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:: Volume 19, Issue 2 (7-2017) ::
J Gorgan Univ Med Sci 2017, 19(2): 84-90 Back to browse issues page
Molecular evaluation of vanA and vanB genes of enterococci isolates resistant to Vancomycin and Teicoplanin
Sh Shahraki1 , M Rabi Nezhad Mousavi * 2, B Dahmarde3 , M Atashgah4
1- Assistant Professor, Department of Microbiology, Infectious Disease and Tropical Medicine Research Center, Faculty of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran
2- M.Sc in Microbiology, Department of Microbiology, Infectious Disease and Tropical Medicine Research Center, Faculty of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran , mortzamoosavy7@gmail.com
3- B.Sc in Microbiology, Department of Microbiology, Faculty of Medicine, Zahedan University of Medical Science, Zahedan, Iran
4- B.Sc in Biology, Department of Microbiology, Faculty of Medicine, Zahedan University of Medical Science, Zahedan, Iran
Abstract:   (10539 Views)

Background and Objective: Enterococci is gram positive bacteria which is the inhabitants of gastrointestinal tract. Hospital infections and antibiotic resistance to enterococci is increased. This study was done to determine the molecular evaluation of vanA and vanB genes of enterococci isolates resistant to Vancomycin and Teicoplanin.

Methods: In this descriptive study, 113 isolates samples were collected and identified according to biochemical test and cultural characteristics in Ali ibn Abi Talib hospital in Zahedan, Iran. Antibiogram test was done to determine antibiotic resistance pattern. E-test strip was used to evaluate the minimum inhibitory of concentration (MIC). PCR was used to detect the vanA and vanB genotype in Vancomycin and Teicoplanin resistance enterococci.

Results: 92%, 6.2% and 1.8% of isolated samles collocted from urine, blood culture and pleura fluid, respectively. According to phenotype, 18.6% and 17.69% were resistance to Vancomycin and Teicoplanin, respectively. Resistance was observed in strains of Enterococcus faecalis and Enterococcus faecium. VanA genotype was seen in all of the resistance isolated species.

Conclusion: This study showed that strains of Enterococcus faecalis and Enterococcus faecium have more antibiotic resistance to the Vancomycin and Teicoplanin, morever vanA genotype precence in all of resistance isolated samples.

Keywords: Enterococci, Vancomycin, Teicoplanin, Antibiotic resistance, VanA genotype, VanB genotype
Full-Text [PDF 375 kb] [English Abstract]   (13544 Downloads) |   |   Abstract (HTML)  (1173 Views)  
Type of Study: Original Articles | Subject: Microbiology
References
1. Hammad AM, Shimamoto T, Shimamoto T. Genetic characterization of antibiotic resistance and virulence factors in Enterococcus spp. from Japanese retail ready-to-eat raw fish. Food Microbiol. 2014 Apr; 38: 62-6. doi:10.1016/j.fm.2013.08.010
2. Sharifi Y, Hasani A, Ghotaslou R, Varshochi M, Hasani A, Aghazadeh M, et al. Survey of Virulence Determinants among Vancomycin Resistant Enterococcus faecalis and Enterococcus faecium Isolated from Clinical Specimens of Hospitalized Patients of North west of Iran. Open Microbiol J. 2012; 6:34-9. doi:10.2174/1874285801206010034
3. Sun H, Wang H, Xu Y, Jones RN, Costello AJ, Liu Y, et al. Molecular characterization of vancomycin-resistant Enterococcus spp. clinical isolates recovered from hospitalized patients among several medical institutions in China. Diagn Microbiol Infect Dis. 2012 Dec; 74(4): 399-403. doi:10.1016/j.diagmicrobio.2012.09.006
4. Praharaj I, Sujatha S, Parija SC. Phenotypic & genotypic characterization of vancomycin resistant Enterococcus isolates from clinical specimens. Indian J Med Res. 2013 Oct; 138(4): 549-56.
5. Aslam M, Diarra MS, Checkley S, Bohaychuk V, Masson L. Characterization of antimicrobial resistance and virulence genes in Enterococcus spp. isolated from retail meats in Alberta, Canada. Int J Food Microbiol. 2012 Jun; 156(3): 222-30. doi:10.1016/j.ijfoodmicro.2012.03.026
6. Javadi AB, Ataei B, Khorvash F, Toghyani S, Mobasherzadeh S, Soghrati M. Prevalence of Vancomycin Resistant Enterococci colonization in gastrointestinal tract of hospitalized patients. Iran J of Clin Infect Dis. 2008; 3(3): 137-41.
7. Hasibi M, Rezaei J, Mohajer Iravani B, Moslemi B, Rahimi HM, Taghavi SM, et al. Hospital-Acquired Vancomycin-Resistant Enterococci. A Report of 2-year Experience. Acta Medica Iranica. 2009; 47(6): 469-72.
8. Al-Tonbary YA, Soliman OE, Sarhan MM, Hegazi MA, El-Ashry RA, El-Sharkawy AA, et al. Nosocomial infections and fever of unknown origin in pediatric hematology/oncology unit: a retrospective annual study. World J Pediatr. 2011 Feb; 7(1): 60-4. doi:10.1007/s12519-010-0212-1
9. Gurtler V, Grando D, Mayall BC, Wang J, Ghaly-Derias S. A novel method for simultaneous Enterococcus species identification/typing and van genotyping by high resolution melt analysis. J Microbiol Methods. 2012 Sep; 90(3): 167-81. doi:10.1016/j.mimet.2012.05.002
10. de Moura TM, Vaz Cassenego AP, Campos FS, Leal Ribeiro AM, Franco AC, d'Azevedo PA, et al. Detection of vanC1 gene transcription in vancomycin-susceptible Enterococcus faecalis. Mem Inst Oswaldo Cruz. 2013 Jun; 108(4): 453-56. doi:10.1590/0074-0276108042013009
11. Xu X, Lin D, Yan G, Ye X, Wu S, Guo Y, et al. vanM, a new glycopeptide resistance gene cluster found in Enterococcus faecium. Antimicrob Agents Chemother. 2010 Nov; 54(11): 4643-7. doi:10.1128/AAC.01710-09
12. Forbes BA SD, Weissfeld A. Bailey & Scott's Diagnostic Microbiology. Virginia: Elsevier Health Sciences. 11st ed. 2002; pp: 236-40.
13. National Nosocomial Infections Surveillance System. National Nosocomial Infections Surveillance (NNIS) System Report, data summary from January 1992 through June 2004, issued October 2004. Am J Infect Control. 2004 Dec; 32(8): 470-85.
14. Comerlato CB, Resende MC, Caierão J, d'Azevedo PA. Presence of virulence factors in Enterococcus faecalis and Enterococcus faecium susceptible and resistant to vancomycin. Mem Inst Oswaldo Cruz. 2013 Aug; 108(5): 590-5.
15. Protonotariou E, Dimitroulia E, Pournaras S, Pitiriga V, Sofianou D, Tsakris A. Trends in antimicrobial resistance of clinical isolates of Enterococcus faecalis and Enterococcus faecium in Greece between 2002 and 2007. J Hosp Infect. 2010 Jul; 75(3): 225-7. doi:10.1016/j.jhin.2009.12.007
16. Wang JT, Chang SC, Wang HY, Chen PC, Shiau YR, Lauderdale TL. High rates of multidrug resistance in Enterococcus faecalis and E. faecium isolated from inpatients and outpatients in Taiwan. Diagn Microbiol Infect Dis. 2013 Apr; 75(4): 406-11. doi:10.1016/j.diagmicrobio.2013.01.004
17. Kobayashi N, Alam M, Nishimoto Y, Urasawa S, Uehara N, Watanabe N. Distribution of aminoglycoside resistance genes in recent clinical isolates of Enterococcus faecalis, Enterococcus faecium and Enterococcus avium. Epidemiol Infect. 2001 Apr; 126(2): 197-204.
18. Hoseinizadeh A, Abtahi H, Shojapour M, Akbari M, Nazari R, Sofian M. [Prevalence and antimicrobial susceptibility pattern of vancomycin resistant enterococci isolated from clinical sample of educational hospitals in Arak]. J Arak Univ Med Sci. 2012; 15(6): 11-16. [Article in Persian]
19. Shokoohi Zade, L, Mohabbati Mobarez A, Alebooye M, Ranjbar R, Zali MR. [Antibiotic Resistance Patterns of E.Faecium and E.Faecalis Strains Isolated from ICUs]. Medical Laboratory Journal. 2015; 8(4): 34-41. [Article in Persian]
20. Naghipoor E, Raefi A, NasrollahiOmran A. [Resistance Pattern of Enterococci Isolated from Nosocomial Infections in the Hospitals Located in Gonbad and Gorgan Cities]. Medical Laboratory Journal. 2015: 8(4): 61-5.
21. Biendo M, Adjide C, Castelain S, Belmekki M, Rousseau F, Slama M, et al. Molecular characterization of glycopeptide-resistant enterococci from hospitals of the picardy region (France). Int J Microbiol. 2010; 2010: 150464. doi:10.1155/2010/150464
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Shahraki S, Rabi Nezhad Mousavi M, Dahmarde B, Atashgah M. Molecular evaluation of vanA and vanB genes of enterococci isolates resistant to Vancomycin and Teicoplanin. J Gorgan Univ Med Sci 2017; 19 (2) :84-90
URL: http://goums.ac.ir/journal/article-1-3082-en.html


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Volume 19, Issue 2 (7-2017) Back to browse issues page
مجله دانشگاه علوم پزشکی گرگان Journal of Gorgan University of Medical Sciences
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