[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Home::
Journal Information::
About the Journal
Aims and Scope
Content Coverage
Conflict of Interest
Publication Ethics
Editorial Board::
Executive Members::
Instruction to Authors::
Peer Review::
Articles Archive::
Current Issue
All Issues
Indexing Databases::
Contact Us::
Contact Us
Contact Form
Site Facilities::
Search contents
::
Search in website

Advanced Search
Receive site information
Enter your Email in the following box to receive the site news and information.
:: Volume 19, Issue 2 (7-2017) ::
J Gorgan Univ Med Sci 2017, 19(2): 65-71 Back to browse issues page
Degradation of polycyclic hydrocarbones anthracene by using Pseudomonas aeruginusa
M Sasani1 , Sh Khoramnejadian * 2, R Safari3
1- M.Sc Student in Environmental Pollution, Department of Environment, Damavand Branch, Islamic Azad University, Damavand, Iran
2- Assistant Professor, Department of Environment, Damavand Branch, Islamic Azad University, Damavand, Iran , khoramnejad@damavandiau.ac.ir
3- Academic Instructor, Caspian Sea Ecology Research Institute, Iranian Fisheries Science Research Institute, Agricultural Research Education and Extension Organization, Mazandaran, Iran
Abstract:   (7398 Views)

Background and Objective: Polycyclic Aromatic Hydrocarbons (PAHs) are the most important organic pollutants that causing multiple side effects including carcinogenic, mutagenic and toxicity. Among the aromatic compounds degrading bacteria, pseudomonas produce board spectrum of degrading enzymes and are used, as biological tools, for decreasing of PAHs. This study was done to evaluate the degradation of polycyclic hydrocarbones anthracene by using Pseudomonas aeruginusa.

Methods: In this descriptive – analytic study, sampling was collected from river estuary sediment and had cultured in Minimal Salt Medium (MSM). Pseudomonas aeruginosa was one of the isolated bacteria from river sediment which identified by molecular technique. In next step, influence of pH (6.5 and 7.5) temperature (25 and 35°C) and concentration of anthracene (150 and 200 ppm) were surveyed on anthracene biodegradation and bacterial growth during zero, 24 and 48 hours by HPLC and spectrophotometry method respectively.

Results: The results showed that the optimized condition for biodegradation included pH=7.5, 35°C and 150 ppm of anthracene. Bacterial degradation of anthracene was increased with prolong of incubation time. Biodegradation efficiency of anthracene in the presence of pseudomonas was 50% within 2 days, which indicates the ability of the bacteria for the enzymes production.

Conclusion: High growth potential of pseudomonas in unsuitable areas and due to the production of degrading enzymes, it can be used as indicator bacteria used to remove anthracene.
Keywords: Polycyclic aromatic hydrocarbons, Anthracene, Pseudomonas aeruginosa
Full-Text [PDF 316 kb] [English Abstract]   (15206 Downloads) |   |   Abstract (HTML)  (1244 Views)  
Type of Study: Original Articles | Subject: Environmental Health
References
1. Lease CwM, Bentham RH, Gaskin SE, Juhasz AL. Isolation and identification of pyrene mineralizing Mycobacterium spp. from contaminated and uncontaminated sources. Appl Environ Soil Sci. 2011; 46(7): 171-83. http://dx.doi.org/10.1155/2011/409643
2. Coral G, Karagoz S. Isolation and characterization of phenanthrenene-degrading bacteria from a petroleum refinery soil. Ann Microbiol. 2005; 55(4): 255-9.
3. Boyle D, Wiesner C, Richardson A. Factors affecting the degradation of polycyclic aromatic hydrocarbons in soil by white rot fungi. Soil Biology and Biochemistry. Soil Biology and Biochemistry. 1998 Jul; 30(7): 873-82. http://doi.org/10.1016/S0038-0717(97)00219-8
4. Emtiazjo M, Sedighi S, Emtiazjo M. [Bioremediation of anthracene in siri island with and emphasis on biosfety]. Journal of Environmental Science and Technology. 2009; 11(3): 258-68. [Article in Persian]
5. Water Environment, Federation. Standard Methods for the Examination of Water and Wastewater. 20th ed. Washington DC: American Public Health Association. 1998; pp: 123-32.
6. Yaghobzadeh Z, Safari R. [Naphthalene biodegradation by bacteria isolated from the Caspin sea]. Journal of Marine Siences and Technology. 2006; 5(1-2): 89-96. [Article in Persian]
7. Cerniglia CE. Biodegradation of polycyclic aromatic hydrocarbons. Biodegradation. 1992; 3(2): 351-68. doi:10.1007/BF00129093
8. Samaei MR, Mortazavi SB, Bakhshi B, Jonidi Jafari A. Isolation, genetic identification, and degradation characteristics of N- hexadecane degrading bacteria from tropical areas in Iran. Fresenius Environmental Bulletin. 2013 Jan; 22(4): 1304-12.
9. Barret M, Carrère H, Delgadillo L, Patureau D. PAH fate during the anaerobic digestion of contaminated sludge: Do bioavailability and/or cometabolism limit their biodegradation? Water Res. 2010 Jul; 44(13): 3797-806. doi:10.1016/j.watres.2010.04.031
10. Abou-Arab AAK, Abou-Bakr S, Maher RA, El-Hendawy HH, Awad AA. Degradation of Polycyclic Aromatic Hydrocarbons as Affected by some Lactic Acid Bacteria. J Am Sci. 2010; 10(6): 1237-46.
11. Prakash A, Bisht S, Singh J, Teotia P, Kela R, Kumar V. Biodegradation potential of petroleum hydrocarbons by bacteria and mixed bacterial consortium isolated from contaminated sites. Turkish J Eng Env Sci. 2014; 38: 41-50. doi:10.3906/muh-1306-4
12. Jacques RJS, Santos EC, Bento FM, Peralba MCR, Selbach PA, Sá ELS, et al. Anthracene biodegradation by Pseudomonas sp. isolated from a petrochemical sludge landfarming site. International Biodeterioration & Biodegradation. 2005 Oct; 56(3): 143-50. https://doi.org/10.1016/j.ibiod.2005.06.005
13. Simarro R, González N, Bautista LF, Molina MC. Biodegradation of high-molecular-weight polycyclic aromatic hydrocarbons by a wood-degrading consortium at low temperatures. FEMS Microbiol Ecol. 2013 Feb; 83(2): 438-49. doi:10.1111/1574-6941.12006
14. Cripps C, Bumpus JA, Aust SD. Biodegradation of azo and heterocyclic dyes by Phanerochaete chrysosporium. Appl Environ Microbiol. 1990 Apr; 56(4): 1114-8.
15. Bisht S, Pandey P, Sood A, Sharma Sh, Bisht NS. Biodegradation of naphthalene and anthracene by chemo-tavtically active rhizobacteria of Populus deltoides. Braz J Microbiol. 2010; 41(4): 922-30.
16. Obayori OS, Adebusoye SA, Adewale AO, Oyetibo GO, Oluyemi OO, et al. Differential degradation of crude oil (Bonny Light) by four Pseudomonas strains. J Environ Sci (China). 2009; 21(2): 243-8.
17. Yousefi Z, Safari R, Sarvi S, Mohammadpour Tahmtan RA, Rostamali E. [Anthracene Biodegradation by Bacteria Isolated from Tajan River Estuary]. J Mazandaran Univ Med Sci. 2014; 24 (118) :111-22. [Article in Persian]
18. Cerniglia CE. Microbial metabolism of polycyclic aromatic hydrocarbons. Adv Appl Microbiol. 1984; 30: 31-71.
Send email to the article author

XML   Persian Abstract   Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Sasani M, Khoramnejadian S, Safari R. Degradation of polycyclic hydrocarbones anthracene by using Pseudomonas aeruginusa. J Gorgan Univ Med Sci 2017; 19 (2) :65-71
URL: http://goums.ac.ir/journal/article-1-3079-en.html
Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 19, Issue 2 (7-2017) Back to browse issues page
مجله دانشگاه علوم پزشکی گرگان Journal of Gorgan University of Medical Sciences
Persian site map - English site map - Created in 0.05 seconds with 36 queries by YEKTAWEB 4660
Creative Commons License
This work is licensed under a Creative Commons — Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)