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:: Volume 20, Issue 2 (7-2018) ::
J Gorgan Univ Med Sci 2018, 20(2): 109-115 Back to browse issues page
Antibiotic resistance of Salmonella enterica producing Extended-spectrum B-lactamases (ESBLs) type CMY-2, in poultry
Maryam Naderi Mozajin1 , Pejvak Khaki * 2, Fatemeh Noorbakhsh3
1- M.Sc in Cellular and Molecular Biology - Microbiology, Department of Microbiology, Varamin-Pishva Branch, Islamic Azad Univercity, Varamin-Pishva, Iran
2- Associate Professor, Department of Microbiology, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran. p.khaki@rvsri.ac.ir , p.khaki@rvsri.ac.ir
3- Assistant Professor, Department of Microbiology, Varamin-Pishva Branch, Islamic Azad Univercity, Varamin-Pishva, Iran
Abstract:   (11628 Views)
Background and Objective: Salmonella is one of the most important zoonotic pathogens responsible for food-borne infections all over the world. Poultry products are widely acknowledged to be a significant reservoir for Salmonella. This study was done to evaluate the antibiotic resistant of Salmonella enterica producer of beta lactamase spectrum in poultry.
Methods: In this descriptive – laboratort study 70 Salmonella enterica serotypes were collected from poultry. All Salmonella isolates were tested to antimicrobial susceptibility testing by the Kirby-Bauer disk diffusion according to Clinical Laboratory Standards Institute (CLSI). Twenty-nine antibiotics were used in this study. Klebsiella pneumoniae; ATCC 700603 was used as quality control strains. The isolates were determined to be ESBL-producing Salmonella by the conventional double-disk synergy and genotypic method.
Results: Among 70 salmonella isolates, the most prevalent serotypes were S.typhimurium and S.enteritidis. All serotypes were susceptible to gentamicin, ciprofloxacin, oflaxacin, imipenem, enrofloxacin. The common resistance was observed to cephalexin (96%), cefazolin (96%) and cephalotin (65%). Among the 70 Salmonella isolates studied, multi-drug resistance was observed in 59 (84%) isolates. Forty-seven (67%) isolates were found to be ESBL-producing isolates. PCR assay of all isolates showed that 17 isolates (33.3%) carried bala CMY2 gene.
Conclusion: This study showed that antibiotic resistance to Salmonella enterica serotypes is due to beta lactamase enzyme in this strain is considerably increased in poultry.
Keywords: Salmonella enterica, ESBL, Multi-drug resistant, Bala CMY2 gene
Full-Text [PDF 325 kb] [English Abstract]   (15576 Downloads) |   |   Abstract (HTML)  (361 Views)  
Type of Study: Original Articles | Subject: Microbiology
* Corresponding Author Address: Associate Professor, Department of Microbiology, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran. p.khaki@rvsri.ac.ir
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Naderi Mozajin M, Khaki P, Noorbakhsh F. Antibiotic resistance of Salmonella enterica producing Extended-spectrum B-lactamases (ESBLs) type CMY-2, in poultry. J Gorgan Univ Med Sci 2018; 20 (2) :109-115
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Volume 20, Issue 2 (7-2018) Back to browse issues page
مجله دانشگاه علوم پزشکی گرگان Journal of Gorgan University of Medical Sciences
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