In Women With Diabetes Mellitus (T2dm)]. Diabetes Metabol. 2016; 15(5): 300-11. [Article in Persian] [View at Publisher]
7. Lu J, Liu J, Li L, Lan Y, Liang Y. Cytokines in type 1 diabetes: mechanisms of action and immunotherapeutic targets. Clin Transl Immunology. 2020 Mar; 9(3): e1122. DOI: 10.1002/cti2.1122 [DOI] [PubMed]
8. Ko KI, Coimbra LS, Tian C, Alblowi J, Kayal RA, Einhorn TA, et al. Diabetes reduces mesenchymal stem cells in fracture healing through a TNFα-mediated mechanism. Diabetologia. 2015 Mar; 58(3): 633-42. DOI: 10.1007/s00125-014-3470-y [DOI] [PubMed]
9. Li C, Zhang L, Chen Y, Lin X, Li T. Protective role of adenovirus vector-mediated interleukin-10 gene therapy on endogenous islet β-cells in recent-onset type 1 diabetes in NOD mice. Exp Ther Med. 2016 May; 11(5): 1625-32. DOI: 10.3892/etm.2016.3169 [DOI] [PubMed]
10. Robert S, Gysemans C, Takiishi T, Korf H, Spagnuolo I, Sebastiani G, et al. Oral delivery of glutamic acid decarboxylase (GAD)-65 and IL10 by Lactococcus lactis reverses diabetes in recent-onset NOD mice. Diabetes. 2014 Aug; 63(8): 2876-87. DOI: 10.2337/db13-1236 [DOI] [PubMed]
11. Farazandeh Nia D, Hosseini M, Riyahi Malayeri S, Daneshjoo A. [Effect of Eight Weeks of Swimming Training with Garlic Intake on Serum Levels of IL-10 and TNF-α in Obese Male Rats]. Jundishapur Sci Med J. 2018; 16(6): 665-71. DOI: 10.22118/jsmj.2018.57830 [Article in Persian] [View at Publisher] [DOI]
12. Makki K, Froguel P, Wolowczuk I. Adipose tissue in obesity-related inflammation and insulin resistance: cells, cytokines, and chemokines. ISRN Inflamm. 2013 Dec; 2013: 139239. DOI: 10.1155/2013/139239 [DOI] [PubMed]
13. Nouri Barkestani M, Shamdani S, Afshar Bakshloo M, Arouche N, Bambai B, Uzan G, et al. TNFα priming through its interaction with TNFR2 enhances endothelial progenitor cell immunosuppressive effect: new hope for their widespread clinical application. Cell Commun Signal. 2021 Jan; 19(1): 1. DOI: 10.1186/s12964-020-00683-x [DOI] [PubMed]
14. Rezaei S, Matinhomaee H, Azarbayjani MA, Farzanegi P. [Effect of Interval Training Intensity on Gene Expression of Endothelial Progenitor Cells and Cardiac Stem Cells in Aged Rats]. J Ilam Univ Med Sci. 2018; 26(3): 27-37. DOI: 10.29252/sjimu.26.3.27 [Article in Persian] [View at Publisher] [DOI]
15. Nadri S, Saburi E, Nadri P, Brati G. [Isolation Methods of Mesenchymal Stem Cells in Mice; a Systematic Review]. J Advanced Biomed Sci. 2017; 6 (4): 417-25. [View at Publisher]
16. Wang Y, Xu C, Liang Y, Vanhoutte PM. SIRT1 in metabolic syndrome: where to target matters. Pharmacol Ther. 2012 Dec; 136(3): 305-18. DOI: 10.1016/j.pharmthera.2012.08.009 [DOI] [PubMed]
17. Asahara T, Murohara T, Sullivan A, Silver M, van der Zee R, Li T, et al. Isolation of putative progenitor endothelial cells for angiogenesis. Science. 1997 Feb; 275(5302): 964-67. DOI: 10.1126/science.275.5302.964 [DOI] [PubMed]
18. Gong X, Li B, Yang Y, Huang Y, Sun Y, Liu M, et al. Bone marrow derived endothelial progenitor cells retain their phenotype and functions after a limited number of culture passages and cryopreservation. Cytotechnology. 2019 Feb; 71(1): 1-14. DOI: 10.1007/s10616-018-0234-4 [DOI] [PubMed]
19. Chen C, Zheng S, Zhang X, Dai P, Gao Y, Nan L, et al. Transplantation of Amniotic Scaffold-Seeded Mesenchymal Stem Cells and/or Endothelial Progenitor Cells From Bone Marrow to Efficiently Repair 3-cm Circumferential Urethral Defect in Model Dogs. Tissue Eng Part A. 2018 Jan; 24(1-2): 47-56. DOI: 10.1089/ten.TEA.2016.0518 [DOI] [PubMed]
20. Dignat-George F, Sampol J. Circulating endothelial cells in vascular disorders: new insights into an old concept. Eur J Haematol. 2000 Oct; 65(4): 215-20. DOI: 10.1034/j.1600-0609.2000.065004215.x [DOI] [PubMed]
21. Proschinger S, Joisten N, Rademacher A, Schlagheck ML, Walzik D, Metcalfe AJ, et al. Influence of combined functional resistance and endurance exercise over 12 weeks on matrix metalloproteinase-2 serum concentration in persons with relapsing-remitting multiple sclerosis - a community-based randomized controlled trial. BMC Neurol. 2019 Dec; 19(1): 314. DOI: 10.1186/s12883-019-1544-7 [DOI] [PubMed]
22. Shih KC, Kwok CF. Exercise reduces body fat and improves insulin sensitivity and pancreatic β-cell function in overweight and obese male Taiwanese adolescents. BMC Pediatr. 2018 Feb; 18(1): 80. DOI: 10.1186/s12887-018-1025-y [DOI] [PubMed]
23. Colberg SR, Sigal RJ, Fernhall B, Regensteiner JG, Blissmer BJ, Rubin RR, et al. Exercise and type 2 diabetes: the American College of Sports Medicine and the American Diabetes Association: joint position statement. Diabetes Care. 2010 Dec; 33(12): e147-67. DOI: 10.2337/dc10-9990 [DOI] [PubMed]
24. Inoue DS, De Mello MT, Foschini D, Lira FS, De Piano Ganen A, Da Silveira Campos RM, et al. Linear and undulating periodized strength plus aerobic training promote similar benefits and lead to improvement of insulin resistance on obese adolescents. J Diabetes Complications. 2015 Mar; 29(2): 258-64. DOI: 10.1016/j.jdiacomp.2014.11.002 [DOI] [PubMed]
25. West DW, Lee-Barthel A, McIntyre T, Shamim B, Lee CA, Baar K. The exercise-induced biochemical milieu enhances collagen content and tensile strength of engineered ligaments. J Physiol. 2015 Oct; 593(20): 4665-75. DOI: 10.1113/JP270737 [DOI] [PubMed]
26. Mohamed MT, Embaby EA, Labib A, El-Husseiny M, Khamis H, El-Demery A, et al. Effects of exercise in combination with autologous bone marrow stem cell transplantation for patients with type 1 diabetes. Physiother Theory Pract. 2019 Dec; 35(12): 1233-42. DOI: 10.1080/09593985.2018.1474511 [DOI] [PubMed]
27. Rezaee S, Farzanegi P, Azarbaijani MA. [Role of Endothelial progenitor cells in angiogenesis with the approach of physical activity]. J Neyshabur Univ Med Sci. 2020; 8(2): 1-17. [Article in Persian] [View at Publisher]
28. Molanouri Shamsi M, Hassan ZM, Mahdavi M, Gharakhanlou R, Azadmanesh K, Baghersad L, et al. [Influence of Resistance Training on IL-15 mRNA Expression and the Protein Content in Slow and Fast Twitch Muscles of Diabetic Rats]. Iranian Endocrin Metabol. 2012; 14(2): 185-92. [Article in Persian] [View at Publisher]
29. Shekarchizade P. [The effect of resistance training on the capillary density of skeletal muscle and plasma levels of several factors associated with normal and diabetic rats]. Ph.D Dissertation. [Iran. Faculty of Humanities. Tarbiat Modarres University. 2013. [Persian]
30. Ozkol H, Tuluce Y, Dilsiz N, Koyuncu I. Therapeutic potential of some plant extracts used in Turkish traditional medicine on streptozocin-induced type 1 diabetes mellitus in rats. J Membr Biol. 2013 Jan; 246(1): 47-55. DOI: 10.1007/s00232-012-9503-x [DOI] [PubMed]
31. Somboonwong J, Traisaeng S, Saguanrungsirikul S. Moderate-intensity exercise training elevates serum and pancreatic zinc levels and pancreatic ZnT8 expression in streptozotocin-induced diabetic rats. Life Sci. 2015 Oct; 139: 46-51. DOI: 10.1016/j.lfs.2015.08.008 [DOI] [PubMed]
32. Khosravi N, Ravasi AA, Sharifi F. [Effect of two different intensity of physical activity on circulating endothelial progenitor cells (EPC) in healthy young women]. Research in Sport Medicine and Technology. 2011; 9(2): 67-78. [Article in Persian] [View at Publisher]
33. Soltanian Z, Vanaky B, Ramezanifard N, Shakeri N, Shams Z, Fakhari Rad F. [Effect of Eight Weeks Resistance Training On Gene Expression of TNF-Α and IL10 in the Heart of Type II Diabetic Male Rats]. J Shahid Sadoughi Uni Med Sci. 2019; 27(6): 1656-67. DOI: 10.18502/ssu.v27i6.1600 [Article in Persian] [View at Publisher] [DOI]
34. Miller EG, Sethi P, Nowson CA, Dunstan DW, Daly RM. Effects of progressive resistance training and weight loss versus weight loss alone on inflammatory and endothelial biomarkers in older adults with type 2 diabetes. Eur J Appl Physiol. 2017 Aug; 117(8): 1669-78. DOI: 10.1007/s00421-017-3657-2 [DOI] [PubMed]
35. Chen X, Sun X, Wang C, He H. Effects of Exercise on Inflammatory Cytokines in Patients with Type 2 Diabetes: A Meta-analysis of Randomized Controlled Trials. Oxid Med Cell Longev. 2020 Dec; 2020: 6660557. DOI: 10.1155/2020/6660557 [DOI] [PubMed]
36. Schaan BD, Waclawovsky G, Umpierre D, Figueira FR, de Lima ES, Alegretti AP, et al. A single session of aerobic or resistance exercise modifies the endothelial progenitor cell levels in healthy subjects, but not in individuals with type 1 diabetes. Diabetol Metab Syndr. 2015 Nov; 7(Suppl 1): A251. DOI: 10.1186/1758-5996-7-S1-A251 [DOI] [PubMed]
37. Mohammadnezhad B, Hashemvarzi SA, Farzane Hessari A. [The effect of resistance training with mesenchymal stem cell injection on TNF-α and kappa B nuclear factor levels in the cortex of streptozotocin-induced diabetic rats]. J Jiroft Univ Med Sci. 2020; 7(3): 414-21. [Article in Persian] [View at Publisher]
38. Dai X, Zhai L, Su Q, Luo B, Wei C, Liu Y, et al. Effect of Aerobic and Resistance Training on Endothelial Progenitor Cells in Mice with Type 2 Diabetes. Cell Reprogram. 2020 Aug; 22(4): 189-97. DOI: 10.1089/cell.2019.0063 [DOI] [PubMed]
Send email to the article author

XML   Persian Abstract   Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Valafar S, Alijani E, Aghaei F, Mohsenzadeh M. The Simultaneous Effect of Resistance Training and Endothelial Progenitor Cells Injection on Blood Glucose Level, TNF-α and IL-10 Protein Expression of Muscular Cells of Diabetic Male Rats. J Gorgan Univ Med Sci 2022; 24 (1) :44-52
URL: http://goums.ac.ir/journal/article-1-3983-en.html

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 24, Issue 1 (3-2022) Back to browse issues page
مجله دانشگاه علوم پزشکی گرگان Journal of Gorgan University of Medical Sciences
Persian site map - English site map - Created in 0.05 seconds with 36 queries by YEKTAWEB 4657