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:: Volume 20, Issue 3 (10-2018) ::
J Gorgan Univ Med Sci 2018, 20(3): 31-39 Back to browse issues page
Comparison the effect of eight weeks aerobic training with moderate and high intensities on serum levels of Irisin and Uncoupling Protein 1 (UCP-1) in white adipose tissue in obese male rats
Keyvan Hejazi1 , Seyyed Reza Attarzadeh Hosseini * 2, Mehrdad Fathi3 , Mohammad Mosaferi Ziaaldini4 , Mahdieh Zaeemi5
1- Ph.D Candidate in Sport Physiology, Faculty of Sport Sciences, Ferdowsi University of Mashhad, Mashhad, Iran
2- Professor in Sport Physiology, Faculty of Sports Sciences, Ferdowsi University of Mashhad, Mashhad, Iran. attarzadeh@um.ac.ir , attarzadeh@um.ac.ir
3- Associate Professor in Sport Physiology, Faculty of Sports Sciences, Ferdowsi University of Mashhad, Mashhad, Iran
4- Assistant Professor in Sport Physiology, Faculty of Sports Sciences, Ferdowsi University of Mashhad, Mashhad, Iran
5- Assistant Professor, Department of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
Abstract:   (7494 Views)
Background and Objective: The synthesis of uncoupling protein 1 (UCP1) in adipose tissue plays an important role in providing resistance and prevention of fat accumulation, weight gain and obesity. This study was done to evaluate the effect of eight weeks aerobic training with moderate and high intensities on serum levels of Irisin and UCP1 white adipose tissue in obese male rats.
Methods: In this experimental study, 24 adult obese male Wistar rats (weight: 250 to 300 gr, BMI>30g/cm2) were randomly assigned into three groups including moderate aerobic training intensity, high intensity aerobic training and control group. The aerobic exercise training was included 8 weeks (5 sessions/per-week for 60 min per session). All training groups carried out aerobic training with 28 m/min (moderate intensity), aerobic training with 34 m/min (high intensity) on treadmill. 48 hours after the training period, the level of UCP1 and Irisin protein was measured.
Results: The level of UCP-1 in adipose tissue and serum Irisin in both aerobic training intensities increased compared to control group, but this increase only in aerobic training group with a moderate intensity was singnificant (p<0.05).
Conclusion: Eight weeks aerobic training with moderate intensity leads to increase of UCP-1 in adipose tissue and Irisin levels.
Keywords: Aerobic training, Irisin, Uncoupling protein 1, White adipose tissue
Full-Text [PDF 330 kb] [English Abstract]   (12243 Downloads) |   |   Abstract (HTML)  (267 Views)  
Type of Study: Original Articles | Subject: Exercise Physiology
* Corresponding Author Address: Professor in Sport Physiology, Faculty of Sports Sciences, Ferdowsi University of Mashhad, Mashhad, Iran. attarzadeh@um.ac.ir
1. Ng M, Fleming T, Robinson M, Thomson B, Graetz N, Margono C, et al. Global, regional, and national prevalence of overweight and obesity in children and adults during 1980-2013: a systematic analysis for the Global Burden of Disease Study 2013. Lancet. 2014 Aug; 384(9945): 766-81. doi:10.1016/S0140-6736(14)60460-8
2. Guyenet SJ, Schwartz MW. Clinical review: Regulation of food intake, energy balance, and body fat mass: implications for the pathogenesis and treatment of obesity. J Clin Endocrinol Metab. 2012 Mar; 97(3): 745-55. doi:10.1210/jc.2011-2525
3. García-Hermoso A, Cerrillo-Urbina AJ, Herrera-Valenzuela T, Cristi-Montero C, Saavedra JM, Martínez-Vizcaíno V. Is high-intensity interval training more effective on improving cardiometabolic risk and aerobic capacity than other forms of exercise in overweight and obese youth? A meta-analysis. Obes Rev. 2016 Jun; 17(6): 531-40. doi:10.1111/obr.12395
4. Granneman JG, Li P, Zhu Z, Lu Y. Metabolic and cellular plasticity in white adipose tissue I: effects of beta3-adrenergic receptor activation. Am J Physiol Endocrinol Metab. 2005 Oct; 289(4): E608-16. doi:10.1152/ajpendo.00009.2005
5. Ruas JL, White JP, Rao RR, Kleiner S, Brannan KT, Harrison BC, et al. A PGC-1alpha isoform induced by resistance training regulates skeletal muscle hypertrophy. Cell. 2012 Dec; 151(6): 1319-31. doi:10.1016/j.cell.2012.10.050
6. Virtue S, Vidal-Puig A. Adipose tissue expandability, lipotoxicity and the Metabolic Syndrome--an allostatic perspective. Biochim Biophys Acta. 2010 Mar; 1801(3): 338-49. doi:10.1016/j.bbalip.2009.12.006
7. Cannon B, Nedergaard J. Brown adipose tissue: function and physiological significance. Physiol Rev. 2004 Jan; 84(1): 277-359. doi:10.1152/physrev.00015.2003
8. Goldwasser J, Cohen PY, Yang E, Balaguer P, Yarmush ML, Nahmias Y. Transcriptional regulation of human and rat hepatic lipid metabolism by the grapefruit flavonoid naringenin: role of PPARalpha, PPARgamma and LXRalpha. PLoS One. 2010 Aug; 5(8): e12399. doi:10.1371/journal.pone.0012399
9. Chen M, Norman RJ, Heilbronn LK. Does in vitro fertilisation increase type 2 diabetes and cardiovascular risk? Curr Diabetes Rev. 2011 Nov; 7(6): 426-32.
10. Medina-Gomez G, Gray S, Vidal-Puig A. Adipogenesis and lipotoxicity: role of peroxisome proliferator-activated receptor gamma (PPARgamma) and PPARgammacoactivator-1 (PGC1). Public Health Nutr. 2007 Oct; 10(10A): 1132-37. doi:10.1017/S1368980007000614
11. Dalgaard LT, Pedersen O. Uncoupling proteins: functional characteristics and role in the pathogenesis of obesity and Type II diabetes. Diabetologia. 2001 Aug; 44(8): 946-65. doi:10.1007/s001250100596
12. Schrauwen P, Walder K, Ravussin E. Human uncoupling proteins and obesity. Obes Res. 1999 Jan; 7(1): 97-105.
13. Feldmann HM, Golozoubova V, Cannon B, Nedergaard J. UCP1 ablation induces obesity and abolishes diet-induced thermogenesis in mice exempt from thermal stress by living at thermoneutrality. Cell Metab. 2009 Feb; 9(2): 203-9. doi:10.1016/j.cmet.2008.12.014
14. Brondani LA, Assmann TS, Duarte GC, Gross JL, Canani LH, Crispim D. The role of the uncoupling protein 1 (UCP1) on the development of obesity and type 2 diabetes mellitus. Arq Bras Endocrinol Metabol. 2012 Jun; 56(4): 215-25.
15. Lee P, Greenfield JR. Non-pharmacological and pharmacological strategies of brown adipose tissue recruitment in humans. Mol Cell Endocrinol. 2015 Dec; 418 Pt 2: 184-90. doi:10.1016/j.mce.2015.05.025
16. Wu J, Cohen P, Spiegelman BM. Adaptive thermogenesis in adipocytes: is beige the new brown? Genes Dev. 2013 Feb; 27(3): 234-50. doi:10.1101/gad.211649.112
17. Bonet ML, Oliver P, Palou A. Pharmacological and nutritional agents promoting browning of white adipose tissue. Biochim Biophys Acta. 2013 May; 1831(5): 969-85. doi:10.1016/j.bbalip.2012.12.002
18. Boström P, Wu J, Jedrychowski MP, Korde A, Ye L, Lo JC, et al. A PGC1-alpha-dependent myokine that drives brown-fat-like development of white fat and thermogenesis. Nature. 2012 Jan; 481(7382): 463-68. doi:10.1038/nature10777
19. Daneshyar S, Kordi MR, Gaeini AA, Kadivar M, Afshari S. [The effect of endurance training on gene expression of uncoupling protein 1(UCP-1) in white visceral adipose tissue of retroperitoneal depot of male Wistar rats]. Razi J Med Sci. 2015; 22(136): 35-45. [Article in Persian]
20. Reisi J, Ghaedi K, Rajabi H, Marandi SM. Can resistance exercise alter irisin levels and expression profiles of FNDC5 and UCP1 in rats? Asian J Sport Med. 2016; 7(4): e35205. doi:10.5812/asjsm.35205
21. Vílchez-López FJ, Rivas-Rivas M, Mateo-Gavira I, Rodríguez-Ramos C, Romero-Gómez M. Aerobic exercise increases catalase activity and decreased levels of leptin and TNF [alpha] in patients with liver disease. Endocrine Abstracts. 18th European Congress of Endocrinology. 28–31 May 2016, Munich, Germany. 2016; 41: EP213. doi:10.1530/endoabs.41.EP213
22. Pekkala S, Wiklund PK, Hulmi JJ, Ahtiainen JP, Horttanainen M, Pöllänen E, et al. Are skeletal muscle FNDC5 gene expression and irisin release regulated by exercise and related to health? J Physiol. 2013 Nov; 591(21): 5393-400. doi:10.1113/jphysiol.2013.263707
23. Huh JY, Mougios V, Kabasakalis A, Fatouros I, Siopi A, Douroudos II, et al. Exercise-induced irisin secretion is independent of age or fitness level and increased irisin may directly modulate muscle metabolism through AMPK activation. J Clin Endocrinol Metab. 2014 Nov; 99(11): E2154-61. doi:10.1210/jc.2014-1437
24. Afshari S, Mohammad-Amoli M, Daneshyar S. [Comparison of moderate and high volume aerobic training on gene expression of uncoupling protein 1 (UCP-1) in subcutaneous white adipose tissue of wistar rats]. Int J Endocrinol Metab. 2017; 19(1): 34-40. [Article in Persian]
25. Novelli EL, Diniz YS, Galhardi CM, Ebaid GM, Rodrigues HG, Mani F, et al. Anthropometrical parameters and markers of obesity in rats. Lab Anim. 2007 Jan; 41(1): 111-19. doi:10.1258/002367707779399518
26. Garekani ET, Mohebbi H, Kraemer RR, Fathi R. Exercise training intensity/volume affects plasma and tissue adiponectin concentrations in the male rat. Peptides. 2011 May; 32(5): 1008-12. doi:10.1016/j.peptides.2011.01.027
27. Jashni HK, Mohebbi H, Delpasand A, Jahromy HK. Caloric restriction and exercise training, combined, not solely improve total plasma adiponectin and glucose homeostasis in streptozocin-induced diabetic rats. Sport Sciences for Health. 2015; 11(1): 81-86.
28. Shepherd RE, Gollnick PD. Oxygen uptake of rats at different work intensities. Pflugers Arch. 1976 Apr; 362(3): 219-22.
29. Lee S, Farrar RP. Resistance training induces muscle-specific changes in muscle mass and function in rat. J Exerc Physiol Online. 2003; 6(2): 80-87.
30. Khalafi M, Ravasi A A, Shabkhiz F, Moradi M, Zarei Y. [The effects of high intensity interval exercise (hiie) and moderate intensity continuous exercise (mice) on serum irisin and subcutaneous ucp-1 in diabetic male rats]. Iran J Diabetes Metabol. 2016; 15(4): 237-46. [Article in Persian]
31. Nedergaard J, Cannon B. The browning of white adipose tissue: some burning issues. Cell Metab. 2014 Sep; 20(3): 396-407. doi:10.1016/j.cmet.2014.07.005
32. Ringholm S, Grunnet Knudsen J, Leick L, Lundgaard A, Munk Nielsen M, Pilegaard H. PGC-1alpha is required for exercise- and exercise training-induced UCP1 up-regulation in mouse white adipose tissue. PLoS One. 2013 May; 8(5): e64123. doi:10.1371/journal.pone.0064123
33. González GJ, López Ruiz E, Griñán-Lisón C, Antonio Marchal J. Brown adipose tissue and obesity. Obesity and Thyroid Cancer. 2016; 1(1): 13-28. doi:10.1007/978-3-319-19821-7_2
34. Fulmer T. Burning brown fat. SciBX: Science-Business eXchange. 2010; 3(20): 605-6.
35. Borer K. Advanced exercise endocrinology. 1st ed. Champaign: Human Kinetics. 2013; pp: 163-70.
36. Borer K. Exercise endocrinology. 1st ed. Champaign: Human Kinetics. 2003; pp: 152-55.
37. Joost HG, Weber TM, Cushman SW. Qualitative and quantitative comparison of glucose transport activity and glucose transporter concentration in plasma membranes from basal and insulin-stimulated rat adipose cells. Biochem J. 1988; 249(1): 155-61.
38. Rizza RA, Gerich JE, Haymond MW, Westland RE, Hall LD, Clemens AH, et al. Control of blood sugar in insulin-dependent diabetes: comparison of an artificial endocrine pancreas, continuous subcutaneous insulin infusion, and intensified conventional insulin therapy. N Engl J Med. 1980 Dec; 303(23): 1313-18.
39. Kim J, Saidel GM, Kalhan SC. A computational model of adipose tissue metabolism: evidence for intracellular compartmentation and differential activation of lipases. J Theor Biol. 2008 Apr; 251(3): 523-40. doi:10.1016/j.jtbi.2007.12.005
40. Lafontan M. [Lipid metabolism in adipose tissue: adrenergic control of the adipocyte and mobilization if the lipids]. Ann Endocrinol (Paris). 2001 Sep; 62(4 Pt 2): S7-17. [Article in French]
41. Symonds ME. Adipose tissue biology. New York: Springer. 2012; pp: 88-92.
42. Huh JY, Siopi A, Mougios V, Park KH, Mantzoros CS. Irisin in response to exercise in humans with and without metabolic syndrome. J Clin Endocrinol Metab. 2015 Mar; 100(3): E453-7. doi:10.1210/jc.2014-2416
43. Winn NC, Grunewald ZI, Liu Y, Heden TD, Nyhoff LM, Kanaley JA. Plasma irisin modestly increases during moderate and high-intensity afternoon exercise in obese females. PLoS One. 2017; 12(1):e0170690. doi:10.1371/journal.pone.0170690
44. Daskalopoulou SS, Cooke AB, Gomez YH, Mutter AF, Filippaios A, Mesfum ET, et al. Plasma irisin levels progressively increase in response to increasing exercise workloads in young, healthy, active subjects. Eur J Endocrinol. 2014 Sep; 171(3): 343-52. doi:10.1530/EJE-14-0204
45. Kim HJ, Lee HJ, So B, Son JS, Yoon D, Song W. Effect of aerobic training and resistance training on circulating irisin level and their association with change of body composition in overweight/obese adults: a pilot study. Physiol Res. 2016 Jun; 65(2): 271-79.
46. Huh JY, Panagiotou G, Mougios V, Brinkoetter M, Vamvini MT, Schneider BE, et al. FNDC5 and irisin in humans: I. Predictors of circulating concentrations in serum and plasma and II. mRNA expression and circulating concentrations in response to weight loss and exercise. Metabolism. 2012 Dec; 61(12): 1725-38. doi:10.1016/j.metabol.2012.09.002
47. Roca-Rivada A, Castelao C, Senin LL, Landrove MO, Baltar J, Belén Crujeiras A, et al. FNDC5/irisin is not only a myokine but also an adipokine. PLoS One. 2013 Apr; 8(4): e60563. doi:10.1371/journal.pone.0060563
48. Norheim F, Langleite TM, Hjorth M, Holen T, Kielland A, Stadheim HK, et al. The effects of acute and chronic exercise on PGC-1, irisin and browning of subcutaneous adipose tissue in humans. FEBS J. 2014 Feb; 281(3): 739-49. doi:10.1111/febs.12619
49. Lecker SH, Zavin A, Cao P, Arena R, Allsup K, Daniels KM, et al. Expression of the irisin precursor FNDC5 in skeletal muscle correlates with aerobic exercise performance in patients with heart failure. Circ Heart Fail. 2012 Nov; 5(6): 812-18. doi:10.1161/CIRCHEARTFAILURE.112.969543
50. Suwa M, Nakano H, Radak Z, Kumagai S. Endurance exercise increases the SIRT1 and peroxisome proliferator-activated receptor gamma coactivator-1alpha protein expressions in rat skeletal muscle. Metabolism. 2008 Jul; 57(7): 986-98. doi:10.1016/j.metabol.2008.02.017
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Hejazi K, Attarzadeh Hosseini S R, Fathi M, Mosaferi Ziaaldini M, Zaeemi M. Comparison the effect of eight weeks aerobic training with moderate and high intensities on serum levels of Irisin and Uncoupling Protein 1 (UCP-1) in white adipose tissue in obese male rats. J Gorgan Univ Med Sci 2018; 20 (3) :31-39
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Volume 20, Issue 3 (10-2018) Back to browse issues page
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