[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Home::
Journal Information::
About the Journal
Aim and Scope
Content Coverage
Ethics
Conflict of Interest
Copyrights & License
Price
Indexing Sources::
Editorial Board::
Executive Members::
Articles Archive::
Current Issue
All Issues
Instruction to Authors::
Peer-Review::
Contact Us::
Contact Us
Contact Form
Site Facilities::
Search contents
::
Search in website

Advanced Search
Receive site information
Enter your Email in the following box to receive the site news and information.
:: Volume 20, Issue 2 (7-2018) ::
J Gorgan Univ Med Sci 2018, 20(2): 35-41 Back to browse issues page
Effect of exogenous estrogen treatment on hippocampal neurogenesis in ovariectomized mice
Maryam Azari1 , Mohammad Taghi Ghorbanian * 2, Mahmoud Elah Dadi Salmani3
1- M.Sc in Histology, School of Biology and Institute of Biological Sciences, Damghan University, Damghan, Iran
2- Assistant Professor, Depatment of Cellular and Molecular Biology, School of Biology and Institute of Biological Sciences, Damghan University, Damghan, Iran. ghorbanian@du.ac.ir , ghorbanian@du.ac.ir
3- Associate Professor, Department of Biology, School of Biology and Institute of Biological Sciences, Damghan University, Damghan, Iran
Abstract:   (10526 Views)
Background and Objective: Adult neurogenesis occurs in most mammalian species in two main areas of brain: 1- subventricular zone 2- the dentate gyrus of the hippocampus. Many factors such as 17-B estradiol affect neurogenesis in the hippocampus. The aim of this study was to investigate the effect of exogenous 17-B estradiol on neurogenesis and astrocyte functions in the ovariectomized mice.
Methods: In this experimental study; NMRI mice were allocated into five experimental groups including Sham, Control, Treatment with single dose of 17-B estradiol two weeks after ovariectomy (OVX) and were sacrificed 24 hours later, Treatment with single dose of 17-B estradiol two weeks after Ovx and were sacrificed 48 hours later and   Treatment with single dose of Seasame Oil 2 weeks after OVX and were sacrificed after 24 hours. Animals were transcardially perfused with paraformaldehyde. Brains were removed and its sections for cresyl fast violet staining and GFAP immunohistochemistry were prepared. Cells were counted and investigated.
Results: Neuronal density and Proliferation of hippocampal progenitor cells in the CA1 region of 17-B estradiol treated mice significantly increased up to 24 hours (P<0.05). Density of glia and particularly astrocytes in different regions of the hippocampus significantly reduced after treatment with 17-B estradiol (P<0.05).
Conclusion: Density of hippocampal CA1 neurons are influenced by 17-B estradiol. Also, density and morphology of glia cells, especially astrocytes in different regions of the hippocampus are affected by 17-B estradiol.
Keywords: 17-B estradiol, Neurogenesis, Hippocampus, Astrocyte, Mouse
Full-Text [PDF 377 kb] [English Abstract]   (12985 Downloads) |   |   Abstract (HTML)  (356 Views)  
Type of Study: Original Articles | Subject: Neurosciences
* Corresponding Author Address: Assistant Professor, Depatment of Cellular and Molecular Biology, School of Biology and Institute of Biological Sciences, Damghan University, Damghan, Iran. ghorbanian@du.ac.ir
References
1. Alenzi FQB, Bahkali AH. Stem cells: biology and clinical potential. Afr J Biotechnol. 2011; 10(86): 19929-40. doi:10.5897/AJBX11.046
2. Kordower JH, Chen EY, Morrison JH. Long-term gonadal hormone treatment and endogenous neurogenesis in the dentate gyrus of the adult female monkey. Exp Neurol. 2010 Jul; 224(1): 252-57. doi:10.1016/j.expneurol.2010.03.027
3. Pino A, Fumagalli G, Bifari F, Decimo I. New neurons in adult brain: distribution, molecular mechanisms and therapies. Biochem Pharmacol. 2017 Oct; 141: 4-22. doi:10.1016/j.bcp.2017.07.003
4. Lennington JB, Yang Z, Conover JC. Neural stem cells and the regulation of adult neurogenesis. Reprod Biol Endocrinol. 2003 Nov; 1: 99. doi:10.1186/1477-7827-1-99
5. Brock O, Keller M, Veyrac A, Douhard Q, Bakker J. Short term treatment with estradiol decreases the rate of newly generated cells in the subventricular zone and main olfactory bulb of adult female mice. Neuroscience. 2010 Mar; 166(2): 368-76. doi:10.1016/j.neuroscience.2009.12.050
6. Wnuk A, Korol DL, Erickson KI. Estrogens, hormone therapy, and hippocampal volume in postmenopausal women. Maturitas. 2012 Nov; 73(3): 186-90. doi:10.1016/j.maturitas.2012.07.001
7. Zhao C, Deng W, Gage FH. Mechanisms and functional implications of adult neurogenesis. Cell. 2008 Feb; 132(4): 645-60. doi:10.1016/j.cell.2008.01.033
8. Pawluski JL, Brummelte S, Barha CK, Crozier TM, Galea LA. Effects of steroid hormones on neurogenesis in the hippocampus of the adult female rodent during the estrous cycle, pregnancy, lactation and aging. Front Neuroendocrinol. 2009 Aug; 30(3): 343-57. doi:10.1016/j.yfrne.2009.03.007
9. Seki T. Microenvironmental elements supporting adult hippocampal neurogenesis. Anat Sci Int. 2003 Jun; 78(2): 69-78. doi:10.1046/j.0022-7722.2003.00043.x
10. Magavi SS, Leavitt BR, Macklis JD. Induction of neurogenesis in the neocortex of adult mice. Nature. 2000 Jun; 405(6789): 951-55. doi:10.1038/35016083
11. Gould E, Gross CG. Neurogenesis in adult mammals: some progress and problems. J Neurosci. 2002 Feb; 22(3): 619-23.
12. Taupin P. Neurogenesis in the adult central nervous system. C R Biol. 2006 Jul; 329(7): 465-75. doi:10.1016/j.crvi.2006.04.001
13. Saravia F, Beauquis J, Pietranera L, De Nicola AF. Neuroprotective effects of estradiol in hippocampal neurons and glia of middle age mice. Psychoneuroendocrinology. 2007 Jun; 32(5): 480-92. doi:10.1016/j.psyneuen.2007.02.012
14. Sauerzweig S, Baldauf K, Braun H, Reymann KG. Time-dependent segmentation of BrdU-signal leads to late detection problems in studies using BrdU as cell label or proliferation marker. J Neurosci Methods. 2009 Feb; 177(1): 149-59. doi:10.1016/j.jneumeth.2008.10.009
15. Ormerod BK, Lee TT, Galea LA. Estradiol initially enhances but subsequently suppresses (via adrenal steroids) granule cell proliferation in the dentate gyrus of adult female rats. J Neurobiol. 2003 May; 55(2): 247-60. doi:10.1002/neu.10181
16. Horner PJ, Palmer TD. New roles for astrocytes: the nightlife of an 'astrocyte'. La vida loca! Trends Neurosci. 2003 Nov; 26(11): 597-603. doi:10.1016/j.tins.2003.09.010
17. Faigle R, Song H. Signaling mechanisms regulating adult neural stem cells and neurogenesis. Biochim Biophys Acta. 2013 Feb; 1830(2): 2435-48. doi:10.1016/j.bbagen.2012.09.002
18. Ying Zhu, Quanguang Zhang, Wenli Zhang, Ning Li, Yongxin Dai, Jingyi Tu, et al. Protective effect of 17β-Estradiol upon hippocampal spine density and cognitive function in an animal model of vascular dementia. Sci Rep. 2017; 7: 42660. doi:10.1038/srep42660
19. Lie DC, Song H, Colamarino SA, Ming GL, Gage FH. Neurogenesis in the adult brain: new strategies for central nervous system diseases. Annu Rev Pharmacol Toxicol. 2004; 44:399-421. doi:10.1146/annurev.pharmtox.44.101802.121631
20. Sriraksa N, Wattanathorn J, Muchimapura S, Tiamkao S, Brown K, Chaisiwamongkol K. Cognitive-enhancing effect of quercetin in a rat model of Parkinson's disease induced by 6-hydroxydopamine. Evidence-Based Complementary and Alternative Medicine. Volume 2012, Article ID 823206. http://dx.doi.org/10.1155/2012/823206
21. Suhonen JO, Peterson DA, Ray J, Gage FH. Differentiation of adult hippocampus-derived progenitors into olfactory neurons in vivo. Nature. 1996 Oct; 383(6601): 624-27. doi:10.1038/383624a0
22. Song H, Stevens CF, Gage FH. Astroglia induce neurogenesis from adult neural stem cells. Nature. 2002 May; 417(6884): 39-44. doi:10.1038/417039a
23. Palmer TD, Willhoite AR, Gage FH. Vascular niche for adult hippocampal neurogenesis. J Comp Neurol. 2000 Oct; 425(4): 479-94.
24. Shen Q, Goderie SK, Jin L, Karanth N, Sun Y, Abramova N, et al. Endothelial cells stimulate self-renewal and expand neurogenesis of neural stem cells. Science. 2004 May; 304(5675): 1338-40. doi:10.1126/science.1095505
25. Sadrollahi M, Ghorbanian M T, Zavareh S. [Hippocampal neurogenesis in mice at different phases of the estrous cycle]. Feyz. 2014; 18(4): 336-44. [Article in Persian]
26. Heberden C. Sex steroids and neurogenesis. Biochem Pharmacol. 2017 Oct; 141: 56-62. doi:10.1016/j.bcp.2017.05.019
27. Etgen AM. Estrogen regulation of neurotransmitter and growth factor signaling in the brain. Hormones, Brain and Behavior. 2002; 3: 381-440. https://doi.org/10.1016/B978-012532104-4/50048-2
Send email to the article author

XML   Persian Abstract   Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Azari M, Ghorbanian M T, Dadi Salmani M E. Effect of exogenous estrogen treatment on hippocampal neurogenesis in ovariectomized mice . J Gorgan Univ Med Sci 2018; 20 (2) :35-41
URL: http://goums.ac.ir/journal/article-1-3377-en.html
Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 20, Issue 2 (7-2018) Back to browse issues page
مجله دانشگاه علوم پزشکی گرگان Journal of Gorgan University of Medical Sciences
Persian site map - English site map - Created in 0.08 seconds with 36 queries by YEKTAWEB 4657