[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Home::
Journal Information::
Indexing Sources::
Editorial Board::
Executive Members::
Articles Archive::
Instruction to Authors::
Peer-Review::
Contact Us::
Site Facilities::
::
Search in website

Advanced Search
Receive site information
Enter your Email in the following box to receive the site news and information.
:: Volume 18, Issue 2 (6-2016) ::
J Gorgan Univ Med Sci 2016, 18(2): 115-119 Back to browse issues page
Antibiotic resistance in Staphylococcus aureus isolated from clinical samples by disk diffusion and PCR methods
Kavoosinezhad F1 , Fattahi E * 2, Moori Bakhtiari N3
1- Microbiologist, Department of Biology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran
2- Assistant Professor, Department of Biology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran , esmail_fattahy@yahoo.com
3- Assistant Professor, Department of Pathobiology, Shahid Chamran University of Ahvaz, Ahvaz, Iran
Abstract:   (8781 Views)

Background and Objective: Resistance of Staphylococcus aureus to antibiotics is one of the major global health problems in human societies. Thus, evaluation of pattern of antibiotic resistance in its different strains is very important. This study was carried out to evaluate the antibiotic resistance in Staphylococcus aureus isolated from clinical samples by disk diffusion and PCR methods.

Methods: In this laboratory- descriptive study, 50 isolates of Staphylococcus aureus to be identified from clinical specimens. Methicillin resistance was examined using PCR and antibiotic susceptibility of isolates was tested by disk diffusion method.

Results: 50 isolates were resistant to methicillin, ampicillin and penicillin. The resistance of isolates to erythromycin, Gentamicin, Clindamycin and Ciprofloxacin were 48%, 34%, 34%, 34%, respectively. The PCR method showed that 98% of Methicillin Resistance of Staphylococcus aureus isolates carried the methicillin resistant gene.

Conclusion: This study indicated that 98% isolates harbor mecA genes and more resistant to methicillin related mecA genes.

Keywords: Staphylococcus Aureus, Antibiotic Resistance, Disk diffusion method, PCR
Full-Text [PDF 283 kb] [English Abstract]   (13953 Downloads) |   |   Abstract (HTML)  (600 Views)  
Type of Study: Original Articles | Subject: Microbiology
References
1. Bronner S, Monteil H, Prévost G. Regulation of virulence determinants in Staphylococcus aureus: complexity and applications. FEMS Microbiol Rev. 2004 May; 28(2): 183-200.
2. Novick RP, Schlievert P, Ruzin A. Pathogenicity and resistance islands of staphylococci. Microbes Infect. 2001 Jun; 3(7): 585-94.
3. Silva EC, Antas Md, Monteiro B Neto A, Rabelo MA, Melo FL, Maciel MA. Prevalence and risk factors for Staphylococcus aureus in health care workers at a university hospital of Recife-PE. Braz J Infect Dis. 2008 Dec; 12(6): 504-8.
4. Masomi J. Yadegari D, Mozani Sh. [More appropriate antimicrobial agents for antibiogram]. Iran J Infect Dis Trop Med. 2005; 10 (29): 53-58. [Article in Persian]
5. Bagherzadeh Yazdchi S, Pourmand M, Hajiabdolbaghi M, Hoseini M, Mardani N. [Molecular characterization of hypervariable region (hvr) and antibiotic susceptibility patterns of Staphylococcus aureus strains isolates collected from Tehran University of Medical Sciences Hospitals]. J Sch Public Health Inst Public Health Res. 2008; 6(2): 39-47. [Article in Persian]
6. Vinodhkumaradithyaa A, Uma A, Shirivasan M, Ananthalakshmi I, Nallasivam P, Thirumalaikolundusubramanian P. Nasal carriage of methicillin-resistant Staphylococcus aureus among surgical unit staff. Jpn J Infect Dis. 2009 May;62(3):228-9.
7. Bernal P, Lemaire S, Pinho MG, Mobashery S, Hinds J, Taylor PW. Insertion of epicatechin gallate into the cytoplasmic membrane of methicillin-resistant Staphylococcus aureus disrupts penicillin-binding protein (PBP) 2a-mediated beta-lactam resistance by delocalizing PBP2. J Biol Chem. 2010 Jul; 285(31): 24055-65. doi:10.1074/jbc.M110.114793
8. Pinho MG, de Lencastre H, Tomasz A. An acquired and a native penicillin-binding protein cooperate in building the cell wall of drug-resistant staphylococci. Proc Natl Acad Sci U S A. 2001 Sep; 98(19): 10886-91.
9. Shehab el-din SA, El-shfey ElI, El-hadiy MR, El- dlin AB, El-hadiy MM, Zaghlvul HA. Methicillin resistant staphylococcus aureus, a problem in the burns unit. Egypt J Plast Reconstr Surg. 2003; 27(1): 1-10.
10. Rahimi F, Arabestani MR. [Enterotoxin a producing Methicillin resistant Staphylococcus aureus in profiteroles]. Iran J Infect Dis Trop Med. 2014; 19(2): 53-58. [Article in Persian]
11. Lowy FD. Staphylococcus aureus infections. N Engl J Med. 1998 Aug; 339(8): 520-32.
12. Merlino J, Watson J, Rose B, Beard-Pegler M, Gottlieb T, Bradbury R, et al. Detection and expression of methicillin/oxacillin resistance in multidrug-resistant and non-multidrug-resistantStaphylococcus aureus in Central Sydney, Australia. J Antimicrob Chemother. 2002 May; 49(5): 793-801.
13. Sakoulas G, Gold HS, Venkataraman L, DeGirolami PC, Eliopoulos GM, Qian Q. Methicillin-resistant Staphylococcus aureus: comparison of susceptibility testing methods and analysis of mecA-positive susceptible strains. J Clin Microbiol. 2001 Nov; 39(11): 3946-51.
14. Jenison R, Haeberli A, Yang S, Polisky B, Ostroff R. Thin film biosensor for rapid detection of mecA from methicillin-resistant staphylococcus aureus. Clin Chem. 2000 Sep; 46(9): 1501-4.
15. Clinical and Laboratory Standard Institute. Performance standards for antimicrobial susceptibility testing. 16th informational supplement. Clinical and Laboratory Standard Institute, Wayne, Pa. 2006.
16. Sambrook J, Russell D. Molecular cloning, a laboratory Manual. 3rd ed. New York: Cold Spring Harbor Laboratory Press. 2001; pp: 8.4-8.5.
17. Rahimi-Alang S, Asmar M, Cheraghali F, Yazarlou S, Amini A, Shakeri F, et al. [Frequency of methicillin resistant Staphylococcus aureus in health care]. Zahedan J Res Med Sci. 2011; 13(1): 17-22. [Article in Persian]
18. Shokri R, Salouti M, Sorouri Zanjani R, Heidari Zh. [Frequency of meticillin resistant Staphylococcus aureus strains isolated from clinical samples in Mousavi Hospital, Zanjan, and recognition mec Agene using PCR]. Journal of Microbial World. 2014; 7(1): 58-65. [Article in Persian]
19. Darabi N, Habibollahi H, Shahbabian K. [Molecular epidemiology of Staphylococcus aureus isolated from patients and personnel in Army hospital]. Ann Mil Health Sci Res. 2010; 8(3): 193-99. [Article in Persian]
20. Rezazadeh M, Yousefi Mashouf R, Sarmadyan H, Ghaznavi-Rad E. [Antibiotic profile of Methicillin-resistant Staphylococcus aureus with multiple-drug resistances isolated from nosocomial infections in Vali-Asr Hospital of Arak]. J Arak Univ Med Sci. 2013;16(2): 29-37. [Article in Persian]
21. Zamani A, Sadeghian S, Najafi Mosleh M, Goodarzi MT, Yousefi Mashouf R, Ghaderkhani J. [Detection of Methicillin-resistance gene (mec-A) in Staphylococcus aureus strains by PCR and determination of antibiotic sensitivity]. Sci J Hamadan Univ Med Sci. 2007;14(3): 54-58. [Article in Persian]
22. Sader HS, Jones RN, Dowzicky MJ, Fritsche TR. Antimicrobial activity of tigecycline tested against nosocomial bacterial pathogens from patients hospitalized in the intensive care unit. Diagn Microbiol Infect Dis. 2005 Jul; 52(3): 203-8.
Send email to the article author


XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Kavoosinezhad F, Fattahi E, Moori Bakhtiari N. Antibiotic resistance in Staphylococcus aureus isolated from clinical samples by disk diffusion and PCR methods. J Gorgan Univ Med Sci 2016; 18 (2) :115-119
URL: http://goums.ac.ir/journal/article-1-2771-en.html


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 18, Issue 2 (6-2016) Back to browse issues page
مجله دانشگاه علوم پزشکی گرگان Journal of Gorgan University of Medical Sciences
Persian site map - English site map - Created in 0.05 seconds with 36 queries by YEKTAWEB 4652