[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Home::
Journal Information::
Indexing Sources::
Editorial Board::
Executive Members::
Articles Archive::
Instruction to Authors::
Peer-Review::
Contact Us::
Site Facilities::
::
Search in website

Advanced Search
Receive site information
Enter your Email in the following box to receive the site news and information.
:: Volume 20, Issue 2 (7-2018) ::
J Gorgan Univ Med Sci 2018, 20(2): 102-108 Back to browse issues page
Effect of methanolic extract of Nasturtium officinale on growth and biofilm formation of Pseudomonas aeruginosa
Aryan Omidi1 , Asghar Sharifi * 2
1- M.Sc in Microbiology, Young Researchers and Elite Club, Yasooj Branch, Islamic Azad University, Yasooj, Iran
2- Associate Professor, Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran. asgharsharifi@yahoo.com , asgharsharifi@yahoo.com
Abstract:   (8532 Views)

Background and Objective: Biofilms caused by pathogenic microorganisms that plays an important role against human health. Due to their resistance to detergents and antimicrobial agent, treatment response of affected patients with these bacteria is difficult. This study was done to evaluate the effect of methanol extract of Nasturtium officinale plant on growth and biofilm formation of Pseudomonas aeruginosa.
Methods: In this descriptive - laboratory study, the extraction was done by Maceration in 80% methanol and by rotary evaporator. The minimum inhibitory concentration (MIC) of Nasturtium officinale extracts were determined by broth microdilution method. Biofilm formation was investigated using the microtiter plate and stained with crystal violet.
Results: The minimum inhibitory concentration of Nasturtium officinale against Pseudomonas aeruginosa was 0.625 mg/ml and the Minimum bactericidal concentration of this extract was 1.25 mg/ml. PAO1 strain and 5 clinical strains were able to biofilm formation. Inhibition of biofilm formation by extract of Nasturtium officinale plant was dependent to concentration. The highest percentage of inhibition of biofilm formation was in the concentration of 7.5 mg/ml and the lowest percentage of inhibition of biofilm formation was in the concentration of 0.11 mg/ml. The mean of Pseudomonas aeruginosa biofilm inhibition by Nasturtium officinale extracts was 72.69±22.27 %. In the concentrations of 7.5, 0.93, 0.46, 0.23 and 0.11 mg/ml, there was a significant difference between clinical strains and PAO1 strain (P<0.05).
Conclusion: Methanolic extracts of Nasturtium officinale plant has anti-bacterial and anti-biofilm effect against Pseudomonas aeruginosa.

Keywords: Nasturtium officinale, Pseudomonas aeruginosa, Biofilm, MIC
Full-Text [PDF 286 kb] [English Abstract]   (14136 Downloads) |   |   Abstract (HTML)  (324 Views)  
Type of Study: Original Articles | Subject: Microbiology
* Corresponding Author Address: Associate Professor, Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran. asgharsharifi@yahoo.com
References
1. Streeter K, Katouli M. Pseudomonas aeruginosa: A review of their Pathogenesis and Prevalence in Clinical Settings and the Environment. Infection Epidemiology and Microbiology. 2016; 2 (1) :25-32.
2. Karatuna O, Yagci A. Analysis of quorum sensing-dependent virulence factor production and its relationship with antimicrobial susceptibility in Pseudomonas aeruginosa respiratory isolates. Clin Microbiol Infect. 2010 Dec; 16(12): 1770-5. doi:10.1111/j.1469-0691.2010.03177.x
3. Shaikh S, Fatima J, Shakil S, Rizvi SM, Kamal MA. Prevalence of multidrug resistant and extended spectrum beta-lactamase producing Pseudomonas aeruginosa in a tertiary care hospital. Saudi J Biol Sci. 2015; 22(1): 62-64.
4. Adonizio A, Kong K.F, Mathee K. Inhibition of quorum sensing-controlled virulence factuor production in Pseudomonas aeruginosa by South Florida plant extracts. Antimicrob Agents Chemother. 2008; 52(1): 198-203.
5. Gellatly SL, Hancock RE. Pseudomonas aeruginosa: new insights into pathogenesis and host defenses. Pathog Dis. 2013 Apr; 67(3): 159-73. doi:10.1111/2049-632X.12033
6. Ghotaslou R, Salahi Eshlaqghi B. [Biofilm of Pseudomonas aeruginosa and new preventive measures and anti-biofilm agents]. J Rafsanjan Univ Med Sci. 2013; 12(9): 747-68. [Article in Persian]
7. Wei Q, Ma LZ. Biofilm matrix and its regulation in Pseudomonas aeruginosa. Int J Mol Sci. 2013 Oct; 14(10): 20983-1005. doi:10.3390/ijms141020983
8. Ruhal R, Antti H, Rzhepishevska O, Boulanger N, Barbero DR, Wai SN, et al. A multivariate approach to correlate bacterial surface properties to biofilm formation by lipopolysaccharide mutants of Pseudomonas aeruginosa. Colloids Surf B Biointerfaces. 2015 Mar; 127: 182-91. doi:10.1016/j.colsurfb.2015.01.030
9. Masák J, Čejková A, Schreiberová O, Rezanka T. Pseudomonas biofilms: possibilities of their control. FEMS Microbiol Ecol. 2014 Jul; 89(1): 1-14. doi:10.1111/1574-6941.12344
10. Pratiwi SUT, Lagendijk EL, Hertiani T, Weert Sd, Hondel CAMJJVD. Antimicrobial effects of indonesian medicinal plants extracts on planktonic and biofilm growth of Pseudomonas aeruginosa and Staphylococcus aureus. J Horticulture. 2015; 2: 119. doi:10.4172/2376-0354.1000119
11. Ulloa-Urizar G, Aguilar-Luis MA, De Lama-Odría MD, Camarena-Lizarzaburu J, del Valle Mendoza J. Antibacterial activity of five Peruvian medicinal plants against Pseudomonas aeruginosa. Asian Pac J Trop Biomed. 2015; 5(11): 928-31. https://doi.org/10.1016/j.apjtb.2015.07.016
12. Zargari F, Ghorbanihaghjo A, Babaei H, Farajnia S, Hayati Roodbari N. [The effect of hydroalcoholic extract of Nasturtium officinale R.Br on antioxidant status and DNA damage in liver and kidney rats exposed to arsenic]. Med J Tabriz Univ Med Sci. 2014; 3(36): 44-51. [Article in Persian]
13. Mohammadi J, Safari F, Rad P, Delaviz H. [The effect of Hydroalcoholic extract of Nasturtium officinalis on ovarian hormonal and histological changes after toxicity by doxorubicin in rats]. J RafsanjanUniv Med Sci. 2016; 14(11): 953-64. [Article in Persian]
14. Nikan J, Khavari H. In vitro anti-fungal activity of watercress (Nasturtium officinale) extract against Fusarium solani, the causal agent of potato dry rot. Journal of Herbal Drugs. 2014; 5(1): 19-24.
15. Penecilla GL, Magno CP. Antibacterial activity of extracts of twelve common medicinal plants from the Philippines. J Med Plants Res. 2011; 5(16): 3975-81.
16. Omidi A, Sharifi A. [The effect of methanolic extracts of plants Quercus brantii, Pistacia atlantica and Elaeagnus angustifolia on Biofilm formation of Pseudomonas aeruginosa]. Armaghan-e-Danesh. 2017; 21(10): 999-1012. [Article in Persian]
17. Bokaeian M, Farazmand R, Kyghobadi S, Saeidi S. [The antimicrobial activity of extract of Allium Sativum against Staphylococcus aureus resistant antibacterial]. Journal of Plant Researches (Iranian Journal of Biology). 2015; 28(1): 34-41. [Article in Persian]
18. Akbarian J, Khomeiri M, Sadeghi Mahoonak A, Mahmoodi E. [Antimicrobial effect of extracts Phoenix Dactylifera against pathogenic bacteria and spoilage molds]. Electronic Journal of Food Processing and Preservation. 2013; 5(1): 1-12. [Article in Persian]
19. Ahmady-Asbchin S, Nasrolahi Omran A, Jafari N, Mostafapour MJ, Kia M. [Antibacterial effects of Lavandula Stoechas Essential oil, on gram positive and negative bacteria in vitro]. Med Lab J. 2012; 6(2); 35-41. [Article in Persian]
20. Habibipour R, Moradi Haghgou L. [Study on hydro-alcoholic extract effect of pomegranate peel on pseudomonas aeruginosa biofilm formation]. Avicenna J Clin Med. 2015; 22(3): 195-202. [Article in Persian]
21. Freitas E, Aires A, Rosa EA, Saavedra MJ. Antibacterial activity and synergistic effect between watercress extracts, 2-phenylethyl isothiocyanate and antibiotics against 11 isolates of Escherichia coli from clinical and animal source. Lett Appl Microbiol. 2013; 57(4): 266-273.
22. Abdul DA, Majeed S.N, Ameen B.H. Antioxidant activity, total phenolic content and antimicrobial activity of two medicinal plants from Sulaimani City, Iraqi Kurdistan Region. Advances in Life Science and Technology. 2014; 18: 65-71.
Send email to the article author


XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Omidi A, Sharifi A. Effect of methanolic extract of Nasturtium officinale on growth and biofilm formation of Pseudomonas aeruginosa. J Gorgan Univ Med Sci 2018; 20 (2) :102-108
URL: http://goums.ac.ir/journal/article-1-3369-en.html


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 20, Issue 2 (7-2018) Back to browse issues page
مجله دانشگاه علوم پزشکی گرگان Journal of Gorgan University of Medical Sciences
Persian site map - English site map - Created in 0.04 seconds with 36 queries by YEKTAWEB 4645