[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Home::
Journal Information::
Indexing Sources::
Editorial Board::
Executive Members::
Articles Archive::
Instruction to Authors::
Peer-Review::
Contact Us::
Site Facilities::
::
Search in website

Advanced Search
Receive site information
Enter your Email in the following box to receive the site news and information.
:: Volume 21, Issue 2 (7-2019) ::
J Gorgan Univ Med Sci 2019, 21(2): 9-17 Back to browse issues page
Effect of combined Atorvastatin and zinc oxide on the biochemical and histopathological alterations in kidney of diabetic rats
Zahra Karampour Gebchag1 , Reza Heidari * 2, Seyyed Meysam Abtahi-Froushani3 , Farah Farokhi4
1- M.Sc in Biochemistry, Department of Biology, Faculty of Science, Urmia University, Urmia, Iran
2- Professor, Department of Biology, Faculty of Science, Urmia University, Urmia, Iran , r.heidari@urmia.ac.ir
3- Assistant Professor, Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
4- Associate Professor, Department of Biology, Faculty of Science, Urmia University, Urmia, Iran
Abstract:   (5532 Views)

Background and Objective: Diabetic mellitus nephropathy is one of the most important implication factors in kidney´s physiological function in diabetes mellitus. Having major role in filtration, in hyperglycemic condition kidney has shown more damages in comparison with other tissues. This study was done to determine the effect of combined Atorvastatin and Zinc oxide on the biochemical and histopathological alterations in kidney of diabetic rats.

Methods: In this experimental study, 40 female Wistar rats were randomly allocated into five groups including normal control (NC), diabetic control (DC), diabetic rats treated with atorvastatin (20mg/kg/bw daily, orally) (D+A), Zinc oxide (30mg/kg/bw daily, orally) (D+Z) and combination of each drug in half dose (daily, orally) (D+A+Z). Diabetes induced in rats by a single intraperitoneal injection of 60mg/kg/bw streptozotocin-diabetic.Animals treated for one month. At the end of the study, kidney weight and body weight and biochemical factors including creatinine and urea were measured to assess renal function. For determing the histopathology of kidney tissue, sections with 4-5 micrometer were stained with hematoxylin and eosin.

Results: The level of serum creatinine and urea was significantly increased in diabetic rats in compare to controls (P<0.05). Treatment of diabetic rats with half doses of combination of atorvastatin and Zinc oxide reduced the level of creatinine, urea and renal tissue damage in comparision with diabetic rats without treatment (P<0.05).

Conclusion: This study showed that the combination of atorvastatin and Zinc oxide has effect on controlling diabetic nephropathy.

Keywords: Diabetes mellitus, Diabetic nephropathy, Atorvastatin, Zinc oxide, Rat
Full-Text [PDF 474 kb]   (14294 Downloads)    
Type of Study: Original Articles | Subject: Physiology - Pharmacology
References
1. Navarro-González JF, Mora-Fernández C, Muros de Fuentes M, García-Pérez J. Inflammatory molecules and pathways in the pathogenesis of diabetic nephropathy. Nat Rev Nephrol. 2011 Jun; 7(6): 327-40. doi: 10.1038/nrneph.2011.51
2. Moeller MJ, Tenten V. Renal albumin filtration: alternative models to the standard physical barriers. Nat Rev Nephrol. 2013 May; 9(5): 266-77. doi: 10.1038/nrneph.2013.58
3. Rivero A, Mora C, Muros M, García J, Herrera H, Navarro-González JF. Pathogenic perspectives for the role of inflammation in diabetic nephropathy. Clin Sci (Lond). 2009 Mar; 116(6): 479-92. doi: 10.1042/CS20080394
4. Wada J, Makino H. Inflammation and the pathogenesis of diabetic nephropathy. Clin Sci (Lond). 2013 Feb; 124(3): 139-52. doi: 10.1042/CS20120198
5. Elmarakby AA, Sullivan JC. Relationship between oxidative stress and inflammatory cytokines in diabetic nephropathy. Cardiovasc Ther. 2012 Feb; 30(1): 49-59. doi: 10.1111/j.1755-5922.2010.00218.x
6. Murrow JR, Sher S, Ali S, Uphoff I, Patel R, Porkert M, et al. The differential effect of statins on oxidative stress and endothelial function: atorvastatin versus pravastatin. J Clin Lipidol. 2012 Jan-Feb; 6(1): 42-49. doi: 10.1016/j.jacl.2011.08.006
7. Forbes JM, Coughlan MT, Cooper ME. Oxidative stress as a major culprit in kidney disease in diabetes. Diabetes. 2008 Jun; 57(6): 1446-54. doi: 10.2337/db08-0057
8. Afshari AT, Shirpoor A, Farshid A, Saadatian R, Rasmi Y, Saboory E, et al. The effect of ginger on diabetic nephropathy, plasma antioxidant capacity and lipid peroxidation in rats. Food Chemistry. 2007; 101(1): 148-53. https://doi.org/10.1016/j.foodchem.2006.01.013
9. Chen X, Hu X, Zou Y, Pi R, Liu M, Wang T, et al. Combined treatment with minocycline and prednisone attenuates experimental autoimmune encephalomyelitis in C57 BL/6 mice. J Neuroimmunol. 2009 May; 210(1-2): 22-29. doi: 10.1016/j.jneuroim.2009.02.016
10. Conway D, Cohen JA. Combination therapy in multiple sclerosis. Lancet Neurol. 2010 Mar; 9(3): 299-308. doi: 10.1016/S1474-4422(10)70007-7
11. Luo JD, Zhang WW, Zhang GP, Zhong BH, Ou HJ. Effects of simvastatin on activities of endogenous antioxidant enzymes and angiotensin-converting enzyme in rat myocardium with pressure-overload cardiac hypertrophy. Acta Pharmacol Sin. 2002 Feb; 23(2): 124-28.
12. Lefer DJ, Scalia R, Jones SP, Sharp BR, Hoffmeyer MR, Farvid AR, et al. HMG-CoA reductase inhibition protects the diabetic myocardium from ischemia-reperfusion injury. FASEB J. 2001 Jun; 15(8): 1454-56.
13. Kishi T, Hirooka Y, Konno S, Sunagawa K. Atorvastatin improves the impaired baroreflex sensitivity via anti-oxidant effect in the rostral ventrolateral medulla of SHRSP. Clin Exp Hypertens. 2009 Nov; 31(8): 698-704. doi: 10.3109/10641960903407066
14. Mooradian AD, Haas MJ, Batejko O, Hovsepyan M, Feman SS. Statins ameliorate endothelial barrier permeability changes in the cerebral tissue of streptozotocin-induced diabetic rats. Diabetes. 2005 Oct; 54(10): 2977-82.
15. Grip O, Janciauskiene S, Bredberg A. Use of atorvastatin as an anti-inflammatory treatment in Crohn's disease. Br J Pharmacol. 2008 Dec; 155(7): 1085-92. doi: 10.1038/bjp.2008.369
16. Li J, Sun YM, Wang LF, Li ZQ, Pan W, Cao HY. Comparison of effects of simvastatin versus atorvastatin on oxidative stress in patients with coronary heart disease. Clin Cardiol. 2010 Apr; 33(4): 222-27. doi: 10.1002/clc.20724
17. Mohammadi MT, Ramezani Binabaj M, Mirjalili MH, Ghaedniaye Jahromi M, Jafari M, Salem F. [Effect of atorvastatin on pancreatic oxidative stress in Streptozotocin-induced diabetic rat]. Int J Endocrinol Metab. 2013; 15(2): 197-204. [Article in Persian]
18. Mohammadi MT, Amini R, Jahanbakhsh Z, Shekarforoush S. Effects of atorvastatin on the hypertension-induced oxidative stress in the rat brain. Iran Biomed J. 2013; 17(3): 152-57.
19. Furukawa M, Gohda T, Tanimoto M, Tomino Y. Pathogenesis and novel treatment from the mouse model of type 2 diabetic nephropathy. Sci World J. Volume 2013, Article ID: 928197. http://dx.doi.org/10.1155/2013/928197
20. Umrani RD, Paknikar KM. Zinc oxide nanoparticles show antidiabetic activity in streptozotocin-induced Type 1 and 2 diabetic rats. Nanomedicine (Lond). 2014 Jan; 9(1): 89-104. doi: 10.2217/nnm.12.205
21. Barrett KE, Barman SM, Boitano S, Brooks HL. Cardiovascular regulatory mechanisms. Ganong's Review of Medical Physiology. 23rd edition. New Delhi: Tata McGraw-Hill Companies. 2010; pp: 555-68.
22. Eller P, Eller K, Wolf AM, Reinstadler SJ, Tagwerker A, Patsch JR, et al. Atorvastatin attenuates murine anti-glomerular basement membrane glomerulonephritis. Kidney Int. 2010 Mar; 77(5): 428-35. doi: 10.1038/ki.2009.478
23. Sheikhpour R. [Evaluation of the effect of Zinc supplement on serum lipids level in type II diabetic patients]. J Ardabil Univ Med Sci. 2011; 11(1): 59-66. [Article in Persian]
24. Sancheti S, Sancheti S, Bafna M, Seo SY. Antihyperglycemic, antihyperlipidemic, and antioxidant effects of Chaenomeles sinensis fruit extract in streptozotocin-induced diabetic rats. Eur Food Res Technol. 2010; 231(3): 415-21.
25. Hosseinzadeh H, Ramezani M, Danaei AR. Antihyperglycaemic effect and acute toxicity of Securigera Securidaca L. seed extracts in mice. Phytother Res. 2002 Dec; 16(8): 745-47. doi: 10.1002/ptr.1020
26. Shokrzadeh M, Jahani M, Vafaeipour Z, Shaki F. [Protective effect of Nanoceria against renal mitochondrial damage in Streptozocine-induced diabetic mice]. J Mazandaran Univ Med Sci. 2016; 25 (132): 258-69. [Article in Persian]
27. Perkins BA, Ficociello LH, Roshan B, Warram JH, Krolewski AS. In patients with type 1 diabetes and new-onset microalbuminuria the development of advanced chronic kidney disease may not require progression to proteinuria. Kidney Int. 2010 Jan; 77(1): 57-64. doi: 10.1038/ki.2009.399
28. Satchell SC, Tooke JE. What is the mechanism of microalbuminuria in diabetes: a role for the glomerular endothelium? Diabetologia. 2008; 51(5): 714-25. doi: 10.1007/s00125-008-0961-8
29. Bwititi P, Musabayane CT, Nhachi CF. Effects of Opuntia megacantha on blood glucose and kidney function in streptozotocin diabetic rats. J Ethnopharmacol. 2000 Mar; 69(3): 247-52.
30. Rajasekaran S, Sivagnanam K, Subramanian S. Mineral contents of aloe vera leaf gel and their role on streptozotocin-induced diabetic rats. Biol Trace Elem Res. 2005; 108 (1-3): 185-95. doi: 10.1385/BTER:108:1-3:185
31. Shokeen P, Anand P, Murali YK, Tandon V. Antidiabetic activity of 50% ethanolic extract of Ricinus communis and its purified fractions. Food Chem Toxicol. 2008 Nov; 46(11): 3458-66. doi: 10.1016/j.fct.2008.08.020
32. Al-Attar AM, Zari TA. Influences of crude extract of tea leaves, Camellia sinensis, on streptozotocin diabetic male albino mice. Saudi J Biol Sci. 2010 Oct; 17(4): 295-301. doi: 10.1016/j.sjbs.2010.05.007
33. Flyvbjerg A, Bornfeldt KE, Orskov H, Arnqvist HJ. Effect of insulin-like growth factor I infusion on renal hypertrophy in experimental diabetes mellitus in rats. Diabetologia. 1991 Oct; 34(10): 715-20.
34. Bilous R. Renal structural damage in IDDM and NIDDM functional relationships. London: John Wiley & Sons. 2001; pp: 71-89.
35. Kolset SO, Reinholt FP, Jenssen T. Diabetic nephropathy and extracellular matrix. J Histochem Cytochem. 2012 Dec; 60(12): 976-86. doi: 10.1369/0022155412465073
36. Kotajima N, Kimura T, Kanda T, Obata K, Kuwabara A, Fukumura Y, Kobayashi I. Type IV collagen as an early marker for diabetic nephropathy in non-insulin-dependent diabetes mellitus. J Diabetes Complications. 2000 Jan-Feb; 14(1): 13-7.
37. Sottile J.Regulation of angiogenesis by extracellular matrix. Biochim Biophys Acta. 2004 Mar; 1654(1): 13-22. doi: 10.1016/j.bbcan.2003.07.002
Send email to the article author


XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Karampour Gebchag Z, Heidari R, Abtahi-Froushani S M, Farokhi F. Effect of combined Atorvastatin and zinc oxide on the biochemical and histopathological alterations in kidney of diabetic rats. J Gorgan Univ Med Sci 2019; 21 (2) :9-17
URL: http://goums.ac.ir/journal/article-1-3245-en.html


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 21, Issue 2 (7-2019) Back to browse issues page
مجله دانشگاه علوم پزشکی گرگان Journal of Gorgan University of Medical Sciences
Persian site map - English site map - Created in 0.04 seconds with 36 queries by YEKTAWEB 4657