[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Home::
Journal Information::
About the Journal
Aims and Scope
Content Coverage
Conflict of Interest
Publication Ethics
Editorial Board::
Executive Members::
Instruction to Authors::
Peer Review::
Articles Archive::
Current Issue
All Issues
Indexing Databases::
Contact Us::
Contact Us
Contact Form
Site Facilities::
Search contents
::
Search in website

Advanced Search
Receive site information
Enter your Email in the following box to receive the site news and information.
:: Volume 23, Issue 1 (3-2021) ::
J Gorgan Univ Med Sci 2021, 23(1): 55-63 Back to browse issues page
A survey of moderate interval training on expression of mir-126, mir-1185 AKT and VCAM in cardiac tissue of rats with allergic asthma
Masoumeh Nobahar1 , Marefat Siahkouhian * 2, Shadmehr Mirdar3
1- Ph.D Candidate in Exercise Physiology, Department of Exercise Physiology, Faculty of Education and Psychology, University of Mohaghegh Ardabili, Ardabil, Iran.
2- Professor of Sport Physiology, Department of Sport Physiology, Faculty of Educational Sciences and Psychology, University of Mohaghegh Ardabili, Ardabil, Iran. , m_siahkohian@uma.ac.ir
3- Professor of Exercise Physiology, Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Mazandaran, Babolsar, Iran. Athletic Performance and Health Research Center, University of Mazandaran, Babolsar, Iran.
Abstract:   (7685 Views)
Background and Objective: People with asthma are at increased risk for cardiovascular disease and increased risk of death. Asthma is associated with the structure and function of vessel changes. In this regard, MicroRNAs, AKT and VCAM play an important role in endothelial function. So, the aim of this study was to investigate the effect of 12 weeks of interval training on expression of mir-126, mir-1185 AKT and VCAM in cardiac tissue rats with allergic asthma.
Methods: This experimental study was performed on 15 Wistar rats. Animals were randomly allocated into three groups (n=5) including healthy control, asthma control and asthmatic interval training. Ovalbumin solution was injected subcutaneously 3 times per week to asthma groups, and suffering from asthma was confirmed by increasing the amount of IgE. The asthma group participated in 8-week exercise training. The rats were anesthetized at the end of 8 weeks and then their heart tissue was isolated by surgical instrument and immediately placed in nitrogen fluid. Expression of mir-126, mir-1185, AKT and VCAM in rats cardiac tissue were evaluated.
Results: AKT and VCAM expression was significantly increased in asthma control group compared to healthy control group (P<0.05). mir-126, AKT and VCAM expression were significantly increased in asthmatic interval training group in compared to asthma control group (P<0.05) while Mir-1185 was significantly reduced in asthmatic interval training group in compared to asthma control group (P<0.05).
Conclusion: Interval training by increasing mir-126, by reducing the expression of mir-1185, AKT, and VCAM reduces the risk of atherosclerosis in rats affected with asthma.
Keywords: Asthma [MeSH], Interval Training [MeSH], mir-126 [MeSH], mir-1185 [MeSH], AKT [MeSH], VCAM [MeSH]
Article ID: Vol23-07
Full-Text [PDF 823 kb]   (14487 Downloads)    
Type of Study: Original Articles | Subject: Asthma and Allergy
References
1. Ober C,Yao TC. The genetics of asthma and allergic disease: a 21st century perspective. Immunol Rev. 2011 Jul; 242(1): 10-30. DOI: 10.1111/j.1600-065X.2011.01029.x [DOI] [PubMed]
2. Tuleta I, Skowasch D, Aurich F, Eckstein N, Schueler R, Pizarro C, et al. Asthma is associated with atherosclerotic artery changes. PLoS One. 2017; 12(10): e0186820. DOI: 10.1371/journal.pone.0186820 [DOI] [PubMed]
3. Wang L, Gao S, Yu M, Sheng Z, Tan W. Association of asthma with coronary heart disease: A meta analysis of 11 trials. PLoS One. 2017; 12(6): e0179335. DOI: 10.1371/journal.pone.0179335 [DOI] [PubMed]
4. Yun HD, Knoebel E, Fenta Y, Gabriel SE, Leibson CL, Loftus Jr EV, et al. Asthma and Proinflammatory Conditions: A Population-Based Retrospective Matched Cohort Study. Mayo Clin Proc. 2012 Oct; 87(10): 953-60. DOI: 10.1016/j.mayocp.2012.05.020 [DOI] [PubMed]
5. Knoflach M, Kiechl S, Mayr A, Willeit J, Poewe W, Wick G. Allergic rhinitis, asthma, and atherosclerosis in the Bruneck and ARMY studies. Arch Intern Med. 2005 Nov; 165(21): 2521-26. DOI: 10.1001/archinte.165.21.2521 [DOI] [PubMed]
6. Yoo EJ, Ojiaku CA, Sunder K, Panettieri Jr RA. Phosphoinositide 3-Kinase in Asthma: Novel Roles and Therapeutic Approaches. Am J Respir Cell Mol Biol. 2017 Jun; 56(6): 700-707. DOI: 10.1165/rcmb.2016-0308TR [DOI] [PubMed]
7. Black JL, Panettieri RA, Banerjee A, Berger P. Airway smooth muscle in asthma: just a target for bronchodilation? Clin Chest Med. 2012 Sep; 33(3):5 43-58. DOI: 10.1016/j.ccm.2012.05.002 [DOI] [PubMed]
8. Lambrecht BN, Hammad H. The airway epithelium in asthma. Nat Med. 2012 May; 18(5): 684-92. DOI: 10.1038/nm.2737 [DOI] [PubMed]
9. Zhai C, Cheng J, Mujahid H, Wang H, Kong J, Yin Y, et al. Selective inhibition of PI3K/Akt/mTOR signaling pathway regulates autophagy of macrophage and vulnerability of atherosclerotic plaque. PLoS One. 2014 Mar; 9(3): e90563. DOI: 10.1371/journal.pone.0090563 [DOI] [PubMed]
10. Rui W, Guan L, Zhang F, Zhang W, Ding W. PM2.5-induced oxidative stress increases adhesion molecules expression in human endothelial cells through the ERK/AKT/NF-κB-dependent pathway. J Appl Toxicol. 2016 Jan; 36(1): 48-59. DOI: 10.1002/jat.3143 [DOI] [PubMed]
11. Boulet L-P, FitzGerald JM, Reddel HK. The revised 2014 GINA strategy report: opportunities for change. Curr Opin Pulm Med. 2015 Jan; 21(1): 1-7. DOI: 10.1097/MCP.0000000000000125 [DOI] [PubMed]
12. Goto C, Higashi Y, Kimura M, Noma K, Hara K, Nakagawa K, et al. Effect of different intensities of exercise on endothelium-dependent vasodilation in humans: role of endothelium-dependent nitric oxide and oxidative stress. Circulation. 2003 Aug; 108(5): 530-35. DOI: 10.1161/01.CIR.0000080893.55729.28 [DOI] [PubMed]
13. Hamzaoui A, Ammar J, El Mekki F, Borgi O, Ghrairi H, Ben Brahim M, et al. Elevation of serum soluble E-selectin and VCAM-1 in severe asthma. Mediators Inflamm. 2001 Dec; 10(6): 339-42. DOI: 10.1080/09629350120102361 [DOI] [PubMed]
14. Latronico MVG, Condorelli G. MicroRNAs and cardiac pathology. Nat Rev Cardiol. 2009 Jun; 6(6): 419-29. DOI: 10.1038/nrcardio.2009.56 [DOI] [PubMed]
15. Qu M, Pan J, Shi X, Zhang Z, Tang Y, Yang G. MicroRNA-126 is a prospective target for vascular disease. Neuroimmunol Neuroinflammation. 2018; 5:10. DOI: 10.20517/2347-8659.2018.01 [DOI] [PubMed]
16. Wang S, Aurora AB, Johnson BA, Qi X, McAnally J, Hill JA, et al. The endothelial-specific microRNA miR-126 governs vascular integrity and angiogenesis. Dev Cell. 2008 Aug; 15(2): 261-71. DOI: 10.1016/j.devcel.2008.07.002 [DOI] [PubMed]
17. Wu XB, Wang MY, Zhu HY, Tang SQ, You YD, Xie YQ. Overexpression of microRNA-21 and microRNA-126 in the patients of bronchial asthma. Int J Clin Exp Med. 2014 May; 7(5): 1307-12. [PubMed]
18. Tian M, Ji Y, Wang T, Zhang W, Zhou Y, Cui Y. Changes in circulating microRNA-126 levels are associated with immune imbalance in children with acute asthma. Int J Immunopathol Pharmacol. 2018 Jan-Dec; 32: 2058738418779243. DOI: 10.1177/2058738418779243 [DOI] [PubMed]
19. Deng H. Chu X. Song Z. Deng X. Xu H. Ye Y. MicroRNA-1185 Induces Endothelial Cell Apoptosis by Targeting UVRAG and KRIT1. Cell Physiol Biochem. 2017; 41: 2171-82. DOI: 10.1159/000475571 [View at Publisher] [DOI]
20. Deng H, Song Z, Xu H, Deng X, Zhang Q, Chen H, et al. MicroRNA-1185 Promotes Arterial Stiffness though Modulating VCAM-1 and E-Selectin Expression. Cell Physiol Biochem. 2017; 41(6): 2183-93. DOI: 10.1159/000475576 [DOI] [PubMed]
21. Nanoudis S, Pikilidou M, Yavropoulou M, Zebekakis P. The Role of MicroRNAs in Arterial Stiffness and Arterial Calcification. An Update and Review of the Literature. Front Genet. 2017; 8: 209. DOI: 10.3389/fgene.2017.00209 [DOI] [PubMed]
22. Kadoglou NPE, Iliadis F, Liapis CD. Exercise and carotid atherosclerosis. Eur J Vasc Endovasc Surg. 2008 Mar; 35(3): 264-72. DOI: 10.1016/j.ejvs.2007.08.022 [DOI] [PubMed]
23. Silva RA, Almeida FM, Olivo CR, Saraiva Romanholo BM, Martins MA, Carvalho CRF. Exercise reverses OVA-induced inhibition of glucocorticoid receptor and increases anti-inflammatory cytokines in asthma. Scand J Med Sci Sports. 2016 Jan; 26(1): 82-92. DOI: 10.1111/sms.12411 [DOI] [PubMed]
24. Agostini D, Natalucci V, Baldelli G, De Santi M, Zeppa SD, Vallorani L, et al. New Insights into the Role of Exercise in Inhibiting mTOR Signaling in Triple-Negative Breast Cancer. Oxid Med Cell Longev. 2018 Sep; 2018: 5896786. DOI: 10.1155/2018/5896786 [DOI] [PubMed]
25. DA Silva Jr ND, Fernandes V, Soci UPR, Monteiro AWA, Phillips MI, DE Oliveira EM. Swimming training in rats increases cardiac MicroRNA-126 expression and angiogenesis. Med Sci Sports Exerc. 2012 Aug; 44(8): 1453-62. DOI: 10.1249/MSS.0b013e31824e8a36 [DOI] [PubMed]
26. Mirdar Sh, Naiestany F, Hamidian Gh, Hedayati M. [Increment of Alveolar Macrophages and Pulmonary Surfactant of Young Male Rats after Six Weeks Interval Training]. Sport Physiology. 2018; 9(36): 59-72. DOI: 10.22089/SPJ.2018.2114.1281 [Article in Persian] [View at Publisher] [DOI]
27. Kodesh E, Zaldivar F, Schwindt C, Tran P, Yu A, Camilon M, et al. A rat model of exercise-induced asthma: a nonspecific response to a specific immunogen. Am J Physiol Regul Integr Comp Physiol. 2011 Apr; 300(4): R917-24. DOI: 10.1152/ajpregu.00270.2010 [DOI] [PubMed]
28. Harijani Mirdar S, Arzani A, Arabzadeh E, Neyestani F, Baghbani M, Ahmadi S. [The Effect of a Period of Interval Training and Step Taper on Performance Indexes in Male Rats during Puberty]. Journal of Sport Biosciences. 2016; 7(4): 619-34. DOI: 10.22059/jsb.2015.57287 [Article in Persian] [View at Publisher] [DOI]
29. Mirdar Sh, Naiestany F, Hamidian Gh, Hedayati M. [Increment of Alveolar Macrophages and Pulmonary Surfactant of Young Male Rats after Six Weeks Interval Training]. Sport Physiology. 2018; 9(36): 59-72. DOI: 10.22089/spj.2018.2114.1281 [Article in Persian] [View at Publisher] [DOI]
30. Nashed BF, Zhang T, Al Alwan M, Srinivasan G, Halayko AJ, Okkenhaug K, et al. Role of the phosphoinositide 3-kinase p110delta in generation of type 2 cytokine responses and allergic airway inflammation. Eur J Immunol. 2007 Feb; 37(2): 416-24. DOI: 10.1002/eji.200636401 [DOI] [PubMed]
31. Cakebread JA, Haitchi HM, Xu Y, Holgate ST, Roberts G, Davies DE. Rhinovirus-16 induced release of IP-10 and IL-8 is augmented by Th2 cytokines in a pediatric bronchial epithelial cell model. PLoS One. 2014 Apr; 9(4): e94010. DOI: 10.1371/journal.pone.0094010 [DOI] [PubMed]
32. Xia X, Hu X, Xu H, Wu L, Dai Y, Yang L, et al. Phosphatidylinositol 3-kinase inhibitor suppresses inducible nitric oxide synthase expression in bronchiole epithelial cells in asthmatic rats. Mol Cell Biochem. 2012 Jan; 359(1-2): 293-99. DOI: 10.1007/s11010-011-1023-y [DOI] [PubMed]
33. Sun G, Li Y, Ji Z. Up-regulation of MIAT aggravates the atherosclerotic damage in atherosclerosis mice through the activation of PI3K/Akt signaling pathway. Drug Deliv. 2019 Dec; 26(1): 641-49. DOI: 10.1080/10717544.2019.1628116 [DOI] [PubMed]
34. Sahiner UM, Birben E, Erzurum S, Sackesen C, Kalayci O. Oxidative stress in asthma. World Allergy Organ J. 2011 Oct; 4(10): 151-58. DOI: 10.1097/WOX.0b013e318232389e [DOI] [PubMed]
35. Qu J, Li Y, Zhong W, Gao P, Hu C. Recent developments in the role of reactive oxygen species in allergic asthma. J Thorac Dis. 2017 Jan; 9(1): E32-E43. DOI: 10.21037/jtd.2017.01.05 [DOI] [PubMed]
36. Baghaiee B, Botelho Teixeira AM, Tartibian B. Moderate aerobic exercise increases SOD-2 gene expression and decreases leptin and malondialdehyde in middle-aged men. Science & Sports. 2016; 31(3): e55-e63. DOI: 10.1016/j.scispo.2015.12.003 [Article] [DOI]
37. Wang Y, Zhou M, Lou W. [Expression and significance of VCAM-1 in subjects with allergic inflammation of respiratory tract]. Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2009 Jun; 23(11): 506-7, 512. [Article in Chinese] [PubMed]
38. Pastva A, Estell K, Schoeb TR, Atkinson TP, Schwiebert LM. Aerobic exercise attenuates airway inflammatory responses in a mouse model of atopic asthma. J Immunol. 2004 Apr; 172(7): 4520-26. DOI: 10.4049/jimmunol.172.7.4520 [DOI] [PubMed]
39. Collison A, Herbert C, Siegle JS, Mattes J, Foster PS, Kumar RK. Altered expression of microRNA in the airway wall in chronic asthma: miR-126 as a potential therapeutic target. BMC Pulm Med. 2011 May; 11: 29. DOI: 10.1186/1471-2466-11-29 [DOI] [PubMed]
40. Hadi H, Gaeini A, Mo'tamedi P, Rajabi H. [The effect of aerobic training on cardiac expression of mir-126 in diabetic rats]. Razi J Med Sci. 2016; 23(148): 44-55. [Article in Persian] [View at Publisher]
41. Harris TA, Yamakuchi M, Kondo M, Oettgen P, Lowenstein CJ. Ets-1 and Ets-2 regulate the expression of microRNA-126 in endothelial cells. Arterioscler Thromb Vasc Biol. 2010 Oct; 30(10): 1990-97. DOI: 10.1161/ATVBAHA.110.211706 [DOI] [PubMed]
42. Yildiz P, Oflaz H, Cine N, Genchallac H, Erginel Ünaltuna N, Yildiz A, et al. Endothelial dysfunction in patients with asthma: the role of polymorphisms of ACE and endothelial NOS genes. J Asthma. 2004 Apr; 41(2): 159-66. DOI: 10.1081/jas-120026073 [DOI] [PubMed]
43. Hergenreider E, Heydt S, Tréguer K, Boettger T, Horrevoets AJG, Zeiher AM, et al. Atheroprotective communication between endothelial cells and smooth muscle cells through miRNAs. Nat Cell Biol. 2012 Feb; 14(3): 249-56. DOI: 10.1038/ncb2441 [DOI] [PubMed]
44. Milkiewicz M, Uchida C, Gee E, Fudalewski T, Haas TL. Shear stress–induced Ets-1 modulates protease inhibitor expression in microvascular endothelial cells. J Cell Physiol. 2008 Nov; 217(2): 502-10. DOI: 10.1002/jcp.21526 [DOI] [PubMed]
45. Tinken TM, Thijssen DHJ, Hopkins N, Dawson EA, Cable NT, Green DJ. Shear stress mediates endothelial adaptations to exercise training in humans. Hypertension. 2010 Feb; 55(2): 312-18. DOI: 10.1161/HYPERTENSIONAHA.109.146282 [DOI] [PubMed]
46. Garcia-Lacarte M, Mansego ML, Zulet MA, Martinez JA, Milagro FI. miR-1185-1 and miR-548q Are Biomarkers of Response to Weight Loss and Regulate the Expression of GSK3B. Cells. 2019 Nov; 8(12): 1548. DOI: 10.3390/cells8121548 [DOI] [PubMed]
47. Pallarès-Albanell J, Zomeño-Abellán MT, Escaramís G, Pantano L, Soriano A, Segura MF, et al. A High-Throughput Screening Identifies MicroRNA Inhibitors That Influence Neuronal Maintenance and/or Response to Oxidative Stress. Mol Ther Nucleic Acids. 2019 Sep; 17: 374-87. DOI: 10.1016/j.omtn.2019.06.007 [DOI] [PubMed]
Send email to the article author

XML   Persian Abstract   Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Nobahar M, Siahkouhian M, Mirdar S. A survey of moderate interval training on expression of mir-126, mir-1185 AKT and VCAM in cardiac tissue of rats with allergic asthma. J Gorgan Univ Med Sci 2021; 23 (1) :55-63
URL: http://goums.ac.ir/journal/article-1-3781-en.html
Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 23, Issue 1 (3-2021) Back to browse issues page
مجله دانشگاه علوم پزشکی گرگان Journal of Gorgan University of Medical Sciences
Persian site map - English site map - Created in 0.05 seconds with 36 queries by YEKTAWEB 4660
Creative Commons License
This work is licensed under a Creative Commons — Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)