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Evaluation of enterohemorrhagic Escherichia coli of outbreaks of foodborne disease by molecular method
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Mohammad Mehdi Soltan Dallal *    1, Omolbanin Biglari2 , Zahra Rajabi3 , Mohammad Kazem Sharifi Yazdi4 , Abbas Rahimi Foroushani5 , Shabnam Haghighat Khajavi6 |
1- Professor, Food Microbiology Research Center/ Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. , msoltandallal@gmail.com
2- M.Sc Student of Food Microbiology, Department of Pathobiology, Faculty of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
3- Ph.D Candidate in Microbiology, Department of Microbiology, University of Alzahra / Food Microbiology Research Center, Tehran University of Medical Sciences, Tehran, Iran.
4- Professor of Zoonosis Research Centre, Department of Medical Laboratory Sciences, Faculty of Allied Medical Sciences, Tehran University of Medical Sciences, Tehran, Iran.
5- Professor, Department of Epidemiology and Biostatistics, School of PublicHealth, Tehran University of Medical Sciences, Tehran, Iran.
6- Assistant professor, Department of Food Science and Technology, Science and Research Branch, Islamic Azad University,Tehran,Iran. |
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Abstract: (8920 Views) |
Background and Objective: The most common enterohemorrhagic Escherichia coli strain is the O157: H7 serotype, which is one of the most important intestinal pathogens and can cause complications such as hemorrhagic colitis, hemolytic uremic syndrome and acute renal failure. The aim of this study was to evaluate the enterohemorrhagic Escherichia coli causing molecular outbreaks of foodborne illness in Iran.
Methods: In this descriptive cross-sectional study, 189 fecal swab specimens were examined during April to September 2018. All suspected isolates were tested for biochemical tests. The isolates were confirmed by molecular PCR and evaluated by antimicrobial susceptibility tests.
Results: From 189 stool swab samples studied, 98 Escherichia coli isolates were detected based on phenotypic tests. Most of the outbreaks occurred in summer and the prevalence of enterohemorrhagic Escherichia coli was 24.5%, which 4% of them were non-O157H7. Most patients were between 1 and 12 years of age and the highest antibiotic resistance to cotrimoxazole and chloramphenicol was observed at 80% and 79%, respectively.
Conclusion: This study showed an increase in enterohemorrhagic Escherichia coli with 24.5% and an increase in antibiotic resistance to the antibiotics of chloramphenicol, cotrimoxazole and carbapenems. Increased resistance to imipenem and meropenem antibiotics makes it difficult to treat beta-lactamase-resistant strains.
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Keywords: Enterohemorrhagic Escherichia coli [MeSH], Disease Outbreaks [MeSH], Foodborne Disease [MeSH]
Article ID: Vol23-14 |
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Full-Text [PDF 735 kb]
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Type of Study: Original Articles |
Subject:
Microbiology
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Soltan Dallal M M, Biglari O, Rajabi Z, Sharifi Yazdi M K, Rahimi Foroushani A, Haghighat Khajavi S. Evaluation of enterohemorrhagic Escherichia coli of outbreaks of foodborne disease by molecular method. J Gorgan Univ Med Sci 2021; 23 (1) :108-115 URL: http://goums.ac.ir/journal/article-1-3756-en.html
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