[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Home::
Journal Information::
About the Journal
Aim and Scope
Content Coverage
Ethics
Conflict of Interest
Copyrights & License
Price
Indexing Sources::
Editorial Board::
Executive Members::
Articles Archive::
Current Issue
All Issues
Instruction to Authors::
Peer-Review::
Contact Us::
Contact Us
Contact Form
Site Facilities::
Search contents
::
Search in website

Advanced Search
Receive site information
Enter your Email in the following box to receive the site news and information.
:: Volume 18, Issue 4 (12-2016) ::
J Gorgan Univ Med Sci 2016, 18(4): 36-41 Back to browse issues page
Effect of Hyssopus officinalis leaves alcoholic extract on motor neuron density in anterior horn after sciatic nerve compression in rats
M Tehranipour * 1, A Lagzian2
1- Associate Professor of Animal Physiology, Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran , maryam_tehranipour@mshdiau.ac.ir
2- B.Sc in Biochemistry, Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran
Abstract:   (10760 Views)

Background and Objective: The degeneration of motor neuron in anterior horn of spinal cord can be caused by compression. Hyssopus officinalis of Laminacea family demonstrate antioxidant and anti-inflammation effects. This study was done to evaluate the effect of alcoholic extract of Hyssopus officinalis leaves, on motor neuron in spinal cord after sciatic nerve compression in male rats.

Methods: In this experimental research, 60 male wistar rats were randomly allocated into six groups including; control, compression, and compression + treatment (25, 50, 75, 100 mg/kg/bw). In order to induce compression, sciatic nerve of right leg was exposed to compression for 60 second using locker pincers. Extract injected intraperitoneally in the first and second week after compression. 28 days after compression under profusion method, the lumber spinal cord was sampled. The density of motor neurons (9-20 micron) was measured using dissector and stereological method.

Results: Density of neurons in compression group (611±34) significantly reduced compared to the control group (1658±30) (P<0.05). Moreover, neuronal density was significantly increased in
25 (1179±22), 50 (1260±20), 75 (1350±15) and 100 (1120±10) mg/kg/bw doses in treatment groups in compared to the compression group (P<0.05).

Conclusion: Alcoholic extract of Hyssopus officinalis leaves exhibite neuroprotective effect on neurons in anterior horn of the spinal cord after injury. This effect probably is related to the antioxidant and anti inflammation properties in alcoholic extract of Hyssopus officinalis, dose dependly.
Keywords: Hyssopus officinalis, Neuron, Sciatic nerve, Stereolog, Rat
Full-Text [PDF 274 kb] [English Abstract]   (12807 Downloads) |   |   Abstract (HTML)  (1129 Views)  
Type of Study: Original Articles | Subject: Neurosciences
References
1. Kerschensteiner M, Schwab ME, Lichtman JW, Misgeld T. In vivo imaging of axonal degeneration and regeneration in the injured spinal cord. Nat Med. 2005 May; 11(5): 572-7.
2. Guertin AD, Zhang DP, Mak KS, Alberta JA, Kim HA. Microanatomy of axon/glial signaling during Wallerian degeneration. J Neurosci. 2005 Mar; 25(13): 3478-87.
3. Maleki N, Garjani A, Nazemiyeh H, Nilfouroushan N, Eftekhar Sadat AT, Allameh Z, et al. Potent anti-inflammatory activities of hydroalcoholic extract from aerial parts of Stachys inflata on rats. J Ethnopharmacol. 2001 May; 75(2-3): 213-8.
4. Vargas ME, Barres BA. Why is Wallerian degeneration in the CNS so slow? Annu Rev Neurosci. 2007; 30: 153-79.
5. Tehranipour M, Samare- Mousavi S. [Investigating neuroprotective effect of leave of solanum Nigrum L Ethyl acetate fraction on motoneurons degeneration rate in rats]. J Shahid Sadoughi Univ Med Sci. 2013; 21 (2):197-207. [Article in Persian]
6. Tehranipour M, Javadmoosavi BZ, Kehtarpour M, Khayyatzade J. [Effect of aquatic extract of Cannabis sativa leaves on degeneration of alpha motoneurons in spinal cord after sciatic nerve compression in Rats]. J Gorgan Uni Med Sci. 2011; 13(1): 16-22. [Article in Persian]
7. Alikhanzade M, Tehranipour M, Khayatzade J. [The study of effect of aquatic extracts of Achillea biebersteinii leave on repair alpha motoneurons after sciatic nerve injury in rat]. J Shahrekord Univ Med Sci. 2013; 15(4): 16-25. [Article in Persian]
8. Ferdosi- Makan M, Khayatzadeh J, Tehranipour M, Behnam Rasouli M. [Investigating N-Butanol and Ethyl Acetate fractions of Nigella Sativa on motoneurons’ density of spinal cord ventral horn in rats with compressived injury of sciatic nerve]. J Shahid Sadoughi Univ Med Sci. 2015; 22(6): 1612-22. [Article in Persian]
9. Lawless J. The encyclopedia of essential oils. The complete guide to the use of aromatic oils in aromatherapy, herbalism, health & well-being. 1st ed. Massachusetts: Conari Press. 2013; pp: 110-11.
10. Dehghanzadeh N, Ketabchi S, Alizadeh A. Essential Oil composition and antibacterial activity of Hyssop Officinalis L. grown in Iran. Asian J Exp Biol Sci. 2012; 3(4): 767-71.
11. Fathiazad F, Mazandarani M, Hamedeyazdan S. Phytochemical analysis and antioxidant activity of Hyssopus officinalis L. from Iran. Adv Pharm Bull. 2011; 1(2): 63-7. doi:10.5681/apb.2011.009
12. Glamoclija JM, Sokovic MD, Vukojevic JB, Milenkovic IM, Brkic DD, Griensven LJLD. Antifungal activity of essential oil of Hyssopus officinalis L. against mycopathogen Mycogone perniciosa (Mang). Zbornik Matice srpske za prirodne nauke. 2005; 109: 123-28. doi:10.2298/ZMSPN0519123G
13. Vlase L, Benedec D, Hanganu D, Damian G, Csillag I, Sevastre B, et al. Evaluation of antioxidant and antimicrobial activities and phenolic profile for Hyssopus officinalis, Ocimum basilicum and Teucrium chamaedrys. Molecules. 2014 Apr; 19(5): 5490-507. doi:10.3390/molecules19055490
14. Cicchetti E, Chaintreau A. Comparison of extraction techniques and modeling of accelerated solvent extraction for the authentication of natural vanilla flavors. J Sep Sci. 2009 Jun; 32(11): 1957-64. doi:10.1002/jssc.200800650
15. Tehranipour M, Ghadamyari T. The effects of root Aquatic extract of Salvia staminea on neuronal density of alpha motoneurons in spinal cord anterior horn after sciatic nerve compression in rats. Journal of Biological Sciences. 2010; 10: 48-52. doi:10.3923/jbs.2010.48.52
16. Behnam-Rasoli M, Nikravesh M, Mahdavi-Shahri N, Tehranipour M. Post-operative time effects after sciatic nerve crush on the number of alpha motoneurons, using a stereological counting method. Iran Biomed J. 2000; 4(1): 45-49.
17. Tehranipour M, Khayyatzade J, Javaheri fard R. [The protective effects of curcuma longa total extract on spinal cord neuroglia cell degeneration after the sciatic nerve compression in rats]. J Arak Uni Med Sci. 2010; 13(1): 83-89. doi:10.3923/jbs.2009.692.696 [Article in Persian]
18. Tehranipour M, Kabiri M. The effect of exogenous testosterone administration on peripheral nerves regeneration after sciatic nerve compression in rat. Journal of Biological Sciences. 2009; 9(7): 692-96.
19. Hanz S, Fainzilber M. Retrograde signaling in injured nerve--the axon reaction revisited. J Neurochem. 2006 Oct; 99(1): 13-9.
20. Dahlin LB, Brandt J. Basic science of peripheral nerve repair: Wallerian degeneration/growth cones. Oper Tech Orthop. 2004 Jul; 14(3): 138-45.
21. Özdemir ÜS, Nazıroğlu M, Şenol N, Ghazizadeh V. Hypericum perforatum attenuates spinal cord injury-induced oxidative stress and apoptosis in the dorsal root ganglion of rats: involvement of TRPM2 and TRPV1 channels. Mol Neurobiol. 2016 Aug; 53(6): 3540-51. doi:10.1007/s12035-015-9292-1
22. Mechoulam R, Shohami E. Endocannabinoids and traumatic brain injury. Mol Neurobiol. 2007 Aug; 36(1): 68-74.
Send email to the article author

XML   Persian Abstract   Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Tehranipour M, Lagzian A. Effect of Hyssopus officinalis leaves alcoholic extract on motor neuron density in anterior horn after sciatic nerve compression in rats. J Gorgan Univ Med Sci 2016; 18 (4) :36-41
URL: http://goums.ac.ir/journal/article-1-2953-en.html
Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 18, Issue 4 (12-2016) Back to browse issues page
مجله دانشگاه علوم پزشکی گرگان Journal of Gorgan University of Medical Sciences
Persian site map - English site map - Created in 0.05 seconds with 36 queries by YEKTAWEB 4657