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:: Volume 19, Issue 1 (3-2017) ::
J Gorgan Univ Med Sci 2017, 19(1): 67-71 Back to browse issues page
Compraision of urease test with Giemsa staining for detection of Helicobacter pylori infection in patients with gastric ulcer and gastritis
R Davaloo1 , H Kaboosi2 , Kh Heidari3 , R Azarhoush4 , E Naeimi-Tabiei5 , V Kazeminejad * 6
1- General Physician, Golestan Univercity of Medical Sciences, Gorgan, Iran
2- Assistant Professor, Department of Microbiology, Ayat Ollah Amoli Branch, Islamic Azad University, Amol, Iran
3- Ph.D Candidate in Microbiology, Ayat Ollah Amoli Branch, Islamic Azad University, Amol, Iran
4- Associate Professor, Department of Pathology, Golestan Univercity of Medical Sciences, Gorgan, Iran
5- Assistant Professor, Department of Pathology, Golestan Univercity of Medical Sciences, Gorgan, Iran
6- Assistant Professor, Department of Pathology, Golestan Univercity of Medical Sciences, Gorgan, Iran , vahidehkazeminejad@yahoo.com
Abstract:   (12597 Views)

Background and Objective: Helicobacter pylori are one of the most common gastrointestinal bacterium infections. This study was done to compare the urease test with Giemsa staining to detection of Helicobacter pylori infection in patients with gastric ulcer and gastritis.

Methods: This descriptive – analytic study was conducted on 601 patients' diagnosed with gastric ulcer and gastritis, whom under went endoscopy in 5th Azar hospital, Gorgan, Iran during 2008-12. Rapid urease test was immediately taken during endoscopy. Pathological examination, Giemsa staining on paraffin blocks and haematoxylin and eosin stain were done to assess either presence or absence of Helicobacter pylori.

Results: Based on biopsy results, Gastritis and gastric ulcer were diagnosed in 80.69% and 19.3%, respectively. In gastritis and gastric ulcer patients, there was a significant differences between urease test (91.5% and 90%, respectively) in comparison with Giemsa staining (91.5% and 90%, respectively) (P<0.05).

Conclusion: The study revealed that in primary diagnosis and screening of role of Helicobacter pylori in patients with gastric ulcer and gastritis, urease test is suitable than giemsa staining.

Keywords: Helicobacter pylori, Gastritis, Gastric ulcer, Giemsa staining, Urease test
Full-Text [PDF 228 kb] [English Abstract]   (12404 Downloads) |   |   Abstract (HTML)  (916 Views)  
Type of Study: Original Articles | Subject: Internal Medicine
References
1. Talebi Bezmin Abadi A, Mohabati Mobarez A, Taghvaei T. [An investigation of the prevalence of iceA genotypes in Helicobacter pylori strains isolated from peptic ulcer patients in Sari (2008)]. Arak Medical University Journal. 2010; 13(3): 84-90. [Article in Persian]
2. Nguyen LT, Uchida T, Murakami K, Fujioka T, Moriyama M. Helicobacter pylori virulence and the diversity of gastric cancer in Asia. J Med Microbiol. 2008 Dec; 57(Pt 12): 1445-53. doi:10.1099/jmm.0.2008/003160-0
3. Khalili Alam K, Kadivar M, Zare Mirzaie A. [Comparing three types of staining methods: Hematoxylin & Eosin, Giemsa, and Hematoxylin & Eosin with Prolonged Hematoxylin time in Detection of Helicobacter Pylori in gastric biopsies of patients with chronic gastritis]. Razi Journal of Medical Sciences. 2006; 12(49): 39-44. [Article in Persian]
4. Brawnwald E, Fauci A, Kasper D, Hauser S, Longo D, Jameson J. Harrison’s principles of internal medicine. 11th ed. New York: McGraw-Hill book Company. 2001; pp: 1995-99.
5. Adeniyi BA, Otegbayo JA, Lawal TO, Oluwasola AO, Odaibo GN, Okolo C, et al. Prevalence of Helicobacter pylori infection among dyspepsia patients in Ibadan, South West Nigeria. African Journal of Microbiology Research. 2012; 6(14): 3399-402. doi:10.5897/AJMR11.1111
6. Rosenstock SJ, Jørgensen T, Bonnevie O, Andersen LP. Does Helicobacter pylori infection explain all socio-economic differences in peptic ulcer incidence? Genetic and psychosocial markers for incident peptic ulcer disease in a large cohort of Danish adults. Scand J Gastroenterol. 2004 Sep; 39(9): 823-9.
7. Rosenstock S, Jørgensen T, Bonnevie O, Andersen L. Risk factors for peptic ulcer disease: a population based prospective cohort study comprising 2416 Danish adults. Gut. 2003; 52(2): 186-93.
8. Rasool S, Abid S, Iqbal MP, Mehboobali N, Haider G, Jafri W. Relationship between vitamin B12, folate and homocysteine levels and H. Pylori infection in patients with functional dyspepsia: A cross-section study. BMC Res Notes. 2012; 5: 206. doi:10.1186/1756-0500-5-206
9. Tijjani B, Umar A. Peptic ulcer disease and helicobacter pylori infection at kano, Nigeria. The Internet Journal of Gastroenterology. 2009; 8(1): 55-63.
10. Buzás GM, Lotz G, Schneider F, Józan J. Changing prevalence of Helicobacter pylori infection in the 9th district of Budapest. A retrospective endoscopic study, 1997–2012. Orvosi Hetilap. 2013; 154(23): 900-907. http://dx.doi.org/10.1556/OH.2013.29630
11. Goudarzi H, Rezaee H, Rafizadeh M, Mirsamadi E, Mirsamadi A. [The frequency of cagA gene of H.pylori isolated from biopsy specimen in Tehran during 2008-2010]. Arak Medical University Journal. 2012; 15(5): 42-48. [Article in Persian]
12. Kargar M, Baghernejad M, Doosti A. [Compression of three methods of polymerase chain reaction, culture and rapid urease test in diagnosis of Helicobactr pylori in gastric biopsy specimen]. Koomesh. 2010; 11(3): 198-203. [Article in Persian]
13. Romshoo GhJ, Malik GM, Basu JA, Bhat MY, Khan AR. Prevalence of Helicobacter Pylori infection in peptic ulcer patients of highly endemic Kashmir Valley. Diagn Ther Endosc. 1999; 6(1): 31-36. doi:10.1155/DTE.6.31
14. McJunkin B, Sissoko M, Levien J, Upchurch J, Ahmed A. Dramatic decline in prevalence of Helicobacter pylori and peptic ulcer disease in an endoscopy-referral population. Am J Med. 2011 Mar; 124(3): 260-4. doi:10.1016/j.amjmed.2010.11.013
15. Seyedeyn Khorasani M, Yousefi Mashouf R, Majlesi A, Jaefari M, Alikhani MY. [Compression of histopathology, culture and rapid urease test in diagnosis of Helicobacter pylori in gastric biopsy Specimens]. Medical Laboratory Journal. 2015; 9(1): 32-38. [Article in Persian]
16. Fakhrjou A, Somi MH, Fattahi E, Koohbanani SS, Shadravan S. Rapid urease test, touch cytology and histopathologic assessment in determining infection by Helicobacter pylori in outpatient setting. Pak J Biol Sci. 2011 Jun; 14(12): 698-702.
17. Hashemi MR, Rahnavardi M, Bikdeli B, Dehghani Zahedani M, Iranmanesh F. Touch cytology in diagnosing Helicobacter pylori: comparison of four staining methods. Cytopathology. 2008 Jun; 19(3): 179-84. doi:10.1111/j.1365-2303.2007.00417.x
18. Vahedi H, Sohrabi MB, Zolfaghari P, Dashtipour M, Yarmohammadi M, Yahyaei E, et al. [Comparison of serological and biopsy diagnostic tests for Helicobacter pylori in dyspeptic patients]. Journal of Knowledge & Health. 2015; 10(1): 37-43. doi:10.1234/knh.v10i1.473 [Article in Persian]
19. Lee JY, Kim N. Diagnosis of Helicobacter pylori by invasive test: histology. Ann Transl Med. 2015 Jan; 3(1): 10. doi:10.3978/j.issn.2305-5839.2014.11.03
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Davaloo R, Kaboosi H, Heidari K, Azarhoush R, Naeimi-Tabiei E, Kazeminejad V. Compraision of urease test with Giemsa staining for detection of Helicobacter pylori infection in patients with gastric ulcer and gastritis. J Gorgan Univ Med Sci 2017; 19 (1) :67-71
URL: http://goums.ac.ir/journal/article-1-3019-en.html


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Volume 19, Issue 1 (3-2017) Back to browse issues page
مجله دانشگاه علوم پزشکی گرگان Journal of Gorgan University of Medical Sciences
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