[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Home::
Journal Information::
Indexing Sources::
Editorial Board::
Executive Members::
Articles Archive::
Instruction to Authors::
Peer-Review::
Contact Us::
Site Facilities::
::
Search in website

Advanced Search
Receive site information
Enter your Email in the following box to receive the site news and information.
:: Volume 21, Issue 2 (7-2019) ::
J Gorgan Univ Med Sci 2019, 21(2): 50-58 Back to browse issues page
Effect of hydroalcoholic extract of Cannabis sativa on cell survival and differentiation of mesenchymal stem cells derived from human adipose tissue to osteoblast-like cells
Mehregan Jamshidi1 , Seyed Ebrahim Hosseini * 2, Davood Mehrabani3 , Masoud Amini4
1- Ph.D Candidate in Physiology, Department of Biology, College of Science, Fars Science and Research Branch, Islamic Azad University, Fars, Iran. Ph.D Candidate in Physiology, Department of Biology, College of Science, Shiraz Branch, Islamic Azad University, Shiraz, Iran
2- Associate Professor, Department of Biology, College of Science, Shiraz Branch, Islamic Azad University, Shiraz, Iran , ebrahim.hossini@yahoo.com
3- Assistant Professor, Stem Cell Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
4- Assistant Professor, Laparascopy Research Center, Department of Surgery, Shiraz University of Medical Sciences, Shiraz, Iran
Abstract:   (6605 Views)
Background and Objective: The resin secretions of Cannabis sativa are called Hashish, which has medicinal and psychological properties. The most important psychoactive compound of this plant is THC (Delta-9-Tetrahydrocannabinol), which can stimulate cannabinoid receptors in the body. This study was carried out to evaluate the effect of hydroalcoholic extract of Cannabis sativa on cell survival and osteoblastic differentiation of human mesenchymal stem cells.

Methods: In this experimental study, mesenchymal stem cells derived from fat tissue of human abdominal were treated with 100 ng/ml concentration of hydroalcoholic extract of Cannabis sativa. Flow cytometry and RT-PCR techniques were used for detection of cells. The cytotoxic effect of Cannabis sativa extract and osteoblastic differentiation of cells were investigated using MTT method and Alizarin-Red staining, respectively. The karyotype analysis was performed with the preparation of extended metaphase chromosomes.

Results: The identity of the fat mesenchymal stem cells was confirmed by the expression of non-hematopoietic mesenchymal markers (CD90, CD44 and CD73) and the lack of expression of the hematopoietic marker (CD34 and CD45). The Alizarin-Red showed that the treatment with Cannabis sativa has no effect on the osteoblastic differentiation of human fat mesenchymal stem cells, and the treated cells were differentiated into bone cells same as control group. Also, Cannabis sativa extract has no effect on the structure, morphological status and number of chromosomes of these cells.

Conclusion: This study showed that human fat mesenchymal cells in the presence of a hydroalcoholic extract of Cannabis sativa maintain the ability of osteoblastic differentiation. Also, this extract has no effect on the chromosomal karyotype of the cells.
Keywords: Cannabis sativa, Fat mesenchymal cells, Osteoblast, Karyotype
Full-Text [PDF 338 kb]   (12080 Downloads)    
Type of Study: Original Articles | Subject: Stem Cells
References
1. Tettey J. Recommended Methods for the Identification and Analysis of Cannabis and Cannabis Products. New York: United Nations Office on Drugs and Crime Vienna United Nations. 2009.
2. Izzo AA, Borrelli F, Capasso R, Di Marzo V, Mechoulam R. Non-psychotropic plant cannabinoids: new therapeutic opportunities from an ancient herb. Trends Pharmacol Sci. 2009 Oct; 30(10): 515-27. doi: 10.1016/j.tips.2009.07.006
3. Hartman RL, Brown TL, Milavetz G, Spurgin A, Gorelick DA, Gaffney G, et al. Controlled vaporized cannabis, with and without alcohol: subjective effects and oral fluid-blood cannabinoid relationships. Drug Test Anal. 2016 Jul; 8(7): 690-701. doi: 10.1002/dta.1839
4. Borgelt LM, Franson KL, Nussbaum AM, Wang GS. The pharmacologic and clinical effects of medical cannabis. Pharmacotherapy. 2013 Feb; 33(2): 195-209. doi: 10.1002/phar.1187
5. D'Souza DC, Perry E, MacDougall L, Ammerman Y, Cooper T, Wu YT, et al. The psychotomimetic effects of intravenous delta-9-tetrahydrocannabinol in healthy individuals: implications for psychosis. Neuropsychopharmacology. 2004 Aug; 29(8): 1558-72. doi: 10.1038/sj.npp.1300496
6. Kochman LJ, dos Santos AA, Fornal CA, Jacobs BL. Despite strong behavioral disruption, Delta9-tetrahydrocannabinol does not affect cell proliferation in the adult mouse dentate gyrus. Brain Res. 2006 Oct; 1113(1):86-93. doi: 10.1016/j.brainres.2006.07.080
7. Hernán Pérez de la Ossa D, Lorente M, Gil-Alegre ME, Torres S, García-Taboada E, Aberturas Mdel R, et al. Local delivery of cannabinoid-loaded microparticles inhibits tumor growth in a murine xenograft model of glioblastoma multiforme. PLoS One. 2013; 8(1): e54795. doi: 10.1371/journal.pone.0054795
8. Aviello G, Romano B, Borrelli F, Capasso R, Gallo L, Piscitelli F, et al. Chemopreventive effect of the non-psychotropic phytocannabinoid cannabidiol on experimental colon cancer. J Mol Med (Berl). 2012 Aug; 90(8): 925-34. doi: 10.1007/s00109-011-0856-x
9. Fisher T, Golan H, Schiby G, PriChen S, Smoum R, Moshe I, et al. In vitro and in vivo efficacy of non-psychoactive cannabidiol in neuroblastoma. Curr Oncol. 2016 Mar; 23(2): S15-22. doi: 10.3747/co.23.2893
10. Xie J, Xiao D, Xu Y, Zhao J, Jiang L, Hu X, et al. Up-regulation of immunomodulatory effects of mouse bone-marrow derived mesenchymal stem cells by tetrahydrocannabinol pre-treatment involving cannabinoid receptor CB2. Oncotarget. 2016 Feb; 7(6): 6436-47. doi: 10.18632/oncotarget.7042
11. Cakici C, Buyrukcu B, Duruksu G, Haliloglu AH, Aksoy A, Isık A, et al. Recovery of fertility in azoospermia rats after injection of adipose-tissue derived mesenchymal stem cells: The sperm generation. BioMed Research International. Volume 2013, Article ID 529589. http://dx.doi.org/10.1155/2013/529589
12. Bluguermann C,Wu L, Petrigliano F, Mcallister D, Miriuka S, Evseenko DA. Novel aspects of parenchymal–mesenchymal interactions: from cell types to molecules and beyond. Cell Biochemistry and Function. 2013 Jun; 31(4): 271-80. doi: 10.1002/cbf.2950
13. Mehrabani D, Hassanshahi MA, Tamadon A, Zare S, Keshavarz S, Rahmanifar F, et al. Adipose tissue-derived mesenchymal stem cells repair germinal cells of seminiferous tubules of busulfan-induced azoospermic rats. J Hum Reprod Sci. 2015 Apr-Jun; 8(2): 103-10. doi: 10.4103/0974-1208.158618
14. Di Battista JA, Shebaby W, Kizilay O, Hamade E, Abou Merhi R, Mebarek S, et al. Proliferation and differentiation of human adipose-derived mesenchymal stem cells (ASCs) into osteoblastic lineage are passage dependent. Inflamm Res. 2014 Nov; 63(11): 907-17. doi: 10.1007/s00011-014-0764-y
15. Al-Nbaheen M, Vishnubalaji R, Ali D, Bouslimi A, Al-Jassir F, Megges M, et al. Human stromal (mesenchymal) stem cells from bone marrow, adipose tissue and skin exhibit differences in molecular phenotype and differentiation potential. Stem Cell Rev. 2013 Feb; 9(1): 32-43. doi: 10.1007/s12015-012-9365-8
16. Abdanipour A, Noori-Zadeh A, Mohamadi Z, Rashid Sheykh Ahmad F, Akbari P. [Comparison of adipose-derived stem cells and bone marrow stromal cells in prolonged passages based on viability and auto-differentiation]. J Rafsanjan Univ Med Sci. 2015; 13(12): 1141-52. [Article in Persian]
17. Solali S, Kaviani S, Soleimani M, Zonoubi Z. [Isolation and characterization of mesenchymal stem cells derived from adipose tissue]. Koomesh. 2015; 16(4): 505-11. [Article in Persian]
18. de Lago E, Gómez-Ruiz M, Moreno-Martet M, Fernández-Ruiz J. Cannabinoids, multiple sclerosis and neuroprotection. Expert Rev Clin Pharmacol. 2009 Nov; 2(6): 645-60. doi: 10.1586/ecp.09.42
19. Rieder SA, Chauhan A, Singh U, Nagarkatti M, Nagarkatti P. Cannabinoid-induced apoptosis in immune cells as a pathway to immunosuppression. Immunobiology. 2010; 215(8): 598-605. doi: 10.1016/j.imbio.2009.04.001
20. Chakravarti B, Ravi J, Ganju RK. Cannabinoids as therapeutic agents in cancer: current status and future implications. Oncotarget. 2014 Aug; 5(15): 5852-72. 10.18632/oncotarget.2233
21. Bamdadpasand Shekarsarayi F, Eslami Farsani M, Heydariya N. [The effect of soy isoflavone on the proliferation and differentiation of adipose-derived mesenchymal stem cells into chondrocytes and expression of collagen II and aggrecan genes]. Qom Univ Med Sci J. 2017; 11(1): 18-28. [Article in Persian]
22. Milman G, Bergamaschi MM, Lee D, Mendu DR, Barnes AJ, Vandrey R, et al. Plasma cannabinoid concentrations during dronabinol pharmacotherapy for cannabis dependence. Ther Drug Monit. 2014 Apr; 36(2): 218-24. doi: 10.1097/FTD.0b013e3182a5c446
23. Berghaus G, Scheer N, Schmidt P. Effects of Cannabis on Psychomotor Skills and Driving Performance - a Metaanalysis of Experimental Studies. 13th International Conference on Alcohol, Drugs and Traffic Safety (T'95). Australia, 13-18 August 1995.
24. Appendino G, Chianese G, Taglialatela-Scafati O. Cannabinoids: occurrence and medicinal chemistry. Curr Med Chem. 2011; 18(7): 1085-99.
25. Khare M, Taylor AH, Konje JC, Bell SC. Delta9-tetrahydrocannabinol inhibits cytotrophoblast cell proliferation and modulates gene transcription. Mol Hum Reprod. 2006 May; 12(5): 321-33. doi: 10.1093/molehr/gal036
26. Galve-Roperh I, Chiurchiù V, Díaz-Alonso J, Bari M, Guzmán M, Maccarrone M. Cannabinoid receptor signaling in progenitor/stem cell proliferation and differentiation. Prog Lipid Res. 2013 Oct; 52(4): 633-50. doi: 10.1016/j.plipres.2013.05.004
27. Gowran A, McKayed K, Campbell VA. The cannabinoid receptor type 1 is essential for mesenchymal stem cell survival and differentiation: implications for bone health. Stem Cells Int. 2013; 2013: 796715. doi: 10.1155/2013/796715
28. Prenderville JA, Kelly ÁM, Downer EJ. The role of cannabinoids in adult neurogenesis. Br J Pharmacol. 2015 Aug; 172(16): 3950-63. doi: 10.1111/bph.13186
29. Maccarrone M, Guzmán M, Mackie K, Doherty P, Harkany T. Programming of neural cells by (endo) cannabinoids: from physiological rules to emerging therapies. Nat Rev Neurosci. 2014 Dec; 15(12): 786-801. doi: 10.1038/nrn3846
30. Karsenty G. Convergence between bone and energy homeostases: leptin regulation of bone mass. Cell Metab. 2006 Nov; 4(5): 341-48. doi: 10.1016/j.cmet.2006.10.008
31. Jamshidi M, Hosseini SE, Mehrabani D, Amini M. Effect of hydroalcoholic extract of Cannabis (Cannabis sativa L.) on morphology and the process of human adipose-drived mesenchymal stem cell growth. Electron J Gen Med. 2018; 15(3): em31. doi:10.29333/ejgm/86194
Send email to the article author


XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Jamshidi M, Hosseini S E, Mehrabani D, Amini M. Effect of hydroalcoholic extract of Cannabis sativa on cell survival and differentiation of mesenchymal stem cells derived from human adipose tissue to osteoblast-like cells. J Gorgan Univ Med Sci 2019; 21 (2) :50-58
URL: http://goums.ac.ir/journal/article-1-3298-en.html


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 21, Issue 2 (7-2019) Back to browse issues page
مجله دانشگاه علوم پزشکی گرگان Journal of Gorgan University of Medical Sciences
Persian site map - English site map - Created in 0.04 seconds with 36 queries by YEKTAWEB 4657