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:: Volume 22, Issue 1 (3-2020) ::
J Gorgan Univ Med Sci 2020, 22(1): 57-64 Back to browse issues page
Effect of Diazepam on testicular tissue parameters and spermatogenesis in rats
Amir Bagher Ilkhani1 , Maryam Tehranipour * 2, Saeedeh Zafar Balanezhad3
1- M.Sc in Animal Biology, Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran.
2- Associate Professor, Department of Biology, Mashhad Branch, Islamic Azad University of Medical Sciences, Mashhad, Iran , maryam_tehranipour@mshdiau.ac.ir
3- Assistant Professor, Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran.
Abstract:   (10406 Views)
Background and Objective: Sperm dysfunction and damage in spermatogenesis are the most common causes of male infertility. Diazepam is also a painkiller for benzodiazepines that can be addictive for a long time. This study was done to determine the effect of Diazepam on testicular tissue parameters and spermatogenesis in Rats.
Methods: In this experimental study, 30 Wistar male rats with a 250-200 gram weight were randomly allocated into 5 groups. Experimental groups were received diazepam with doses of (2, 3, 4, 5 mg/kg/bw) for 14 days, intraperitonally. Serum physiology was injected in control group. The animals were anesthetized and the testes and epididymis ductus defran were removed for examination, sperm motility, and percentage of live sperm.
Results: Weight, large and small testicular diameter, percentage of live sperm and number of sperm moving forward were reduced with injection groups, at a dose of 3 mg / kg in all factors except the number of sperm moving forward in compared to the control group. In other groups, only testicular weight was significantly reduced at a dose of 2 mg/kg (P<0.05).
Conclusion: Diazepam can affect spermatogenesis process in rats.
Keywords: Diazepam, Spermatogenesis, Fertility, Rat
Article ID: Vol22-08
Full-Text [PDF 418 kb]   (13005 Downloads)    
Type of Study: Original Articles | Subject: Physiology - Pharmacology
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Ilkhani A B, Tehranipour M, Zafar Balanezhad S. Effect of Diazepam on testicular tissue parameters and spermatogenesis in rats. J Gorgan Univ Med Sci 2020; 22 (1) :57-64
URL: http://goums.ac.ir/journal/article-1-3512-en.html

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Volume 22, Issue 1 (3-2020) Back to browse issues page
مجله دانشگاه علوم پزشکی گرگان Journal of Gorgan University of Medical Sciences
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