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:: Volume 18, Issue 2 (6-2016) ::
J Gorgan Univ Med Sci 2016, 18(2): 115-119 Back to browse issues page
Antibiotic resistance in Staphylococcus aureus isolated from clinical samples by disk diffusion and PCR methods
Kavoosinezhad F1 , Fattahi E * 2, Moori Bakhtiari N3
1- Microbiologist, Department of Biology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran
2- Assistant Professor, Department of Biology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran , esmail_fattahy@yahoo.com
3- Assistant Professor, Department of Pathobiology, Shahid Chamran University of Ahvaz, Ahvaz, Iran
Abstract:   (8781 Views)

Background and Objective: Resistance of Staphylococcus aureus to antibiotics is one of the major global health problems in human societies. Thus, evaluation of pattern of antibiotic resistance in its different strains is very important. This study was carried out to evaluate the antibiotic resistance in Staphylococcus aureus isolated from clinical samples by disk diffusion and PCR methods.

Methods: In this laboratory- descriptive study, 50 isolates of Staphylococcus aureus to be identified from clinical specimens. Methicillin resistance was examined using PCR and antibiotic susceptibility of isolates was tested by disk diffusion method.

Results: 50 isolates were resistant to methicillin, ampicillin and penicillin. The resistance of isolates to erythromycin, Gentamicin, Clindamycin and Ciprofloxacin were 48%, 34%, 34%, 34%, respectively. The PCR method showed that 98% of Methicillin Resistance of Staphylococcus aureus isolates carried the methicillin resistant gene.

Conclusion: This study indicated that 98% isolates harbor mecA genes and more resistant to methicillin related mecA genes.

Keywords: Staphylococcus Aureus, Antibiotic Resistance, Disk diffusion method, PCR
Full-Text [PDF 283 kb] [English Abstract]   (13953 Downloads) |   |   Abstract (HTML)  (600 Views)  
Type of Study: Original Articles | Subject: Microbiology
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Kavoosinezhad F, Fattahi E, Moori Bakhtiari N. Antibiotic resistance in Staphylococcus aureus isolated from clinical samples by disk diffusion and PCR methods. J Gorgan Univ Med Sci 2016; 18 (2) :115-119
URL: http://goums.ac.ir/journal/article-1-2771-en.html

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Volume 18, Issue 2 (6-2016) Back to browse issues page
مجله دانشگاه علوم پزشکی گرگان Journal of Gorgan University of Medical Sciences
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